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The map label for this gene is pcm

Identifier: 189424152

GI number: 189424152

Start: 1134114

End: 1134767

Strand: Reverse

Name: pcm

Synonym: Glov_1086

Alternate gene names: 189424152

Gene position: 1134767-1134114 (Counterclockwise)

Preceding gene: 189424153

Following gene: 189424151

Centisome position: 28.96

GC content: 60.24

Gene sequence:

>654_bases
TTGGTAAATTTTGACATCGCCCGCAAACGAATGGTGCAGGAACAGATCGTTGGCCGCGGCATTACCACCCCTCGTCTGCT
TGAAGTCTTTCAGAAGGTTCCCCGCCACCTGTTCGTGCAGGAAGCCATGGCGGTTCAGGCCTACAGTGACGGCCCGTTGC
CGATCGGCGAAAAGCAAACCATTTCACAACCCTACATTGTCGCACTGATGACCGATCTGCTGGAGCTGACCGGAAATGAT
CATGTACTTGAGATCGGTACCGGTTCCGGCTATCAGACTGCCATCCTGGCAAGCCTGGTCAGGCGGGTCTGGACCATTGA
GCGGATCCGCCCCCTGGCCATGCAGGCCCGCAAGGTGCTTGACACGCTGCACCTGCTGAACGTCAACATCAAAGTGGGTG
ACGGCACCCTTGGGTGGCCGGAGGAGGGCCCCTTTGACGCCATTCTGGTCACTGCCGGTGCCCCCGCAGTTCCGGAAACC
CTGGCGGCCCAACTGGCGCCGGGAGGCCGGCTGGTCATACCGGTCGGTGACGAATCGAACCAGACCCTGCTTCGGATTCG
CAAGGCAGCCGACGGAACCCTGACCCAGGAAACCGGTATCGGCTGCCGCTTTGTCCCGTTGATTGGCCAACAGGGCTGGC
AGCTCAACAGTTGA

Upstream 100 bases:

>100_bases
GCTCTGGAGGCCCTGAAGCAATGGCAACTCGCCTGATGCTGGACATACGCGGGCTACTCTGGTATCGTGCCGCCGATTAT
CTTCCGCCGGGGAGCCTGTT

Downstream 100 bases:

>100_bases
GGAGGTTTCATGTCGCTTGACACGCCCCGCCAGGAGCCGTTTCTGGATGCACCAGGTCTGGATGACGAACAGGAACCGCC
GGTTGACTTTGTTGCCGCCA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MVNFDIARKRMVQEQIVGRGITTPRLLEVFQKVPRHLFVQEAMAVQAYSDGPLPIGEKQTISQPYIVALMTDLLELTGND
HVLEIGTGSGYQTAILASLVRRVWTIERIRPLAMQARKVLDTLHLLNVNIKVGDGTLGWPEEGPFDAILVTAGAPAVPET
LAAQLAPGGRLVIPVGDESNQTLLRIRKAADGTLTQETGIGCRFVPLIGQQGWQLNS

Sequences:

>Translated_217_residues
MVNFDIARKRMVQEQIVGRGITTPRLLEVFQKVPRHLFVQEAMAVQAYSDGPLPIGEKQTISQPYIVALMTDLLELTGND
HVLEIGTGSGYQTAILASLVRRVWTIERIRPLAMQARKVLDTLHLLNVNIKVGDGTLGWPEEGPFDAILVTAGAPAVPET
LAAQLAPGGRLVIPVGDESNQTLLRIRKAADGTLTQETGIGCRFVPLIGQQGWQLNS
>Mature_217_residues
MVNFDIARKRMVQEQIVGRGITTPRLLEVFQKVPRHLFVQEAMAVQAYSDGPLPIGEKQTISQPYIVALMTDLLELTGND
HVLEIGTGSGYQTAILASLVRRVWTIERIRPLAMQARKVLDTLHLLNVNIKVGDGTLGWPEEGPFDAILVTAGAPAVPET
LAAQLAPGGRLVIPVGDESNQTLLRIRKAADGTLTQETGIGCRFVPLIGQQGWQLNS

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=204, Percent_Identity=36.7647058823529, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1789100, Length=195, Percent_Identity=51.2820512820513, Blast_Score=179, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71983477, Length=210, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193207222, Length=208, Percent_Identity=32.2115384615385, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17981723, Length=179, Percent_Identity=35.7541899441341, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_GEOLS (B3E6I4)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951329.1
- ProteinModelPortal:   B3E6I4
- SMR:   B3E6I4
- GeneID:   6368170
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_1086
- HOGENOM:   HBG699907
- OMA:   MGAKEDL
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23637; Mature: 23637

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNFDIARKRMVQEQIVGRGITTPRLLEVFQKVPRHLFVQEAMAVQAYSDGPLPIGEKQT
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ISQPYIVALMTDLLELTGNDHVLEIGTGSGYQTAILASLVRRVWTIERIRPLAMQARKVL
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTLHLLNVNIKVGDGTLGWPEEGPFDAILVTAGAPAVPETLAAQLAPGGRLVIPVGDESN
HHHHHEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCC
QTLLRIRKAADGTLTQETGIGCRFVPLIGQQGWQLNS
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MVNFDIARKRMVQEQIVGRGITTPRLLEVFQKVPRHLFVQEAMAVQAYSDGPLPIGEKQT
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ISQPYIVALMTDLLELTGNDHVLEIGTGSGYQTAILASLVRRVWTIERIRPLAMQARKVL
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTLHLLNVNIKVGDGTLGWPEEGPFDAILVTAGAPAVPETLAAQLAPGGRLVIPVGDESN
HHHHHEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCC
QTLLRIRKAADGTLTQETGIGCRFVPLIGQQGWQLNS
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA