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The map label for this gene is folC [H]

Identifier: 190571342

GI number: 190571342

Start: 1013369

End: 1014679

Strand: Direct

Name: folC [H]

Synonym: WPa_0947

Alternate gene names: 190571342

Gene position: 1013369-1014679 (Clockwise)

Preceding gene: 190571341

Following gene: 190571345

Centisome position: 68.36

GC content: 37.22

Gene sequence:

>1311_bases
ATGATATACATGCCTCATTGGCCTAAGCCGATTGGCAGTAGGCCAAAGGATTTTTCTCTTGATCGCATAAAAAGTTTTCT
AAATAAACTGGGTAATCCCGAGAAAAAAATACCGCCAATAATTCACATAGCTGGCACTAATGGCAAAGGATCAACACTAT
CCTTTATAAGATATATAATGCAAGCAGCAGGGTATAAAGTTCATACGTACACTTCTCCACATTTAGTGAATTTTAATGAG
AGAATAGTTGTTGCAGGCAATGATATCGATGATAGTGAATTACATAACTCACTGGAAGAATGCCGTATAGCAGTCGCAGA
ACAACCCATCACTCTATTTGAAGCCGCAACGACTGCAGCCTTCTTAGCTTTTTCTCGTCATAAAGCTGATATTACTTTAG
TTGAAGTGGGTCTGGGTGGAAGGCTTGATGCAACAAATGTTATAGATAATCCGATTCTAACGATCATCACTTCCATTGCA
CTTGACCATACAGAATATCTTGGTCCTACCGTAGAAACCATAGCAGGTGAAAAGGCTGGAATAATGAAACCTAATGTTCC
TTGCGTAATAGCACCACAAGAAAAATCTATAATGAACACGCTAGAACAACACGCAATAAACAAAAAATCTCCCTTATATA
GAGGAGGATTTGAGTGGAATTGCGAAAAACAGAATAACAGGATGGTCTTTCAATCGACTATTCAATCAATAGAATTTTCT
TTGCCATCTTTAAAAGGAGATCATCAAATAATCAATTCAGGAAACGCAATTGCAGCATGTAGCATTTTGAGTGGGAAGTA
TGGATTTAATATTGGAGAAGAGGACATTGCTTCAGGTTTACAGAGCACTTATTGGCCTGCGCGGCTAGAGTCTATAAAAG
AAGGTAATTTAATTTCTTTATTACCAAAAGATTGGCAATTATTTCTAGATGGCGCGCACAACAATGATGGTGCCAGAGTG
TTAGCTAAGTGGGTAAGAGACAATTTTGCTGAAGGTATCTATATGATCTTTGGTGTTACTCGTAACAGAAACGTCGCAGA
GTTCTTAGAGCACTTAAAGCCATACATCAAACTACTATGCGCCGTTTGTGTTAAATCTGAACCTAAAGCAACAAGTACAG
ATTTAATTAGAGAAGGAGCTCATAATATAGGAATCAATGCTATCGAATGTGAATCAATCAGAGATGCTATATCAAATCAC
ATACTAAAAGCTTCTATACAAAACGTCAAAACCATACTAATCTGTGGCTCGCTATTTTTAGCCAGAGATTTGAGTATGGA
GAATAGCTATCAATTAATCCAAGAACAATAA

Upstream 100 bases:

>100_bases
GGATAAGTATTGCTATTAATCAACGTTCTCAATTTAGTGAAAAAATCGACACGCACGGCATGGCAAGTGTAATAGATGAA
TTAAAACGTAACAATAATTT

Downstream 100 bases:

>100_bases
ATGCACTACACAACTTGCTCTCTTCCATGAGCGTGAACACTATCGGGATTTACAATGCTGCTTGGCTTTTCGGTTAATGC
TCCTATAGGTACATTTTGTT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MIYMPHWPKPIGSRPKDFSLDRIKSFLNKLGNPEKKIPPIIHIAGTNGKGSTLSFIRYIMQAAGYKVHTYTSPHLVNFNE
RIVVAGNDIDDSELHNSLEECRIAVAEQPITLFEAATTAAFLAFSRHKADITLVEVGLGGRLDATNVIDNPILTIITSIA
LDHTEYLGPTVETIAGEKAGIMKPNVPCVIAPQEKSIMNTLEQHAINKKSPLYRGGFEWNCEKQNNRMVFQSTIQSIEFS
LPSLKGDHQIINSGNAIAACSILSGKYGFNIGEEDIASGLQSTYWPARLESIKEGNLISLLPKDWQLFLDGAHNNDGARV
LAKWVRDNFAEGIYMIFGVTRNRNVAEFLEHLKPYIKLLCAVCVKSEPKATSTDLIREGAHNIGINAIECESIRDAISNH
ILKASIQNVKTILICGSLFLARDLSMENSYQLIQEQ

Sequences:

>Translated_436_residues
MIYMPHWPKPIGSRPKDFSLDRIKSFLNKLGNPEKKIPPIIHIAGTNGKGSTLSFIRYIMQAAGYKVHTYTSPHLVNFNE
RIVVAGNDIDDSELHNSLEECRIAVAEQPITLFEAATTAAFLAFSRHKADITLVEVGLGGRLDATNVIDNPILTIITSIA
LDHTEYLGPTVETIAGEKAGIMKPNVPCVIAPQEKSIMNTLEQHAINKKSPLYRGGFEWNCEKQNNRMVFQSTIQSIEFS
LPSLKGDHQIINSGNAIAACSILSGKYGFNIGEEDIASGLQSTYWPARLESIKEGNLISLLPKDWQLFLDGAHNNDGARV
LAKWVRDNFAEGIYMIFGVTRNRNVAEFLEHLKPYIKLLCAVCVKSEPKATSTDLIREGAHNIGINAIECESIRDAISNH
ILKASIQNVKTILICGSLFLARDLSMENSYQLIQEQ
>Mature_436_residues
MIYMPHWPKPIGSRPKDFSLDRIKSFLNKLGNPEKKIPPIIHIAGTNGKGSTLSFIRYIMQAAGYKVHTYTSPHLVNFNE
RIVVAGNDIDDSELHNSLEECRIAVAEQPITLFEAATTAAFLAFSRHKADITLVEVGLGGRLDATNVIDNPILTIITSIA
LDHTEYLGPTVETIAGEKAGIMKPNVPCVIAPQEKSIMNTLEQHAINKKSPLYRGGFEWNCEKQNNRMVFQSTIQSIEFS
LPSLKGDHQIINSGNAIAACSILSGKYGFNIGEEDIASGLQSTYWPARLESIKEGNLISLLPKDWQLFLDGAHNNDGARV
LAKWVRDNFAEGIYMIFGVTRNRNVAEFLEHLKPYIKLLCAVCVKSEPKATSTDLIREGAHNIGINAIECESIRDAISNH
ILKASIQNVKTILICGSLFLARDLSMENSYQLIQEQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=357, Percent_Identity=29.4117647058824, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI66932990, Length=357, Percent_Identity=29.4117647058824, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1788654, Length=408, Percent_Identity=31.1274509803922, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17553148, Length=341, Percent_Identity=25.8064516129032, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17553150, Length=341, Percent_Identity=25.8064516129032, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71984923, Length=341, Percent_Identity=25.8064516129032, Blast_Score=127, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=28.0285035629454, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=360, Percent_Identity=28.3333333333333, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322718, Length=423, Percent_Identity=23.1678486997636, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24641571, Length=381, Percent_Identity=27.5590551181102, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24581568, Length=148, Percent_Identity=32.4324324324324, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48299; Mature: 48299

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYMPHWPKPIGSRPKDFSLDRIKSFLNKLGNPEKKIPPIIHIAGTNGKGSTLSFIRYIM
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
QAAGYKVHTYTSPHLVNFNERIVVAGNDIDDSELHNSLEECRIAVAEQPITLFEAATTAA
HHCCCEEEEECCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FLAFSRHKADITLVEVGLGGRLDATNVIDNPILTIITSIALDHTEYLGPTVETIAGEKAG
HHHHHCCCCCEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
IMKPNVPCVIAPQEKSIMNTLEQHAINKKSPLYRGGFEWNCEKQNNRMVFQSTIQSIEFS
CCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHC
LPSLKGDHQIINSGNAIAACSILSGKYGFNIGEEDIASGLQSTYWPARLESIKEGNLISL
CCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEE
LPKDWQLFLDGAHNNDGARVLAKWVRDNFAEGIYMIFGVTRNRNVAEFLEHLKPYIKLLC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
AVCVKSEPKATSTDLIREGAHNIGINAIECESIRDAISNHILKASIQNVKTILICGSLFL
HHHHCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDLSMENSYQLIQEQ
HHHCCCCCCHHHHHCC
>Mature Secondary Structure
MIYMPHWPKPIGSRPKDFSLDRIKSFLNKLGNPEKKIPPIIHIAGTNGKGSTLSFIRYIM
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
QAAGYKVHTYTSPHLVNFNERIVVAGNDIDDSELHNSLEECRIAVAEQPITLFEAATTAA
HHCCCEEEEECCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FLAFSRHKADITLVEVGLGGRLDATNVIDNPILTIITSIALDHTEYLGPTVETIAGEKAG
HHHHHCCCCCEEEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
IMKPNVPCVIAPQEKSIMNTLEQHAINKKSPLYRGGFEWNCEKQNNRMVFQSTIQSIEFS
CCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHC
LPSLKGDHQIINSGNAIAACSILSGKYGFNIGEEDIASGLQSTYWPARLESIKEGNLISL
CCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCEEEE
LPKDWQLFLDGAHNNDGARVLAKWVRDNFAEGIYMIFGVTRNRNVAEFLEHLKPYIKLLC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
AVCVKSEPKATSTDLIREGAHNIGINAIECESIRDAISNHILKASIQNVKTILICGSLFL
HHHHCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARDLSMENSYQLIQEQ
HHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]