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The map label for this gene is gyrB [H]

Identifier: 189499015

GI number: 189499015

Start: 19036

End: 20970

Strand: Reverse

Name: gyrB [H]

Synonym: Cphamn1_0015

Alternate gene names: 189499015

Gene position: 20970-19036 (Counterclockwise)

Preceding gene: 189499016

Following gene: 189499008

Centisome position: 0.77

GC content: 48.22

Gene sequence:

>1935_bases
ATGTCAGAGATCCAGAAAAACACCTCTCCTCAAGCCTATGAGGCCACAAATATTCAGATACTTGACGGCATAGAGCACGT
TCGAAAAAGGCCCGCAATGTATATCGGCGACCTTCATTCAAGAGGGCTGCACCATCTTATTTATGAAATCGTCGACAACT
CTATTGATGAAACTCTGGCAGGATACAACGAATCTATCGAGCTTTCATTGAATCCTGACGGCTCTGTAACCGTGACGGAT
AACGGCAGGGGAATACCGGTCGACATACATCCGCAGAAGAAAAAATCAGCTCTTGAACTGGTTATGACGCTCATCGGCGC
GGGAGGTAAATTCGATAAGGGGGCGTATAAGGTTTCAGGGGGACTGCACGGTGTCGGCGCCTCTGTAGTAAACGCTCTTT
CCGAATGGTGTGAGGTTGAAGTCTACCGGGAAGGGAATATCTGGTTTCAGCGATATCACAAAGGAATCCCCCAACGCGAT
GTAGAGGTTATCGGCGAATCAACAAAAACCGGTACGAAAACCACCTTCATGCCGGATCCGGATATATTCAAAATCACGGA
GTTTCGTAAGGATATTGTTGTTGACCGCATGCGCGAACTTGCCTTTCTCAACAATATCCTGAAAATAGTCGTCAAAGACA
TTGACGGTTCCGAAGAGACGTTTCACTATGAAGGCGGCATAAAAGAATTTGTCACCTTTACCGACCATAACAGAATCAGT
CTTCTCAAGGAACCTGTGTATATCAGCGGTGAGCGGGATTCGACAGTTGTCGAAATCGCCCTGCAGTATAACGACTCGTA
TCAGGAAAATGTTTTCAGCTACGTCAACAACATCAACACCCATGAGGGGGGAACGCATGTTACCGGATTTCGCAAGGCCC
TGACAAGAACCCTCAATTCATATGCCCAGAAGAACGATCTCCTGAAAAACCTGAAACTTTCTCTCACAGGCGATGACTTC
AAAGAGGGCCTGACAGCGATCATATCGGTTAAAGTCGCCGAGCCGCAGTTCGAAGGACAGACAAAAACCAAACTGGGCAA
CTCGGAAACGCAGAGCATTGTCGAAAGCATTGTCAATGAACAACTTTCTGATTTCGCTGAAAGCAACCCCGGTGTACTGA
AAACAATCATTGAAAAAGTCAAAAGTGCCGCGCTGTCAAGGGATGCCGCAAGAAAAGCAAAAGACCTCACCAGAAGAAAA
TCGGCACTCGAAAGCTCAGGACTGCCCGGCAAACTCGCAGACTGCTCGATCAACGACCCCGAGCATTGTGAACTCTATAT
TGTTGAGGGTGACTCCGCCGGCGGGAGTGCAAAGCAGGGAAGAGACCGCAGCTTCCAGGCTATCCTGCCGCTCAAGGGCA
AAATTCTGAACGTCGAAAAAGCCCGTCTGCACAAAATGCTTGAGAATGAAGAGATAAAAACAATCATACTCGCGCTCGGC
ACAAGCTTCGGGGAAGAAGAATTTTCAGTGGAAAAACTACGGTACGGCAAAATAATCATCATGACTGATGCTGACGTGGA
CGGAGCCCACATCAGAACACTGCTCCTGACCTTCTTCTTCCGATACATGCGGTCCCTGATAGAGGCAGGCAAGGTCTTCA
TCGCCCAGCCTCCACTCTACCTCATCAAGTCAGGTAAGGAGCAAAAATACGCATGGGATGACGATGAACGCAACGGCATT
GTCGAGTCCATCAGGAAAAAGCAGAAAGGGAGAGGGAATATTAACATTCAGCGCTACAAAGGTCTGGGTGAAATGAACCC
TGAACAACTCTGGAGCACAACCATGGATCCGGAGCACCGTTCGCTGTTACAGGTGTCCGTTGAAAATGCCATGGAAGCCG
ATCAGGTCTTTTCGACACTTATGGGGGACAAGGTCGAACCACGCCGAAACTTTATTGAAAAAAACGCCCGCTATGTCAGA
CGTTTAGATGTCTGA

Upstream 100 bases:

>100_bases
CCGGACTCCCTGTCTCCGTGGGATATGAGGCTATTTTTTGCTATCTTGGAAAACAAAATATTTACACGATTTGAACCTGA
TTTTACCGCTATACAACACT

Downstream 100 bases:

>100_bases
GATCCTGACATAGACCTCTTTCTGCAGCTCATTGATCCATTCCTGGAACAATCTGTTCTGCTTCTCGTCAATGGCAAGTT
TTTCGAGCCTGGCATAATCC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MSEIQKNTSPQAYEATNIQILDGIEHVRKRPAMYIGDLHSRGLHHLIYEIVDNSIDETLAGYNESIELSLNPDGSVTVTD
NGRGIPVDIHPQKKKSALELVMTLIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYREGNIWFQRYHKGIPQRD
VEVIGESTKTGTKTTFMPDPDIFKITEFRKDIVVDRMRELAFLNNILKIVVKDIDGSEETFHYEGGIKEFVTFTDHNRIS
LLKEPVYISGERDSTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDLLKNLKLSLTGDDF
KEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNEQLSDFAESNPGVLKTIIEKVKSAALSRDAARKAKDLTRRK
SALESSGLPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
TSFGEEEFSVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLYLIKSGKEQKYAWDDDERNGI
VESIRKKQKGRGNINIQRYKGLGEMNPEQLWSTTMDPEHRSLLQVSVENAMEADQVFSTLMGDKVEPRRNFIEKNARYVR
RLDV

Sequences:

>Translated_644_residues
MSEIQKNTSPQAYEATNIQILDGIEHVRKRPAMYIGDLHSRGLHHLIYEIVDNSIDETLAGYNESIELSLNPDGSVTVTD
NGRGIPVDIHPQKKKSALELVMTLIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYREGNIWFQRYHKGIPQRD
VEVIGESTKTGTKTTFMPDPDIFKITEFRKDIVVDRMRELAFLNNILKIVVKDIDGSEETFHYEGGIKEFVTFTDHNRIS
LLKEPVYISGERDSTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDLLKNLKLSLTGDDF
KEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNEQLSDFAESNPGVLKTIIEKVKSAALSRDAARKAKDLTRRK
SALESSGLPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
TSFGEEEFSVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLYLIKSGKEQKYAWDDDERNGI
VESIRKKQKGRGNINIQRYKGLGEMNPEQLWSTTMDPEHRSLLQVSVENAMEADQVFSTLMGDKVEPRRNFIEKNARYVR
RLDV
>Mature_643_residues
SEIQKNTSPQAYEATNIQILDGIEHVRKRPAMYIGDLHSRGLHHLIYEIVDNSIDETLAGYNESIELSLNPDGSVTVTDN
GRGIPVDIHPQKKKSALELVMTLIGAGGKFDKGAYKVSGGLHGVGASVVNALSEWCEVEVYREGNIWFQRYHKGIPQRDV
EVIGESTKTGTKTTFMPDPDIFKITEFRKDIVVDRMRELAFLNNILKIVVKDIDGSEETFHYEGGIKEFVTFTDHNRISL
LKEPVYISGERDSTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNSYAQKNDLLKNLKLSLTGDDFK
EGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNEQLSDFAESNPGVLKTIIEKVKSAALSRDAARKAKDLTRRKS
ALESSGLPGKLADCSINDPEHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALGT
SFGEEEFSVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLYLIKSGKEQKYAWDDDERNGIV
ESIRKKQKGRGNINIQRYKGLGEMNPEQLWSTTMDPEHRSLLQVSVENAMEADQVFSTLMGDKVEPRRNFIEKNARYVRR
LDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=669, Percent_Identity=26.3079222720478, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI19913406, Length=613, Percent_Identity=25.7748776508972, Blast_Score=138, Evalue=2e-32,
Organism=Escherichia coli, GI48994957, Length=547, Percent_Identity=54.2961608775137, Blast_Score=589, Evalue=1e-169,
Organism=Escherichia coli, GI1789408, Length=632, Percent_Identity=39.2405063291139, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=26, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17535065, Length=658, Percent_Identity=24.9240121580547, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI212645657, Length=172, Percent_Identity=30.8139534883721, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=570, Percent_Identity=25.4385964912281, Blast_Score=123, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17136538, Length=680, Percent_Identity=24.1176470588235, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72288; Mature: 72157

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIQKNTSPQAYEATNIQILDGIEHVRKRPAMYIGDLHSRGLHHLIYEIVDNSIDETLA
CCCCCCCCCCCCEECCCEEEHHHHHHHHHCCCEEECCHHHCHHHHHHHHHHHCHHHHHHH
GYNESIELSLNPDGSVTVTDNGRGIPVDIHPQKKKSALELVMTLIGAGGKFDKGAYKVSG
CCCCEEEEEECCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECC
GLHGVGASVVNALSEWCEVEVYREGNIWFQRYHKGIPQRDVEVIGESTKTGTKTTFMPDP
CCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCC
DIFKITEFRKDIVVDRMRELAFLNNILKIVVKDIDGSEETFHYEGGIKEFVTFTDHNRIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHEECCCCCHH
LLKEPVYISGERDSTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNS
HCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
YAQKNDLLKNLKLSLTGDDFKEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNE
HHHHHHHHHHCEEEECCCHHHHHHEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHH
QLSDFAESNPGVLKTIIEKVKSAALSRDAARKAKDLTRRKSALESSGLPGKLADCSINDP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
CCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCHHHEEEEECC
TSFGEEEFSVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLY
CCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
LIKSGKEQKYAWDDDERNGIVESIRKKQKGRGNINIQRYKGLGEMNPEQLWSTTMDPEHR
EEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHH
SLLQVSVENAMEADQVFSTLMGDKVEPRRNFIEKNARYVRRLDV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIQKNTSPQAYEATNIQILDGIEHVRKRPAMYIGDLHSRGLHHLIYEIVDNSIDETLA
CCCCCCCCCCCEECCCEEEHHHHHHHHHCCCEEECCHHHCHHHHHHHHHHHCHHHHHHH
GYNESIELSLNPDGSVTVTDNGRGIPVDIHPQKKKSALELVMTLIGAGGKFDKGAYKVSG
CCCCEEEEEECCCCCEEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECC
GLHGVGASVVNALSEWCEVEVYREGNIWFQRYHKGIPQRDVEVIGESTKTGTKTTFMPDP
CCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCC
DIFKITEFRKDIVVDRMRELAFLNNILKIVVKDIDGSEETFHYEGGIKEFVTFTDHNRIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHEECCCCCHH
LLKEPVYISGERDSTVVEIALQYNDSYQENVFSYVNNINTHEGGTHVTGFRKALTRTLNS
HCCCCEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
YAQKNDLLKNLKLSLTGDDFKEGLTAIISVKVAEPQFEGQTKTKLGNSETQSIVESIVNE
HHHHHHHHHHCEEEECCCHHHHHHEEEEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHH
QLSDFAESNPGVLKTIIEKVKSAALSRDAARKAKDLTRRKSALESSGLPGKLADCSINDP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EHCELYIVEGDSAGGSAKQGRDRSFQAILPLKGKILNVEKARLHKMLENEEIKTIILALG
CCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHCCCHHHEEEEECC
TSFGEEEFSVEKLRYGKIIIMTDADVDGAHIRTLLLTFFFRYMRSLIEAGKVFIAQPPLY
CCCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
LIKSGKEQKYAWDDDERNGIVESIRKKQKGRGNINIQRYKGLGEMNPEQLWSTTMDPEHR
EEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHCCCCHHHH
SLLQVSVENAMEADQVFSTLMGDKVEPRRNFIEKNARYVRRLDV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]