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The map label for this gene is ech [C]

Identifier: 190895248

GI number: 190895248

Start: 1009382

End: 1010206

Strand: Reverse

Name: ech [C]

Synonym: RHECIAT_PC0000921

Alternate gene names: 190895248

Gene position: 1010206-1009382 (Counterclockwise)

Preceding gene: 190895249

Following gene: 190895247

Centisome position: 92.55

GC content: 62.06

Gene sequence:

>825_bases
ATGACTGAAAACACATCGCCAGTCCTGGTCGAATTCGATGGCGGCATCGCCTTCGTGACGCTGAACCGTCCGGAAAAGCG
CAATGCGATGAACCCGGCGCTCAATGCCCGGATGCTCGAGGTGCTCGACGAACTCGAAGGCGACGAGCGCTGCGGCGTTC
TCGTGCTGCGCGGCGCCGGCCAATCCTGGTCGGCCGGCATGGATCTGAAGGAATATTTCCGCGACAACGACGACAAGCCG
CGCGACGCCACGCTGAAGGCGCGGCGCCAATCCGGCGGCTGGTGGGGCCGGCTGATGTATTTCGAAAAGCCGACGATCGC
CATGGTCAACGGCTGGTGCTTCGGCGGTGCCTTCACGCCGCTCGTCTCCTGCGATCTCGCCATTGCTGCCGAGGAAGCGA
ATTTCGGCCTCTCCGAGATCAATTGGGGCATCCTGCCGGGCGGCAACGTCACCCGTGCGGTGGCCGAGGTGATGCGCCAT
CGCGATGCCCTCTATTACATCATGACCGGCGAATTGTTCGGTGGGCGCAAGGCTGCCGAAATGGGCCTCGTCAACGAGGC
CGTGCCGCTGGTCGACCTTGAAACCCGTGTCCGCAAGATCTGCGCCAGCCTGCTCGAAAAGAACCCGGTAACGCTGAAGG
CTGCCAAGGACACCTACAAGCGCGTGCGCAACCTGCCGTGGGATCTTGCTGACGACTACATCTACGCCAAGCTGGAGCAG
ATGCTGTTTCTCGACAAGACCAAGGGGCGCGATGAAGGGCTGAAACAGTTCCTCGATGACAAGACCTATCAGCCGGGACT
CGGCGCTTACAAGCGCGGCCGTTGA

Upstream 100 bases:

>100_bases
GAAACATGTTCTGCGCCAGCACCATTCCGAAGAAATCCTTCAACGACAGGCCATGTGAAGCGGCTGCGGCCAATCACGCC
GAAACCAATGGAGTGAACCT

Downstream 100 bases:

>100_bases
GGTCAGGCCTGGCATGGCCGGGCCGTGACGGATTGCTATCGGGAGGAGGAAGCAATGAAGATTCACGCCGCGGTGGCGCG
TGCGCCGCATATGCCGCTTT

Product: p-hydroxycinnamoyl CoA hydratase/lyase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTENTSPVLVEFDGGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAGQSWSAGMDLKEYFRDNDDKP
RDATLKARRQSGGWWGRLMYFEKPTIAMVNGWCFGGAFTPLVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRH
RDALYYIMTGELFGGRKAAEMGLVNEAVPLVDLETRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYKRGR

Sequences:

>Translated_274_residues
MTENTSPVLVEFDGGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAGQSWSAGMDLKEYFRDNDDKP
RDATLKARRQSGGWWGRLMYFEKPTIAMVNGWCFGGAFTPLVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRH
RDALYYIMTGELFGGRKAAEMGLVNEAVPLVDLETRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYKRGR
>Mature_273_residues
TENTSPVLVEFDGGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAGQSWSAGMDLKEYFRDNDDKPR
DATLKARRQSGGWWGRLMYFEKPTIAMVNGWCFGGAFTPLVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRHR
DALYYIMTGELFGGRKAAEMGLVNEAVPLVDLETRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQM
LFLDKTKGRDEGLKQFLDDKTYQPGLGAYKRGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=26.9076305220884, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=26.4822134387352, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI70995211, Length=138, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI45643119, Length=217, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260275230, Length=217, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260274832, Length=217, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=217, Percent_Identity=25.3456221198157, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI213417737, Length=217, Percent_Identity=25.3456221198157, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787659, Length=262, Percent_Identity=31.6793893129771, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788597, Length=208, Percent_Identity=32.2115384615385, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI221142681, Length=225, Percent_Identity=30.2222222222222, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082183, Length=276, Percent_Identity=25.3623188405797, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787660, Length=181, Percent_Identity=29.2817679558011, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=26.6304347826087, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=26.6932270916335, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=28.2352941176471, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=28.3653846153846, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=28.0373831775701, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=199, Percent_Identity=27.6381909547739, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=235, Percent_Identity=23.8297872340426, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=247, Percent_Identity=23.4817813765182, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=29.2553191489362, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=29.2553191489362, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=29.2553191489362, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=217, Percent_Identity=25.3456221198157, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=26.3681592039801, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=201, Percent_Identity=23.8805970149254, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129971, Length=174, Percent_Identity=28.735632183908, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653477, Length=174, Percent_Identity=28.735632183908, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=29.4776119402985, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357171, Length=184, Percent_Identity=30.4347826086957, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=27.9693486590038, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=243, Percent_Identity=27.5720164609054, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583165, Length=279, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920382, Length=213, Percent_Identity=25.8215962441315, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30712; Mature: 30580

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENTSPVLVEFDGGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAG
CCCCCCCEEEEECCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCC
QSWSAGMDLKEYFRDNDDKPRDATLKARRQSGGWWGRLMYFEKPTIAMVNGWCFGGAFTP
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEEECCCEEEEECEEECCHHHH
LVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRHRDALYYIMTGELFGGRKAAE
HHHCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
MGLVNEAVPLVDLETRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
HHHHHHCCCCEEHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHH
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYKRGR
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TENTSPVLVEFDGGIAFVTLNRPEKRNAMNPALNARMLEVLDELEGDERCGVLVLRGAG
CCCCCCEEEEECCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCC
QSWSAGMDLKEYFRDNDDKPRDATLKARRQSGGWWGRLMYFEKPTIAMVNGWCFGGAFTP
CCCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCEEEEEEEEECCCEEEEECEEECCHHHH
LVSCDLAIAAEEANFGLSEINWGILPGGNVTRAVAEVMRHRDALYYIMTGELFGGRKAAE
HHHCCEEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHH
MGLVNEAVPLVDLETRVRKICASLLEKNPVTLKAAKDTYKRVRNLPWDLADDYIYAKLEQ
HHHHHHCCCCEEHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHH
MLFLDKTKGRDEGLKQFLDDKTYQPGLGAYKRGR
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA