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The map label for this gene is outF [H]

Identifier: 189424344

GI number: 189424344

Start: 1326703

End: 1327914

Strand: Reverse

Name: outF [H]

Synonym: Glov_1279

Alternate gene names: 189424344

Gene position: 1327914-1326703 (Counterclockwise)

Preceding gene: 189424345

Following gene: 189424343

Centisome position: 33.89

GC content: 61.96

Gene sequence:

>1212_bases
ATGTCGACCTTCCAGTATAACGGATTTTCTTCAGACGGTCGCGAGACCAAAGGGGTACTGGAGGCTGACAGCCTGCGGGC
TGCCCGCGAGGAACTGCGTCACCGCGGGGTCTTACCGACAACCCTCACCGAACAGGACACCAAAGCCCCCGCAGGTATCC
TGGGCTTAGGCCGCAAGGCGGTTTCTGTTGCCTCACTTGCCCTGTTTACCCGGCGTCTCTCCACCCTTACAGCAGCGGCG
GTACCGATCCATGAAGCGCTGGAAGCATTGCACCGCCAGGAAAAGACCGGGGAATTGCGTTCCATCCTGTCGCGTCTGCG
CAGCCGGCTGGCAGAAGGATCACCACTTGCCCGGGCCATGTCCTCAGAACCGAAGGTCTTTGGTGAAAGCTATATCGCCA
TGGTGGCAGCCGGTGAGGCGGGCGGAGCGCTTGACAAGGTACTACAGCGTCTGGCCGACTTTCTGGAACGCCAGGAGGAG
ATGCGCCGTTCAGTCAGCACCGCCCTGGCCTATCCGGGCCTGATGGCGGTGGTGGGCAGCGGCGTCATGCTCTTTCTGCT
GACCTTTGTCGTTCCCAAGATCACCGGCATCTTCGCCGACACCAAAGCCACGCTGCCGTTTTTGACGGTGGCGCTGCTGA
CCGTCAGCAACCTGGTTCGCAGCGGCTGGTGGCTGCTGTTGCTGCTGACCGTTGCCGCCCTGCTGACCTACCGCCGGCTG
TCTCTCAAGGAAAGTTTCCTGCAACGGCGCGACCGGTTTCTGGTGTCTCTGCCGCTGGTGGGGGGGCTGCTGCAGACGCT
GGCCCTGGCCCGTTTCTCACGGGTGCTGGGACTTTTGCTGGGGAGCGGCGTGCCGCTTCTGCATTCCCTTGAAATTACTT
CGGAGGCGGTGGTCAACCGGGCCTACCGCGCTGTCCTGCACGAGGCGGGACGTTCACTGGCAGAAGGAGGCAGTCTCTCC
GCCACCCTTGAGCGTTCCGAGCTGTTCCCGCCGGTCCTGACCCACCTGATTGCAGTGGGGGAACGCAGCGGTGCGCTGGT
TGACAGCCTGGAAACGGCAGGAAGCAGTTTTGAACGGGAGTTTGAGGCGGCAACCGGCCGTTTTATGGCCCTGCTGGAAC
CGCTGCTGGTGCTGGCGATGGGCCTGATGGTGGGACTGGTGGTGATTGCCGTCCTGTTACCGATTTTCCAGCTGAACGAA
CTGGTTAAATAG

Upstream 100 bases:

>100_bases
ATTTCATGCGGTATGCGAACGCTGCGGGAAGATGGTTTTGCCAAGGTAGCCGCAGGGATCACCACCCTGGAAGAGGTCCT
GAGGGTAACCCAGGAAGAGT

Downstream 100 bases:

>100_bases
ACGTTTGAAGGGAGAATAGACCGATGCAGAGAACAGTCAACAACCGGCGGGGTTTTACCCTGATAGAAATCATGGTGGTC
ATCGTCATACTGGCGCTTTT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Pectic enzymes secretion protein outF [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSTFQYNGFSSDGRETKGVLEADSLRAAREELRHRGVLPTTLTEQDTKAPAGILGLGRKAVSVASLALFTRRLSTLTAAA
VPIHEALEALHRQEKTGELRSILSRLRSRLAEGSPLARAMSSEPKVFGESYIAMVAAGEAGGALDKVLQRLADFLERQEE
MRRSVSTALAYPGLMAVVGSGVMLFLLTFVVPKITGIFADTKATLPFLTVALLTVSNLVRSGWWLLLLLTVAALLTYRRL
SLKESFLQRRDRFLVSLPLVGGLLQTLALARFSRVLGLLLGSGVPLLHSLEITSEAVVNRAYRAVLHEAGRSLAEGGSLS
ATLERSELFPPVLTHLIAVGERSGALVDSLETAGSSFEREFEAATGRFMALLEPLLVLAMGLMVGLVVIAVLLPIFQLNE
LVK

Sequences:

>Translated_403_residues
MSTFQYNGFSSDGRETKGVLEADSLRAAREELRHRGVLPTTLTEQDTKAPAGILGLGRKAVSVASLALFTRRLSTLTAAA
VPIHEALEALHRQEKTGELRSILSRLRSRLAEGSPLARAMSSEPKVFGESYIAMVAAGEAGGALDKVLQRLADFLERQEE
MRRSVSTALAYPGLMAVVGSGVMLFLLTFVVPKITGIFADTKATLPFLTVALLTVSNLVRSGWWLLLLLTVAALLTYRRL
SLKESFLQRRDRFLVSLPLVGGLLQTLALARFSRVLGLLLGSGVPLLHSLEITSEAVVNRAYRAVLHEAGRSLAEGGSLS
ATLERSELFPPVLTHLIAVGERSGALVDSLETAGSSFEREFEAATGRFMALLEPLLVLAMGLMVGLVVIAVLLPIFQLNE
LVK
>Mature_402_residues
STFQYNGFSSDGRETKGVLEADSLRAAREELRHRGVLPTTLTEQDTKAPAGILGLGRKAVSVASLALFTRRLSTLTAAAV
PIHEALEALHRQEKTGELRSILSRLRSRLAEGSPLARAMSSEPKVFGESYIAMVAAGEAGGALDKVLQRLADFLERQEEM
RRSVSTALAYPGLMAVVGSGVMLFLLTFVVPKITGIFADTKATLPFLTVALLTVSNLVRSGWWLLLLLTVAALLTYRRLS
LKESFLQRRDRFLVSLPLVGGLLQTLALARFSRVLGLLLGSGVPLLHSLEITSEAVVNRAYRAVLHEAGRSLAEGGSLSA
TLERSELFPPVLTHLIAVGERSGALVDSLETAGSSFEREFEAATGRFMALLEPLLVLAMGLMVGLVVIAVLLPIFQLNEL
VK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=405, Percent_Identity=33.8271604938272, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786295, Length=402, Percent_Identity=25.8706467661692, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 43470; Mature: 43338

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFQYNGFSSDGRETKGVLEADSLRAAREELRHRGVLPTTLTEQDTKAPAGILGLGRKA
CCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHH
VSVASLALFTRRLSTLTAAAVPIHEALEALHRQEKTGELRSILSRLRSRLAEGSPLARAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHH
SSEPKVFGESYIAMVAAGEAGGALDKVLQRLADFLERQEEMRRSVSTALAYPGLMAVVGS
CCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVMLFLLTFVVPKITGIFADTKATLPFLTVALLTVSNLVRSGWWLLLLLTVAALLTYRRL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLKESFLQRRDRFLVSLPLVGGLLQTLALARFSRVLGLLLGSGVPLLHSLEITSEAVVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AYRAVLHEAGRSLAEGGSLSATLERSELFPPVLTHLIAVGERSGALVDSLETAGSSFERE
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHH
FEAATGRFMALLEPLLVLAMGLMVGLVVIAVLLPIFQLNELVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STFQYNGFSSDGRETKGVLEADSLRAAREELRHRGVLPTTLTEQDTKAPAGILGLGRKA
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHH
VSVASLALFTRRLSTLTAAAVPIHEALEALHRQEKTGELRSILSRLRSRLAEGSPLARAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHH
SSEPKVFGESYIAMVAAGEAGGALDKVLQRLADFLERQEEMRRSVSTALAYPGLMAVVGS
CCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVMLFLLTFVVPKITGIFADTKATLPFLTVALLTVSNLVRSGWWLLLLLTVAALLTYRRL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLKESFLQRRDRFLVSLPLVGGLLQTLALARFSRVLGLLLGSGVPLLHSLEITSEAVVNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AYRAVLHEAGRSLAEGGSLSATLERSELFPPVLTHLIAVGERSGALVDSLETAGSSFERE
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHH
FEAATGRFMALLEPLLVLAMGLMVGLVVIAVLLPIFQLNELVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]