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The map label for this gene is gspE [H]

Identifier: 189424038

GI number: 189424038

Start: 1011310

End: 1012896

Strand: Reverse

Name: gspE [H]

Synonym: Glov_0972

Alternate gene names: 189424038

Gene position: 1012896-1011310 (Counterclockwise)

Preceding gene: 189424039

Following gene: 189424037

Centisome position: 25.85

GC content: 57.97

Gene sequence:

>1587_bases
ATGCAGACCACCACGCCCTATACACGAACCCGTGTCGGCGAGTACCTGCGGGAGCAGGGACTGATCAACCAACAGAAGCT
TGACGTCGCCCTGCAGGAACAGGCCATCACCGGCGAAAAGCTGGGAGAAATCCTGATCCGGTTCGGTTTTTTTGAGCGCA
GCCACCTGGTTGAGGCATTGGCCCACTTAAACCCGGAGTCGCTGATCGGCGAGACCTCGGTACAGGTTGAGCTGCCGCCT
GAACTACTCCTGAAATACAAGTGCATGATCCTGGGGGATACCGGCAACACCCTGTACCTGGCGACCCTGCACCCTCACCC
CCGTGAGGTGCTGGAGGCGATCGGCAAGCTGCTTGACCTCTCGATGACCCTAACCCTGGTCCCGATCCGCCACACCATCA
TTACCGAACATCTGGCCACCCTGGAACGTTCGATGCATGCCACCAGACGGGGTCAGGACGACACCGACATCAACAAACAA
CTCACCGCCATCATCCATGATGCCCTGGAGGATGGCGCCTCCGACATCCATCTGGAGACATCGGAGAAATCTTTGCATGT
CCGTTGCCGAATCGATGGTATCCTGCATATGGTCAAGGCCCTGCCGGACAGCCTGTCCAACAAGATCTTCTCCCGGATCA
AGGACATGTCCGGCATGAACGTATCCGACAAGATGGTGCCCCAGGACGGCTCCTTTTCCATGAACTACCGTGGTCGCAAT
GTCGACTTCCGGGTTTCAACCATCCCATCCACCTACGGCGAAAAGGCGACCATCCGGATCCTGGACAAGGAAAAGATGAT
GATCGGGGTGCAGGAAATCGGCATCAGCGCCATCAACGACTGGCTTGAACTGGCCAGGCTGACTACCGGCCTGATCCTGG
TCTGCGGTGCCACCGGCTCCGGCAAGACCACCACGCTTTATTCGACGGTACGGCACTTGAACCTGCTGGAAAAGGCAGTC
TACTTTGTGGAAGATCCGGTTGAATACCGGGTGCCGTTTGTGACCCAGGTGCAGGTGAATCGCCGCATCAACCTTGATTT
TGCCGCCTTTGGCAGGACCGTGCTGCGCCATGACCCTGATGTGGTGGTTGTTGGCGAGGTACGGGACAGCGAGACCGCAG
AGAACGCCCTCTGGCTGGCCGATACCGGCCACCTGGTCTATGCCACCCTGCATACCAACGACATCCCCTCCACCATTCTG
CGGCTAAAAGACCTGGGGGCAGAGATCCCCAAACTCTCCTATGCGCTGCGGGGGATACTGGTGCAGAAACTGGCCCGCAA
ACTGTGCGCCCAGTGTGAGGGAGCCGGTTGTGAGGAGTGCAAACAGACCGGCTACAAGGGCAGGACGCTCCTGACCGAGT
TTGCCCGCCTGAACAGCCCCAAAGATATCTACCGCCTGATTGATCACAAAGGGGAAGATGACCTGCCCTATCAACGTTTT
ATTGACGATGCCCGCACCAAACTGACAAACCGGACAACCGACTGCGCCGAGATCCAGCGGGTATTGGGAATTCGCCTGGG
TGGCGCCTGGTGTGCTTCCTGCCGTCAGCAGACCGACTGTAACGATGCCATGGAGATCATGGATTAG

Upstream 100 bases:

>100_bases
TGAAGGTTATCACCAACGCCAGTTGCGGTTCCACCACAGATTCCATGCCGACATCCGGTACCGTGTCATTCACCTACTGG
ATCAGCCGCGTCAGCTAGCG

Downstream 100 bases:

>100_bases
CCATGCATCAGGTCACCTACTTTGACAAAGGGGTCCGCAAAGAGGCGGAATTCATCGGTTCAGCCGCCGAGATCAGAGAA
CGGCTGATCAGCGAAGGCAA

Product: type II secretion system protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MQTTTPYTRTRVGEYLREQGLINQQKLDVALQEQAITGEKLGEILIRFGFFERSHLVEALAHLNPESLIGETSVQVELPP
ELLLKYKCMILGDTGNTLYLATLHPHPREVLEAIGKLLDLSMTLTLVPIRHTIITEHLATLERSMHATRRGQDDTDINKQ
LTAIIHDALEDGASDIHLETSEKSLHVRCRIDGILHMVKALPDSLSNKIFSRIKDMSGMNVSDKMVPQDGSFSMNYRGRN
VDFRVSTIPSTYGEKATIRILDKEKMMIGVQEIGISAINDWLELARLTTGLILVCGATGSGKTTTLYSTVRHLNLLEKAV
YFVEDPVEYRVPFVTQVQVNRRINLDFAAFGRTVLRHDPDVVVVGEVRDSETAENALWLADTGHLVYATLHTNDIPSTIL
RLKDLGAEIPKLSYALRGILVQKLARKLCAQCEGAGCEECKQTGYKGRTLLTEFARLNSPKDIYRLIDHKGEDDLPYQRF
IDDARTKLTNRTTDCAEIQRVLGIRLGGAWCASCRQQTDCNDAMEIMD

Sequences:

>Translated_528_residues
MQTTTPYTRTRVGEYLREQGLINQQKLDVALQEQAITGEKLGEILIRFGFFERSHLVEALAHLNPESLIGETSVQVELPP
ELLLKYKCMILGDTGNTLYLATLHPHPREVLEAIGKLLDLSMTLTLVPIRHTIITEHLATLERSMHATRRGQDDTDINKQ
LTAIIHDALEDGASDIHLETSEKSLHVRCRIDGILHMVKALPDSLSNKIFSRIKDMSGMNVSDKMVPQDGSFSMNYRGRN
VDFRVSTIPSTYGEKATIRILDKEKMMIGVQEIGISAINDWLELARLTTGLILVCGATGSGKTTTLYSTVRHLNLLEKAV
YFVEDPVEYRVPFVTQVQVNRRINLDFAAFGRTVLRHDPDVVVVGEVRDSETAENALWLADTGHLVYATLHTNDIPSTIL
RLKDLGAEIPKLSYALRGILVQKLARKLCAQCEGAGCEECKQTGYKGRTLLTEFARLNSPKDIYRLIDHKGEDDLPYQRF
IDDARTKLTNRTTDCAEIQRVLGIRLGGAWCASCRQQTDCNDAMEIMD
>Mature_528_residues
MQTTTPYTRTRVGEYLREQGLINQQKLDVALQEQAITGEKLGEILIRFGFFERSHLVEALAHLNPESLIGETSVQVELPP
ELLLKYKCMILGDTGNTLYLATLHPHPREVLEAIGKLLDLSMTLTLVPIRHTIITEHLATLERSMHATRRGQDDTDINKQ
LTAIIHDALEDGASDIHLETSEKSLHVRCRIDGILHMVKALPDSLSNKIFSRIKDMSGMNVSDKMVPQDGSFSMNYRGRN
VDFRVSTIPSTYGEKATIRILDKEKMMIGVQEIGISAINDWLELARLTTGLILVCGATGSGKTTTLYSTVRHLNLLEKAV
YFVEDPVEYRVPFVTQVQVNRRINLDFAAFGRTVLRHDPDVVVVGEVRDSETAENALWLADTGHLVYATLHTNDIPSTIL
RLKDLGAEIPKLSYALRGILVQKLARKLCAQCEGAGCEECKQTGYKGRTLLTEFARLNSPKDIYRLIDHKGEDDLPYQRF
IDDARTKLTNRTTDCAEIQRVLGIRLGGAWCASCRQQTDCNDAMEIMD

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=374, Percent_Identity=39.0374331550802, Blast_Score=266, Evalue=4e-72,
Organism=Escherichia coli, GI1786296, Length=317, Percent_Identity=37.2239747634069, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=39.4871794871795, Blast_Score=102, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 59229; Mature: 59229

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTTPYTRTRVGEYLREQGLINQQKLDVALQEQAITGEKLGEILIRFGFFERSHLVEAL
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHH
AHLNPESLIGETSVQVELPPELLLKYKCMILGDTGNTLYLATLHPHPREVLEAIGKLLDL
HHCCHHHHCCCCEEEEECCHHHHEEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHH
SMTLTLVPIRHTIITEHLATLERSMHATRRGQDDTDINKQLTAIIHDALEDGASDIHLET
HHEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
SEKSLHVRCRIDGILHMVKALPDSLSNKIFSRIKDMSGMNVSDKMVPQDGSFSMNYRGRN
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCC
VDFRVSTIPSTYGEKATIRILDKEKMMIGVQEIGISAINDWLELARLTTGLILVCGATGS
EEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKTTTLYSTVRHLNLLEKAVYFVEDPVEYRVPFVTQVQVNRRINLDFAAFGRTVLRHDPD
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCEECCCHHHHCCHHHHCCCC
VVVVGEVRDSETAENALWLADTGHLVYATLHTNDIPSTILRLKDLGAEIPKLSYALRGIL
EEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHH
VQKLARKLCAQCEGAGCEECKQTGYKGRTLLTEFARLNSPKDIYRLIDHKGEDDLPYQRF
HHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHH
IDDARTKLTNRTTDCAEIQRVLGIRLGGAWCASCRQQTDCNDAMEIMD
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCC
>Mature Secondary Structure
MQTTTPYTRTRVGEYLREQGLINQQKLDVALQEQAITGEKLGEILIRFGFFERSHLVEAL
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHH
AHLNPESLIGETSVQVELPPELLLKYKCMILGDTGNTLYLATLHPHPREVLEAIGKLLDL
HHCCHHHHCCCCEEEEECCHHHHEEEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHH
SMTLTLVPIRHTIITEHLATLERSMHATRRGQDDTDINKQLTAIIHDALEDGASDIHLET
HHEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
SEKSLHVRCRIDGILHMVKALPDSLSNKIFSRIKDMSGMNVSDKMVPQDGSFSMNYRGRN
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCC
VDFRVSTIPSTYGEKATIRILDKEKMMIGVQEIGISAINDWLELARLTTGLILVCGATGS
EEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKTTTLYSTVRHLNLLEKAVYFVEDPVEYRVPFVTQVQVNRRINLDFAAFGRTVLRHDPD
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCEECCCHHHHCCHHHHCCCC
VVVVGEVRDSETAENALWLADTGHLVYATLHTNDIPSTILRLKDLGAEIPKLSYALRGIL
EEEEECCCCCCCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHH
VQKLARKLCAQCEGAGCEECKQTGYKGRTLLTEFARLNSPKDIYRLIDHKGEDDLPYQRF
HHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHH
IDDARTKLTNRTTDCAEIQRVLGIRLGGAWCASCRQQTDCNDAMEIMD
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]