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The map label for this gene is sigA [H]

Identifier: 189424151

GI number: 189424151

Start: 1133142

End: 1134104

Strand: Reverse

Name: sigA [H]

Synonym: Glov_1085

Alternate gene names: 189424151

Gene position: 1134104-1133142 (Counterclockwise)

Preceding gene: 189424152

Following gene: 189424150

Centisome position: 28.95

GC content: 55.04

Gene sequence:

>963_bases
ATGTCGCTTGACACGCCCCGCCAGGAGCCGTTTCTGGATGCACCAGGTCTGGATGACGAACAGGAACCGCCGGTTGACTT
TGTTGCCGCCATTGAAGCGGAAGAAGACACCACACTTGAAGTTGACGACCTGCCGGCCCTGGTGGAACAGATTCCCCTTG
ATGATCCGGTCAAGATCTACCTGCGGGATATCCAGCGTACCCCCTTGTTGAACGCAGAAGCCGAGAAGGAACTGGCCCGT
CGGGTTGAACAGGGAGACAAAGCGGCCCGCAACCAGATGATTGAGGCCAACCTGCGGCTGGTGGTCAAGATTGCCAAGCG
CTACCTGAATCGCGGTCTGCCGTTCCTTGACCTGATTGAAGAAGGCAACCTGGGCCTGATGAAGGCGGTCGAACGGTTCT
CCCTAGAAAAGGAATGCCGCTTTTCAACCTACGCAACCTGGTGGATCCGTCAATCAATCGAACGCGCCCTGGTAAATCAG
TCTAGAACTATCAGGCTTCCGGTCCATGTCTCTGACGATATAAGTCGCATGCTTAAGGTATCGCGCCAGCTTTCGCAGGA
GCTGCACAGAGAACCGTCTGTTCAGGAACTGGCGGATGCCATGCAGATCAAGCCGCTGCATATCCGCAAACTGATGATCC
TGCTGCGGCGGACCTGTTCCATGGATGTGCCGATTGGTGAGGGCAACGACTTTTTCCTGGTGGACACCATCGAAGACACC
AATGCGGTTTCACCCGCCGATCTGCTTGAAAACATTGATCGCTATGAGCTGATAGCCGAGCATTTTGATCAGCTGAGCGA
GAACGAACGCACCATTCTGGGGCTCCGCTTTGGCCTTGATGACCGGGACCCCCAGACTCTGGACACCATCGGCCAGAGTT
TCGGCGTTACCCGTGAAAGGATTCGCCAGATCGAGGCAAAATCACTCGAAAAACTAAAGGCCCTGGCCAAATCATCGAAA
TAG

Upstream 100 bases:

>100_bases
GGCAGCCGACGGAACCCTGACCCAGGAAACCGGTATCGGCTGCCGCTTTGTCCCGTTGATTGGCCAACAGGGCTGGCAGC
TCAACAGTTGAGGAGGTTTC

Downstream 100 bases:

>100_bases
AAACAAGGAGATTACTCCCATGGAAGATCTTAAACTTACCATTCGCGACATCCCTGATTTCCCCAAAAAGGGGATCATTT
TCAAAGACATCACCACCCTG

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSLDTPRQEPFLDAPGLDDEQEPPVDFVAAIEAEEDTTLEVDDLPALVEQIPLDDPVKIYLRDIQRTPLLNAEAEKELAR
RVEQGDKAARNQMIEANLRLVVKIAKRYLNRGLPFLDLIEEGNLGLMKAVERFSLEKECRFSTYATWWIRQSIERALVNQ
SRTIRLPVHVSDDISRMLKVSRQLSQELHREPSVQELADAMQIKPLHIRKLMILLRRTCSMDVPIGEGNDFFLVDTIEDT
NAVSPADLLENIDRYELIAEHFDQLSENERTILGLRFGLDDRDPQTLDTIGQSFGVTRERIRQIEAKSLEKLKALAKSSK

Sequences:

>Translated_320_residues
MSLDTPRQEPFLDAPGLDDEQEPPVDFVAAIEAEEDTTLEVDDLPALVEQIPLDDPVKIYLRDIQRTPLLNAEAEKELAR
RVEQGDKAARNQMIEANLRLVVKIAKRYLNRGLPFLDLIEEGNLGLMKAVERFSLEKECRFSTYATWWIRQSIERALVNQ
SRTIRLPVHVSDDISRMLKVSRQLSQELHREPSVQELADAMQIKPLHIRKLMILLRRTCSMDVPIGEGNDFFLVDTIEDT
NAVSPADLLENIDRYELIAEHFDQLSENERTILGLRFGLDDRDPQTLDTIGQSFGVTRERIRQIEAKSLEKLKALAKSSK
>Mature_319_residues
SLDTPRQEPFLDAPGLDDEQEPPVDFVAAIEAEEDTTLEVDDLPALVEQIPLDDPVKIYLRDIQRTPLLNAEAEKELARR
VEQGDKAARNQMIEANLRLVVKIAKRYLNRGLPFLDLIEEGNLGLMKAVERFSLEKECRFSTYATWWIRQSIERALVNQS
RTIRLPVHVSDDISRMLKVSRQLSQELHREPSVQELADAMQIKPLHIRKLMILLRRTCSMDVPIGEGNDFFLVDTIEDTN
AVSPADLLENIDRYELIAEHFDQLSENERTILGLRFGLDDRDPQTLDTIGQSFGVTRERIRQIEAKSLEKLKALAKSSK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=303, Percent_Identity=43.2343234323432, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1789448, Length=261, Percent_Identity=45.5938697318008, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1789871, Length=278, Percent_Identity=28.0575539568345, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36653; Mature: 36522

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00715 SIGMA70_1 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDTPRQEPFLDAPGLDDEQEPPVDFVAAIEAEEDTTLEVDDLPALVEQIPLDDPVKIY
CCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEECCCCCEEEHHHHHHHHHHCCCCCHHHHH
LRDIQRTPLLNAEAEKELARRVEQGDKAARNQMIEANLRLVVKIAKRYLNRGLPFLDLIE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EGNLGLMKAVERFSLEKECRFSTYATWWIRQSIERALVNQSRTIRLPVHVSDDISRMLKV
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
SRQLSQELHREPSVQELADAMQIKPLHIRKLMILLRRTCSMDVPIGEGNDFFLVDTIEDT
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
NAVSPADLLENIDRYELIAEHFDQLSENERTILGLRFGLDDRDPQTLDTIGQSFGVTRER
CCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHH
IRQIEAKSLEKLKALAKSSK
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLDTPRQEPFLDAPGLDDEQEPPVDFVAAIEAEEDTTLEVDDLPALVEQIPLDDPVKIY
CCCCCCCCCCCCCCCCCCCCCCCHHHHEEEECCCCCEEEHHHHHHHHHHCCCCCHHHHH
LRDIQRTPLLNAEAEKELARRVEQGDKAARNQMIEANLRLVVKIAKRYLNRGLPFLDLIE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EGNLGLMKAVERFSLEKECRFSTYATWWIRQSIERALVNQSRTIRLPVHVSDDISRMLKV
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
SRQLSQELHREPSVQELADAMQIKPLHIRKLMILLRRTCSMDVPIGEGNDFFLVDTIEDT
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
NAVSPADLLENIDRYELIAEHFDQLSENERTILGLRFGLDDRDPQTLDTIGQSFGVTRER
CCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCHHHHH
IRQIEAKSLEKLKALAKSSK
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]