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The map label for this gene is slt [H]

Identifier: 189424131

GI number: 189424131

Start: 1106481

End: 1108577

Strand: Reverse

Name: slt [H]

Synonym: Glov_1065

Alternate gene names: 189424131

Gene position: 1108577-1106481 (Counterclockwise)

Preceding gene: 189424136

Following gene: 189424130

Centisome position: 28.3

GC content: 60.47

Gene sequence:

>2097_bases
ATGACCTACAAACTGCATCGTCTTCTGGCAGGCTCCCTGACCATGCTGGCCGCCACGGCAACATTGACCTGCGCCGGTTC
CACCATAACCGCCAACGAACAACTCCGTAAGGCAGCCGGCTTGCTACGCAACAACCAGAACCAGGAGGCGCTGCAGGCCG
CCGCCAACGCACCTGCCGACGGCCCCCGCAACCTGCTGGCCGGTGAAGCAGCCCTACGCCTGAAAAGGTATGATGAGGCG
ATCCGCTATCTGGCTGATGCCGAGCGCAGCTATCCGCTGCTGGCTGATATTGCAGCGGCCCAGAAGGCCGATGCCCTGTT
CGGCAAAAAACAGTACCGTGAGGCTGCTGCCGCAGCCACTACTGCAGCGCAGCGTTCACCGACACCGGCGGTCAGCCGCC
GGATGGAAAAGCTGGCAGCCGACGCACTGTTTGAAGCGGGTGACCTGAAGGCAGCCCTGACTGCCTATCGCCAGTTCACC
ACCCGCCACAGCCTGGGCAGAGATCATGTTGACGCCCTGTTTCAGACTGCCCTCTGCCAGGAAGGTTTGAATGAATTGAG
CGCAGCAGCCCAACTCTATCGCACCATCTATCTGCAGCATCCTGCCTCAACACCCGCCTCAAAGGTCATGGAGCGCCTGA
AGACACTGGAGAAGAAAGGCCAGAAGGATGCCGGTTCCTTTACGGCAGAAGAACAGTTCCGGCGGGGGCAGCTGTTGCTG
GCCAACAACCAGCCCAATGCAGCGGCCTGGGTCTTTGCAGGCATCCCCCGCACCAATCTGTCTGAAGAACTGCTGGCACA
GATTGAGCTGAAATCAGGCCAGGCGGCAATCAAACAGCGCCGTTACTCCCTTGCTGAACCATTCCTGAAGCGGGCTGCAG
CTGCCCGTACTGCAACAACCAGAGATGAGGCCAGGCTGACGCTGGCACGGGTGGAAGAACGGCAGGGCGGTTCAGAAAAG
GCACTGGCCAGGTTGCTGACACTGGCCTCCGAAAAGGGGGCACTGGCCGACGATGCCCTGCTGGAGGCAGCCCTGATCAA
CAAACACAACGGCCGCCACAGTGATGCATCCCAGATCCTGCAACGCCTGATCAAAGATTTTCCGTCATCCGGCCTGATCC
CCCGGGCCGGCTGGGAGCTGGCCTGGGGCCAGTATCTGGCAGGCAACCTGCCGGCTGCTGCAGAAAGTTTCAGATTGCTG
TTCAAGGATACGCTCTATCGTGAGCGGGCCCTGTACTGGTATGCCCGTAGCCAGGACCGGCAAAACAGGCCGGCAGACGC
AGAGCGTGCCTACAAACAACTGCTGGGCGAGTACCCCTACGGTTTTTACGCAGCATGGCACCGGCTGCGCACCAACAAGC
CGACCGGCTGGATGGCGTTGCCTGAAGGAATCCCCGAGCCTGCACTGCCTGAAGGCAGCCAACGGATTCAGGCACTGGCA
GCCCTGGGGATGCTGGAAGAGGCCCGCACGGAGCTGGCCGCCTTCAAGGCCAAGGGCCCGGACAAGGAGACGGCACCGGG
CATGGCGCGTCTGCAGTACCTTGCCGGTGACCTGCACGGTTCGATAGTCACCTTTCACCAGAATCGCCCGGCTGTAATTG
ACCGTTCCACCCTGCCGTTCTGGGCGCTCGGGTTCCCCCGCCCCTACGCTGAGCTGTTCAGCCGCAACAGCACGGCAAAC
GGTTTGCCTGACTCACTGGTGCTTTCGTTGGCCAAGGCAGAAAGCAGCTTTCGGGCTGATGTCAAATCCCATGCAGGGGC
GATCGGCCTGATGCAGCTGATGCCGGCAACGGCCCGGATGACTGCCGGCTACAAGGGCAAAAACTACAACCCGCTCTGGC
TGATCGAGCCGGAATACAACATCAGACTGGGCACCAAACATCTGCGGGAACTGTTGAACCAGTACCATCAGGATCAGATC
TATACCCTGGCCGCCTACAACGCCGGAGCAGGGGCGGTCAACCGTTGGCGTAAATCCTTTGGCGACCTGCCCAGGGACGA
GTTTGTGGAAAACATCCCTTATCAGGAGACCCGTGACTACGTGAAAAAGATCGTGGCCTACATGGCGGTCTACCGCTCAC
TCTACCGGATTCAATAG

Upstream 100 bases:

>100_bases
AAACCCCGGTTCACACCATTGACCTGACAGCCGCATGATGTTACTGAAAAGAGACCAGCTCAACAAACCAGATTTTCCCC
CACAGGACTACCGTACCGCT

Downstream 100 bases:

>100_bases
GAGAAAGAGATGACCAAACTCGCCCTGTTCAGTATTGCGCTGATGCTTCTGATTTCCACTCTGAGCCAGGCAGGACCGGC
AGAATCAATGCAACAGTTGA

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MTYKLHRLLAGSLTMLAATATLTCAGSTITANEQLRKAAGLLRNNQNQEALQAAANAPADGPRNLLAGEAALRLKRYDEA
IRYLADAERSYPLLADIAAAQKADALFGKKQYREAAAAATTAAQRSPTPAVSRRMEKLAADALFEAGDLKAALTAYRQFT
TRHSLGRDHVDALFQTALCQEGLNELSAAAQLYRTIYLQHPASTPASKVMERLKTLEKKGQKDAGSFTAEEQFRRGQLLL
ANNQPNAAAWVFAGIPRTNLSEELLAQIELKSGQAAIKQRRYSLAEPFLKRAAAARTATTRDEARLTLARVEERQGGSEK
ALARLLTLASEKGALADDALLEAALINKHNGRHSDASQILQRLIKDFPSSGLIPRAGWELAWGQYLAGNLPAAAESFRLL
FKDTLYRERALYWYARSQDRQNRPADAERAYKQLLGEYPYGFYAAWHRLRTNKPTGWMALPEGIPEPALPEGSQRIQALA
ALGMLEEARTELAAFKAKGPDKETAPGMARLQYLAGDLHGSIVTFHQNRPAVIDRSTLPFWALGFPRPYAELFSRNSTAN
GLPDSLVLSLAKAESSFRADVKSHAGAIGLMQLMPATARMTAGYKGKNYNPLWLIEPEYNIRLGTKHLRELLNQYHQDQI
YTLAAYNAGAGAVNRWRKSFGDLPRDEFVENIPYQETRDYVKKIVAYMAVYRSLYRIQ

Sequences:

>Translated_698_residues
MTYKLHRLLAGSLTMLAATATLTCAGSTITANEQLRKAAGLLRNNQNQEALQAAANAPADGPRNLLAGEAALRLKRYDEA
IRYLADAERSYPLLADIAAAQKADALFGKKQYREAAAAATTAAQRSPTPAVSRRMEKLAADALFEAGDLKAALTAYRQFT
TRHSLGRDHVDALFQTALCQEGLNELSAAAQLYRTIYLQHPASTPASKVMERLKTLEKKGQKDAGSFTAEEQFRRGQLLL
ANNQPNAAAWVFAGIPRTNLSEELLAQIELKSGQAAIKQRRYSLAEPFLKRAAAARTATTRDEARLTLARVEERQGGSEK
ALARLLTLASEKGALADDALLEAALINKHNGRHSDASQILQRLIKDFPSSGLIPRAGWELAWGQYLAGNLPAAAESFRLL
FKDTLYRERALYWYARSQDRQNRPADAERAYKQLLGEYPYGFYAAWHRLRTNKPTGWMALPEGIPEPALPEGSQRIQALA
ALGMLEEARTELAAFKAKGPDKETAPGMARLQYLAGDLHGSIVTFHQNRPAVIDRSTLPFWALGFPRPYAELFSRNSTAN
GLPDSLVLSLAKAESSFRADVKSHAGAIGLMQLMPATARMTAGYKGKNYNPLWLIEPEYNIRLGTKHLRELLNQYHQDQI
YTLAAYNAGAGAVNRWRKSFGDLPRDEFVENIPYQETRDYVKKIVAYMAVYRSLYRIQ
>Mature_697_residues
TYKLHRLLAGSLTMLAATATLTCAGSTITANEQLRKAAGLLRNNQNQEALQAAANAPADGPRNLLAGEAALRLKRYDEAI
RYLADAERSYPLLADIAAAQKADALFGKKQYREAAAAATTAAQRSPTPAVSRRMEKLAADALFEAGDLKAALTAYRQFTT
RHSLGRDHVDALFQTALCQEGLNELSAAAQLYRTIYLQHPASTPASKVMERLKTLEKKGQKDAGSFTAEEQFRRGQLLLA
NNQPNAAAWVFAGIPRTNLSEELLAQIELKSGQAAIKQRRYSLAEPFLKRAAAARTATTRDEARLTLARVEERQGGSEKA
LARLLTLASEKGALADDALLEAALINKHNGRHSDASQILQRLIKDFPSSGLIPRAGWELAWGQYLAGNLPAAAESFRLLF
KDTLYRERALYWYARSQDRQNRPADAERAYKQLLGEYPYGFYAAWHRLRTNKPTGWMALPEGIPEPALPEGSQRIQALAA
LGMLEEARTELAAFKAKGPDKETAPGMARLQYLAGDLHGSIVTFHQNRPAVIDRSTLPFWALGFPRPYAELFSRNSTANG
LPDSLVLSLAKAESSFRADVKSHAGAIGLMQLMPATARMTAGYKGKNYNPLWLIEPEYNIRLGTKHLRELLNQYHQDQIY
TLAAYNAGAGAVNRWRKSFGDLPRDEFVENIPYQETRDYVKKIVAYMAVYRSLYRIQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=155, Percent_Identity=41.9354838709677, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77252; Mature: 77121

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKLHRLLAGSLTMLAATATLTCAGSTITANEQLRKAAGLLRNNQNQEALQAAANAPAD
CCHHHHHHHHHHHHHHHHHHHHEECCCEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
GPRNLLAGEAALRLKRYDEAIRYLADAERSYPLLADIAAAQKADALFGKKQYREAAAAAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAAQRSPTPAVSRRMEKLAADALFEAGDLKAALTAYRQFTTRHSLGRDHVDALFQTALCQ
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EGLNELSAAAQLYRTIYLQHPASTPASKVMERLKTLEKKGQKDAGSFTAEEQFRRGQLLL
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEE
ANNQPNAAAWVFAGIPRTNLSEELLAQIELKSGQAAIKQRRYSLAEPFLKRAAAARTATT
ECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
RDEARLTLARVEERQGGSEKALARLLTLASEKGALADDALLEAALINKHNGRHSDASQIL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
QRLIKDFPSSGLIPRAGWELAWGQYLAGNLPAAAESFRLLFKDTLYRERALYWYARSQDR
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCC
QNRPADAERAYKQLLGEYPYGFYAAWHRLRTNKPTGWMALPEGIPEPALPEGSQRIQALA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHH
ALGMLEEARTELAAFKAKGPDKETAPGMARLQYLAGDLHGSIVTFHQNRPAVIDRSTLPF
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCE
WALGFPRPYAELFSRNSTANGLPDSLVLSLAKAESSFRADVKSHAGAIGLMQLMPATARM
EECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHH
TAGYKGKNYNPLWLIEPEYNIRLGTKHLRELLNQYHQDQIYTLAAYNAGAGAVNRWRKSF
HCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
GDLPRDEFVENIPYQETRDYVKKIVAYMAVYRSLYRIQ
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TYKLHRLLAGSLTMLAATATLTCAGSTITANEQLRKAAGLLRNNQNQEALQAAANAPAD
CHHHHHHHHHHHHHHHHHHHHEECCCEECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
GPRNLLAGEAALRLKRYDEAIRYLADAERSYPLLADIAAAQKADALFGKKQYREAAAAAT
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAAQRSPTPAVSRRMEKLAADALFEAGDLKAALTAYRQFTTRHSLGRDHVDALFQTALCQ
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EGLNELSAAAQLYRTIYLQHPASTPASKVMERLKTLEKKGQKDAGSFTAEEQFRRGQLLL
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEE
ANNQPNAAAWVFAGIPRTNLSEELLAQIELKSGQAAIKQRRYSLAEPFLKRAAAARTATT
ECCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
RDEARLTLARVEERQGGSEKALARLLTLASEKGALADDALLEAALINKHNGRHSDASQIL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHH
QRLIKDFPSSGLIPRAGWELAWGQYLAGNLPAAAESFRLLFKDTLYRERALYWYARSQDR
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCC
QNRPADAERAYKQLLGEYPYGFYAAWHRLRTNKPTGWMALPEGIPEPALPEGSQRIQALA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHH
ALGMLEEARTELAAFKAKGPDKETAPGMARLQYLAGDLHGSIVTFHQNRPAVIDRSTLPF
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCE
WALGFPRPYAELFSRNSTANGLPDSLVLSLAKAESSFRADVKSHAGAIGLMQLMPATARM
EECCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHH
TAGYKGKNYNPLWLIEPEYNIRLGTKHLRELLNQYHQDQIYTLAAYNAGAGAVNRWRKSF
HCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHH
GDLPRDEFVENIPYQETRDYVKKIVAYMAVYRSLYRIQ
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]