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The map label for this gene is mdtE [H]

Identifier: 190894818

GI number: 190894818

Start: 523818

End: 524891

Strand: Reverse

Name: mdtE [H]

Synonym: RHECIAT_PC0000483

Alternate gene names: 190894818

Gene position: 524891-523818 (Counterclockwise)

Preceding gene: 190894821

Following gene: 190894817

Centisome position: 48.09

GC content: 63.97

Gene sequence:

>1074_bases
ATGAGAACCATCCTGATCGCCACAGCGATCGCATGCGCCATCGGTCTCGGATCCTGCAGCGATGAGAGCAGGCCGGCTGA
GCCGACCCCGCGGCAGGTCGGTGTCGTCGTCGCCAAGCCTGAGCCGCTGGTTCAGGGGGGCGCGATCACGGGAGAGGTGC
GGGCGCGTGTCCAAAGCGATCTGTCGTTCCGCGTCAGCGGCAAGATCGTCGAACGGCTCGTCGAGGTCGGTCAGACCGTG
AAATCCGGGCAGCTCCTCGCACGCATCGATCCGGAAGAGCAGAAGGCCGACCTGGATGTGGCGGCGGCCAATCTTCAGTC
GGCCGAGGCGCAACAGACTCAGGCGCAGCTTGCATTCGACCGCCAGCAGAGCCTGTTTCGGACGCAGGTGACGACCCGCG
CCGCGCTCGATCAGGCGCAGGAGGCGCTTCTGACGGCGCAGGCATCGACCAAGTCGGCGCAGGCACTCTTCGAAACCGCT
CAGGACACCTTGTCCTACACCGAGCTGAAAGCGGATGCCGATGGGGTGATCACCGCCCGTAACGCCGAGGTCGGGCAGGT
GGCGCAGGCCGCTCAGGTCGTCTTCACGCTTGCCCATGATGGCGATCGCGATGCCGTCTTCGAGGTCGTGGAAAGCGCAT
TCCTGCGTCCGATTGACGGCGACGGCACCGTAACTCTGTTGTCGGATCCGACGCAGAAGATCACGGCGAAGGTTCGCGAG
ATTTCGCCGACAATCGATTCCGCCACCGGCACCATCAAGGTCAAGGTGGCGATCTCGAGCGATGCTCCCGTTCCGCTGGG
CGCTCCCGTCGTTGGACGGTTCAATTACCTGGCACAGGACGTCATCCAGCTGCCCTGGTCGGCAATGACCTCGAAGGACG
GCAAGCCGGCTGTCTGGATCGCCGATCCGGCATCCTCAGCGGTTTGGGCCAGAACGATCGATGTTGCCGGCTACGAAACC
GGCAGCTTCGTCGTGAAGTCGGGCGTGTCGGCGGGAGAGGTCGTGGTGACCGACGGCACCAAGTTCCTCAGGCCCGGTGA
GATCGTGTCTTACGTCAAGGAAGCTTCCAAGTGA

Upstream 100 bases:

>100_bases
TCCCTTGTCGTGCACACCCTCTTCCCTGCGGCACAAAGTTTCATTGACTAATGCGTCAGTCAATTATATAGGAGCTGCCG
TTGAGAGCAAGAGGTCAATT

Downstream 100 bases:

>100_bases
GTATTCGTCTGAAGATTGTCGCACTTATGCTGGGCACGGCCCTGGTCTCCTCCTGCGCGCCGAAAGAAGAGAGTAAGGAC
GAAACGCCTCGGCCGGTGCT

Product: putative HlyD family protein secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRTILIATAIACAIGLGSCSDESRPAEPTPRQVGVVVAKPEPLVQGGAITGEVRARVQSDLSFRVSGKIVERLVEVGQTV
KSGQLLARIDPEEQKADLDVAAANLQSAEAQQTQAQLAFDRQQSLFRTQVTTRAALDQAQEALLTAQASTKSAQALFETA
QDTLSYTELKADADGVITARNAEVGQVAQAAQVVFTLAHDGDRDAVFEVVESAFLRPIDGDGTVTLLSDPTQKITAKVRE
ISPTIDSATGTIKVKVAISSDAPVPLGAPVVGRFNYLAQDVIQLPWSAMTSKDGKPAVWIADPASSAVWARTIDVAGYET
GSFVVKSGVSAGEVVVTDGTKFLRPGEIVSYVKEASK

Sequences:

>Translated_357_residues
MRTILIATAIACAIGLGSCSDESRPAEPTPRQVGVVVAKPEPLVQGGAITGEVRARVQSDLSFRVSGKIVERLVEVGQTV
KSGQLLARIDPEEQKADLDVAAANLQSAEAQQTQAQLAFDRQQSLFRTQVTTRAALDQAQEALLTAQASTKSAQALFETA
QDTLSYTELKADADGVITARNAEVGQVAQAAQVVFTLAHDGDRDAVFEVVESAFLRPIDGDGTVTLLSDPTQKITAKVRE
ISPTIDSATGTIKVKVAISSDAPVPLGAPVVGRFNYLAQDVIQLPWSAMTSKDGKPAVWIADPASSAVWARTIDVAGYET
GSFVVKSGVSAGEVVVTDGTKFLRPGEIVSYVKEASK
>Mature_357_residues
MRTILIATAIACAIGLGSCSDESRPAEPTPRQVGVVVAKPEPLVQGGAITGEVRARVQSDLSFRVSGKIVERLVEVGQTV
KSGQLLARIDPEEQKADLDVAAANLQSAEAQQTQAQLAFDRQQSLFRTQVTTRAALDQAQEALLTAQASTKSAQALFETA
QDTLSYTELKADADGVITARNAEVGQVAQAAQVVFTLAHDGDRDAVFEVVESAFLRPIDGDGTVTLLSDPTQKITAKVRE
ISPTIDSATGTIKVKVAISSDAPVPLGAPVVGRFNYLAQDVIQLPWSAMTSKDGKPAVWIADPASSAVWARTIDVAGYET
GSFVVKSGVSAGEVVVTDGTKFLRPGEIVSYVKEASK

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=370, Percent_Identity=26.2162162162162, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082036, Length=350, Percent_Identity=28.8571428571429, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786668, Length=362, Percent_Identity=28.7292817679558, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081796, Length=328, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37605; Mature: 37605

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTILIATAIACAIGLGSCSDESRPAEPTPRQVGVVVAKPEPLVQGGAITGEVRARVQSD
CCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHCC
LSFRVSGKIVERLVEVGQTVKSGQLLARIDPEEQKADLDVAAANLQSAEAQQTQAQLAFD
CEEEEHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RQQSLFRTQVTTRAALDQAQEALLTAQASTKSAQALFETAQDTLSYTELKADADGVITAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
NAEVGQVAQAAQVVFTLAHDGDRDAVFEVVESAFLRPIDGDGTVTLLSDPTQKITAKVRE
CCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHH
ISPTIDSATGTIKVKVAISSDAPVPLGAPVVGRFNYLAQDVIQLPWSAMTSKDGKPAVWI
CCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEE
ADPASSAVWARTIDVAGYETGSFVVKSGVSAGEVVVTDGTKFLRPGEIVSYVKEASK
ECCCCCCEEEEEEEECCCCCCCHHEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRTILIATAIACAIGLGSCSDESRPAEPTPRQVGVVVAKPEPLVQGGAITGEVRARVQSD
CCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHCC
LSFRVSGKIVERLVEVGQTVKSGQLLARIDPEEQKADLDVAAANLQSAEAQQTQAQLAFD
CEEEEHHHHHHHHHHHCCCCCCCCEEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RQQSLFRTQVTTRAALDQAQEALLTAQASTKSAQALFETAQDTLSYTELKADADGVITAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
NAEVGQVAQAAQVVFTLAHDGDRDAVFEVVESAFLRPIDGDGTVTLLSDPTQKITAKVRE
CCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHH
ISPTIDSATGTIKVKVAISSDAPVPLGAPVVGRFNYLAQDVIQLPWSAMTSKDGKPAVWI
CCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEE
ADPASSAVWARTIDVAGYETGSFVVKSGVSAGEVVVTDGTKFLRPGEIVSYVKEASK
ECCCCCCEEEEEEEECCCCCCCHHEECCCCCCEEEEECCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]