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The map label for this gene is rne [H]

Identifier: 190570603

GI number: 190570603

Start: 148177

End: 149925

Strand: Reverse

Name: rne [H]

Synonym: WPa_0141

Alternate gene names: 190570603

Gene position: 149925-148177 (Counterclockwise)

Preceding gene: 190570607

Following gene: 190570602

Centisome position: 10.11

GC content: 33.62

Gene sequence:

>1749_bases
GTGGTGAGTGATGGCAAAAGGTTATTGTTGATAGAAAGTACTACCTGTTCTAACGAGGTAAGGGTTGCCCTACTAGTTAA
TGGTAGGGTTGTAGAGTTTGAACAAGAATTCAAGGAAAAAAAACAATTAAGAGGTAATATATACGTTGCTTATGTAAAGC
GTATAGAGCCTGCTTTGCAGGCCGTGTTCATTGAGTATGGAAAAAATAAGCAAGGTTTTTTGTCTTTTTCTGAGATATCT
CTAAATTACTTTAATATTCCAGAAAAAGAGAAGGAAATTTTCCCTGATGAAGACTATTCGAGCGGTGTGGACTGTTCAAA
TGATAATTGCACAGAAGAAGGCCTAGATTCTACAGCTAGTAATGAATCCAGCAGTGGTTTTGTGAGGGGGGTACCTTTAC
ATAGAAAATATAAGTTACAAGATGTGATTTCAGTAAATCAAAAGTTATTAGTTCAATTAACTAAGGAAGAACGAGGAAAT
AAAGGAGCTTCATTTACAACATACGTAACTTTGGTCGGTAGATACTGTGTTTTCATGCCCAATTCCATAAGTAGGGGTGG
AGTATCTCGCAGAATTGAAGATACCAACGTTAGAAAACAGTTAAAGGATATATTAAATTCAATAAATTTACCAAAAGGCT
CAGGTTTAATAGTAAGAACTGTTGGTGCAGGAAAAAGTAAGAAAGAAATTGAGCAAGATTACAATAATTTGACCTTATTA
TGGCAAAATATTCAGCAAAACTTCTCTTCTTTAAATGTTCCATCATTAATTTACAACGAAGCAGATGTAGTTATGAGATC
TGTACGTGACTTTTGTAGTAATGATACAGAAATTATAGTATCTGATAGAGAAGTTTGTGAAGCGGTAAGAAAATACACTA
AAAATGTGTTACAAGGAAAATTGCGTCCTAGATTATATAGAGGTTCTGTTCCAATCTTTACTTACTATCGAGTCGAAGAT
CAAATTTCTGAATTATATAGCAATAGAGTAGAATTGCCATCTGGTGGGTCTTTGGTGATAACCTTAACTGAGGCATTTGT
TTCAATAGACGTAAATTCAGGGAAAATGACAGGAGAAGATAACATAGAAGAAACAGCTTATAGAACTAATATGGAGGCAT
TATCTGAAATATCAAGGCAAGCGAATCTCAGGGGCTTATCAGGGTTAATAGTTGTTGATTTTATTGACATGTTGAAACTT
GAGTATTGCAAAAATGTTGAATTTGCTATCAAGCAGGCGTTTAAAGATGATAAAGCTAAAGTTCAATTTAGCTTTATCAA
TGACTTTGGTTTAATGGTCTTTTCAAGACAAAGAATTAAACCAAATATACAGGAAATTAATACTACAGAGTGCTTACACT
GTAAAGGTATTGGAAGAGTGAAATCAAACGAAGTGGTTGTTGCATCGATACTAAGGGATTTGCAACATATTGCTAATAAA
AATCAAAATAAGTCCTTTGATTTAATAGCACGCAGTGCAGTTATAGCGCACATTTTTAATAATAAGCGTGATATGGTTTC
TACAATCGAAAAAGAGTTCAATATTACATTAAATGTTAGTATTGATAATAGTTTAGATGCAGATACATTTATTTTAAAGC
AAGGAGACCATATTAATTTTAATGATTATAAGCCATTGCAGAATTCTGGATGTCAAATTGTGAATAGCAGGGATAAAGAA
GCTGGTAAACTACCGGGCAACTTTTGGTTAACTAAATGGCTTTCGCGCCTTTTGGGCTCTAACAACTAA

Upstream 100 bases:

>100_bases
TGAAAGTCAACCCAATAATGCAGATCGCAAAATTCTTGACAATCAATAATATATTAAGTAGTATAAGAACATTTTAGAAT
GAAAAGTAGACGGTAAATAA

Downstream 100 bases:

>100_bases
ACGGGACTTGTAATTAACGAGTTTTATACTTATATATTTTACATCAGATAATATTTAAAAGGGAGTATTCATTATGGGAA
GAGCAATAGGTATAGATCTT

Product: ribonuclease, Rne/Rng family protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MVSDGKRLLLIESTTCSNEVRVALLVNGRVVEFEQEFKEKKQLRGNIYVAYVKRIEPALQAVFIEYGKNKQGFLSFSEIS
LNYFNIPEKEKEIFPDEDYSSGVDCSNDNCTEEGLDSTASNESSSGFVRGVPLHRKYKLQDVISVNQKLLVQLTKEERGN
KGASFTTYVTLVGRYCVFMPNSISRGGVSRRIEDTNVRKQLKDILNSINLPKGSGLIVRTVGAGKSKKEIEQDYNNLTLL
WQNIQQNFSSLNVPSLIYNEADVVMRSVRDFCSNDTEIIVSDREVCEAVRKYTKNVLQGKLRPRLYRGSVPIFTYYRVED
QISELYSNRVELPSGGSLVITLTEAFVSIDVNSGKMTGEDNIEETAYRTNMEALSEISRQANLRGLSGLIVVDFIDMLKL
EYCKNVEFAIKQAFKDDKAKVQFSFINDFGLMVFSRQRIKPNIQEINTTECLHCKGIGRVKSNEVVVASILRDLQHIANK
NQNKSFDLIARSAVIAHIFNNKRDMVSTIEKEFNITLNVSIDNSLDADTFILKQGDHINFNDYKPLQNSGCQIVNSRDKE
AGKLPGNFWLTKWLSRLLGSNN

Sequences:

>Translated_582_residues
MVSDGKRLLLIESTTCSNEVRVALLVNGRVVEFEQEFKEKKQLRGNIYVAYVKRIEPALQAVFIEYGKNKQGFLSFSEIS
LNYFNIPEKEKEIFPDEDYSSGVDCSNDNCTEEGLDSTASNESSSGFVRGVPLHRKYKLQDVISVNQKLLVQLTKEERGN
KGASFTTYVTLVGRYCVFMPNSISRGGVSRRIEDTNVRKQLKDILNSINLPKGSGLIVRTVGAGKSKKEIEQDYNNLTLL
WQNIQQNFSSLNVPSLIYNEADVVMRSVRDFCSNDTEIIVSDREVCEAVRKYTKNVLQGKLRPRLYRGSVPIFTYYRVED
QISELYSNRVELPSGGSLVITLTEAFVSIDVNSGKMTGEDNIEETAYRTNMEALSEISRQANLRGLSGLIVVDFIDMLKL
EYCKNVEFAIKQAFKDDKAKVQFSFINDFGLMVFSRQRIKPNIQEINTTECLHCKGIGRVKSNEVVVASILRDLQHIANK
NQNKSFDLIARSAVIAHIFNNKRDMVSTIEKEFNITLNVSIDNSLDADTFILKQGDHINFNDYKPLQNSGCQIVNSRDKE
AGKLPGNFWLTKWLSRLLGSNN
>Mature_582_residues
MVSDGKRLLLIESTTCSNEVRVALLVNGRVVEFEQEFKEKKQLRGNIYVAYVKRIEPALQAVFIEYGKNKQGFLSFSEIS
LNYFNIPEKEKEIFPDEDYSSGVDCSNDNCTEEGLDSTASNESSSGFVRGVPLHRKYKLQDVISVNQKLLVQLTKEERGN
KGASFTTYVTLVGRYCVFMPNSISRGGVSRRIEDTNVRKQLKDILNSINLPKGSGLIVRTVGAGKSKKEIEQDYNNLTLL
WQNIQQNFSSLNVPSLIYNEADVVMRSVRDFCSNDTEIIVSDREVCEAVRKYTKNVLQGKLRPRLYRGSVPIFTYYRVED
QISELYSNRVELPSGGSLVITLTEAFVSIDVNSGKMTGEDNIEETAYRTNMEALSEISRQANLRGLSGLIVVDFIDMLKL
EYCKNVEFAIKQAFKDDKAKVQFSFINDFGLMVFSRQRIKPNIQEINTTECLHCKGIGRVKSNEVVVASILRDLQHIANK
NQNKSFDLIARSAVIAHIFNNKRDMVSTIEKEFNITLNVSIDNSLDADTFILKQGDHINFNDYKPLQNSGCQIVNSRDKE
AGKLPGNFWLTKWLSRLLGSNN

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=512, Percent_Identity=37.109375, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI87082235, Length=516, Percent_Identity=29.6511627906977, Blast_Score=206, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 65905; Mature: 65905

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDGKRLLLIESTTCSNEVRVALLVNGRVVEFEQEFKEKKQLRGNIYVAYVKRIEPALQ
CCCCCCEEEEEECCCCCCCEEEEEEECCEEEEHHHHHHHHHHHCCCEEEEEHHHHHHHHH
AVFIEYGKNKQGFLSFSEISLNYFNIPEKEKEIFPDEDYSSGVDCSNDNCTEEGLDSTAS
HHHHHHCCCCCCCEEEEHEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCC
NESSSGFVRGVPLHRKYKLQDVISVNQKLLVQLTKEERGNKGASFTTYVTLVGRYCVFMP
CCCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECC
NSISRGGVSRRIEDTNVRKQLKDILNSINLPKGSGLIVRTVGAGKSKKEIEQDYNNLTLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHH
WQNIQQNFSSLNVPSLIYNEADVVMRSVRDFCSNDTEIIVSDREVCEAVRKYTKNVLQGK
HHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCC
LRPRLYRGSVPIFTYYRVEDQISELYSNRVELPSGGSLVITLTEAFVSIDVNSGKMTGED
CCCEEEECCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCC
NIEETAYRTNMEALSEISRQANLRGLSGLIVVDFIDMLKLEYCKNVEFAIKQAFKDDKAK
CHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
VQFSFINDFGLMVFSRQRIKPNIQEINTTECLHCKGIGRVKSNEVVVASILRDLQHIANK
EEEEEECCHHHHEEEHHHCCCCHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
NQNKSFDLIARSAVIAHIFNNKRDMVSTIEKEFNITLNVSIDNSLDADTFILKQGDHINF
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEECCCCCCCEEEEECCCCCCC
NDYKPLQNSGCQIVNSRDKEAGKLPGNFWLTKWLSRLLGSNN
CCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVSDGKRLLLIESTTCSNEVRVALLVNGRVVEFEQEFKEKKQLRGNIYVAYVKRIEPALQ
CCCCCCEEEEEECCCCCCCEEEEEEECCEEEEHHHHHHHHHHHCCCEEEEEHHHHHHHHH
AVFIEYGKNKQGFLSFSEISLNYFNIPEKEKEIFPDEDYSSGVDCSNDNCTEEGLDSTAS
HHHHHHCCCCCCCEEEEHEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCCC
NESSSGFVRGVPLHRKYKLQDVISVNQKLLVQLTKEERGNKGASFTTYVTLVGRYCVFMP
CCCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECC
NSISRGGVSRRIEDTNVRKQLKDILNSINLPKGSGLIVRTVGAGKSKKEIEQDYNNLTLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHCCCHHHH
WQNIQQNFSSLNVPSLIYNEADVVMRSVRDFCSNDTEIIVSDREVCEAVRKYTKNVLQGK
HHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCC
LRPRLYRGSVPIFTYYRVEDQISELYSNRVELPSGGSLVITLTEAFVSIDVNSGKMTGED
CCCEEEECCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCC
NIEETAYRTNMEALSEISRQANLRGLSGLIVVDFIDMLKLEYCKNVEFAIKQAFKDDKAK
CHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
VQFSFINDFGLMVFSRQRIKPNIQEINTTECLHCKGIGRVKSNEVVVASILRDLQHIANK
EEEEEECCHHHHEEEHHHCCCCHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
NQNKSFDLIARSAVIAHIFNNKRDMVSTIEKEFNITLNVSIDNSLDADTFILKQGDHINF
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCEEEEEEEECCCCCCCEEEEECCCCCCC
NDYKPLQNSGCQIVNSRDKEAGKLPGNFWLTKWLSRLLGSNN
CCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]