Click here to switch to the map view.

The map label for this gene is ynjE [C]

Identifier: 189424270

GI number: 189424270

Start: 1249927

End: 1251279

Strand: Reverse

Name: ynjE [C]

Synonym: Glov_1205

Alternate gene names: 189424270

Gene position: 1251279-1249927 (Counterclockwise)

Preceding gene: 189424275

Following gene: 189424269

Centisome position: 31.94

GC content: 56.25

Gene sequence:

>1353_bases
ATGATGCAACGTTTTGTGATGACAAGAAGGAGCATGCTGTTCAGTCTGGCCGGCTTACTGCTGACCACCGCACTGACACT
GTGGGGTTGCGGAGGTGGAGGCGGAACCAGCAGCTACACTGACCCCAATGCCTCAATCACAACGACAAAAACAGCAACGG
CACTGATTGAACCGGCAACCCTTAAACAATGGATGGATGAAGGCAAGGTCAACAACCCCGACCCTGCAACCCGTGATCGG
GTAGTGATTGTATCAGTAGGAACCCCGGCCAACTATGCCACAGCCCACATCCCCGGTTCGCAGTTACTGGACTCCGGGGC
ACTGAACCTGACCCGCCTGGAAGGTGTCACTGCGATCGGCACCATGGTTATGGACGGCCCGACCATTGACGGACATATCC
AAAGACTCGGTATCAACCAGTATACGACGATTGTCATTGCCCCGACAGGGACCGACACAGCCATGAACGCATCCAGGGTC
TATTTCACACTTCGCTACTGGGGCTTCCCCAAAGAACGCGTAAAGATCCTCAACGGTGGCCAGACCGCCTGGACAACTGC
GGCGGCTACATGGCCGGCAGCCTACGCGGTGACAGCAACCGTACCGACCATCCCGGCCTCCACTTTCAGCGTCAAGAACC
TGTATAACGGCACAACCACCACCAACTTTAACCTGCGTTACTCAATCGGCGAAATGATCAACCTTGTGGACAAGATCAAT
GCCGGAACCATCAGCACCGGCGCAACCGGTGTCGCCATCATGGATGAGCGTGGCGGTGTCAACGTGACCACTCACCCGTA
CATTGCCAATGCAGCCACCTATAACAACGGAACCACCACTGTCCTTGACAGCCATATGAACTATTACGGCACCGTGGCAG
GAACATTCAAGGATACCGCTGCATTAACCGCCAATCTTGCCAATTTTGGCATAACCGCCAACAAAACCATGACCTACCTC
TACTGCGCCAGCGGCATGCGTGCCTCAACGGCATTCTTTGTCCTGGACGGCATCCTCGGCTGGCCGGTCACCCTGTATGA
CGGTTCATGGGGCCAATGGAGCGGTTACACATCCCTCTCAACAAGCCCGGTGGCTACGGCATGGCGCACCAATGTCAACA
CCTCTGCAACAGTGGCAATAAACAGAACCGCAAATGCTTCTCTGTATGATCCTGCCGGCACCGGCAACGTCAAGCCGGCA
ACACTAACCATAGACCCAATCGCTAATGCCATCTATTCAACCATCACCGATCCCCGCGCCAACCAGATGCTGAACGAAGA
CAAGGCCTACTTCACCAGCGGCGGCACAACAACCGGCGGTGGCGGCGGCGGTGGCACCGGCAGCGGCTGCTGA

Upstream 100 bases:

>100_bases
TGAATACCTTACATGTAAGATCAGTCCGCGTACGCATGTCACAGGAAACCTGCGTATGCCCCAACCAACGGCCGACAGGC
CAAAACCTCAGGAGGTGTGT

Downstream 100 bases:

>100_bases
TCCAGCCGTAGAGTCCATCGGATAACCAGTCTATGACTCAGCACATATCAAAGGAGAAATCTTCTATGACTAAAATCGGT
AAAGTAAGCGCTGCAGCCGC

Product: rhodanese-like protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: Rhodanese Domain-Containing Protein; Rhodanese Domain Protein; Rhodanese Homology Domain Pair Protein; Lipoprotein

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MMQRFVMTRRSMLFSLAGLLLTTALTLWGCGGGGGTSSYTDPNASITTTKTATALIEPATLKQWMDEGKVNNPDPATRDR
VVIVSVGTPANYATAHIPGSQLLDSGALNLTRLEGVTAIGTMVMDGPTIDGHIQRLGINQYTTIVIAPTGTDTAMNASRV
YFTLRYWGFPKERVKILNGGQTAWTTAAATWPAAYAVTATVPTIPASTFSVKNLYNGTTTTNFNLRYSIGEMINLVDKIN
AGTISTGATGVAIMDERGGVNVTTHPYIANAATYNNGTTTVLDSHMNYYGTVAGTFKDTAALTANLANFGITANKTMTYL
YCASGMRASTAFFVLDGILGWPVTLYDGSWGQWSGYTSLSTSPVATAWRTNVNTSATVAINRTANASLYDPAGTGNVKPA
TLTIDPIANAIYSTITDPRANQMLNEDKAYFTSGGTTTGGGGGGGTGSGC

Sequences:

>Translated_450_residues
MMQRFVMTRRSMLFSLAGLLLTTALTLWGCGGGGGTSSYTDPNASITTTKTATALIEPATLKQWMDEGKVNNPDPATRDR
VVIVSVGTPANYATAHIPGSQLLDSGALNLTRLEGVTAIGTMVMDGPTIDGHIQRLGINQYTTIVIAPTGTDTAMNASRV
YFTLRYWGFPKERVKILNGGQTAWTTAAATWPAAYAVTATVPTIPASTFSVKNLYNGTTTTNFNLRYSIGEMINLVDKIN
AGTISTGATGVAIMDERGGVNVTTHPYIANAATYNNGTTTVLDSHMNYYGTVAGTFKDTAALTANLANFGITANKTMTYL
YCASGMRASTAFFVLDGILGWPVTLYDGSWGQWSGYTSLSTSPVATAWRTNVNTSATVAINRTANASLYDPAGTGNVKPA
TLTIDPIANAIYSTITDPRANQMLNEDKAYFTSGGTTTGGGGGGGTGSGC
>Mature_450_residues
MMQRFVMTRRSMLFSLAGLLLTTALTLWGCGGGGGTSSYTDPNASITTTKTATALIEPATLKQWMDEGKVNNPDPATRDR
VVIVSVGTPANYATAHIPGSQLLDSGALNLTRLEGVTAIGTMVMDGPTIDGHIQRLGINQYTTIVIAPTGTDTAMNASRV
YFTLRYWGFPKERVKILNGGQTAWTTAAATWPAAYAVTATVPTIPASTFSVKNLYNGTTTTNFNLRYSIGEMINLVDKIN
AGTISTGATGVAIMDERGGVNVTTHPYIANAATYNNGTTTVLDSHMNYYGTVAGTFKDTAALTANLANFGITANKTMTYL
YCASGMRASTAFFVLDGILGWPVTLYDGSWGQWSGYTSLSTSPVATAWRTNVNTSATVAINRTANASLYDPAGTGNVKPA
TLTIDPIANAIYSTITDPRANQMLNEDKAYFTSGGTTTGGGGGGGTGSGC

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 47222; Mature: 47222

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50206 RHODANESE_3 ; PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQRFVMTRRSMLFSLAGLLLTTALTLWGCGGGGGTSSYTDPNASITTTKTATALIEPAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHEEECHHH
LKQWMDEGKVNNPDPATRDRVVIVSVGTPANYATAHIPGSQLLDSGALNLTRLEGVTAIG
HHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCHHHHHCCCCCEEEEECCCEEEE
TMVMDGPTIDGHIQRLGINQYTTIVIAPTGTDTAMNASRVYFTLRYWGFPKERVKILNGG
EEEECCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHEEEEECC
QTAWTTAAATWPAAYAVTATVPTIPASTFSVKNLYNGTTTTNFNLRYSIGEMINLVDKIN
CCEEEEECCCCCCEEEEEEECCCCCCCCEEHHHCCCCCEEEEEEEEECHHHHHHHHHHCC
AGTISTGATGVAIMDERGGVNVTTHPYIANAATYNNGTTTVLDSHMNYYGTVAGTFKDTA
CCEECCCCCEEEEEECCCCEEEEECCEEEEEEEECCCCEEEEECCCCEEEEEEECCCCHH
ALTANLANFGITANKTMTYLYCASGMRASTAFFVLDGILGWPVTLYDGSWGQWSGYTSLS
HHEEHHHHCCEECCCEEEEEEECCCCCCCCCEEEEHHHCCCEEEEEECCCCCCCCCCCCC
TSPVATAWRTNVNTSATVAINRTANASLYDPAGTGNVKPATLTIDPIANAIYSTITDPRA
CCCCCEEEECCCCCEEEEEEECCCCCEEECCCCCCCCCEEEEEECHHHHHHHHHCCCCHH
NQMLNEDKAYFTSGGTTTGGGGGGGTGSGC
HHHHCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMQRFVMTRRSMLFSLAGLLLTTALTLWGCGGGGGTSSYTDPNASITTTKTATALIEPAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCHHEEECHHH
LKQWMDEGKVNNPDPATRDRVVIVSVGTPANYATAHIPGSQLLDSGALNLTRLEGVTAIG
HHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCHHHHHCCCCCEEEEECCCEEEE
TMVMDGPTIDGHIQRLGINQYTTIVIAPTGTDTAMNASRVYFTLRYWGFPKERVKILNGG
EEEECCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCEEEEEEEEECCCCHHHEEEEECC
QTAWTTAAATWPAAYAVTATVPTIPASTFSVKNLYNGTTTTNFNLRYSIGEMINLVDKIN
CCEEEEECCCCCCEEEEEEECCCCCCCCEEHHHCCCCCEEEEEEEEECHHHHHHHHHHCC
AGTISTGATGVAIMDERGGVNVTTHPYIANAATYNNGTTTVLDSHMNYYGTVAGTFKDTA
CCEECCCCCEEEEEECCCCEEEEECCEEEEEEEECCCCEEEEECCCCEEEEEEECCCCHH
ALTANLANFGITANKTMTYLYCASGMRASTAFFVLDGILGWPVTLYDGSWGQWSGYTSLS
HHEEHHHHCCEECCCEEEEEEECCCCCCCCCEEEEHHHCCCEEEEEECCCCCCCCCCCCC
TSPVATAWRTNVNTSATVAINRTANASLYDPAGTGNVKPATLTIDPIANAIYSTITDPRA
CCCCCEEEECCCCCEEEEEEECCCCCEEECCCCCCCCCEEEEEECHHHHHHHHHCCCCHH
NQMLNEDKAYFTSGGTTTGGGGGGGTGSGC
HHHHCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4 {thiosulfate}} [C]

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: Sulfur atom transfer [C]

Inhibitor: 2-Naphthalene sulfonate; 2-Oxoglutarate; Anions; CaCl2; CN-; Dinitrobenzene; Dithiothreitol; DL-Dihydrolipoate; Phenyl glyoxal; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA