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The map label for this gene is tktB [C]

Identifier: 190895097

GI number: 190895097

Start: 823891

End: 824730

Strand: Reverse

Name: tktB [C]

Synonym: RHECIAT_PC0000767

Alternate gene names: 190895097

Gene position: 824730-823891 (Counterclockwise)

Preceding gene: 190895098

Following gene: 190895096

Centisome position: 75.56

GC content: 61.67

Gene sequence:

>840_bases
ATGAGCCAGATCGGCCACAATATCAGTCTGACAGCGCGCGCCCGGCGCATCCGCCGTCACGCGCTGCGCATGGGTGAGGT
ACAGGGGCAGGGTTATATCGCCCAGGCCCTCGGCGTCGCCGACGTCCTTGCCGTATCCTACTTCCACGCCACGAACTACC
GGCCGGAAGATCCCGAATGGGAAGGCCGCGACAGGTTCCTGTTGTCGATCGGCCACTATGCGATCGCGCTTTACGCAGCT
CTGATCGAAGCCAAGATCATTCCCGAGGATGAGCTGGAGACCTACGGCACGGATGACAGCCGGCTGCCGATGTCCGGCAT
GGCTGCCTACACGCCCGGCATGGAAATCACCGGCGGGTCGCTCGGACACGGACTAGGCATCGCGGTCGGCATGGCCTTGG
CGCTGAAGCGGAAGAAGTCCTCTTCCTTTGTCTATAATCTGTTTTCCGACGGCGAACTCGACGAGGGTTCGACCTGGGAG
GCCGCGATGTCGGCGGGTTCCTACAGGCTCGACAACCTGATCGGCATTGTCGACGTCAATCAGATGCAAGCCGACGGCCC
GTCCATTGGCGTGCTCAATTTCGAACCGCTCGGCCCAAAATTCGAGGCCTTCGGCTGGTTTGTCCAAAGGGTGGACGGCA
ACGATATCGATGCGCTCGTAAAGGCCTTCGACGCCGCTCGTCACCACGCCGAGGCGAAGCCTCGCATCATCATATGCGAC
ACGAAAATGGCGAAGGGGGTGCCATTCCTCGAGGCGCGCGATCGAAACCACTTCCTGCGCGTCGAACCCCACGAATGGGC
CGAGGCCCTCAGGATCATCGATGCGGGAGTGGAGGCATGA

Upstream 100 bases:

>100_bases
TGTTCCTCGCCTCGGATCTGTCCGCCTACGTCACCGGAGCGGTGATCGACGTCAACGGCGGCATGCTGATCCACTGAGGC
CCATCTGGAGAGACACCGAC

Downstream 100 bases:

>100_bases
GGCGTTCCAAGTACATCCGGCCTGCCCATCTTGAAAGCGGCATCGACAAGCCCCGCCTAACGACGTCGGCGATGATCGCA
TCGATTGCCGGTCCCGATCA

Product: putative transketolase, N-terminal subunit

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSQIGHNISLTARARRIRRHALRMGEVQGQGYIAQALGVADVLAVSYFHATNYRPEDPEWEGRDRFLLSIGHYAIALYAA
LIEAKIIPEDELETYGTDDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMALALKRKKSSSFVYNLFSDGELDEGSTWE
AAMSAGSYRLDNLIGIVDVNQMQADGPSIGVLNFEPLGPKFEAFGWFVQRVDGNDIDALVKAFDAARHHAEAKPRIIICD
TKMAKGVPFLEARDRNHFLRVEPHEWAEALRIIDAGVEA

Sequences:

>Translated_279_residues
MSQIGHNISLTARARRIRRHALRMGEVQGQGYIAQALGVADVLAVSYFHATNYRPEDPEWEGRDRFLLSIGHYAIALYAA
LIEAKIIPEDELETYGTDDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMALALKRKKSSSFVYNLFSDGELDEGSTWE
AAMSAGSYRLDNLIGIVDVNQMQADGPSIGVLNFEPLGPKFEAFGWFVQRVDGNDIDALVKAFDAARHHAEAKPRIIICD
TKMAKGVPFLEARDRNHFLRVEPHEWAEALRIIDAGVEA
>Mature_278_residues
SQIGHNISLTARARRIRRHALRMGEVQGQGYIAQALGVADVLAVSYFHATNYRPEDPEWEGRDRFLLSIGHYAIALYAAL
IEAKIIPEDELETYGTDDSRLPMSGMAAYTPGMEITGGSLGHGLGIAVGMALALKRKKSSSFVYNLFSDGELDEGSTWEA
AMSAGSYRLDNLIGIVDVNQMQADGPSIGVLNFEPLGPKFEAFGWFVQRVDGNDIDALVKAFDAARHHAEAKPRIIICDT
KMAKGVPFLEARDRNHFLRVEPHEWAEALRIIDAGVEA

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=271, Percent_Identity=36.5313653136531, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI205277463, Length=253, Percent_Identity=36.7588932806324, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI4507521, Length=253, Percent_Identity=36.7588932806324, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI225637459, Length=269, Percent_Identity=29.7397769516729, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI225637461, Length=269, Percent_Identity=29.368029739777, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI225637463, Length=241, Percent_Identity=30.7053941908714, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1788808, Length=251, Percent_Identity=33.8645418326693, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI48994911, Length=242, Percent_Identity=32.6446280991736, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17539652, Length=249, Percent_Identity=34.9397590361446, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325331, Length=208, Percent_Identity=34.6153846153846, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319593, Length=243, Percent_Identity=30.8641975308642, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI45551847, Length=249, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550715, Length=249, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24666278, Length=241, Percent_Identity=31.9502074688797, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645119, Length=220, Percent_Identity=35.4545454545455, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30631; Mature: 30500

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIGHNISLTARARRIRRHALRMGEVQGQGYIAQALGVADVLAVSYFHATNYRPEDPEW
CCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGRDRFLLSIGHYAIALYAALIEAKIIPEDELETYGTDDSRLPMSGMAAYTPGMEITGGS
CCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
LGHGLGIAVGMALALKRKKSSSFVYNLFSDGELDEGSTWEAAMSAGSYRLDNLIGIVDVN
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEEHHHEEEEEECC
QMQADGPSIGVLNFEPLGPKFEAFGWFVQRVDGNDIDALVKAFDAARHHAEAKPRIIICD
HHCCCCCCEEEEECCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
TKMAKGVPFLEARDRNHFLRVEPHEWAEALRIIDAGVEA
CHHHCCCCCEEECCCCCEEEECHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQIGHNISLTARARRIRRHALRMGEVQGQGYIAQALGVADVLAVSYFHATNYRPEDPEW
CCCCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGRDRFLLSIGHYAIALYAALIEAKIIPEDELETYGTDDSRLPMSGMAAYTPGMEITGGS
CCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEECCCC
LGHGLGIAVGMALALKRKKSSSFVYNLFSDGELDEGSTWEAAMSAGSYRLDNLIGIVDVN
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEEHHHEEEEEECC
QMQADGPSIGVLNFEPLGPKFEAFGWFVQRVDGNDIDALVKAFDAARHHAEAKPRIIICD
HHCCCCCCEEEEECCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
TKMAKGVPFLEARDRNHFLRVEPHEWAEALRIIDAGVEA
CHHHCCCCCEEECCCCCEEEECHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]