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The map label for this gene is epsE [H]

Identifier: 189424345

GI number: 189424345

Start: 1327895

End: 1329460

Strand: Reverse

Name: epsE [H]

Synonym: Glov_1280

Alternate gene names: 189424345

Gene position: 1329460-1327895 (Counterclockwise)

Preceding gene: 189424346

Following gene: 189424344

Centisome position: 33.93

GC content: 60.54

Gene sequence:

>1566_bases
ATGAGCCCCGAGCACGCCCTGGACAACCTTAAGACTGCGGCTGAGCGCCTGAACCTGCCTTTTCTGGAGCGGATTGACGA
CCAGCAGGCCAACAGCTCGTTACTGGCCAGACTCCCGCTTGCCTTTGCCCGCAGCAAGCTGCTGTTGCCGCTGCAGGAAA
AAGAGGACCGCCTGCTGCTTGCCGTGGCCAACCCTACGGACCTGCTGGCCCAGGATGAGGTGGCCAAGCTGTATAACCTG
CCGCTCTCCGTGCTGGTGGTACCGCCGGATGAGCTGCTGGCAGCAATCAACCGGCTCTACAGCCGCACAGCCGGATCGGC
CCGTGAGGTGGTGGAGAATCTGGCCGATGAAGACCTCTCGGTCATCGCCACCGAGCTGGCCCATCCCAAGGATCTGCTTG
ATTTAAGCGATGAGGCACCGGTCATCCGTCTCCTGAATGCGATCCTCTTCCAGGCGGTCAAGGAACGAGCCAGCGACATC
CATATCGAACCGTTTGAACGGACCCTAGATGTCCGTTTTCGGGTTGACGGCATTCTGCATCAGGTCCTGGAACCGCCCAA
GGTGCTGCAGGAGGCGCTGGTGTCGCGGGTCAAGATCATGGCCGCGCTGAATATCGCGGAAAAGCGCCTGCCCCAGGATG
GCCGTTTCAAGGTGCTGGTGGCAGGCCATGAGGTGGATATCCGGGTCTCGATCATCCCGACCTTTTTCGGCGAGCGGACC
GTACTGCGTCTGCTTGACCGCCACAAGGGAATCCTGTCACTGACCGACATCGGTTTTTCCGAGCGGGATCGCGACACCAT
GCAGCGTCTGATCAGCCGCTCCAGCGGCATCATCCTGGTGACCGGCCCCACCGGCAGCGGCAAGACCACCACCCTCTATG
CCGCCGTCTCACGCCTGAACCGCCAGGAAAAGAACCTGATCACCATTGAAGACCCGATTGAATACCAGTTGTCCGGTGTC
GGCCAGATACAGGTCAACCCGAAGATCGACCTGACCTTTGCCAACGGACTGCGTTCAATCCTGCGCCAGGACCCGGACAT
CATCATGGTGGGCGAGATCCGTGATGCCGAAACCGCCGAGATTGCCATGCAGGCCTCGCTGACCGGCCACCTGGTACTCT
CGACCCTGCATACCAACGATGCTGCCACGGCCGTTACCAGATTGATGGACATGGGGATTGAGCCGTTTATGGTGGCTTCA
TCCCTGGCGGGCATCCTGGCACAACGCCTGGTACGGACCATCTGCCCCCACTGCCGCGAGTCCTACCGGCCGGAACAGCC
GGTTGAGGGACTGCCGGAGCTGCTCTACCGCGGCCGGGGCTGTGACCACTGCTTCGGGCAGGGGACTCTGGGCAGAACCG
GGATCTACGAACTGCTGACCATTGATTCCGAGCTGTGCAGCATGATCACCAGACAGGCCCCGGCCGGTGAAATCAAGGAA
TATGCCATTTCATGCGGTATGCGAACGCTGCGGGAAGATGGTTTTGCCAAGGTAGCCGCAGGGATCACCACCCTGGAAGA
GGTCCTGAGGGTAACCCAGGAAGAGTATGTCGACCTTCCAGTATAA

Upstream 100 bases:

>100_bases
TCACTGAAGCTGCCAAAGAGTTCGGCCCGCTGAATGTGCAGAATGAGATCTCAGGCAAAGCGACCAAGCCGGTAAGCCAA
GGGCAGGAGAGCCGGCCCTG

Downstream 100 bases:

>100_bases
CGGATTTTCTTCAGACGGTCGCGAGACCAAAGGGGTACTGGAGGCTGACAGCCTGCGGGCTGCCCGCGAGGAACTGCGTC
ACCGCGGGGTCTTACCGACA

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSPEHALDNLKTAAERLNLPFLERIDDQQANSSLLARLPLAFARSKLLLPLQEKEDRLLLAVANPTDLLAQDEVAKLYNL
PLSVLVVPPDELLAAINRLYSRTAGSAREVVENLADEDLSVIATELAHPKDLLDLSDEAPVIRLLNAILFQAVKERASDI
HIEPFERTLDVRFRVDGILHQVLEPPKVLQEALVSRVKIMAALNIAEKRLPQDGRFKVLVAGHEVDIRVSIIPTFFGERT
VLRLLDRHKGILSLTDIGFSERDRDTMQRLISRSSGIILVTGPTGSGKTTTLYAAVSRLNRQEKNLITIEDPIEYQLSGV
GQIQVNPKIDLTFANGLRSILRQDPDIIMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDAATAVTRLMDMGIEPFMVAS
SLAGILAQRLVRTICPHCRESYRPEQPVEGLPELLYRGRGCDHCFGQGTLGRTGIYELLTIDSELCSMITRQAPAGEIKE
YAISCGMRTLREDGFAKVAAGITTLEEVLRVTQEEYVDLPV

Sequences:

>Translated_521_residues
MSPEHALDNLKTAAERLNLPFLERIDDQQANSSLLARLPLAFARSKLLLPLQEKEDRLLLAVANPTDLLAQDEVAKLYNL
PLSVLVVPPDELLAAINRLYSRTAGSAREVVENLADEDLSVIATELAHPKDLLDLSDEAPVIRLLNAILFQAVKERASDI
HIEPFERTLDVRFRVDGILHQVLEPPKVLQEALVSRVKIMAALNIAEKRLPQDGRFKVLVAGHEVDIRVSIIPTFFGERT
VLRLLDRHKGILSLTDIGFSERDRDTMQRLISRSSGIILVTGPTGSGKTTTLYAAVSRLNRQEKNLITIEDPIEYQLSGV
GQIQVNPKIDLTFANGLRSILRQDPDIIMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDAATAVTRLMDMGIEPFMVAS
SLAGILAQRLVRTICPHCRESYRPEQPVEGLPELLYRGRGCDHCFGQGTLGRTGIYELLTIDSELCSMITRQAPAGEIKE
YAISCGMRTLREDGFAKVAAGITTLEEVLRVTQEEYVDLPV
>Mature_520_residues
SPEHALDNLKTAAERLNLPFLERIDDQQANSSLLARLPLAFARSKLLLPLQEKEDRLLLAVANPTDLLAQDEVAKLYNLP
LSVLVVPPDELLAAINRLYSRTAGSAREVVENLADEDLSVIATELAHPKDLLDLSDEAPVIRLLNAILFQAVKERASDIH
IEPFERTLDVRFRVDGILHQVLEPPKVLQEALVSRVKIMAALNIAEKRLPQDGRFKVLVAGHEVDIRVSIIPTFFGERTV
LRLLDRHKGILSLTDIGFSERDRDTMQRLISRSSGIILVTGPTGSGKTTTLYAAVSRLNRQEKNLITIEDPIEYQLSGVG
QIQVNPKIDLTFANGLRSILRQDPDIIMVGEIRDAETAEIAMQASLTGHLVLSTLHTNDAATAVTRLMDMGIEPFMVASS
LAGILAQRLVRTICPHCRESYRPEQPVEGLPELLYRGRGCDHCFGQGTLGRTGIYELLTIDSELCSMITRQAPAGEIKEY
AISCGMRTLREDGFAKVAAGITTLEEVLRVTQEEYVDLPV

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=433, Percent_Identity=48.0369515011547, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1786296, Length=376, Percent_Identity=43.3510638297872, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI87082188, Length=151, Percent_Identity=47.0198675496689, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57645; Mature: 57514

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPEHALDNLKTAAERLNLPFLERIDDQQANSSLLARLPLAFARSKLLLPLQEKEDRLLL
CCCHHHHHHHHHHHHHCCCCHHHHCCCHHCCHHHHHHHHHHHHHCHHCCCCCCCCCCEEE
AVANPTDLLAQDEVAKLYNLPLSVLVVPPDELLAAINRLYSRTAGSAREVVENLADEDLS
EECCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHH
VIATELAHPKDLLDLSDEAPVIRLLNAILFQAVKERASDIHIEPFERTLDVRFRVDGILH
HHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHH
QVLEPPKVLQEALVSRVKIMAALNIAEKRLPQDGRFKVLVAGHEVDIRVSIIPTFFGERT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCHHH
VLRLLDRHKGILSLTDIGFSERDRDTMQRLISRSSGIILVTGPTGSGKTTTLYAAVSRLN
HHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHC
RQEKNLITIEDPIEYQLSGVGQIQVNPKIDLTFANGLRSILRQDPDIIMVGEIRDAETAE
HHHCCEEEEECCCHHHCCCCEEEEECCEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHH
IAMQASLTGHLVLSTLHTNDAATAVTRLMDMGIEPFMVASSLAGILAQRLVRTICPHCRE
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SYRPEQPVEGLPELLYRGRGCDHCFGQGTLGRTGIYELLTIDSELCSMITRQAPAGEIKE
HCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCHHHHH
YAISCGMRTLREDGFAKVAAGITTLEEVLRVTQEEYVDLPV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SPEHALDNLKTAAERLNLPFLERIDDQQANSSLLARLPLAFARSKLLLPLQEKEDRLLL
CCHHHHHHHHHHHHHCCCCHHHHCCCHHCCHHHHHHHHHHHHHCHHCCCCCCCCCCEEE
AVANPTDLLAQDEVAKLYNLPLSVLVVPPDELLAAINRLYSRTAGSAREVVENLADEDLS
EECCCHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHH
VIATELAHPKDLLDLSDEAPVIRLLNAILFQAVKERASDIHIEPFERTLDVRFRVDGILH
HHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHH
QVLEPPKVLQEALVSRVKIMAALNIAEKRLPQDGRFKVLVAGHEVDIRVSIIPTFFGERT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCHHH
VLRLLDRHKGILSLTDIGFSERDRDTMQRLISRSSGIILVTGPTGSGKTTTLYAAVSRLN
HHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHC
RQEKNLITIEDPIEYQLSGVGQIQVNPKIDLTFANGLRSILRQDPDIIMVGEIRDAETAE
HHHCCEEEEECCCHHHCCCCEEEEECCEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHH
IAMQASLTGHLVLSTLHTNDAATAVTRLMDMGIEPFMVASSLAGILAQRLVRTICPHCRE
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SYRPEQPVEGLPELLYRGRGCDHCFGQGTLGRTGIYELLTIDSELCSMITRQAPAGEIKE
HCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCHHHHH
YAISCGMRTLREDGFAKVAAGITTLEEVLRVTQEEYVDLPV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]