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The map label for this gene is mnmG [H]

Identifier: 189499008

GI number: 189499008

Start: 9486

End: 11357

Strand: Reverse

Name: mnmG [H]

Synonym: Cphamn1_0008

Alternate gene names: 189499008

Gene position: 11357-9486 (Counterclockwise)

Preceding gene: 189499015

Following gene: 189499007

Centisome position: 0.42

GC content: 48.99

Gene sequence:

>1872_bases
ATGAACATCTACGACCTAATAGTGGCAGGAGCAGGCCATGCAGGCTGTGAGGCGGCACTTGCCGGGGCCCGAATGGGCCT
GAAGTGCCTGCTCATCACCTCTGATTTTTCTGCAATCGCCAGAATGTCCTGCAATCCGGCTATCGGCGGCGTTGCAAAAG
GCCAGATAACAAGAGAAATCGATGCTCTCGGCGGCGAAATGGGAAAAGCAATCGATGCGACCGGAATTCAGTTCAGAATG
CTCAACAGAAGCAAAGGACCCGCAATGCACTCCCCGAGAGCCCAGGCCGACCGAACGAACTACTCGGCCTACATGCGAAA
AATCATAGAACAGGAAACAAATATTGATCTGCTCCAGGACAGCGTTATCAGAATCGCGACAAATCAGGGCACCTGCAACG
GCGCCATAGTCAGCTCCGGGCGCACCATAGAAGCAAAAGCCGTCATCCTCTCCTGCGGAACGTTTCTCAACGGCCTGATT
CATATTGGAATGAATCATTACCCCGGAGGAAGAACCATTGCAGAGCCGCCAGTCCAAGGCCTTACGGAATCCCTTGCCTC
ACTTGGCTTTGAGCACGGGCGCCTGAAAACAGGTACACCCCCGAGAATAGATCAAAGAAGTGTTGATTATGCCAGGGTTT
CTGTTCAGCCGGGTGACAGGGACCCCTCGCCCTTTTCATTCTCCACAGAGAGCCTTGCCGGCAGGAAGCAGGTCGAATGC
TTTCTGACCGCCACAACCGAAAATACTCACCGCATTCTACAGGAGGGTTTCGAACGCTCACCGCTTTTCTCAGGAAAAGT
ACAGGGCGTCGGCCCACGATACTGCCCTTCTATTGAAGATAAAATTCATCGTTTCAGAGAGAAAAACAGCCACCATATCT
TTCTCGAACCTGAAGGGTTTGAAACAAACGAAATGTATGTAAACGGTTTTTCAACCTCCCTTCCTGAAGATATTCAACTC
AAAGGACTGCGTTCTATTCCCGGACTGGAAAACGTCATCATGATGCGCCCGGGCTACGCCATTGAATACGATTATTTTTA
TCCTTTTCAACTTCACCCCACACTTGAGACAAAGCGTATAAAAAACCTGTATTTTGCCGGACAGATAAACGGAACATCGG
GTTATGAAGAGGCCGCTGCGCAGGGAATTATGGCGGGGATCAATGCCGCACTGAATATCCGGCAAAAAGAGCCCTTGATC
CTTAAACGATCTGAAGCATATATCGGCGTACTGATCGATGACCTGATCACGAAAGAAACAAACGAACCGTACAGAATGTT
TACCTCTTCCGCTGAACACCGCATCATACTCCGCCAGGATAATGCGGATTTACGTCTTATGCCTTTTGGTTACGCTTGCG
GACTGCTTTCTGAAAATACCTATCGCAACCTCGAGATAAAACGACGAAACATCGCCTCTGTTAAGGAGCTTATTTCATCT
ATAAGGGTGGCATCCGACTCCATCAATGCACTGCTGGCCGAACAAGGCTCCCCTTCGATATCCGGAAGCACGAGAATAGC
AACGATACTGAAACGCCCCAAGATCACGCTTTCGTCACTAGTAAACGCTTTTCCGGAAGTAGCAGATCAAATATCCGAAG
TCACACAGGACAATACAGTACTGGAACAGGTTGAAATCGATTGCAAGTACGAAGGATATATACAACGCGAGCGGCTTATG
GCCGAAAAAATCGCGCGTCTTGATTCTCACAGGATCCCTGCATCGTTCAACTATAGTGCGCTCAAGGGTCTTTCAAATGA
AGGAAAAGAAAAATTATCAAAAGTTAAGCCCTCGACAGTAGGGCAGGCGTCAAGAATACTGGGAGTTACACCTGCTGATA
TCTCAGTCCTCATGGTGAAAATAGGCCGATAG

Upstream 100 bases:

>100_bases
ACAAGTAGATCTCACGCCAGGGCAAAAAAAACCATAACTCTTTCTATTATAATATTTTAACCGGAACCACAAATCATGTT
TCACGTGAAACACATTAGTA

Downstream 100 bases:

>100_bases
AGCGACTTAACAGAGATGTTTCACGTGAAACGTTCAATTCTCACGTTCACGTCTAACGAGATACAAAACTGATATGAGTA
ACAACCAACAGAACAACATC

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MNIYDLIVAGAGHAGCEAALAGARMGLKCLLITSDFSAIARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRM
LNRSKGPAMHSPRAQADRTNYSAYMRKIIEQETNIDLLQDSVIRIATNQGTCNGAIVSSGRTIEAKAVILSCGTFLNGLI
HIGMNHYPGGRTIAEPPVQGLTESLASLGFEHGRLKTGTPPRIDQRSVDYARVSVQPGDRDPSPFSFSTESLAGRKQVEC
FLTATTENTHRILQEGFERSPLFSGKVQGVGPRYCPSIEDKIHRFREKNSHHIFLEPEGFETNEMYVNGFSTSLPEDIQL
KGLRSIPGLENVIMMRPGYAIEYDYFYPFQLHPTLETKRIKNLYFAGQINGTSGYEEAAAQGIMAGINAALNIRQKEPLI
LKRSEAYIGVLIDDLITKETNEPYRMFTSSAEHRIILRQDNADLRLMPFGYACGLLSENTYRNLEIKRRNIASVKELISS
IRVASDSINALLAEQGSPSISGSTRIATILKRPKITLSSLVNAFPEVADQISEVTQDNTVLEQVEIDCKYEGYIQRERLM
AEKIARLDSHRIPASFNYSALKGLSNEGKEKLSKVKPSTVGQASRILGVTPADISVLMVKIGR

Sequences:

>Translated_623_residues
MNIYDLIVAGAGHAGCEAALAGARMGLKCLLITSDFSAIARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRM
LNRSKGPAMHSPRAQADRTNYSAYMRKIIEQETNIDLLQDSVIRIATNQGTCNGAIVSSGRTIEAKAVILSCGTFLNGLI
HIGMNHYPGGRTIAEPPVQGLTESLASLGFEHGRLKTGTPPRIDQRSVDYARVSVQPGDRDPSPFSFSTESLAGRKQVEC
FLTATTENTHRILQEGFERSPLFSGKVQGVGPRYCPSIEDKIHRFREKNSHHIFLEPEGFETNEMYVNGFSTSLPEDIQL
KGLRSIPGLENVIMMRPGYAIEYDYFYPFQLHPTLETKRIKNLYFAGQINGTSGYEEAAAQGIMAGINAALNIRQKEPLI
LKRSEAYIGVLIDDLITKETNEPYRMFTSSAEHRIILRQDNADLRLMPFGYACGLLSENTYRNLEIKRRNIASVKELISS
IRVASDSINALLAEQGSPSISGSTRIATILKRPKITLSSLVNAFPEVADQISEVTQDNTVLEQVEIDCKYEGYIQRERLM
AEKIARLDSHRIPASFNYSALKGLSNEGKEKLSKVKPSTVGQASRILGVTPADISVLMVKIGR
>Mature_623_residues
MNIYDLIVAGAGHAGCEAALAGARMGLKCLLITSDFSAIARMSCNPAIGGVAKGQITREIDALGGEMGKAIDATGIQFRM
LNRSKGPAMHSPRAQADRTNYSAYMRKIIEQETNIDLLQDSVIRIATNQGTCNGAIVSSGRTIEAKAVILSCGTFLNGLI
HIGMNHYPGGRTIAEPPVQGLTESLASLGFEHGRLKTGTPPRIDQRSVDYARVSVQPGDRDPSPFSFSTESLAGRKQVEC
FLTATTENTHRILQEGFERSPLFSGKVQGVGPRYCPSIEDKIHRFREKNSHHIFLEPEGFETNEMYVNGFSTSLPEDIQL
KGLRSIPGLENVIMMRPGYAIEYDYFYPFQLHPTLETKRIKNLYFAGQINGTSGYEEAAAQGIMAGINAALNIRQKEPLI
LKRSEAYIGVLIDDLITKETNEPYRMFTSSAEHRIILRQDNADLRLMPFGYACGLLSENTYRNLEIKRRNIASVKELISS
IRVASDSINALLAEQGSPSISGSTRIATILKRPKITLSSLVNAFPEVADQISEVTQDNTVLEQVEIDCKYEGYIQRERLM
AEKIARLDSHRIPASFNYSALKGLSNEGKEKLSKVKPSTVGQASRILGVTPADISVLMVKIGR

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=629, Percent_Identity=39.7456279809221, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI19882217, Length=654, Percent_Identity=38.2262996941896, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI183227703, Length=669, Percent_Identity=37.3692077727952, Blast_Score=411, Evalue=1e-114,
Organism=Escherichia coli, GI2367273, Length=626, Percent_Identity=48.5623003194888, Blast_Score=584, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17534255, Length=618, Percent_Identity=36.8932038834951, Blast_Score=410, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=43.6187399030695, Blast_Score=492, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24658174, Length=625, Percent_Identity=40.8, Blast_Score=451, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 68440; Mature: 68440

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYDLIVAGAGHAGCEAALAGARMGLKCLLITSDFSAIARMSCNPAIGGVAKGQITREI
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCCCCCHHHHHH
DALGGEMGKAIDATGIQFRMLNRSKGPAMHSPRAQADRTNYSAYMRKIIEQETNIDLLQD
HHHCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCC
SVIRIATNQGTCNGAIVSSGRTIEAKAVILSCGTFLNGLIHIGMNHYPGGRTIAEPPVQG
CEEEEEECCCCCCCEEEECCCEEEEEEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCHHH
LTESLASLGFEHGRLKTGTPPRIDQRSVDYARVSVQPGDRDPSPFSFSTESLAGRKQVEC
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCEEEE
FLTATTENTHRILQEGFERSPLFSGKVQGVGPRYCPSIEDKIHRFREKNSHHIFLEPEGF
EEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCC
ETNEMYVNGFSTSLPEDIQLKGLRSIPGLENVIMMRPGYAIEYDYFYPFQLHPTLETKRI
CCCCEEEECCCCCCCCCCEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEECCCHHHHHH
KNLYFAGQINGTSGYEEAAAQGIMAGINAALNIRQKEPLILKRSEAYIGVLIDDLITKET
HEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCEEEHHHHHHHHCCC
NEPYRMFTSSAEHRIILRQDNADLRLMPFGYACGLLSENTYRNLEIKRRNIASVKELISS
CCCHHHHCCCCCCEEEEEECCCCEEEEECHHECCCCCCCCCCCEEEEHHHHHHHHHHHHH
IRVASDSINALLAEQGSPSISGSTRIATILKRPKITLSSLVNAFPEVADQISEVTQDNTV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
LEQVEIDCKYEGYIQRERLMAEKIARLDSHRIPASFNYSALKGLSNEGKEKLSKVKPSTV
EEHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCC
GQASRILGVTPADISVLMVKIGR
CCHHHEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MNIYDLIVAGAGHAGCEAALAGARMGLKCLLITSDFSAIARMSCNPAIGGVAKGQITREI
CCCEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHCCCCCCCCCCCCCHHHHHH
DALGGEMGKAIDATGIQFRMLNRSKGPAMHSPRAQADRTNYSAYMRKIIEQETNIDLLQD
HHHCHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCC
SVIRIATNQGTCNGAIVSSGRTIEAKAVILSCGTFLNGLIHIGMNHYPGGRTIAEPPVQG
CEEEEEECCCCCCCEEEECCCEEEEEEEEEEHHHHHHHHHEECCCCCCCCCCCCCCCHHH
LTESLASLGFEHGRLKTGTPPRIDQRSVDYARVSVQPGDRDPSPFSFSTESLAGRKQVEC
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCCEEEE
FLTATTENTHRILQEGFERSPLFSGKVQGVGPRYCPSIEDKIHRFREKNSHHIFLEPEGF
EEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCC
ETNEMYVNGFSTSLPEDIQLKGLRSIPGLENVIMMRPGYAIEYDYFYPFQLHPTLETKRI
CCCCEEEECCCCCCCCCCEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEECCCHHHHHH
KNLYFAGQINGTSGYEEAAAQGIMAGINAALNIRQKEPLILKRSEAYIGVLIDDLITKET
HEEEEEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCEEEHHHHHHHHCCC
NEPYRMFTSSAEHRIILRQDNADLRLMPFGYACGLLSENTYRNLEIKRRNIASVKELISS
CCCHHHHCCCCCCEEEEEECCCCEEEEECHHECCCCCCCCCCCEEEEHHHHHHHHHHHHH
IRVASDSINALLAEQGSPSISGSTRIATILKRPKITLSSLVNAFPEVADQISEVTQDNTV
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
LEQVEIDCKYEGYIQRERLMAEKIARLDSHRIPASFNYSALKGLSNEGKEKLSKVKPSTV
EEHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCC
GQASRILGVTPADISVLMVKIGR
CCHHHEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA