BacMap

Definition	Rhizobium etli CIAT 652 plasmid pC, complete sequence.
Accession	NC_010997
Length	1,091,523

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The map label for this gene is fabG [C]

Identifier: 190895098

GI number: 190895098

Start: 824754

End: 825515

Strand: Reverse

Name: fabG [C]

Synonym: RHECIAT_PC0000768

Alternate gene names: 190895098

Gene position: 825515-824754 (Counterclockwise)

Preceding gene: 190895100

Following gene: 190895097

Centisome position: 75.63

GC content: 64.17

Gene sequence:

>762_bases
ATGTTACTCAATGGGAAGACCGCGGTCATTTCGGGCGCGGCGAGCAGGCGCGGCATCGGCCGTGCGACGGCGCAGCTTTT
CGCCGAGCATGGCGCTCGCGTCGCGATCCTCGATATCGATGCCGACGCCGCTGCGGAGGCCGCCGCGGCGCTGCCGAGCG
TCAAGTCTGGCAACCATGTCGGCCTGCGCTGCGACGTCGCCGAAAAGTCGTCCTGCGCCGATGCCGCATCCAAGGTGATC
GCCTCGTTCGGTTCGGTAAACGCGCTGATCAACAATGCCGGCATCACGCAGCCGGTTACCACGCTTGATATCACCGAAGC
CGACTGGCAGCGGATCGTGGCGGTCAACATGACCGGTGTGCTCTTTCTCAGCCAGGCCTTCATCCCCTACATGCGCGACA
ACGGCGGCGGATCGATAGCCTGCATGTCCTCGGTCTCGGCGCAGCGTGGCGGCGGCATCTTCGGCGGACCTCATTACTCT
GCAGCCAAGGCAGGCGTGCTCGGCCTCGCCAAAGCGATGGCTCGCGAATTCGGTCCAGCCGGCATTCGCGTCAACTCCGT
CACGCCTGGCCTCATCCAGACGGATATCACCGGTGGCAAGCTCACCGATCAAATGCGCGCCGACATCATCAAGGGCATCC
CTCTGAGCAGGCTCGGAGAGGCCGCCGACGTCGCGAATATCTACCTGTTCCTCGCCTCGGATCTGTCCGCCTACGTCACC
GGAGCGGTGATCGACGTCAACGGCGGCATGCTGATCCACTGA

Upstream 100 bases:

>100_bases
CGTTTGTCGAACGGCGTCAACTCGATCAGTTTCGAACCCACAAGCCAACTGACTTGTCAAAGATTTGCCAGATGCTCGGG
ACGCATCGGGAGGAGATTAC

Downstream 100 bases:

>100_bases
GGCCCATCTGGAGAGACACCGACATGAGCCAGATCGGCCACAATATCAGTCTGACAGCGCGCGCCCGGCGCATCCGCCGT
CACGCGCTGCGCATGGGTGA

Product: putative short chain dehydrogenase/reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLLNGKTAVISGAASRRGIGRATAQLFAEHGARVAILDIDADAAAEAAAALPSVKSGNHVGLRCDVAEKSSCADAASKVI
ASFGSVNALINNAGITQPVTTLDITEADWQRIVAVNMTGVLFLSQAFIPYMRDNGGGSIACMSSVSAQRGGGIFGGPHYS
AAKAGVLGLAKAMAREFGPAGIRVNSVTPGLIQTDITGGKLTDQMRADIIKGIPLSRLGEAADVANIYLFLASDLSAYVT
GAVIDVNGGMLIH

Sequences:

>Translated_253_residues
MLLNGKTAVISGAASRRGIGRATAQLFAEHGARVAILDIDADAAAEAAAALPSVKSGNHVGLRCDVAEKSSCADAASKVI
ASFGSVNALINNAGITQPVTTLDITEADWQRIVAVNMTGVLFLSQAFIPYMRDNGGGSIACMSSVSAQRGGGIFGGPHYS
AAKAGVLGLAKAMAREFGPAGIRVNSVTPGLIQTDITGGKLTDQMRADIIKGIPLSRLGEAADVANIYLFLASDLSAYVT
GAVIDVNGGMLIH
>Mature_253_residues
MLLNGKTAVISGAASRRGIGRATAQLFAEHGARVAILDIDADAAAEAAAALPSVKSGNHVGLRCDVAEKSSCADAASKVI
ASFGSVNALINNAGITQPVTTLDITEADWQRIVAVNMTGVLFLSQAFIPYMRDNGGGSIACMSSVSAQRGGGIFGGPHYS
AAKAGVLGLAKAMAREFGPAGIRVNSVTPGLIQTDITGGKLTDQMRADIIKGIPLSRLGEAADVANIYLFLASDLSAYVT
GAVIDVNGGMLIH

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=258, Percent_Identity=38.3720930232558, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI40254992, Length=234, Percent_Identity=35.042735042735, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI19923817, Length=260, Percent_Identity=29.2307692307692, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=30.8, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI31542939, Length=207, Percent_Identity=32.8502415458937, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI66933014, Length=228, Percent_Identity=31.5789473684211, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI5031737, Length=251, Percent_Identity=31.8725099601594, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI126723750, Length=253, Percent_Identity=30.8300395256917, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI59889578, Length=229, Percent_Identity=31.8777292576419, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI7705925, Length=257, Percent_Identity=31.1284046692607, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4503301, Length=228, Percent_Identity=30.2631578947368, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI10190704, Length=254, Percent_Identity=30.3149606299213, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI224922801, Length=183, Percent_Identity=32.7868852459016, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=31.9796954314721, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI4504505, Length=257, Percent_Identity=28.4046692607004, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4758504, Length=263, Percent_Identity=30.0380228136882, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI40807363, Length=198, Percent_Identity=29.2929292929293, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI310128830, Length=204, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI310115468, Length=204, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25282469, Length=240, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI210032110, Length=246, Percent_Identity=23.9837398373984, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI142976729, Length=195, Percent_Identity=24.6153846153846, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI20149619, Length=205, Percent_Identity=26.3414634146341, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=27.807486631016, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=39.6825396825397, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1787905, Length=247, Percent_Identity=34.8178137651822, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=32.824427480916, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789208, Length=253, Percent_Identity=32.4110671936759, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI87082160, Length=257, Percent_Identity=32.6848249027237, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=29.8804780876494, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1786812, Length=258, Percent_Identity=31.7829457364341, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI2367175, Length=261, Percent_Identity=33.7164750957854, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=30.5882352941176, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788459, Length=249, Percent_Identity=31.3253012048193, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789057, Length=240, Percent_Identity=31.6666666666667, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787526, Length=251, Percent_Identity=25.8964143426295, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367365, Length=246, Percent_Identity=29.6747967479675, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787545, Length=269, Percent_Identity=29.368029739777, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788891, Length=244, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=42.570281124498, Blast_Score=177, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17555706, Length=255, Percent_Identity=37.6470588235294, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17560676, Length=251, Percent_Identity=28.6852589641434, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=29.4776119402985, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=29.5275590551181, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=30.0380228136882, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=31.6205533596838, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17551412, Length=264, Percent_Identity=29.5454545454545, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17560150, Length=270, Percent_Identity=29.2592592592593, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17562906, Length=270, Percent_Identity=31.1111111111111, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17568967, Length=222, Percent_Identity=30.6306306306306, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=260, Percent_Identity=30.3846153846154, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17562904, Length=264, Percent_Identity=29.9242424242424, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538480, Length=264, Percent_Identity=30.6818181818182, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=29.6153846153846, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI72000259, Length=263, Percent_Identity=28.5171102661597, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536651, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=250, Percent_Identity=29.2, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=253, Percent_Identity=28.0632411067194, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17567345, Length=208, Percent_Identity=27.8846153846154, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=260, Percent_Identity=28.8461538461538, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=29.2134831460674, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=251, Percent_Identity=29.8804780876494, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561272, Length=255, Percent_Identity=29.8039215686275, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17563726, Length=260, Percent_Identity=30.3846153846154, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531453, Length=270, Percent_Identity=29.6296296296296, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=264, Percent_Identity=31.4393939393939, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=29.3233082706767, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=258, Percent_Identity=26.3565891472868, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17564282, Length=204, Percent_Identity=28.4313725490196, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=29.1187739463602, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=26.3959390862944, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=201, Percent_Identity=27.363184079602, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=201, Percent_Identity=27.363184079602, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=262, Percent_Identity=26.3358778625954, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=261, Percent_Identity=31.4176245210728, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17558628, Length=204, Percent_Identity=27.9411764705882, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=194, Percent_Identity=33.5051546391753, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=27.6679841897233, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=34.9206349206349, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21355319, Length=251, Percent_Identity=33.4661354581673, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI23397609, Length=257, Percent_Identity=31.5175097276265, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24643142, Length=249, Percent_Identity=30.5220883534137, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI18859837, Length=196, Percent_Identity=34.6938775510204, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=29.6153846153846, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24641232, Length=235, Percent_Identity=28.0851063829787, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=250, Percent_Identity=28.8, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17737361, Length=262, Percent_Identity=29.0076335877863, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364892, Length=196, Percent_Identity=29.0816326530612, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24649181, Length=204, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28571526, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24644337, Length=236, Percent_Identity=29.6610169491525, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI221331216, Length=202, Percent_Identity=30.1980198019802, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665247, Length=202, Percent_Identity=30.1980198019802, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358495, Length=222, Percent_Identity=30.1801801801802, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221331218, Length=199, Percent_Identity=28.643216080402, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24665243, Length=201, Percent_Identity=29.3532338308458, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640492, Length=199, Percent_Identity=26.1306532663317, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24641388, Length=232, Percent_Identity=29.7413793103448, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581726, Length=182, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221510726, Length=173, Percent_Identity=28.3236994219653, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI116007236, Length=234, Percent_Identity=25.6410256410256, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21357673, Length=199, Percent_Identity=27.6381909547739, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 25755; Mature: 25755

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNGKTAVISGAASRRGIGRATAQLFAEHGARVAILDIDADAAAEAAAALPSVKSGNHV
CEECCCEEEEECCHHHCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCEE
GLRCDVAEKSSCADAASKVIASFGSVNALINNAGITQPVTTLDITEADWQRIVAVNMTGV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHCCCEEEEECHHHH
LFLSQAFIPYMRDNGGGSIACMSSVSAQRGGGIFGGPHYSAAKAGVLGLAKAMAREFGPA
HHHHHHHCCEEEECCCCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GIRVNSVTPGLIQTDITGGKLTDQMRADIIKGIPLSRLGEAADVANIYLFLASDLSAYVT
CEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHCCHHHHHHEEEEHHCCHHHHHE
GAVIDVNGGMLIH
EEEEEECCCEEEC
>Mature Secondary Structure
MLLNGKTAVISGAASRRGIGRATAQLFAEHGARVAILDIDADAAAEAAAALPSVKSGNHV
CEECCCEEEEECCHHHCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCEE
GLRCDVAEKSSCADAASKVIASFGSVNALINNAGITQPVTTLDITEADWQRIVAVNMTGV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHCCCEEEEECHHHH
LFLSQAFIPYMRDNGGGSIACMSSVSAQRGGGIFGGPHYSAAKAGVLGLAKAMAREFGPA
HHHHHHHCCEEEECCCCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
GIRVNSVTPGLIQTDITGGKLTDQMRADIIKGIPLSRLGEAADVANIYLFLASDLSAYVT
CEEECCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHCCHHHHHHEEEEHHCCHHHHHE
GAVIDVNGGMLIH
EEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]