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The map label for this gene is vnfH [H]

Identifier: 189424283

GI number: 189424283

Start: 1263384

End: 1264250

Strand: Reverse

Name: vnfH [H]

Synonym: Glov_1218

Alternate gene names: 189424283

Gene position: 1264250-1263384 (Counterclockwise)

Preceding gene: 189424284

Following gene: 189424282

Centisome position: 32.27

GC content: 56.4

Gene sequence:

>867_bases
ATGAGACAGGTAGCAATCTACGGCAAAGGCGGCATCGGCAAATCCACCACCACCCAGAACACCGTGGCAGGGCTGGCATC
AATGGGCAAAAAGGTCCTGATCGTCGGCTGCGACCCAAAGGCGGATTCAACCCGTCTGATCCTGCATGCCAAGGCCCAGA
ATACCGTAATGGATCTGGTTCGCGAGCTGGGTACGGTTGAAGATCTTGAACTTGATGCCGTATTGAAAGAGGGATACGCC
GGCATCAAGTGCGTCGAGTCAGGCGGCCCTGAGCCGGGTGTTGGCTGTGCCGGGCGCGGCGTCATCACCGCCATCAACTT
TCTGGAAGAAAATGGCGCCTATACCGATGACCTTGATTTTGTTTTCTACGATGTTCTGGGCGACGTTGTCTGCGGCGGGT
TTGCCATGCCGATCCGTGAAGGCAAGGCCGAAGAGATCTATATCGTTACCTCCGGTGAAATGATGGCCATGTACGCAGCC
AACAATATTTCCAAGGGTATCCTCAAATACGCCTCTTCAGGCAAGGTTCGCCTGGCCGGACTGATCTGCAACGCCCGCAA
GACCGACAAGGAGTTCGAACTGATCTCGGCCCTGGCGCGCAAGCTGAGCACCCAGATGATCCACTTTGTTCCCCGTGACA
ACCAGGTCCAGCGGGCTGAACTGCGCAGAATGACGGTCATCGAATATTCTCCTGAACATCCCCAGGCACAGGAATACCTG
ACCCTGGCCCAGAAGATTGCCGACAACAAGATGCTGGTCATCCCGACCCCGCTGGAGATGGAGGAGCTGGAAGAGCTGTT
GATGGAGTTCGGGATCATGGAGGCCGAAGACGAAAGCGTGGTTGGCGTGGCAGAAGCAGCAGCCTGA

Upstream 100 bases:

>100_bases
AAAGCCAGGGGATCATGGGGCTGCTTCAGCACCGTGCGGCGGCTTCTTCTGCTACCTCAAACAGCTCTGGTTCACTGCGG
TGAACCGAAGGGAGACGAGT

Downstream 100 bases:

>100_bases
CAATCCTGCACTCAGCCAGGTAATAACGGCACAGGGGCCAGCGATCTATCGTTGGCCCCTGTGCCGTTTCTGTGCTACAG
AAGTGGCCAGAAACCAGCCG

Product: nitrogenase iron protein

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRQVAIYGKGGIGKSTTTQNTVAGLASMGKKVLIVGCDPKADSTRLILHAKAQNTVMDLVRELGTVEDLELDAVLKEGYA
GIKCVESGGPEPGVGCAGRGVITAINFLEENGAYTDDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVTSGEMMAMYAA
NNISKGILKYASSGKVRLAGLICNARKTDKEFELISALARKLSTQMIHFVPRDNQVQRAELRRMTVIEYSPEHPQAQEYL
TLAQKIADNKMLVIPTPLEMEELEELLMEFGIMEAEDESVVGVAEAAA

Sequences:

>Translated_288_residues
MRQVAIYGKGGIGKSTTTQNTVAGLASMGKKVLIVGCDPKADSTRLILHAKAQNTVMDLVRELGTVEDLELDAVLKEGYA
GIKCVESGGPEPGVGCAGRGVITAINFLEENGAYTDDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVTSGEMMAMYAA
NNISKGILKYASSGKVRLAGLICNARKTDKEFELISALARKLSTQMIHFVPRDNQVQRAELRRMTVIEYSPEHPQAQEYL
TLAQKIADNKMLVIPTPLEMEELEELLMEFGIMEAEDESVVGVAEAAA
>Mature_288_residues
MRQVAIYGKGGIGKSTTTQNTVAGLASMGKKVLIVGCDPKADSTRLILHAKAQNTVMDLVRELGTVEDLELDAVLKEGYA
GIKCVESGGPEPGVGCAGRGVITAINFLEENGAYTDDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVTSGEMMAMYAA
NNISKGILKYASSGKVRLAGLICNARKTDKEFELISALARKLSTQMIHFVPRDNQVQRAELRRMTVIEYSPEHPQAQEYL
TLAQKIADNKMLVIPTPLEMEELEELLMEFGIMEAEDESVVGVAEAAA

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31189; Mature: 31189

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQVAIYGKGGIGKSTTTQNTVAGLASMGKKVLIVGCDPKADSTRLILHAKAQNTVMDLV
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHH
RELGTVEDLELDAVLKEGYAGIKCVESGGPEPGVGCAGRGVITAINFLEENGAYTDDLDF
HHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
VFYDVLGDVVCGGFAMPIREGKAEEIYIVTSGEMMAMYAANNISKGILKYASSGKVRLAG
HHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEEEE
LICNARKTDKEFELISALARKLSTQMIHFVPRDNQVQRAELRRMTVIEYSPEHPQAQEYL
EEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHEEEEEEECCCCCCHHHHH
TLAQKIADNKMLVIPTPLEMEELEELLMEFGIMEAEDESVVGVAEAAA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHCC
>Mature Secondary Structure
MRQVAIYGKGGIGKSTTTQNTVAGLASMGKKVLIVGCDPKADSTRLILHAKAQNTVMDLV
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCHHHHHHHH
RELGTVEDLELDAVLKEGYAGIKCVESGGPEPGVGCAGRGVITAINFLEENGAYTDDLDF
HHHCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHH
VFYDVLGDVVCGGFAMPIREGKAEEIYIVTSGEMMAMYAANNISKGILKYASSGKVRLAG
HHHHHHHHHHHCCEEECCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCEEEEE
LICNARKTDKEFELISALARKLSTQMIHFVPRDNQVQRAELRRMTVIEYSPEHPQAQEYL
EEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHEEEEEEECCCCCCHHHHH
TLAQKIADNKMLVIPTPLEMEELEELLMEFGIMEAEDESVVGVAEAAA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2388847 [H]