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The map label for this gene is pyrB

Identifier: 190571081

GI number: 190571081

Start: 743082

End: 743969

Strand: Direct

Name: pyrB

Synonym: WPa_0676

Alternate gene names: 190571081

Gene position: 743082-743969 (Clockwise)

Preceding gene: 190571080

Following gene: 190571082

Centisome position: 50.13

GC content: 33.11

Gene sequence:

>888_bases
ATGGACAAGAGAAGGAATTTATTAAATATCTCAGACCTCACAATCGGTGATGTAGAAAATATAACTAAATTAGCCAACCA
ATACCTTGAAGAAAAAGTTGAAAATAGTCATGTTCTGAAAAATAAAATAGTCATAAACCTATTCTTTGAAGATTCAACAC
GTACGCTCGCATCTTTTGAAATAGCAGCAAAGAGCCTTGGAGCAAATGTTATAACTTTACCAATAAAATCTTCTTCTATT
AACAAAGGGGAAGATCTAAAAGATATGATAAAAACACTAAATGCAATGAATCCTGACTATATGATAATCAGGCATAAAAG
TAGTGGTATTATTAATACATTGGCAAAGCATGTCAATTGCTCGCTAATCAACGCAGGCGATGGAAGCAGTGAACACCCAA
CTCAAGCTCTTGCAGATTATCTCGTAATTATCAGTCATAAAAAGCAAATAAAAAACCTCAAAATTGTGATATGTGGAGAT
ATTTTGCACAGTAGAGTTGCAAGATCAAATATAAGATTGCTAAAAATGTTTGGAGCAAAAATAAGCTTAGTTGCACCACC
AGCTTTGATGTGTAAACATTTTTCTGAAGTAGATTCACTTCATTATTCATTAACTGAAGGTATAAAGGATGCTGATGTAA
TTATGCTCTTGAGGTTGCAGAAAGAGCGCATGAATAATAATTCTTCAGAAAAAGAATATTTTCATTTGTATGGACTTGAT
GCACAAAAACTATCGCATGCAAAACCCGATGCAATTGTTATGCACCCAGGACCAATAAATAGAGGTATCGAAATTAGCAA
TGATATAGCAGATTGTGTTATTTTACAGCAAGTAAAGTTTGGATTAGCAACGCGTAAGGCAGTACTGCACTATTTAATAA
ATGTTTAA

Upstream 100 bases:

>100_bases
ATGAATGTAAGTGGAAATAAAATTTTATAGCTTCCTTCTACTTTACTTTACAGTTGTGTAATTGAAATTAAGAAAGTAGA
ATAAGCTCAAAAAAGGAGCT

Downstream 100 bases:

>100_bases
CTTTAATTAATTTTAAGGAATTTTAGACATAATGATCCTCCTATAAAAAACGATTTAGAAAAATATAACACACCTCATGA
CAGAAATTAAGTATGCACTG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDKRRNLLNISDLTIGDVENITKLANQYLEEKVENSHVLKNKIVINLFFEDSTRTLASFEIAAKSLGANVITLPIKSSSI
NKGEDLKDMIKTLNAMNPDYMIIRHKSSGIINTLAKHVNCSLINAGDGSSEHPTQALADYLVIISHKKQIKNLKIVICGD
ILHSRVARSNIRLLKMFGAKISLVAPPALMCKHFSEVDSLHYSLTEGIKDADVIMLLRLQKERMNNNSSEKEYFHLYGLD
AQKLSHAKPDAIVMHPGPINRGIEISNDIADCVILQQVKFGLATRKAVLHYLINV

Sequences:

>Translated_295_residues
MDKRRNLLNISDLTIGDVENITKLANQYLEEKVENSHVLKNKIVINLFFEDSTRTLASFEIAAKSLGANVITLPIKSSSI
NKGEDLKDMIKTLNAMNPDYMIIRHKSSGIINTLAKHVNCSLINAGDGSSEHPTQALADYLVIISHKKQIKNLKIVICGD
ILHSRVARSNIRLLKMFGAKISLVAPPALMCKHFSEVDSLHYSLTEGIKDADVIMLLRLQKERMNNNSSEKEYFHLYGLD
AQKLSHAKPDAIVMHPGPINRGIEISNDIADCVILQQVKFGLATRKAVLHYLINV
>Mature_295_residues
MDKRRNLLNISDLTIGDVENITKLANQYLEEKVENSHVLKNKIVINLFFEDSTRTLASFEIAAKSLGANVITLPIKSSSI
NKGEDLKDMIKTLNAMNPDYMIIRHKSSGIINTLAKHVNCSLINAGDGSSEHPTQALADYLVIISHKKQIKNLKIVICGD
ILHSRVARSNIRLLKMFGAKISLVAPPALMCKHFSEVDSLHYSLTEGIKDADVIMLLRLQKERMNNNSSEKEYFHLYGLD
AQKLSHAKPDAIVMHPGPINRGIEISNDIADCVILQQVKFGLATRKAVLHYLINV

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=298, Percent_Identity=29.8657718120805, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=30.42071197411, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1786469, Length=229, Percent_Identity=29.6943231441048, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790703, Length=228, Percent_Identity=29.3859649122807, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=324, Percent_Identity=32.0987654320988, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=312, Percent_Identity=32.6923076923077, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24642586, Length=269, Percent_Identity=34.9442379182156, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_WOLPP (B3CLL6)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975439.1
- EnsemblBacteria:   EBWOLT00000001232
- GeneID:   6385662
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0676
- GeneTree:   EBGT00050000031220
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 32918; Mature: 32918

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRRNLLNISDLTIGDVENITKLANQYLEEKVENSHVLKNKIVINLFFEDSTRTLASFE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEEECCCCHHHHHH
IAAKSLGANVITLPIKSSSINKGEDLKDMIKTLNAMNPDYMIIRHKSSGIINTLAKHVNC
HHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCE
SLINAGDGSSEHPTQALADYLVIISHKKQIKNLKIVICGDILHSRVARSNIRLLKMFGAK
EEEECCCCCCCCHHHHHHHHHHHHHCHHHHCCEEEEEEHHHHHHHHHHCCHHHEEHHCCE
ISLVAPPALMCKHFSEVDSLHYSLTEGIKDADVIMLLRLQKERMNNNSSEKEYFHLYGLD
EEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
AQKLSHAKPDAIVMHPGPINRGIEISNDIADCVILQQVKFGLATRKAVLHYLINV
HHHHCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKRRNLLNISDLTIGDVENITKLANQYLEEKVENSHVLKNKIVINLFFEDSTRTLASFE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECEEEEEEEEECCCCHHHHHH
IAAKSLGANVITLPIKSSSINKGEDLKDMIKTLNAMNPDYMIIRHKSSGIINTLAKHVNC
HHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCE
SLINAGDGSSEHPTQALADYLVIISHKKQIKNLKIVICGDILHSRVARSNIRLLKMFGAK
EEEECCCCCCCCHHHHHHHHHHHHHCHHHHCCEEEEEEHHHHHHHHHHCCHHHEEHHCCE
ISLVAPPALMCKHFSEVDSLHYSLTEGIKDADVIMLLRLQKERMNNNSSEKEYFHLYGLD
EEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
AQKLSHAKPDAIVMHPGPINRGIEISNDIADCVILQQVKFGLATRKAVLHYLINV
HHHHCCCCCCEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA