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The map label for this gene is ilvB [H]

Identifier: 190895133

GI number: 190895133

Start: 863494

End: 865296

Strand: Reverse

Name: ilvB [H]

Synonym: RHECIAT_PC0000803

Alternate gene names: 190895133

Gene position: 865296-863494 (Counterclockwise)

Preceding gene: 190895134

Following gene: 190895132

Centisome position: 79.27

GC content: 60.23

Gene sequence:

>1803_bases
ATGAAACTCACAGGTGGCCAGGTCGTCGCAAGGGCACTGAAAGAGTATGGCGTTGAATATGTGGCTGGCGTGCCGGGGCA
CGGGATCTGGGCGCTTTTCGACGCATTCCTTGAAGAAGGCTCCCAACTTCCGTTCATCCAGGTCATGCACGAGCAGAGCG
CCGTTCATATGGCCGATGGATATTTCCGAGCCAGCGGAAAGCCGATGGCTTGCTCGACCTCTGTCGGGCCGGGCGCTGCA
AATACCGTCATCGGCCTCGCAACGGCGCTGACTGATTCCACCTCGCTCTTCTACGTATCTGGCTCTCCGCAGACCTACAT
GCACGGCCACGGCACCATGCAGGAACTCGAGCGTCATCAGGATAACGCCTTCCCGCGCGTCACCGAGCAGGTTACCAAGC
GTGCGTGGCAGGCAAATTCCGTTCAGGTTCTTCCCAGCATCATGCATCGCGCATTCAGCGAGATGCTGACCGGCCGTCCG
GGTCCGGTGCACGTCGAAGTGCCTATGGACGTTCAAGTCGAAGCTGCTGACGTCACGATCCATCCGCTCCAAAAGCGGCT
TCCGATCGGCATTGCCTACCCGGATCCGCGTGCAATCGAAGCTGCAGTGAAGGTCCTTCTCGCAGCCGAGCGACCTGTCA
TTGTCGCTGGCGGCGGTGCTATTTCTGCGAATGCGTCGGAGGAATTGACGCGGCTGGCTGAAAAACTCGGTGCGGCCGTG
TCGATCACCTGGAACGGCAAGGGTGCAATCTCTGAAGATCACCAGCTGTTCATCGGGGCCGTCGGCCAGACCGGCACCAC
GTGCGGCAATAAAATTACGGCTTCGGCAGACGTCGTCGTCTCGGTCGGTTGTCGCTTCACGGATTGGTCCGCATCCTCCT
ATGCCAAGGGCGTCTCGTTCTCGATTCCCTCTGCGAAGCTGATCCACATCGATCTCGATCCACGCGAAATCGGCAAGAAC
TACGAGACCGAAGTCGGCATCGTCGCGGACGCCAAGGTCACGCTTGAAGCCATCCTGTCGCTGATTTCCGACGCAGACTC
CACAAACATGCTGTCGCGCCGAGAGAATTTCCTGGCTGATGTACAGAAGGCGAAGGCAGATTGGCGCGCCCAGGTCGAGC
CGCGTGAGACGAGCCGGGAAACGCCGTTCACGTCGCAGCGCCCCTTGATGGCGCTTCGCAAGGTGCTCGACCGTGACGGC
ATCGTCGTCGTCGGCTCGGGGAATACGCAAGGCTCCGTCAAGCAGAGCTTCCCGGTCTACAAGCCTCGCACGCACATCAC
GTCCGGCTCCTACTCGCCGATGGGATGGGCGGTGCCTGCAGCGCTCGGCGCGAAACTGGCTTGCCCGGACAAGCAGGTCG
TCGCGATCGTCGGAGACGGTGATTTCATGATGTCTCTCCCGGAGATGGGAACGGCAGTCATGAACGGCATCAATGTCGTG
TTCCTCGTGCTGAACAATTTCGGATACATGTCCATCCGCGGTGGTCAGCGGAAGTTCATGGGCCGCCACGTCGCTTCCGA
GTTCAACCACCATGTCGGCAACGGTGAACCCTATTCTGCCGACATCGCCGCCTCTGCACGCGCATTTGGTCTCGAAGCAT
GGAAGGTCGAGAAGGACGAGGACCTCGAAAGCAGCATCAAGGCTGCTTTGGAGTGCGGTGGGCCTGCTCTCGTCGAGGTG
ATCGTATCGCGTGACGCCGCTGGCCCGTTCGCCACCGGCTGGTGGGACTTCCCGTCGCCGGCCTATTACGAGAAGGAACA
GGCTGCCTACGCAAAGATGCGCGAACTCGAGCAGCATCTATAG

Upstream 100 bases:

>100_bases
GCGAGTCCGCCCGATCCGGCCGCAATGTAGTCGCAGTTGGGCGGCTGCGGTCATTACCAGCCCACGACTAAAGAACAACG
AACTTTAGAGGGTGAAGAAG

Downstream 100 bases:

>100_bases
AGCCAACTTCAGGCTTGGGGCGCCGCCTGGCGCCCCGCCAACCACAGTCTCTCCAAACCCAGAATGCGGGGCACGGCTTC
CGCAAACGAAGAGTTATTGT

Product: putative acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKLTGGQVVARALKEYGVEYVAGVPGHGIWALFDAFLEEGSQLPFIQVMHEQSAVHMADGYFRASGKPMACSTSVGPGAA
NTVIGLATALTDSTSLFYVSGSPQTYMHGHGTMQELERHQDNAFPRVTEQVTKRAWQANSVQVLPSIMHRAFSEMLTGRP
GPVHVEVPMDVQVEAADVTIHPLQKRLPIGIAYPDPRAIEAAVKVLLAAERPVIVAGGGAISANASEELTRLAEKLGAAV
SITWNGKGAISEDHQLFIGAVGQTGTTCGNKITASADVVVSVGCRFTDWSASSYAKGVSFSIPSAKLIHIDLDPREIGKN
YETEVGIVADAKVTLEAILSLISDADSTNMLSRRENFLADVQKAKADWRAQVEPRETSRETPFTSQRPLMALRKVLDRDG
IVVVGSGNTQGSVKQSFPVYKPRTHITSGSYSPMGWAVPAALGAKLACPDKQVVAIVGDGDFMMSLPEMGTAVMNGINVV
FLVLNNFGYMSIRGGQRKFMGRHVASEFNHHVGNGEPYSADIAASARAFGLEAWKVEKDEDLESSIKAALECGGPALVEV
IVSRDAAGPFATGWWDFPSPAYYEKEQAAYAKMRELEQHL

Sequences:

>Translated_600_residues
MKLTGGQVVARALKEYGVEYVAGVPGHGIWALFDAFLEEGSQLPFIQVMHEQSAVHMADGYFRASGKPMACSTSVGPGAA
NTVIGLATALTDSTSLFYVSGSPQTYMHGHGTMQELERHQDNAFPRVTEQVTKRAWQANSVQVLPSIMHRAFSEMLTGRP
GPVHVEVPMDVQVEAADVTIHPLQKRLPIGIAYPDPRAIEAAVKVLLAAERPVIVAGGGAISANASEELTRLAEKLGAAV
SITWNGKGAISEDHQLFIGAVGQTGTTCGNKITASADVVVSVGCRFTDWSASSYAKGVSFSIPSAKLIHIDLDPREIGKN
YETEVGIVADAKVTLEAILSLISDADSTNMLSRRENFLADVQKAKADWRAQVEPRETSRETPFTSQRPLMALRKVLDRDG
IVVVGSGNTQGSVKQSFPVYKPRTHITSGSYSPMGWAVPAALGAKLACPDKQVVAIVGDGDFMMSLPEMGTAVMNGINVV
FLVLNNFGYMSIRGGQRKFMGRHVASEFNHHVGNGEPYSADIAASARAFGLEAWKVEKDEDLESSIKAALECGGPALVEV
IVSRDAAGPFATGWWDFPSPAYYEKEQAAYAKMRELEQHL
>Mature_600_residues
MKLTGGQVVARALKEYGVEYVAGVPGHGIWALFDAFLEEGSQLPFIQVMHEQSAVHMADGYFRASGKPMACSTSVGPGAA
NTVIGLATALTDSTSLFYVSGSPQTYMHGHGTMQELERHQDNAFPRVTEQVTKRAWQANSVQVLPSIMHRAFSEMLTGRP
GPVHVEVPMDVQVEAADVTIHPLQKRLPIGIAYPDPRAIEAAVKVLLAAERPVIVAGGGAISANASEELTRLAEKLGAAV
SITWNGKGAISEDHQLFIGAVGQTGTTCGNKITASADVVVSVGCRFTDWSASSYAKGVSFSIPSAKLIHIDLDPREIGKN
YETEVGIVADAKVTLEAILSLISDADSTNMLSRRENFLADVQKAKADWRAQVEPRETSRETPFTSQRPLMALRKVLDRDG
IVVVGSGNTQGSVKQSFPVYKPRTHITSGSYSPMGWAVPAALGAKLACPDKQVVAIVGDGDFMMSLPEMGTAVMNGINVV
FLVLNNFGYMSIRGGQRKFMGRHVASEFNHHVGNGEPYSADIAASARAFGLEAWKVEKDEDLESSIKAALECGGPALVEV
IVSRDAAGPFATGWWDFPSPAYYEKEQAAYAKMRELEQHL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=585, Percent_Identity=24.1025641025641, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI21361361, Length=616, Percent_Identity=24.3506493506494, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=30.9608540925267, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=30.1785714285714, Blast_Score=251, Evalue=6e-68,
Organism=Escherichia coli, GI1786717, Length=563, Percent_Identity=31.9715808170515, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=24.1681260945709, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1788716, Length=577, Percent_Identity=25.6499133448873, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=503, Percent_Identity=26.2425447316103, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=503, Percent_Identity=26.2425447316103, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=551, Percent_Identity=22.6860254083485, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=28.6926994906621, Blast_Score=226, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6320816, Length=503, Percent_Identity=25.844930417495, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=566, Percent_Identity=23.4982332155477, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=506, Percent_Identity=23.3201581027668, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=23.9043824701195, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=26.3431542461005, Blast_Score=152, Evalue=5e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64448; Mature: 64448

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTGGQVVARALKEYGVEYVAGVPGHGIWALFDAFLEEGSQLPFIQVMHEQSAVHMADG
CCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECC
YFRASGKPMACSTSVGPGAANTVIGLATALTDSTSLFYVSGSPQTYMHGHGTMQELERHQ
EEECCCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHH
DNAFPRVTEQVTKRAWQANSVQVLPSIMHRAFSEMLTGRPGPVHVEVPMDVQVEAADVTI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECEEE
HPLQKRLPIGIAYPDPRAIEAAVKVLLAAERPVIVAGGGAISANASEELTRLAEKLGAAV
ECHHHCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHCCCEE
SITWNGKGAISEDHQLFIGAVGQTGTTCGNKITASADVVVSVGCRFTDWSASSYAKGVSF
EEEECCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCEEEEECCEEECCCCHHHCCCCEE
SIPSAKLIHIDLDPREIGKNYETEVGIVADAKVTLEAILSLISDADSTNMLSRRENFLAD
ECCCEEEEEEECCHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VQKAKADWRAQVEPRETSRETPFTSQRPLMALRKVLDRDGIVVVGSGNTQGSVKQSFPVY
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCC
KPRTHITSGSYSPMGWAVPAALGAKLACPDKQVVAIVGDGDFMMSLPEMGTAVMNGINVV
CCCCCCCCCCCCCCCCHHHHHHCCEECCCCCEEEEEEECCCCEEECHHHHHHHHCCCEEE
FLVLNNFGYMSIRGGQRKFMGRHVASEFNHHVGNGEPYSADIAASARAFGLEAWKVEKDE
EEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEECCCCC
DLESSIKAALECGGPALVEVIVSRDAAGPFATGWWDFPSPAYYEKEQAAYAKMRELEQHL
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLTGGQVVARALKEYGVEYVAGVPGHGIWALFDAFLEEGSQLPFIQVMHEQSAVHMADG
CCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEEECC
YFRASGKPMACSTSVGPGAANTVIGLATALTDSTSLFYVSGSPQTYMHGHGTMQELERHQ
EEECCCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHHH
DNAFPRVTEQVTKRAWQANSVQVLPSIMHRAFSEMLTGRPGPVHVEVPMDVQVEAADVTI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECEEE
HPLQKRLPIGIAYPDPRAIEAAVKVLLAAERPVIVAGGGAISANASEELTRLAEKLGAAV
ECHHHCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHHHCCCEE
SITWNGKGAISEDHQLFIGAVGQTGTTCGNKITASADVVVSVGCRFTDWSASSYAKGVSF
EEEECCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCEEEEECCEEECCCCHHHCCCCEE
SIPSAKLIHIDLDPREIGKNYETEVGIVADAKVTLEAILSLISDADSTNMLSRRENFLAD
ECCCEEEEEEECCHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VQKAKADWRAQVEPRETSRETPFTSQRPLMALRKVLDRDGIVVVGSGNTQGSVKQSFPVY
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCC
KPRTHITSGSYSPMGWAVPAALGAKLACPDKQVVAIVGDGDFMMSLPEMGTAVMNGINVV
CCCCCCCCCCCCCCCCHHHHHHCCEECCCCCEEEEEEECCCCEEECHHHHHHHHCCCEEE
FLVLNNFGYMSIRGGQRKFMGRHVASEFNHHVGNGEPYSADIAASARAFGLEAWKVEKDE
EEEECCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEECCCCC
DLESSIKAALECGGPALVEVIVSRDAAGPFATGWWDFPSPAYYEKEQAAYAKMRELEQHL
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9099862 [H]