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The map label for this gene is thcR [H]

Identifier: 190894821

GI number: 190894821

Start: 526969

End: 528000

Strand: Reverse

Name: thcR [H]

Synonym: RHECIAT_PC0000486

Alternate gene names: 190894821

Gene position: 528000-526969 (Counterclockwise)

Preceding gene: 190894823

Following gene: 190894818

Centisome position: 48.37

GC content: 60.66

Gene sequence:

>1032_bases
ATGGATTCGATGAGACCTGAGAATATGGCCCACTGTCCGGAGCATGGGCGTTACGGTTTTGTGCCGACGCATGAAGGCGC
CAGCTTCGAATCGATGGTGCAGGCCTTTTCGGCAGGCTATGGGGTCTTCGGCGCGCAGCCGCTGAAAAACGAACGGAAGT
TTGCCTGGGCCGCGGACCTGAGAAGGACCGAGGCGCTCACAACACTTCATTCGGTGTATGAGAGCCCCTGGACCATCCGA
ACGCTTGACGAGAGCCCGCAACATCTGACCTTTTTCATGCCGCACACCGGATCCTTTCGTCTATCGATCGGCAGGAGAAC
GGTTGAAAGCGGCGCCGGTCACCTTCTTATGGCCAACAACCACGAGGCCGGCGATCGCGTGATCCAGGGTGTTCCGCATC
GCTCGGATGCTCTCTTCCTGGACTGGAAGGTCGTGAAACAGGCGCTTGCTTCGCTGGTGGAGACGCCATTCTCCGAGTCG
CTCGACCTTGAGCCCATTCTGGATCTCACGACACAGGCCGGCCAACTCATCGGCAATCTGGTCCAAACGATTGTACAGGG
CATGCGCGCCGGCGGTCCGCTGCTGTCTTCACCCCTTGCGGCGGCGGCGATGAGCGAAACGCTCGCTCACCTCGTGATCC
GATTCGGACGCCACCGTCTTTCCGATCGTCTCGAAAGGAAGAAAGTGTCGCTGATCGCGCCTTGGCATGTCCGACGGGCC
ATCGAGTACATGCATGCCAATATCGCGGAACCTCTTACGATGACGATGGTCGCCGACGACGTCGGCGTTTCCCTTCGCGC
GCTGCAAACGGGTTTCAAGGCCTTCCGAGGGACGTCGCCGGCAGGTTACCTCCGTACGATCCGGCTGCAGGCGGCCCGCG
AGCAGCTGCGGGATCCGACGAACCAACGATCCGTCCGCGAAATCTGCGCGATATGGGGGTTTGCTCACGCGGGGAGATTT
TCCATCATCTATCGCAGCACTTTTGGCGAAAGCCCGCGGGATACGCGGCTACGGGCGGAGCGCTTGCGCTGA

Upstream 100 bases:

>100_bases
TCTGCGGGGCAAGGAGGTCATCCGTGGTTCTCGGCCCTGCTTACCGCATCGAGATTGGTCATACCAGTCGCGATCGCGCG
TCATTTCTCAAGGGAGTTGC

Downstream 100 bases:

>100_bases
TTGGAGCCTGGAACAGGATGATCTTAGGCCCGGTTGGCCTAAATCAAATCCGGTTCTAGGTTAAAGAGTTAGAGCATGAT
GCCATCCGAAAACCGCTCAC

Product: putative AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDSMRPENMAHCPEHGRYGFVPTHEGASFESMVQAFSAGYGVFGAQPLKNERKFAWAADLRRTEALTTLHSVYESPWTIR
TLDESPQHLTFFMPHTGSFRLSIGRRTVESGAGHLLMANNHEAGDRVIQGVPHRSDALFLDWKVVKQALASLVETPFSES
LDLEPILDLTTQAGQLIGNLVQTIVQGMRAGGPLLSSPLAAAAMSETLAHLVIRFGRHRLSDRLERKKVSLIAPWHVRRA
IEYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPTNQRSVREICAIWGFAHAGRF
SIIYRSTFGESPRDTRLRAERLR

Sequences:

>Translated_343_residues
MDSMRPENMAHCPEHGRYGFVPTHEGASFESMVQAFSAGYGVFGAQPLKNERKFAWAADLRRTEALTTLHSVYESPWTIR
TLDESPQHLTFFMPHTGSFRLSIGRRTVESGAGHLLMANNHEAGDRVIQGVPHRSDALFLDWKVVKQALASLVETPFSES
LDLEPILDLTTQAGQLIGNLVQTIVQGMRAGGPLLSSPLAAAAMSETLAHLVIRFGRHRLSDRLERKKVSLIAPWHVRRA
IEYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPTNQRSVREICAIWGFAHAGRF
SIIYRSTFGESPRDTRLRAERLR
>Mature_343_residues
MDSMRPENMAHCPEHGRYGFVPTHEGASFESMVQAFSAGYGVFGAQPLKNERKFAWAADLRRTEALTTLHSVYESPWTIR
TLDESPQHLTFFMPHTGSFRLSIGRRTVESGAGHLLMANNHEAGDRVIQGVPHRSDALFLDWKVVKQALASLVETPFSES
LDLEPILDLTTQAGQLIGNLVQTIVQGMRAGGPLLSSPLAAAAMSETLAHLVIRFGRHRLSDRLERKKVSLIAPWHVRRA
IEYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPTNQRSVREICAIWGFAHAGRF
SIIYRSTFGESPRDTRLRAERLR

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=195, Percent_Identity=29.2307692307692, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 38311; Mature: 38311

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSMRPENMAHCPEHGRYGFVPTHEGASFESMVQAFSAGYGVFGAQPLKNERKFAWAADL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
RRTEALTTLHSVYESPWTIRTLDESPQHLTFFMPHTGSFRLSIGRRTVESGAGHLLMANN
HHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCEEEEECCHHHHCCCCEEEEECC
HEAGDRVIQGVPHRSDALFLDWKVVKQALASLVETPFSESLDLEPILDLTTQAGQLIGNL
CCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
VQTIVQGMRAGGPLLSSPLAAAAMSETLAHLVIRFGRHRLSDRLERKKVSLIAPWHVRRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHH
IEYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPT
HHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
NQRSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRLRAERLR
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MDSMRPENMAHCPEHGRYGFVPTHEGASFESMVQAFSAGYGVFGAQPLKNERKFAWAADL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHH
RRTEALTTLHSVYESPWTIRTLDESPQHLTFFMPHTGSFRLSIGRRTVESGAGHLLMANN
HHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCEEEEECCHHHHCCCCEEEEECC
HEAGDRVIQGVPHRSDALFLDWKVVKQALASLVETPFSESLDLEPILDLTTQAGQLIGNL
CCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
VQTIVQGMRAGGPLLSSPLAAAAMSETLAHLVIRFGRHRLSDRLERKKVSLIAPWHVRRA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHH
IEYMHANIAEPLTMTMVADDVGVSLRALQTGFKAFRGTSPAGYLRTIRLQAAREQLRDPT
HHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
NQRSVREICAIWGFAHAGRFSIIYRSTFGESPRDTRLRAERLR
CHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]