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The map label for this gene is hisA

Identifier: 189424085

GI number: 189424085

Start: 1058514

End: 1059251

Strand: Reverse

Name: hisA

Synonym: Glov_1019

Alternate gene names: 189424085

Gene position: 1059251-1058514 (Counterclockwise)

Preceding gene: 189424086

Following gene: 189424084

Centisome position: 27.04

GC content: 59.21

Gene sequence:

>738_bases
ATGATCGTCATACCTGCCATAGACCTGAAAGAAGGTAACTGCGTCCGCCTGGAACAAGGCGAGATGAACCGCGACACCGT
ATTCTCCGACAATCCTGCCGAGCAGGCCCTGAAGTGGCAGGAGGCCGGCGCAGAACTGCTGCACCTGGTGGATCTGGACG
GTGCCTTTGCCGGCATCCCCAAAAACAAGGCTGCCATTGAGGCAATTATCAAGGCGATCACCATTCCTGCCCAACTGGGT
GGCGGCATCCGTGATATCGCCACCATTGAGGCCTACCTGTCACTGGGGCTTTCCCGGGTGATCATCGGCACCGCTGCCCA
GCGCAACCCGGAACTGGTGATCGAGGCCTGCCAGAAGTTTCCCGGCCGGATTGTAGTCGGCATTGATGCCAAGAACGGCA
TGGTGGCGGTGCAGGGCTGGGCTGAACTGACTGACATCAGCGCGGTTGATCTGGCAAAGAAGTTTGAAGATTGTGGTGTT
GCCGCCATCATCTACACCGACATCAGCCGGGACGGCATGATGGGTGGCCCCAACCTTGAGGCGACCAGGGCGCTGGCAGA
GGCGATCTCAATCCCGGTGATCGCTTCCGGCGGTGTCTCTTCACTGAAGGATATCGAAAACCTGATGGCCATTGAAGCCA
GCGGTGTAACCGGCGCCATTACCGGCAAGGCGATCTACACCGGCGCCATCAACCTGCGTGAGGCGATTGACCTGACCAAG
CGGGGCTGCCAATGCTGA

Upstream 100 bases:

>100_bases
AAGTATCACTAAGAAAACTACAAAAAGCAGTCAACTTGATTTTAGCTTTACGCCTTACTTGGTGTACCTTCGTGTCCTTC
GTGGATAAAAGGAACTGATA

Downstream 100 bases:

>100_bases
CCCGCCGTATCATTCCCTGTCTGGATGTGACCGGCGGCCGGGTGGTCAAGGGGGTCCAGTTTCTTGAGCTGCGTGATGCC
GGTGATCCGGTGGAAATCGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIVIPAIDLKEGNCVRLEQGEMNRDTVFSDNPAEQALKWQEAGAELLHLVDLDGAFAGIPKNKAAIEAIIKAITIPAQLG
GGIRDIATIEAYLSLGLSRVIIGTAAQRNPELVIEACQKFPGRIVVGIDAKNGMVAVQGWAELTDISAVDLAKKFEDCGV
AAIIYTDISRDGMMGGPNLEATRALAEAISIPVIASGGVSSLKDIENLMAIEASGVTGAITGKAIYTGAINLREAIDLTK
RGCQC

Sequences:

>Translated_245_residues
MIVIPAIDLKEGNCVRLEQGEMNRDTVFSDNPAEQALKWQEAGAELLHLVDLDGAFAGIPKNKAAIEAIIKAITIPAQLG
GGIRDIATIEAYLSLGLSRVIIGTAAQRNPELVIEACQKFPGRIVVGIDAKNGMVAVQGWAELTDISAVDLAKKFEDCGV
AAIIYTDISRDGMMGGPNLEATRALAEAISIPVIASGGVSSLKDIENLMAIEASGVTGAITGKAIYTGAINLREAIDLTK
RGCQC
>Mature_245_residues
MIVIPAIDLKEGNCVRLEQGEMNRDTVFSDNPAEQALKWQEAGAELLHLVDLDGAFAGIPKNKAAIEAIIKAITIPAQLG
GGIRDIATIEAYLSLGLSRVIIGTAAQRNPELVIEACQKFPGRIVVGIDAKNGMVAVQGWAELTDISAVDLAKKFEDCGV
AAIIYTDISRDGMMGGPNLEATRALAEAISIPVIASGGVSSLKDIENLMAIEASGVTGAITGKAIYTGAINLREAIDLTK
RGCQC

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=31.8181818181818, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=25.5144032921811, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_GEOLS (B3E618)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001951262.1
- ProteinModelPortal:   B3E618
- SMR:   B3E618
- GeneID:   6369122
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_1019
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25686; Mature: 25686

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIPAIDLKEGNCVRLEQGEMNRDTVFSDNPAEQALKWQEAGAELLHLVDLDGAFAGIP
CEEECEEECCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHCCHHEEEEEECCCCCCCCC
KNKAAIEAIIKAITIPAQLGGGIRDIATIEAYLSLGLSRVIIGTAAQRNPELVIEACQKF
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHEEECCCCCCHHHHHHHHHCC
PGRIVVGIDAKNGMVAVQGWAELTDISAVDLAKKFEDCGVAAIIYTDISRDGMMGGPNLE
CCEEEEEEECCCCEEEEECCHHHHCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHH
ATRALAEAISIPVIASGGVSSLKDIENLMAIEASGVTGAITGKAIYTGAINLREAIDLTK
HHHHHHHHHCCCEEECCCHHHHHHHHHHHEEECCCCEEEEECCEEEECCCHHHHHHHHHH
RGCQC
CCCCC
>Mature Secondary Structure
MIVIPAIDLKEGNCVRLEQGEMNRDTVFSDNPAEQALKWQEAGAELLHLVDLDGAFAGIP
CEEECEEECCCCCEEEEECCCCCCCCEECCCCHHHHHHHHHCCHHEEEEEECCCCCCCCC
KNKAAIEAIIKAITIPAQLGGGIRDIATIEAYLSLGLSRVIIGTAAQRNPELVIEACQKF
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHEEECCCCCCHHHHHHHHHCC
PGRIVVGIDAKNGMVAVQGWAELTDISAVDLAKKFEDCGVAAIIYTDISRDGMMGGPNLE
CCEEEEEEECCCCEEEEECCHHHHCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCHH
ATRALAEAISIPVIASGGVSSLKDIENLMAIEASGVTGAITGKAIYTGAINLREAIDLTK
HHHHHHHHHCCCEEECCCHHHHHHHHHHHEEECCCCEEEEECCEEEECCCHHHHHHHHHH
RGCQC
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA