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The map label for this gene is cysA [H]

Identifier: 189424226

GI number: 189424226

Start: 1214643

End: 1215587

Strand: Reverse

Name: cysA [H]

Synonym: Glov_1161

Alternate gene names: 189424226

Gene position: 1215587-1214643 (Counterclockwise)

Preceding gene: 189424227

Following gene: 189424218

Centisome position: 31.03

GC content: 48.36

Gene sequence:

>945_bases
ATGAAATTGAAAATCTTTGCACTGGCCCTGATCACTCTGTTCAGCATGAACAGCGCAGCACTGGCAATCAGCCTGGTTGA
TGCCGACTGGCTGAAGAAGAACATGAACAACAAGAACGTCAAGATCATGGATGTGGCTGACAAGCCGAATGCCTTTGACA
AGGAGCATATCCCCGGAGCTATCCAGGTCAAACGCTATCTTGATCTCTCCAACACTGATCGTTATCCAGCCACCGCCTAC
CCAACCAAGTTGCAGTTTGAGAAATTTCTCTCTGAGTCGGGAATCGAAAACGGTTCCGTGATTGTTGCCTACGACGACAA
GTCAAGTCTGTTTGCTACCCGCTTGCTGTTTCTGTTGGAGATGTTTGGCCACGACCCAGCCAAGCTGAAGCTGCTGAACG
GCGGAATTGTCAACTGGAAGAAACTCGGTAATAAGGTAACGGATGATGTAACTAAAATCAAAAAAAGCAAGTACAAGATT
GCTAAGACAAAAACTGACATGACTGTCACCTGGAGTGACATCTACCGTGATGTGGCCCAAGGGGCAAAGCCTGAGGTACT
GCTGCTGGATTCCCGGCCGGAAAACGAGTTCAAGGCCCAGAATATCCGTGCCATCCGTGGCGGTTATCTACCCAAAGCGG
TACTGGTGACCAGTTCCGATGCCAATGAAAAAGACAGCCAGCTGTTCAAGTCACTTGAAGACATCAAGAAGCTGTATGAG
GCAAAAGATGTCAACGGAGATCGTACGATCTATACCTACTGCCACAGTGGTGACCGCTCGGCACACACCTATGTTATCCT
CAAGTATATGCTCGGCTACAAAAACGTAAAAGTATATGAAGGTGGCTGGGTAGAGTGGGCAAATATGGTAGCCCTGCCAA
TGGCTGGTCAGATCTGGTTGTGGGATGCCCCTAAACCGGTCAAGAAGGAAGAACCAAAAAAGTAA

Upstream 100 bases:

>100_bases
TGATCCGTCTAACCTTTGTCTACGCCGGACAGATCAGCAAACTGGTCTGACAATCTACTTTTCACTGCCCATCGGGCACT
ATCCTGAAGGAGGTAACATA

Downstream 100 bases:

>100_bases
CTAGTCACTGGTTGGCACAGATCAGACAGTGACCTTTTAACGATTCTTCAGTTTAATGCGGGCTTGACGTAATTGTCAGG
CCCGCATCAGTTGCTCTATG

Product: rhodanese

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKLKIFALALITLFSMNSAALAISLVDADWLKKNMNNKNVKIMDVADKPNAFDKEHIPGAIQVKRYLDLSNTDRYPATAY
PTKLQFEKFLSESGIENGSVIVAYDDKSSLFATRLLFLLEMFGHDPAKLKLLNGGIVNWKKLGNKVTDDVTKIKKSKYKI
AKTKTDMTVTWSDIYRDVAQGAKPEVLLLDSRPENEFKAQNIRAIRGGYLPKAVLVTSSDANEKDSQLFKSLEDIKKLYE
AKDVNGDRTIYTYCHSGDRSAHTYVILKYMLGYKNVKVYEGGWVEWANMVALPMAGQIWLWDAPKPVKKEEPKK

Sequences:

>Translated_314_residues
MKLKIFALALITLFSMNSAALAISLVDADWLKKNMNNKNVKIMDVADKPNAFDKEHIPGAIQVKRYLDLSNTDRYPATAY
PTKLQFEKFLSESGIENGSVIVAYDDKSSLFATRLLFLLEMFGHDPAKLKLLNGGIVNWKKLGNKVTDDVTKIKKSKYKI
AKTKTDMTVTWSDIYRDVAQGAKPEVLLLDSRPENEFKAQNIRAIRGGYLPKAVLVTSSDANEKDSQLFKSLEDIKKLYE
AKDVNGDRTIYTYCHSGDRSAHTYVILKYMLGYKNVKVYEGGWVEWANMVALPMAGQIWLWDAPKPVKKEEPKK
>Mature_314_residues
MKLKIFALALITLFSMNSAALAISLVDADWLKKNMNNKNVKIMDVADKPNAFDKEHIPGAIQVKRYLDLSNTDRYPATAY
PTKLQFEKFLSESGIENGSVIVAYDDKSSLFATRLLFLLEMFGHDPAKLKLLNGGIVNWKKLGNKVTDDVTKIKKSKYKI
AKTKTDMTVTWSDIYRDVAQGAKPEVLLLDSRPENEFKAQNIRAIRGGYLPKAVLVTSSDANEKDSQLFKSLEDIKKLYE
AKDVNGDRTIYTYCHSGDRSAHTYVILKYMLGYKNVKVYEGGWVEWANMVALPMAGQIWLWDAPKPVKKEEPKK

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=296, Percent_Identity=23.9864864864865, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI61835204, Length=292, Percent_Identity=24.3150684931507, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI194473681, Length=292, Percent_Identity=24.3150684931507, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI17402865, Length=292, Percent_Identity=23.6301369863014, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87082121, Length=286, Percent_Identity=23.4265734265734, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=248, Percent_Identity=25, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=319, Percent_Identity=21.9435736677116, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 35557; Mature: 35557

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKIFALALITLFSMNSAALAISLVDADWLKKNMNNKNVKIMDVADKPNAFDKEHIPGA
CCEEHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCEEEEEECCCCCCCCHHCCCCC
IQVKRYLDLSNTDRYPATAYPTKLQFEKFLSESGIENGSVIVAYDDKSSLFATRLLFLLE
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
MFGHDPAKLKLLNGGIVNWKKLGNKVTDDVTKIKKSKYKIAKTKTDMTVTWSDIYRDVAQ
HHCCCCCEEEEECCCCEEHHHHCCHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHC
GAKPEVLLLDSRPENEFKAQNIRAIRGGYLPKAVLVTSSDANEKDSQLFKSLEDIKKLYE
CCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AKDVNGDRTIYTYCHSGDRSAHTYVILKYMLGYKNVKVYEGGWVEWANMVALPMAGQIWL
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCEEEHHEEEECCCCEEEE
WDAPKPVKKEEPKK
ECCCCCCCCCCCCC
>Mature Secondary Structure
MKLKIFALALITLFSMNSAALAISLVDADWLKKNMNNKNVKIMDVADKPNAFDKEHIPGA
CCEEHHHHHHHHHHCCCCCEEEEEEECHHHHHHCCCCCCEEEEEECCCCCCCCHHCCCCC
IQVKRYLDLSNTDRYPATAYPTKLQFEKFLSESGIENGSVIVAYDDKSSLFATRLLFLLE
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHH
MFGHDPAKLKLLNGGIVNWKKLGNKVTDDVTKIKKSKYKIAKTKTDMTVTWSDIYRDVAQ
HHCCCCCEEEEECCCCEEHHHHCCHHHHHHHHHHHHHEEEEECCCCCEEEHHHHHHHHHC
GAKPEVLLLDSRPENEFKAQNIRAIRGGYLPKAVLVTSSDANEKDSQLFKSLEDIKKLYE
CCCCCEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AKDVNGDRTIYTYCHSGDRSAHTYVILKYMLGYKNVKVYEGGWVEWANMVALPMAGQIWL
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCEEEHHEEEECCCCEEEE
WDAPKPVKKEEPKK
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2294090 [H]