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The map label for this gene is hmeA [H]

Identifier: 189424213

GI number: 189424213

Start: 1199525

End: 1200295

Strand: Reverse

Name: hmeA [H]

Synonym: Glov_1148

Alternate gene names: 189424213

Gene position: 1200295-1199525 (Counterclockwise)

Preceding gene: 189424214

Following gene: 189424212

Centisome position: 30.64

GC content: 57.33

Gene sequence:

>771_bases
ATGGCAAAACAATACGGAATGGTCATAGACCTGCAAAAATGTGTCGGCTGCGGCGCCTGCGCCCTGGGCTGCAAGACCGA
GAACAACACTCAGGCCCGCACAAACGGCCAGACCTTTAACTGGGCCGACTTCATCTACAAAGAAGAGGGGAAATTCCCCA
AGGTAAACTTTTCAGCGATTCCGGTGCTCTGCAACCACTGCAGTGACGCCCCCTGCGTCAAGGCCTGCCCGGTGAAACCC
AAGGCGATGTTCAAGACGCCGGACGGCATCACCATGCATAATGATGAGCGTTGTATCGGCTGTCGCCGCTGCCAGAAGGC
CTGTCCCTACAGCGCCATGGATGTGGCAAAGGAAAAGGCCGAGTATTCGGTGATCAGCGCCAACAGTGGCACTGAAGCCC
CGCACAAGGCCTCCCGTGATACTACCGAACTGATCAAGGGCTGCACCGCCTCCGGTGCGGAGACCGCCAAGGCTGCCGGC
GGCGTCATCCCCAATCACAACAAGTACAGCCACCCTGATTACGCCAGCATCCGCAAGCAGGGTGTGGTTGAAAAGTGTAT
TTTCTGTGCCCACCGCGTGGCCAAAGGCGAGCAGCCCACCTGCGTGACGGTCTGCCCTTCCAGGGCACGTGTCTTCGGCG
ACCTGAATGATGCCTCATCAGAACCGGCAAAACTGCTCAAGAAGTACAAGGCCGTACGCCTGAAGGAAGATAAGAAGACC
AGGCCCAATGTGGCCTATATCCGTTCCTTCAAGGCAACTGCCAAGATCTGA

Upstream 100 bases:

>100_bases
ATGCCGTCAGACTTTGACCGTTTGAGCGGCGCAACGGCACGTAACGGCGGTTTCACCGGCGTCAAGATCGAGAAAGCTTA
GGAAAGGGGGGATCTGATAG

Downstream 100 bases:

>100_bases
CCATACTGTTAAGCTGTTAACTGTACTAACGGGATGGGACGAAAGTCCCATCCCGCTTTGCTAAGGAGCCAACCATGCCG
GAACAAACCGCCACCACCTG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAKQYGMVIDLQKCVGCGACALGCKTENNTQARTNGQTFNWADFIYKEEGKFPKVNFSAIPVLCNHCSDAPCVKACPVKP
KAMFKTPDGITMHNDERCIGCRRCQKACPYSAMDVAKEKAEYSVISANSGTEAPHKASRDTTELIKGCTASGAETAKAAG
GVIPNHNKYSHPDYASIRKQGVVEKCIFCAHRVAKGEQPTCVTVCPSRARVFGDLNDASSEPAKLLKKYKAVRLKEDKKT
RPNVAYIRSFKATAKI

Sequences:

>Translated_256_residues
MAKQYGMVIDLQKCVGCGACALGCKTENNTQARTNGQTFNWADFIYKEEGKFPKVNFSAIPVLCNHCSDAPCVKACPVKP
KAMFKTPDGITMHNDERCIGCRRCQKACPYSAMDVAKEKAEYSVISANSGTEAPHKASRDTTELIKGCTASGAETAKAAG
GVIPNHNKYSHPDYASIRKQGVVEKCIFCAHRVAKGEQPTCVTVCPSRARVFGDLNDASSEPAKLLKKYKAVRLKEDKKT
RPNVAYIRSFKATAKI
>Mature_255_residues
AKQYGMVIDLQKCVGCGACALGCKTENNTQARTNGQTFNWADFIYKEEGKFPKVNFSAIPVLCNHCSDAPCVKACPVKPK
AMFKTPDGITMHNDERCIGCRRCQKACPYSAMDVAKEKAEYSVISANSGTEAPHKASRDTTELIKGCTASGAETAKAAGG
VIPNHNKYSHPDYASIRKQGVVEKCIFCAHRVAKGEQPTCVTVCPSRARVFGDLNDASSEPAKLLKKYKAVRLKEDKKTR
PNVAYIRSFKATAKI

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=126, Percent_Identity=34.9206349206349, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787122, Length=115, Percent_Identity=37.3913043478261, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI2367345, Length=108, Percent_Identity=36.1111111111111, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=132, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082179, Length=109, Percent_Identity=28.4403669724771, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27881; Mature: 27750

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
6.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQYGMVIDLQKCVGCGACALGCKTENNTQARTNGQTFNWADFIYKEEGKFPKVNFSAI
CCCCCCCEEEHHHHHCCCHHEECCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCHHH
PVLCNHCSDAPCVKACPVKPKAMFKTPDGITMHNDERCIGCRRCQKACPYSAMDVAKEKA
HHHHHCCCCCCCCCCCCCCCCCEEECCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
EYSVISANSGTEAPHKASRDTTELIKGCTASGAETAKAAGGVIPNHNKYSHPDYASIRKQ
CEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
GVVEKCIFCAHRVAKGEQPTCVTVCPSRARVFGDLNDASSEPAKLLKKYKAVRLKEDKKT
HHHHHHHHHHHHHHCCCCCCEEEECCCHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
RPNVAYIRSFKATAKI
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQYGMVIDLQKCVGCGACALGCKTENNTQARTNGQTFNWADFIYKEEGKFPKVNFSAI
CCCCCCEEEHHHHHCCCHHEECCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCHHH
PVLCNHCSDAPCVKACPVKPKAMFKTPDGITMHNDERCIGCRRCQKACPYSAMDVAKEKA
HHHHHCCCCCCCCCCCCCCCCCEEECCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
EYSVISANSGTEAPHKASRDTTELIKGCTASGAETAKAAGGVIPNHNKYSHPDYASIRKQ
CEEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
GVVEKCIFCAHRVAKGEQPTCVTVCPSRARVFGDLNDASSEPAKLLKKYKAVRLKEDKKT
HHHHHHHHHHHHHHCCCCCCEEEECCCHHHEEECCCCCCCCHHHHHHHHHHHHCCCCCCC
RPNVAYIRSFKATAKI
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]