Click here to switch to the map view.

The map label for this gene is nodL [H]

Identifier: 190894603

GI number: 190894603

Start: 282685

End: 283191

Strand: Reverse

Name: nodL [H]

Synonym: RHECIAT_PC0000266

Alternate gene names: 190894603

Gene position: 283191-282685 (Counterclockwise)

Preceding gene: 190894605

Following gene: 190894602

Centisome position: 25.94

GC content: 59.17

Gene sequence:

>507_bases
ATGATTGCCTCGAACGTCAAGCTGGACGACAGCTGCGTTATTCACCATCGAGATCTTGTGAATCTGTACGGCTGCACGAT
TGGCGCGGGAACGCGCATCGGCACCTTCGTTGAAATCCAGAAAAACGTCATCGTCGGCAAAGACTGCAAAATCTCCAGCC
ACTCCTTCCTCTGCGAAGGCGTGACGCTCGAGGACGGCGTTTTTATCGGCCACGGGGTGATGTTCACCAATGACACTTAC
CCGCGCGCCGTCAACGCGGACGGCAGTCTGCAGACGGAGGCCGACTGGGTCGTCATTCCCACACTGGTCAAGCGCCATGC
ATCGATCGGCAGCAACGCCACCATTCTTCCCGGCGTGACAATCGGAGAGGCCGCCCAGGTCGGCGCCGGCGCTGTCGTGA
CCAAGGATGTGCCTGACGGCGCCATTGTTGCCGGTGTCCCGGCCAGGATCACCGGTCACGTTCATGACCGGTCAGTAAAC
ATGCAAGCGTTTGGAGGAATGCGATGA

Upstream 100 bases:

>100_bases
AGAGAAATATTTGCTCTGTTGAAATGAAATCTTGCGCATTCACCCGATTGTGACCGGTTTCAGCCAGATCAGGGCCTGTT
TGAGTAGGGGACGAGACGGG

Downstream 100 bases:

>100_bases
TCGGCATTGCTGTTGTTGGATATGGATATTGGGGTCCGAACCTGGTCCGCAATATTTCGGAGGCGGGCGGCGCACAGCTC
ATTTCAGTCTGCGATCTCAA

Product: putative acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIASNVKLDDSCVIHHRDLVNLYGCTIGAGTRIGTFVEIQKNVIVGKDCKISSHSFLCEGVTLEDGVFIGHGVMFTNDTY
PRAVNADGSLQTEADWVVIPTLVKRHASIGSNATILPGVTIGEAAQVGAGAVVTKDVPDGAIVAGVPARITGHVHDRSVN
MQAFGGMR

Sequences:

>Translated_168_residues
MIASNVKLDDSCVIHHRDLVNLYGCTIGAGTRIGTFVEIQKNVIVGKDCKISSHSFLCEGVTLEDGVFIGHGVMFTNDTY
PRAVNADGSLQTEADWVVIPTLVKRHASIGSNATILPGVTIGEAAQVGAGAVVTKDVPDGAIVAGVPARITGHVHDRSVN
MQAFGGMR
>Mature_168_residues
MIASNVKLDDSCVIHHRDLVNLYGCTIGAGTRIGTFVEIQKNVIVGKDCKISSHSFLCEGVTLEDGVFIGHGVMFTNDTY
PRAVNADGSLQTEADWVVIPTLVKRHASIGSNATILPGVTIGEAAQVGAGAVVTKDVPDGAIVAGVPARITGHVHDRSVN
MQAFGGMR

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=97, Percent_Identity=35.0515463917526, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 17609; Mature: 17609

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASNVKLDDSCVIHHRDLVNLYGCTIGAGTRIGTFVEIQKNVIVGKDCKISSHSFLCEG
CCCCCCCCCCEEEEEECCEEEEEEEEECCCCCCEEEEEEECCEEECCCCCCCCCCEEEEC
VTLEDGVFIGHGVMFTNDTYPRAVNADGSLQTEADWVVIPTLVKRHASIGSNATILPGVT
CEECCCEEEECCEEEECCCCCEEECCCCCCCCCCCEEEEHHHHHHHHCCCCCCEEECCCE
IGEAAQVGAGAVVTKDVPDGAIVAGVPARITGHVHDRSVNMQAFGGMR
ECCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEECCEECCCCCCCCC
>Mature Secondary Structure
MIASNVKLDDSCVIHHRDLVNLYGCTIGAGTRIGTFVEIQKNVIVGKDCKISSHSFLCEG
CCCCCCCCCCEEEEEECCEEEEEEEEECCCCCCEEEEEEECCEEECCCCCCCCCCEEEEC
VTLEDGVFIGHGVMFTNDTYPRAVNADGSLQTEADWVVIPTLVKRHASIGSNATILPGVT
CEECCCEEEECCEEEECCCCCEEECCCCCCCCCCCEEEEHHHHHHHHCCCCCCEEECCCE
IGEAAQVGAGAVVTKDVPDGAIVAGVPARITGHVHDRSVNMQAFGGMR
ECCCCCCCCCEEEEECCCCCEEEECCCCEEEEEEECCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3132583; 2562395; 2615659 [H]