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The map label for this gene is mdeAc [H]

Identifier: 190894364

GI number: 190894364

Start: 22109

End: 23392

Strand: Reverse

Name: mdeAc [H]

Synonym: RHECIAT_PC0000024

Alternate gene names: 190894364

Gene position: 23392-22109 (Counterclockwise)

Preceding gene: 190894365

Following gene: 190894360

Centisome position: 2.14

GC content: 64.56

Gene sequence:

>1284_bases
ATGACCGCCCCGCATCCTTCCAAAACCCACATCGGCAATCACGCTCTGCATCCCGAAACGCAGATGCTGAACTACGGCTA
CGATCCCGAGCTCTCGGAAGGGGCGGTCAAGCCGCCGGTGTTCCTCACCTCCACCTTCGTCTTCAATTCGGCCGAGGACG
GGCGCGATTTCTTCGACTATGTCTCCGGCCGGCGCGAGCCGCCGGCGGGCAAGGGCGCCGGCCTCGTCTATTCACGCTTC
AACCATCCCAACAGCGAGATCGTCGAGGACAGGCTTGCGGTCTATGAGAAGACGGAAAGCGGGGCGCTGTTTTCCTCGGG
CATGGCGGCGATCGCCACGACCTTGTTCGCCTTCGTCCGGCCGGGTGATGCGATCCTGCATTCGCAGCCGCTCTACGGCG
GCACGGAAACCCTGCTGGCGAAGACCTTCCTCAATTTCGGCGTCGCCGCCGTCGGCTTTGCCGATGGCGTCAGCGAAGGC
TCGGTGCAGAAGGCGGCCGACGAGGCGGTCGCCAAGGGCCGCGTCTCCGTCATCCTGATCGAGACACCTGCCAATCCGAC
CAACAGCCTGGTCGACGTCGCGATGATCCGGCGTGTGGCCGATGCGATCGGCGCAAAGCAGGGGCACACGCCCATCATCG
TCTGCGACAACACCCTGCTCGGGCCGGTGTTCCAGCGGCCGATCGAGCATGGCGCCGATATCTCGCTCTATTCGCTGACC
AAATATGTCGGCGGCCATTCCGATCTCATCGCCGGCGCCGTTCTCGGCCGCAAGGCCGTGATCAAGCAGGTCAAGGCGCT
GCGCGGGGCGATCGGCACGCAGCTCGATCCACATTCCTGCTGGATGCTCGGCCGGTCGCTCGAGACGCTGCAGCTGCGCA
TGGAGCGGGCAAACAGCAATGCGCGGGCCGTCGCCGATTTCCTGCGCGATCACCCGAAGGTCGAGAAGGTTCATTACCTG
CCTTACCACGATCCGGAATCGCCATCGGGCCGCACCTTCGCCGCCCAATGCAGCGGCGCCGGCTCGACCTTCTCCTTCGA
TATCCGCGGCGGCCAGCCGGCCTCGTTCAAATTCCTGAACGCGCTGCAGGTCTTCAAGCTCGCCGTCAGCCTGGGCGGCA
CGGAATCGCTTGCGAGCCACCCTGCCACGATGACGCATTCCGGCGTGCCCGCCGATGTAAGGGAGCGGATCGGCGTGTTG
GAATCGACGATCCGCCTGTCGATCGGCATCGAGCATCCCGATGACCTGATCGCCGATCTGGAACTGGCGCTGCAATCCGC
TTGA

Upstream 100 bases:

>100_bases
AGACCGTAAACTGACCGTCAAGCAGCATTGTCGATGACTGCTCCCCGGCTGTGATTTTGATGTACCTTATCTCTCGGCAG
GCTCGATAGAGGAGACGATC

Downstream 100 bases:

>100_bases
CGGCGCAGGCAGGAAACGATGCCCGACGGCGCCGGGCATCGAGGGCTGCCTACTTGTCGTCGGTCGTCAGTTCGGTCTCG
TCGGAGTTCACCACGAAGAC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDYVSGRREPPAGKGAGLVYSRF
NHPNSEIVEDRLAVYEKTESGALFSSGMAAIATTLFAFVRPGDAILHSQPLYGGTETLLAKTFLNFGVAAVGFADGVSEG
SVQKAADEAVAKGRVSVILIETPANPTNSLVDVAMIRRVADAIGAKQGHTPIIVCDNTLLGPVFQRPIEHGADISLYSLT
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYL
PYHDPESPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLNALQVFKLAVSLGGTESLASHPATMTHSGVPADVRERIGVL
ESTIRLSIGIEHPDDLIADLELALQSA

Sequences:

>Translated_427_residues
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDYVSGRREPPAGKGAGLVYSRF
NHPNSEIVEDRLAVYEKTESGALFSSGMAAIATTLFAFVRPGDAILHSQPLYGGTETLLAKTFLNFGVAAVGFADGVSEG
SVQKAADEAVAKGRVSVILIETPANPTNSLVDVAMIRRVADAIGAKQGHTPIIVCDNTLLGPVFQRPIEHGADISLYSLT
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYL
PYHDPESPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLNALQVFKLAVSLGGTESLASHPATMTHSGVPADVRERIGVL
ESTIRLSIGIEHPDDLIADLELALQSA
>Mature_426_residues
TAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDYVSGRREPPAGKGAGLVYSRFN
HPNSEIVEDRLAVYEKTESGALFSSGMAAIATTLFAFVRPGDAILHSQPLYGGTETLLAKTFLNFGVAAVGFADGVSEGS
VQKAADEAVAKGRVSVILIETPANPTNSLVDVAMIRRVADAIGAKQGHTPIIVCDNTLLGPVFQRPIEHGADISLYSLTK
YVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSNARAVADFLRDHPKVEKVHYLP
YHDPESPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLNALQVFKLAVSLGGTESLASHPATMTHSGVPADVRERIGVLE
STIRLSIGIEHPDDLIADLELALQSA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=400, Percent_Identity=34.5, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI299473759, Length=400, Percent_Identity=31.75, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI25453487, Length=400, Percent_Identity=30.75, Blast_Score=168, Evalue=7e-42,
Organism=Escherichia coli, GI1790375, Length=403, Percent_Identity=34.9875930521092, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1789383, Length=412, Percent_Identity=25.9708737864078, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=34.1523341523341, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=33.1695331695332, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=32.2966507177034, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319307, Length=403, Percent_Identity=34.9875930521092, Blast_Score=199, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=29.1954022988506, Blast_Score=133, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=407, Percent_Identity=29.2383292383292, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321254, Length=430, Percent_Identity=26.046511627907, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=25.8426966292135, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922554, Length=413, Percent_Identity=36.5617433414044, Blast_Score=235, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45624; Mature: 45493

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDY
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHH
VSGRREPPAGKGAGLVYSRFNHPNSEIVEDRLAVYEKTESGALFSSGMAAIATTLFAFVR
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHC
PGDAILHSQPLYGGTETLLAKTFLNFGVAAVGFADGVSEGSVQKAADEAVAKGRVSVILI
CCCHHEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
ETPANPTNSLVDVAMIRRVADAIGAKQGHTPIIVCDNTLLGPVFQRPIEHGADISLYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
ARAVADFLRDHPKVEKVHYLPYHDPESPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHH
ALQVFKLAVSLGGTESLASHPATMTHSGVPADVRERIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHH
ELALQSA
HHHHHCC
>Mature Secondary Structure 
TAPHPSKTHIGNHALHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFNSAEDGRDFFDY
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHH
VSGRREPPAGKGAGLVYSRFNHPNSEIVEDRLAVYEKTESGALFSSGMAAIATTLFAFVR
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHC
PGDAILHSQPLYGGTETLLAKTFLNFGVAAVGFADGVSEGSVQKAADEAVAKGRVSVILI
CCCHHEECCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEE
ETPANPTNSLVDVAMIRRVADAIGAKQGHTPIIVCDNTLLGPVFQRPIEHGADISLYSLT
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEHHHHH
KYVGGHSDLIAGAVLGRKAVIKQVKALRGAIGTQLDPHSCWMLGRSLETLQLRMERANSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCC
ARAVADFLRDHPKVEKVHYLPYHDPESPSGRTFAAQCSGAGSTFSFDIRGGQPASFKFLN
HHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHH
ALQVFKLAVSLGGTESLASHPATMTHSGVPADVRERIGVLESTIRLSIGIEHPDDLIADL
HHHHHHHHHHCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHH
ELALQSA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]