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The map label for this gene is gcvA [H]

Identifier: 190894902

GI number: 190894902

Start: 619884

End: 620771

Strand: Reverse

Name: gcvA [H]

Synonym: RHECIAT_PC0000567

Alternate gene names: 190894902

Gene position: 620771-619884 (Counterclockwise)

Preceding gene: 190894906

Following gene: 190894899

Centisome position: 56.87

GC content: 66.22

Gene sequence:

>888_bases
ATGCAATTTCGAAGACGGCTTCCGTCGCTGACGGCGCTGGTGACGCTGGAAGCGGTGCTGCGCAGGAAGAGCTTCACCAC
GGCGGCGAGCGAACTCGGCGTCACCCAGGCGGCCGTCAGCCGGCAGATCGCCGCCCTGGAGGAGGAGCTCGGCCAGCCGC
TCTTCCTGCGCAAGCACCGGGCGATCGAGCCGACGGCCGCCTGCGTCAGCCTCGGCGCGACGCTGGCAAAGAGCTTTGCC
GACATCGCCGAGAGCGTGGAGGCGATCCAGTCGCGCAGTCAGGACGTGGTGACGATCGGCGCGACCGTCGCCTTCTCTTC
CTTCTGGCTGCTGCCGCGGCTCGCCGAATTCCGCCGGTCCAATCCCGGCATTCTCGTGAGGGTGATATCCCAGGATAACC
CGATCACGCTGGATGACGGGGAGATCGACGTGGCGATCCGATACGGCGTGCCGCCCTTCAGCGATGGAACGGTCATTGCC
TCGCGCGGCGACGTCGTCTGCCCCGTCTGCTCTCCCGACCACCTCAGGCGACGCGGAAATGGTCCGCTCGGCTCCGCCGA
CGAATTCATCGAAACGGAGGTGATCGATCGTTCCTGGTATGGCTGGGCGCAATGGGTGTCGCTGACCGGCGGCAATATCG
AGGTCAAACCGTCGCTTCGCTTCAACCATTACACCGAAAGCATCGCGGCGGCGCGCGCGGGACAGGGGATTGCCTTGGGA
TGGCGCATGCTGATCGGCACCTTCCTGGAGGACGGAACGCTGGTGTCGGCCTCAGGAACCGAGCTTGCCGCCGAAGGCCG
CTACAACGTGATCGTGCCGATCAAGGCCAAGCGCAGCCATGCGGGCGATCTCGCCGCCGCCTGGCTGACGGCCTCGCTGC
ACGGTTGA

Upstream 100 bases:

>100_bases
ATGTGAGAGTTTCCCTAGATTGCTCTTGCAGCGCCGGGCTTTCAACGGCATAAAAAATTAGCTTCGCATAAGTTTTTGTC
ATGCGAATAGGAGTCTCGTG

Downstream 100 bases:

>100_bases
TCAGAGGCCGAGGCCGGGTGTCGCCGTACGATGGACGCCCTTCGGCACGAAGGCGAACTGATCCGCCAGGCTGAGGAAAT
CGGCCGCCAGATGCATGACC

Product: putative LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHRAIEPTAACVSLGATLAKSFA
DIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRSNPGILVRVISQDNPITLDDGEIDVAIRYGVPPFSDGTVIA
SRGDVVCPVCSPDHLRRRGNGPLGSADEFIETEVIDRSWYGWAQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
WRMLIGTFLEDGTLVSASGTELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG

Sequences:

>Translated_295_residues
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHRAIEPTAACVSLGATLAKSFA
DIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRSNPGILVRVISQDNPITLDDGEIDVAIRYGVPPFSDGTVIA
SRGDVVCPVCSPDHLRRRGNGPLGSADEFIETEVIDRSWYGWAQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
WRMLIGTFLEDGTLVSASGTELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG
>Mature_295_residues
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHRAIEPTAACVSLGATLAKSFA
DIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRSNPGILVRVISQDNPITLDDGEIDVAIRYGVPPFSDGTVIA
SRGDVVCPVCSPDHLRRRGNGPLGSADEFIETEVIDRSWYGWAQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
WRMLIGTFLEDGTLVSASGTELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=272, Percent_Identity=32.3529411764706, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788706, Length=271, Percent_Identity=27.3062730627306, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=28.4722222222222, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=25.0871080139373, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31811; Mature: 31811

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50931 HTH_LYSR ; PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEHHCC
AIEPTAACVSLGATLAKSFADIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
NPGILVRVISQDNPITLDDGEIDVAIRYGVPPFSDGTVIASRGDVVCPVCSPDHLRRRGN
CCCEEEEEECCCCCEEEECCCEEEEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHCCC
GPLGSADEFIETEVIDRSWYGWAQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
CCCCCHHHHHHHHHHCCCCCCHHHEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCHHHH
WRMLIGTFLEDGTLVSASGTELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG
HHHHHHHHHCCCEEEECCCCEEECCCCEEEEEEEECCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MQFRRRLPSLTALVTLEAVLRRKSFTTAASELGVTQAAVSRQIAALEEELGQPLFLRKHR
CCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEHHCC
AIEPTAACVSLGATLAKSFADIAESVEAIQSRSQDVVTIGATVAFSSFWLLPRLAEFRRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
NPGILVRVISQDNPITLDDGEIDVAIRYGVPPFSDGTVIASRGDVVCPVCSPDHLRRRGN
CCCEEEEEECCCCCEEEECCCEEEEEEECCCCCCCCEEEECCCCEEEECCCHHHHHHCCC
GPLGSADEFIETEVIDRSWYGWAQWVSLTGGNIEVKPSLRFNHYTESIAAARAGQGIALG
CCCCCHHHHHHHHHHCCCCCCHHHEEEECCCCEEEECCCCCCHHHHHHHHHHCCCCHHHH
WRMLIGTFLEDGTLVSASGTELAAEGRYNVIVPIKAKRSHAGDLAAAWLTASLHG
HHHHHHHHHCCCEEEECCCCEEECCCCEEEEEEEECCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]