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The map label for this gene is gfa [H]

Identifier: 190894917

GI number: 190894917

Start: 635270

End: 635842

Strand: Reverse

Name: gfa [H]

Synonym: RHECIAT_PC0000582

Alternate gene names: 190894917

Gene position: 635842-635270 (Counterclockwise)

Preceding gene: 190894918

Following gene: 190894916

Centisome position: 58.25

GC content: 61.26

Gene sequence:

>573_bases
ATGAGCAGCATAGCCATTCATCCGGCAGTGGATTCGGGCTTTCGAGCGACTGACGCTGCTTTCACAGGCGGGACGCTGGT
GTGCAAATGCACGAGCAATCCTGTAAAGGTCAGGATCAAGGGCGATATCGCCCACAATCACGCCTGCGGCTGCACCAAGT
GCTGGAAGCCCGAGGGGGCAGTCTTTTCGGTCGTGGCGGTCGCTCCGACCGAGAGCGTCGAAGTCCTGGAGAACGGCGAC
AAACTCGCCGTCGTTGACTCGACTGCCTTGATCCAGCGGCACGCCTGCAAGGAATGCGGCGTGCATATGTATGGGCCGGT
CGAGCGCGAACACCCGTTCCAGGGATTGTCCTTCGTCCATCCCGAGCGCTTCCAGGAAGGCGGCTGGGCAAAGCCCGGCT
TTGCGGCCTTCGTGTCGTCGATCATCGAAAGCGGTTTCGATCCCGCCAAGATGGATGCCGTCAGAGCGCAGCTGAAGGCG
TCGGGTCTGGAGCCCTATGACTGCCTCTCTCCCGGCCTGATGGATTATATCGCCACCTGGATTGCCAAGAAGAGCGGCGT
GCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
ATGGAAGACCATCTGCGCTGGCACGCGCAGAGGCTTGGAAAATGATTGCAGAGCTCGGCCGGGCATAACGCAAACGGCCA
GGGAGGGAAGGAGAGAAGCA

Downstream 100 bases:

>100_bases
ATTTTTCGGGGAGCGCTGACGCCGGCGCTCCCCGATCAAGCCGGGCAGCGGCCGATAGACGTTTCTGATGCCCCTGCGCA
GCTTTGTCCGCGGGCTTCCG

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGAVFSVVAVAPTESVEVLENGD
KLAVVDSTALIQRHACKECGVHMYGPVEREHPFQGLSFVHPERFQEGGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKA
SGLEPYDCLSPGLMDYIATWIAKKSGVLAA

Sequences:

>Translated_190_residues
MSSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGAVFSVVAVAPTESVEVLENGD
KLAVVDSTALIQRHACKECGVHMYGPVEREHPFQGLSFVHPERFQEGGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKA
SGLEPYDCLSPGLMDYIATWIAKKSGVLAA
>Mature_189_residues
SSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGAVFSVVAVAPTESVEVLENGDK
LAVVDSTALIQRHACKECGVHMYGPVEREHPFQGLSFVHPERFQEGGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKAS
GLEPYDCLSPGLMDYIATWIAKKSGVLAA

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 20193; Mature: 20062

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGA
CCCEEECCCCCCCCCCCCCEECCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
VFSVVAVAPTESVEVLENGDKLAVVDSTALIQRHACKECGVHMYGPVEREHPFQGLSFVH
EEEEEEECCCCCHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PERFQEGGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKASGLEPYDCLSPGLMDYIATW
HHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHH
IAKKSGVLAA
HHHCCCCCCC
>Mature Secondary Structure 
SSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGA
CCEEECCCCCCCCCCCCCEECCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
VFSVVAVAPTESVEVLENGDKLAVVDSTALIQRHACKECGVHMYGPVEREHPFQGLSFVH
EEEEEEECCCCCHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PERFQEGGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKASGLEPYDCLSPGLMDYIATW
HHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHH
IAKKSGVLAA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]