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The map label for this gene is rnhA [H]

Identifier: 190570550

GI number: 190570550

Start: 76670

End: 77107

Strand: Reverse

Name: rnhA [H]

Synonym: WPa_0080

Alternate gene names: 190570550

Gene position: 77107-76670 (Counterclockwise)

Preceding gene: 190570551

Following gene: 190570549

Centisome position: 5.2

GC content: 33.79

Gene sequence:

>438_bases
ATGAAGAAAAAGGAAGTGACAATATACACAGATGGAGCATGCTCTGGTAACCCAGGGACAGGAGGGTGGGCAGCAATCAT
ATTATTTCAAAATCATAGAAAGAACATCTGTGGTAGAGAAGAAAATACTACAAATAATAAAATGGAGTTAACAGCAGTGA
TCAATGGACTGAAGGTATTAAAATTTCCTTGTAATATCAGTTTGTATACGGATAGCCTCTATATTAAATACGGTATAACA
GAATGGATAAATAAGTGGAAAATGAACGGCTGGAAGACAAGTAATAAGAAGTCAGTAAAAAATATAGAATTATGGAAAGA
ATTAGATAATGCTGCTTTGCAACACGAAATTAATTGGAATTGGGTGAAAGCGCATAATGGTGACAAATACAATGAGGAAG
CTGATATTCTAGCAAGAAAAGCAATAATCAATGCTTAA

Upstream 100 bases:

>100_bases
TAATAAAATTTAAAGGTGCGGCTTAAATTGCCTCTATATCTATAAAAATTGTGATTTTATAGAAGCAACGATATACTTTA
TTAATTCACTACAGTTAAAG

Downstream 100 bases:

>100_bases
AAAAATTACTATATCATTTTCTATAATTTTATTATTTATATCCTGCTTCTTTATCTTTTTTAAAGGTGAGGGACCTCTAG
AAATCAATATTAGTTATATT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKKKEVTIYTDGACSGNPGTGGWAAIILFQNHRKNICGREENTTNNKMELTAVINGLKVLKFPCNISLYTDSLYIKYGIT
EWINKWKMNGWKTSNKKSVKNIELWKELDNAALQHEINWNWVKAHNGDKYNEEADILARKAIINA

Sequences:

>Translated_145_residues
MKKKEVTIYTDGACSGNPGTGGWAAIILFQNHRKNICGREENTTNNKMELTAVINGLKVLKFPCNISLYTDSLYIKYGIT
EWINKWKMNGWKTSNKKSVKNIELWKELDNAALQHEINWNWVKAHNGDKYNEEADILARKAIINA
>Mature_145_residues
MKKKEVTIYTDGACSGNPGTGGWAAIILFQNHRKNICGREENTTNNKMELTAVINGLKVLKFPCNISLYTDSLYIKYGIT
EWINKWKMNGWKTSNKKSVKNIELWKELDNAALQHEINWNWVKAHNGDKYNEEADILARKAIINA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=141, Percent_Identity=37.5886524822695, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1786408, Length=142, Percent_Identity=50.7042253521127, Blast_Score=155, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17505482, Length=134, Percent_Identity=31.3432835820896, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=33.5714285714286, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45552509, Length=143, Percent_Identity=37.7622377622378, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137476, Length=143, Percent_Identity=37.7622377622378, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16602; Mature: 16602

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKEVTIYTDGACSGNPGTGGWAAIILFQNHRKNICGREENTTNNKMELTAVINGLKVL
CCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCEEEE
KFPCNISLYTDSLYIKYGITEWINKWKMNGWKTSNKKSVKNIELWKELDNAALQHEINWN
EECCCEEEEECCEEEEECHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHEECCCCE
WVKAHNGDKYNEEADILARKAIINA
EEEECCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKEVTIYTDGACSGNPGTGGWAAIILFQNHRKNICGREENTTNNKMELTAVINGLKVL
CCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCEEEE
KFPCNISLYTDSLYIKYGITEWINKWKMNGWKTSNKKSVKNIELWKELDNAALQHEINWN
EECCCEEEEECCEEEEECHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHEECCCCE
WVKAHNGDKYNEEADILARKAIINA
EEEECCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA