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The map label for this gene is aspA [H]

Identifier: 190895120

GI number: 190895120

Start: 847822

End: 849258

Strand: Reverse

Name: aspA [H]

Synonym: RHECIAT_PC0000790

Alternate gene names: 190895120

Gene position: 849258-847822 (Counterclockwise)

Preceding gene: 190895122

Following gene: 190895100

Centisome position: 77.8

GC content: 57.76

Gene sequence:

>1437_bases
ATGGAAGACGCAGCACTGGTGCCTGAAGCATCGAGCATTCGCTTCGAGGCGGATTCACTCGGGCATGTCCCGGTCCCGGC
TTCGGCCTATTATGGGGCTCAGACGGCGCGCGCGATCGCAAATTTTCAGATCTCCGGCATTCCGATCGGCCACTACCCCG
ACCTTATCCGATCTCTCGCCTATGTTAAGAAGGCAGCAGCCAGCGCCAACTTTATCCTTGGGGACCTGTCGGAAGACAAA
AAGGACGCGATCCTCCGCGCGTGCGACGAGGTTGCCGAAGGGTTGTTTGCCGATCAGTTCGTCCTCGACGTCTTCCAGGG
CGGCGCCGGCACATCCACCAACATGAATATGAATGAGGTGATCGCAAATCGCGCGCTCGAGCACCTTGGTCTGCCGCGCG
GGCGATTTGACGTCATTCATCCAAACAACGATGTCAATTTGTCTCAGTCGACCAATGACGTTTATCCAACCGCGATCCGG
TTGTCCCTCGTCCTTTCCACACGGAAATTGCTAGACGCGCTGGAATACCTTTCGGCCTCGTTCGACCGCAAGGCAGGGGA
GTTCGGCAGCATCGTGAAGCTCGGCAGGACCCAACTTCAAGATGCCGTCCCTATGACGCTCGGCCAAGAGTTCACCGCCT
TTGCAGTGACCTTACGGGAAGATATGTCACGGCTGACTGAGGCGGTTGGCCTGTTTCACGAGGTCAACCTCGGCGGTACG
GCAATCGGCACGGGGATCACAGCCGACCCCCGCTACGCCTCAACGGCGATTAGTGAGCTGGCGACGGTCTCCGGTGTGCC
TTTGCGGCCGGCAAGCGACTTTGTCGAAGCGTGCTGGGATACTGGCGCCTTCGTCCTGTTTTCCGGCACCCTGAAGCGAA
CTGCTGTCAAGATATCGAAAATTTGCAATGACCTACGTCTCCTGTCGAGCGGGCCTCGCGGCGGGTTCGGGGAAATCACG
CTGCCCCCTATGCAGCCTGGGTCGTCGATCATGCCTGGCAAGGTTAATCCCGTCATTCCCGAAATGGTCAACCAAGTTGC
TTTTCAGGTCATCGGCTCTGATCTCGTCGTCACCATGGCTGCAGAAGGCGGGCAACTGCAGTTGAATGCCTTCGAACCTG
TCATCGTCTTCAATCTACTTCAGTCGATATCGTTGATGACGAGTGCTTTCCGCACATTGGCCGACAGATGCGTCGCCGGC
ATTGTCGCCAATGAGGAGACTTGCCGGCAGAACCTTGAAGCGGGTACGGCGCTTGCGACCGCATTAACGTCGCTCATCGG
TTATGAGAAGGCGGCGGAACTCGCAAAGCAGATCCTCTCGACCGGCAGGACAATGCGAGAATTGCTGGAGGAGGACAGCA
GCCTCTCTTCGAACCTTATCGAACAGGCTCTCGAGGTGAAGTTGTTGACGCAGCCTATCAGGCTGCGGCGGGCTTGA

Upstream 100 bases:

>100_bases
GCGTTACTTGCGTCATACTGCTCATCGCTTATCACCGCAGCGAAATTTGATCGAGAAGATTGCTGAATTATTCTTCATCG
ACAACCATTGGGGATGGAAG

Downstream 100 bases:

>100_bases
GGTGTTAGTCGGAGCGGATGATCCGATAGCCCGCCCGCTCGAGCGCATCGCCTTCGCTGGAGCCGAGCGTCTCGTCGATG
ACCACTTGATCCACATCCCT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MEDAALVPEASSIRFEADSLGHVPVPASAYYGAQTARAIANFQISGIPIGHYPDLIRSLAYVKKAAASANFILGDLSEDK
KDAILRACDEVAEGLFADQFVLDVFQGGAGTSTNMNMNEVIANRALEHLGLPRGRFDVIHPNNDVNLSQSTNDVYPTAIR
LSLVLSTRKLLDALEYLSASFDRKAGEFGSIVKLGRTQLQDAVPMTLGQEFTAFAVTLREDMSRLTEAVGLFHEVNLGGT
AIGTGITADPRYASTAISELATVSGVPLRPASDFVEACWDTGAFVLFSGTLKRTAVKISKICNDLRLLSSGPRGGFGEIT
LPPMQPGSSIMPGKVNPVIPEMVNQVAFQVIGSDLVVTMAAEGGQLQLNAFEPVIVFNLLQSISLMTSAFRTLADRCVAG
IVANEETCRQNLEAGTALATALTSLIGYEKAAELAKQILSTGRTMRELLEEDSSLSSNLIEQALEVKLLTQPIRLRRA

Sequences:

>Translated_478_residues
MEDAALVPEASSIRFEADSLGHVPVPASAYYGAQTARAIANFQISGIPIGHYPDLIRSLAYVKKAAASANFILGDLSEDK
KDAILRACDEVAEGLFADQFVLDVFQGGAGTSTNMNMNEVIANRALEHLGLPRGRFDVIHPNNDVNLSQSTNDVYPTAIR
LSLVLSTRKLLDALEYLSASFDRKAGEFGSIVKLGRTQLQDAVPMTLGQEFTAFAVTLREDMSRLTEAVGLFHEVNLGGT
AIGTGITADPRYASTAISELATVSGVPLRPASDFVEACWDTGAFVLFSGTLKRTAVKISKICNDLRLLSSGPRGGFGEIT
LPPMQPGSSIMPGKVNPVIPEMVNQVAFQVIGSDLVVTMAAEGGQLQLNAFEPVIVFNLLQSISLMTSAFRTLADRCVAG
IVANEETCRQNLEAGTALATALTSLIGYEKAAELAKQILSTGRTMRELLEEDSSLSSNLIEQALEVKLLTQPIRLRRA
>Mature_478_residues
MEDAALVPEASSIRFEADSLGHVPVPASAYYGAQTARAIANFQISGIPIGHYPDLIRSLAYVKKAAASANFILGDLSEDK
KDAILRACDEVAEGLFADQFVLDVFQGGAGTSTNMNMNEVIANRALEHLGLPRGRFDVIHPNNDVNLSQSTNDVYPTAIR
LSLVLSTRKLLDALEYLSASFDRKAGEFGSIVKLGRTQLQDAVPMTLGQEFTAFAVTLREDMSRLTEAVGLFHEVNLGGT
AIGTGITADPRYASTAISELATVSGVPLRPASDFVEACWDTGAFVLFSGTLKRTAVKISKICNDLRLLSSGPRGGFGEIT
LPPMQPGSSIMPGKVNPVIPEMVNQVAFQVIGSDLVVTMAAEGGQLQLNAFEPVIVFNLLQSISLMTSAFRTLADRCVAG
IVANEETCRQNLEAGTALATALTSLIGYEKAAELAKQILSTGRTMRELLEEDSSLSSNLIEQALEVKLLTQPIRLRRA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=471, Percent_Identity=40.7643312101911, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI87082375, Length=464, Percent_Identity=50, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=435, Percent_Identity=43.9080459770115, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17553882, Length=467, Percent_Identity=41.3276231263383, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=38.6503067484663, Blast_Score=234, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6324993, Length=438, Percent_Identity=42.0091324200913, Blast_Score=330, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24640179, Length=440, Percent_Identity=41.8181818181818, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24640177, Length=440, Percent_Identity=41.8181818181818, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24662684, Length=436, Percent_Identity=40.8256880733945, Blast_Score=321, Evalue=7e-88,
Organism=Drosophila melanogaster, GI78710009, Length=437, Percent_Identity=40.2745995423341, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24583245, Length=445, Percent_Identity=37.3033707865169, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51153; Mature: 51153

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDAALVPEASSIRFEADSLGHVPVPASAYYGAQTARAIANFQISGIPIGHYPDLIRSLA
CCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCEEECCEECCCHHHHHHHHH
YVKKAAASANFILGDLSEDKKDAILRACDEVAEGLFADQFVLDVFQGGAGTSTNMNMNEV
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
IANRALEHLGLPRGRFDVIHPNNDVNLSQSTNDVYPTAIRLSLVLSTRKLLDALEYLSAS
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FDRKAGEFGSIVKLGRTQLQDAVPMTLGQEFTAFAVTLREDMSRLTEAVGLFHEVNLGGT
HHHCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AIGTGITADPRYASTAISELATVSGVPLRPASDFVEACWDTGAFVLFSGTLKRTAVKISK
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHH
ICNDLRLLSSGPRGGFGEITLPPMQPGSSIMPGKVNPVIPEMVNQVAFQVIGSDLVVTMA
HHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
AEGGQLQLNAFEPVIVFNLLQSISLMTSAFRTLADRCVAGIVANEETCRQNLEAGTALAT
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALTSLIGYEKAAELAKQILSTGRTMRELLEEDSSLSSNLIEQALEVKLLTQPIRLRRA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDAALVPEASSIRFEADSLGHVPVPASAYYGAQTARAIANFQISGIPIGHYPDLIRSLA
CCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCEEECCEECCCHHHHHHHHH
YVKKAAASANFILGDLSEDKKDAILRACDEVAEGLFADQFVLDVFQGGAGTSTNMNMNEV
HHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
IANRALEHLGLPRGRFDVIHPNNDVNLSQSTNDVYPTAIRLSLVLSTRKLLDALEYLSAS
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FDRKAGEFGSIVKLGRTQLQDAVPMTLGQEFTAFAVTLREDMSRLTEAVGLFHEVNLGGT
HHHCCCHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AIGTGITADPRYASTAISELATVSGVPLRPASDFVEACWDTGAFVLFSGTLKRTAVKISK
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHH
ICNDLRLLSSGPRGGFGEITLPPMQPGSSIMPGKVNPVIPEMVNQVAFQVIGSDLVVTMA
HHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEE
AEGGQLQLNAFEPVIVFNLLQSISLMTSAFRTLADRCVAGIVANEETCRQNLEAGTALAT
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALTSLIGYEKAAELAKQILSTGRTMRELLEEDSSLSSNLIEQALEVKLLTQPIRLRRA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]