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The map label for this gene is epsF [H]

Identifier: 189424037

GI number: 189424037

Start: 1010144

End: 1011307

Strand: Reverse

Name: epsF [H]

Synonym: Glov_0971

Alternate gene names: 189424037

Gene position: 1011307-1010144 (Counterclockwise)

Preceding gene: 189424038

Following gene: 189424036

Centisome position: 25.81

GC content: 53.61

Gene sequence:

>1164_bases
ATGCATCAGGTCACCTACTTTGACAAAGGGGTCCGCAAAGAGGCGGAATTCATCGGTTCAGCCGCCGAGATCAGAGAACG
GCTGATCAGCGAAGGCAAGGTAGTGCTTTCCATCAAAAAGCCGCTCACCCTGTTTCAGCCCAAGGCCAAACGCAAAGAAC
TGGCAGCGGTGCTGGTGGCGATCGGGGATCTGCTGCAGGCAGGCGTACCGCTCTCTAAGACGCTGGAAACCACCATCCAG
TCACTACCCCGTGCTTCCTCACTGATCACAATCTTAAACGCCATCAAGACCGCCGTACAACAGGGCCGCGAGCTCTCATC
CGCCATGACCGCCTACCACTCGGTCTTTGGCAGCACCACCATCTCCATGATCCAGGCCGGAGAGAAGTCGGGACGGCTGG
CAGAAAGCCTGCTGGGGGCGGCGCAGTACATCACCAACATTGAAGCGAGCCAGAGCGAGATGAAAAAAACGCTCTCCTAC
CCGCTGGTGATCTTTATCGTATCGGTGATTGCCCTGGTGGTAAACACTCAGGTGGTGATCCCCAAGATACTGGCCTCGCA
ACTGTTCAAGACCGCCCTGAAGGGGCAGACCAACTTTTTCATCCAGGCCTTGTCGGCCCTTTCGCATATTGTGCCGGCAG
TTTTTGCCGCCTTGGCGATTGTGACGGTGGTTGTGGCAGTGCTCTACAAAAGCCGCCAGCAAGAGATGGAAAAATTACTG
CTCTCTGTCCCGCTGATTCGGGAGCTGTTTTTCTATCAAGGTTTTTATGTGGCCTTCTTTTCCATGGCAAAACTGATGGA
GACCGGAATTCAGTTATCCCCGGCCCTAGAAATCGTGCAGGACACCTCGCGAACCATCGCCATTCGCACAGAATTTGACA
ATGCCATTAAAAAGGTAAAGGAGGGAGAAAGCTTCTCCTACGGCTTTACCTGCTTAAACCCAATTGAGAAAACCATGCTG
GATGTTGCCCAGAACTCAAACCGAGTCACAGAAAACCTGTCCTTGGTGGCCGACCGTTTTTACCGTGGCTACATGGAAAA
GGTCAAGGCGCTGGGGCCCAGGGTCTATGCCTTTGCCCTGATCTTTGCCGGTGGCATCTTTCTTCTGATGGTGATGGGCA
TCATGATCCCGTACAGCAAACTACTTGGAGGACTGCATGGCTAA

Upstream 100 bases:

>100_bases
AGATCCAGCGGGTATTGGGAATTCGCCTGGGTGGCGCCTGGTGTGCTTCCTGCCGTCAGCAGACCGACTGTAACGATGCC
ATGGAGATCATGGATTAGCC

Downstream 100 bases:

>100_bases
CGATGCAGCGCTGGACTCACAGCCTGATCTTGCACCAGACGAGCGGCCGGTAATCAGGAAAGTAAAAACAAAGCCCGTGT
CAGATCCACCACCGGTTTTA

Product: type II secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MHQVTYFDKGVRKEAEFIGSAAEIRERLISEGKVVLSIKKPLTLFQPKAKRKELAAVLVAIGDLLQAGVPLSKTLETTIQ
SLPRASSLITILNAIKTAVQQGRELSSAMTAYHSVFGSTTISMIQAGEKSGRLAESLLGAAQYITNIEASQSEMKKTLSY
PLVIFIVSVIALVVNTQVVIPKILASQLFKTALKGQTNFFIQALSALSHIVPAVFAALAIVTVVVAVLYKSRQQEMEKLL
LSVPLIRELFFYQGFYVAFFSMAKLMETGIQLSPALEIVQDTSRTIAIRTEFDNAIKKVKEGESFSYGFTCLNPIEKTML
DVAQNSNRVTENLSLVADRFYRGYMEKVKALGPRVYAFALIFAGGIFLLMVMGIMIPYSKLLGGLHG

Sequences:

>Translated_387_residues
MHQVTYFDKGVRKEAEFIGSAAEIRERLISEGKVVLSIKKPLTLFQPKAKRKELAAVLVAIGDLLQAGVPLSKTLETTIQ
SLPRASSLITILNAIKTAVQQGRELSSAMTAYHSVFGSTTISMIQAGEKSGRLAESLLGAAQYITNIEASQSEMKKTLSY
PLVIFIVSVIALVVNTQVVIPKILASQLFKTALKGQTNFFIQALSALSHIVPAVFAALAIVTVVVAVLYKSRQQEMEKLL
LSVPLIRELFFYQGFYVAFFSMAKLMETGIQLSPALEIVQDTSRTIAIRTEFDNAIKKVKEGESFSYGFTCLNPIEKTML
DVAQNSNRVTENLSLVADRFYRGYMEKVKALGPRVYAFALIFAGGIFLLMVMGIMIPYSKLLGGLHG
>Mature_387_residues
MHQVTYFDKGVRKEAEFIGSAAEIRERLISEGKVVLSIKKPLTLFQPKAKRKELAAVLVAIGDLLQAGVPLSKTLETTIQ
SLPRASSLITILNAIKTAVQQGRELSSAMTAYHSVFGSTTISMIQAGEKSGRLAESLLGAAQYITNIEASQSEMKKTLSY
PLVIFIVSVIALVVNTQVVIPKILASQLFKTALKGQTNFFIQALSALSHIVPAVFAALAIVTVVVAVLYKSRQQEMEKLL
LSVPLIRELFFYQGFYVAFFSMAKLMETGIQLSPALEIVQDTSRTIAIRTEFDNAIKKVKEGESFSYGFTCLNPIEKTML
DVAQNSNRVTENLSLVADRFYRGYMEKVKALGPRVYAFALIFAGGIFLLMVMGIMIPYSKLLGGLHG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=290, Percent_Identity=25.1724137931034, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 42463; Mature: 42463

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQVTYFDKGVRKEAEFIGSAAEIRERLISEGKVVLSIKKPLTLFQPKAKRKELAAVLVA
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCHHHCCCHHHHHHHHHHHH
IGDLLQAGVPLSKTLETTIQSLPRASSLITILNAIKTAVQQGRELSSAMTAYHSVFGSTT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISMIQAGEKSGRLAESLLGAAQYITNIEASQSEMKKTLSYPLVIFIVSVIALVVNTQVVI
HHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
PKILASQLFKTALKGQTNFFIQALSALSHIVPAVFAALAIVTVVVAVLYKSRQQEMEKLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVPLIRELFFYQGFYVAFFSMAKLMETGIQLSPALEIVQDTSRTIAIRTEFDNAIKKVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
EGESFSYGFTCLNPIEKTMLDVAQNSNRVTENLSLVADRFYRGYMEKVKALGPRVYAFAL
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
IFAGGIFLLMVMGIMIPYSKLLGGLHG
HHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHQVTYFDKGVRKEAEFIGSAAEIRERLISEGKVVLSIKKPLTLFQPKAKRKELAAVLVA
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCHHHCCCHHHHHHHHHHHH
IGDLLQAGVPLSKTLETTIQSLPRASSLITILNAIKTAVQQGRELSSAMTAYHSVFGSTT
HHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISMIQAGEKSGRLAESLLGAAQYITNIEASQSEMKKTLSYPLVIFIVSVIALVVNTQVVI
HHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
PKILASQLFKTALKGQTNFFIQALSALSHIVPAVFAALAIVTVVVAVLYKSRQQEMEKLL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSVPLIRELFFYQGFYVAFFSMAKLMETGIQLSPALEIVQDTSRTIAIRTEFDNAIKKVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHH
EGESFSYGFTCLNPIEKTMLDVAQNSNRVTENLSLVADRFYRGYMEKVKALGPRVYAFAL
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
IFAGGIFLLMVMGIMIPYSKLLGGLHG
HHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]