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The map label for this gene is ctaB

Identifier: 190570551

GI number: 190570551

Start: 77283

End: 78170

Strand: Reverse

Name: ctaB

Synonym: WPa_0081

Alternate gene names: 190570551

Gene position: 78170-77283 (Counterclockwise)

Preceding gene: 190570552

Following gene: 190570550

Centisome position: 5.27

GC content: 34.8

Gene sequence:

>888_bases
GTGTATATAAGTGCTTTGTTAAACGTTGAATCGACAATATTGGACTTTTGGCATTTGCTGAAGCCAAGGATAATGTACCT
TGTAGTGTTTACTGCAATTGCTGGAATGGTTGCTGCACCAGGTAGCATTCATCCTTTCCTTGCACTAATATCTCTTATGT
GCATTGCTCTTGGTTCAGGATCTGCAGGTGCAATAAATATGTGGTATGACAGAGATATAGACTTGGTTATGGAGAGGACA
AAAAATCGTCCTATACCTTCAGGTAGAGTTTTTGCAGAAAGCGCGCTTGAGTTTGGCATAACTATTGGAATATTGTCAGT
ATTTATCATGGCAATAGCAGTAAATTATATTTCTGCTGCTTTACTTGCAGTTAGCATATTATTTTACGTTTTCGTATATA
CAATTTGGCTAAAAAGGCGTACTCCGCAGAATATCGTTATCGGTGGTGCAGCAGGTGCTTTTCCTCCAATGATTGGCTGG
GCGGTTGTAACTGACTCTGTAAGTTGGGAAAGTTTTATTCTATTTTTAATAATTTTTATGTGGACTCCACCACATTTTTG
GGCTCTATCTTTAAATAGATCTGAGGACTATGTAAAAGCATCGATACCAATGTTTAATGTTATCCATGGACCGAAAAAGA
CAAGAAAACATATATTAATTTATAGTATATTGTTGGTATTAACTAGCTTACTCCCAGCACTATTTTTGAAAAAGTCATTA
TTCTACCTCAGTATGGCAATTATTGAAGGTTGCGTTTTTATTTGGTTTGCCATATCAGTTATAAGGCTTAAGAATCATAG
CTCGCAGAAAAAAATGTTTTCTTACTCAATTTCTTATCTATTTTCTCTATTTGCTAGTATTATTTTTTGTTCTATTGACT
TATTTTAA

Upstream 100 bases:

>100_bases
AAAAATGGAGATAATCCTTGGGGGGGTGATACCTTAGAGTGGACGGTATCTTCACCACCACCTTTTCATACTTTCGAAAA
GCCACCAGTGGTAAAATAAA

Downstream 100 bases:

>100_bases
CTATGAAAGAACATAAAAACTATCTTTTACTTTCTCTTCTAATAATGCTCGTTGTAGCACTTTTTTTTATTTCCATAATA
AAATTTAAAGGTGCGGCTTA

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MYISALLNVESTILDFWHLLKPRIMYLVVFTAIAGMVAAPGSIHPFLALISLMCIALGSGSAGAINMWYDRDIDLVMERT
KNRPIPSGRVFAESALEFGITIGILSVFIMAIAVNYISAALLAVSILFYVFVYTIWLKRRTPQNIVIGGAAGAFPPMIGW
AVVTDSVSWESFILFLIIFMWTPPHFWALSLNRSEDYVKASIPMFNVIHGPKKTRKHILIYSILLVLTSLLPALFLKKSL
FYLSMAIIEGCVFIWFAISVIRLKNHSSQKKMFSYSISYLFSLFASIIFCSIDLF

Sequences:

>Translated_295_residues
MYISALLNVESTILDFWHLLKPRIMYLVVFTAIAGMVAAPGSIHPFLALISLMCIALGSGSAGAINMWYDRDIDLVMERT
KNRPIPSGRVFAESALEFGITIGILSVFIMAIAVNYISAALLAVSILFYVFVYTIWLKRRTPQNIVIGGAAGAFPPMIGW
AVVTDSVSWESFILFLIIFMWTPPHFWALSLNRSEDYVKASIPMFNVIHGPKKTRKHILIYSILLVLTSLLPALFLKKSL
FYLSMAIIEGCVFIWFAISVIRLKNHSSQKKMFSYSISYLFSLFASIIFCSIDLF
>Mature_295_residues
MYISALLNVESTILDFWHLLKPRIMYLVVFTAIAGMVAAPGSIHPFLALISLMCIALGSGSAGAINMWYDRDIDLVMERT
KNRPIPSGRVFAESALEFGITIGILSVFIMAIAVNYISAALLAVSILFYVFVYTIWLKRRTPQNIVIGGAAGAFPPMIGW
AVVTDSVSWESFILFLIIFMWTPPHFWALSLNRSEDYVKASIPMFNVIHGPKKTRKHILIYSILLVLTSLLPALFLKKSL
FYLSMAIIEGCVFIWFAISVIRLKNHSSQKKMFSYSISYLFSLFASIIFCSIDLF

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=192, Percent_Identity=34.8958333333333, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1786631, Length=268, Percent_Identity=29.1044776119403, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71998235, Length=275, Percent_Identity=26.9090909090909, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325085, Length=231, Percent_Identity=34.6320346320346, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921064, Length=273, Percent_Identity=30.03663003663, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COXX_WOLPP (B3CN58)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001974909.1
- EnsemblBacteria:   EBWOLT00000000219
- GeneID:   6385360
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0081
- GeneTree:   EBGT00050000031248
- HOGENOM:   HBG656003
- OMA:   LLKPRIM
- ProtClustDB:   PRK04375
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.- [C]

Molecular weight: Translated: 33149; Mature: 33149

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13451150)-; HASH(0x12e82e78)-; HASH(0x12a710b8)-; HASH(0x12c1a384)-; HASH(0x1340f558)-; HASH(0x12b389f0)-; HASH(0x139b9dc0)-; HASH(0x12d71764)-; HASH(0x13eb3348)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYISALLNVESTILDFWHLLKPRIMYLVVFTAIAGMVAAPGSIHPFLALISLMCIALGSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
SAGAINMWYDRDIDLVMERTKNRPIPSGRVFAESALEFGITIGILSVFIMAIAVNYISAA
CCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAVSILFYVFVYTIWLKRRTPQNIVIGGAAGAFPPMIGWAVVTDSVSWESFILFLIIFM
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
WTPPHFWALSLNRSEDYVKASIPMFNVIHGPKKTRKHILIYSILLVLTSLLPALFLKKSL
HCCCCEEEEEECCCCCHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLSMAIIEGCVFIWFAISVIRLKNHSSQKKMFSYSISYLFSLFASIIFCSIDLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MYISALLNVESTILDFWHLLKPRIMYLVVFTAIAGMVAAPGSIHPFLALISLMCIALGSG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
SAGAINMWYDRDIDLVMERTKNRPIPSGRVFAESALEFGITIGILSVFIMAIAVNYISAA
CCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAVSILFYVFVYTIWLKRRTPQNIVIGGAAGAFPPMIGWAVVTDSVSWESFILFLIIFM
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
WTPPHFWALSLNRSEDYVKASIPMFNVIHGPKKTRKHILIYSILLVLTSLLPALFLKKSL
HCCCCEEEEEECCCCCHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYLSMAIIEGCVFIWFAISVIRLKNHSSQKKMFSYSISYLFSLFASIIFCSIDLF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA