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The map label for this gene is hisF

Identifier: 189424084

GI number: 189424084

Start: 1057756

End: 1058520

Strand: Reverse

Name: hisF

Synonym: Glov_1018

Alternate gene names: 189424084

Gene position: 1058520-1057756 (Counterclockwise)

Preceding gene: 189424085

Following gene: 189424083

Centisome position: 27.02

GC content: 61.44

Gene sequence:

>765_bases
ATGCTGACCCGCCGTATCATTCCCTGTCTGGATGTGACCGGCGGCCGGGTGGTCAAGGGGGTCCAGTTTCTTGAGCTGCG
TGATGCCGGTGATCCGGTGGAAATCGCCGAGGCCTATGACCTGCAAGGGGCCGATGAGCTGACCTTTCTGGATATCACCG
CATCCAGCGATGGGCGCGACACCATGGTGGATGTGGTACGCCATACCGCAGAGCGGGTCTTTATGCCGCTGACCGTGGGC
GGCGGTATCAGGGTGGTGGAGGATATCCGCCGGATGCTGAACGCCGGAGCAGACAAAGTTTCGATCAACACCGCTGCGGT
CAGCCGGCCTGAGTTTGTGAATGAGGCTGCCGAGCGGTTCGGTTCCCAGTGTACCGTCGTGGCCATTGATGCCCGCCAGG
TACCGGGTGAGCAGCGCTGGGAGGTCTACACCCACGGCGGCCGCAAACCGACCGGAATCGATGCCGTTGAATGGGCAGTC
AGGATGGAGTCTTACGGTGCCGGTGAGATCCTGCTGACCAGTATGGACCGTGACGGCACCAAGGACGGCTATGACCTGGC
CCTGACCCGTGCCGTGGTCGATGCCGTTTCAATACCGGTGATCGCCTCAGGCGGGGTCGGCAACCTTGAACATCTCTATG
ACGGCTTCACCACTGCCGGGGCCTCAGCCTGCCTGGCAGCCTCAATCTTCCACTTCCGGCAGCATACGGTACAGGAAGCC
AAGCAGTATCTCCAAGGACGGGGCGTCCCGATCAGGATGGTTTAA

Upstream 100 bases:

>100_bases
TTGAAGCCAGCGGTGTAACCGGCGCCATTACCGGCAAGGCGATCTACACCGGCGCCATCAACCTGCGTGAGGCGATTGAC
CTGACCAAGCGGGGCTGCCA

Downstream 100 bases:

>100_bases
GATGAACAGCAACCAGCATATTCTGGATGAGTTGTATCAGGTGATTCTCTCCCGCAAGGGGGCCTCGCCGGACAGTTCCT
ATACCGCCTCCCTGCTGCAC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLTRRIIPCLDVTGGRVVKGVQFLELRDAGDPVEIAEAYDLQGADELTFLDITASSDGRDTMVDVVRHTAERVFMPLTVG
GGIRVVEDIRRMLNAGADKVSINTAAVSRPEFVNEAAERFGSQCTVVAIDARQVPGEQRWEVYTHGGRKPTGIDAVEWAV
RMESYGAGEILLTSMDRDGTKDGYDLALTRAVVDAVSIPVIASGGVGNLEHLYDGFTTAGASACLAASIFHFRQHTVQEA
KQYLQGRGVPIRMV

Sequences:

>Translated_254_residues
MLTRRIIPCLDVTGGRVVKGVQFLELRDAGDPVEIAEAYDLQGADELTFLDITASSDGRDTMVDVVRHTAERVFMPLTVG
GGIRVVEDIRRMLNAGADKVSINTAAVSRPEFVNEAAERFGSQCTVVAIDARQVPGEQRWEVYTHGGRKPTGIDAVEWAV
RMESYGAGEILLTSMDRDGTKDGYDLALTRAVVDAVSIPVIASGGVGNLEHLYDGFTTAGASACLAASIFHFRQHTVQEA
KQYLQGRGVPIRMV
>Mature_254_residues
MLTRRIIPCLDVTGGRVVKGVQFLELRDAGDPVEIAEAYDLQGADELTFLDITASSDGRDTMVDVVRHTAERVFMPLTVG
GGIRVVEDIRRMLNAGADKVSINTAAVSRPEFVNEAAERFGSQCTVVAIDARQVPGEQRWEVYTHGGRKPTGIDAVEWAV
RMESYGAGEILLTSMDRDGTKDGYDLALTRAVVDAVSIPVIASGGVGNLEHLYDGFTTAGASACLAASIFHFRQHTVQEA
KQYLQGRGVPIRMV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.1906614785992, Blast_Score=206, Evalue=9e-55,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=30.8056872037915, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=37.2611464968153, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_GEOLS (B3E617)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951261.1
- ProteinModelPortal:   B3E617
- SMR:   B3E617
- GeneID:   6369137
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_1018
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27483; Mature: 27483

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRIIPCLDVTGGRVVKGVQFLELRDAGDPVEIAEAYDLQGADELTFLDITASSDGRD
CCCCCCCCEECCCCCHHHHCEEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEECCCCHH
TMVDVVRHTAERVFMPLTVGGGIRVVEDIRRMLNAGADKVSINTAAVSRPEFVNEAAERF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHH
GSQCTVVAIDARQVPGEQRWEVYTHGGRKPTGIDAVEWAVRMESYGAGEILLTSMDRDGT
CCCEEEEEEEHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCC
KDGYDLALTRAVVDAVSIPVIASGGVGNLEHLYDGFTTAGASACLAASIFHFRQHTVQEA
CCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
KQYLQGRGVPIRMV
HHHHHCCCCEEEEC
>Mature Secondary Structure
MLTRRIIPCLDVTGGRVVKGVQFLELRDAGDPVEIAEAYDLQGADELTFLDITASSDGRD
CCCCCCCCEECCCCCHHHHCEEEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEECCCCHH
TMVDVVRHTAERVFMPLTVGGGIRVVEDIRRMLNAGADKVSINTAAVSRPEFVNEAAERF
HHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHH
GSQCTVVAIDARQVPGEQRWEVYTHGGRKPTGIDAVEWAVRMESYGAGEILLTSMDRDGT
CCCEEEEEEEHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCC
KDGYDLALTRAVVDAVSIPVIASGGVGNLEHLYDGFTTAGASACLAASIFHFRQHTVQEA
CCCHHHHHHHHHHHHHHCCEEECCCCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHH
KQYLQGRGVPIRMV
HHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA