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The map label for this gene is ispD [H]

Identifier: 189423939

GI number: 189423939

Start: 897261

End: 897977

Strand: Reverse

Name: ispD [H]

Synonym: Glov_0872

Alternate gene names: 189423939

Gene position: 897977-897261 (Counterclockwise)

Preceding gene: 189423940

Following gene: 189423938

Centisome position: 22.92

GC content: 56.76

Gene sequence:

>717_bases
ATGAACAAAACGCGTGCCTTTGCCTTGATTCCCGCCGCCGGAATGGGAAAACGGATGGGAGCCTCCATGAACAAGCAGTA
CCTGCAACTGGGAGGCATACCGATTGTTGCCCGCACCCTGCAGGTATTCCAGGACAGCCCGCTCATCAGCGGCATCATAC
TGGTTATCCCAGAAGATGAGATCCCTTACTGCCGGCGTGAAGTTGTCGAAAAATACCGGTTTTCAAAGGTGCTGGATGTT
GTTTCCGGCGGTTCCGAGCGCCAGCATTCGGTACTGAACGGACTGCAGGCACTGCAGGACCATACCGCGCCTGACGATAT
CATCCTGATCCATGACGGTGTCCGCCCGTTTATTGACGAACTGATCCTGCAGCAGTCCATTGACCTGGCCACCACCGGGG
TCGGTGCCCTGGTTGCCGTTCAGACCAAAGACACCATCAAGGTGGTACACGATGGTACCGTCGTCTCAACACCGGATCGT
TCTACCCTCTGGCAGGCCCAGACCCCCCAAAGCTTCAAGTTTGGGCAGATACTGGCAGCCCACCACCAGGCGCTGCAGGA
AGGCTTCCTGGGAACCGACGACTGTTCCCTGCTGGAGCGCTTCAACGGGACCGTTAGAATCGTGAACGGCAGCTATCGAA
ACATAAAGATCACCACTCCGGAAGACCTGGTGCTTGCCGAGGCGTTTCTAAACCAGACAGCTGGAGGCCGGCCATAA

Upstream 100 bases:

>100_bases
GGCTTCAGAAGGACCGTTTTCTGCTGGATGTGCGCACCCTGAGCGACAACGACATCCCCTTCCTGGCCTCAGCCTTCTGC
CAGGCCCTTGAGCTGACCTT

Downstream 100 bases:

>100_bases
CATGGGCATCAAACAACTTCTGATTGTCGCAAACGACACCAGCGCCGCCCAACTGGCATTCCAGCTGCTCGAAGCAGGGC
CGTACCAGCTGCACCTGGCC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNKTRAFALIPAAGMGKRMGASMNKQYLQLGGIPIVARTLQVFQDSPLISGIILVIPEDEIPYCRREVVEKYRFSKVLDV
VSGGSERQHSVLNGLQALQDHTAPDDIILIHDGVRPFIDELILQQSIDLATTGVGALVAVQTKDTIKVVHDGTVVSTPDR
STLWQAQTPQSFKFGQILAAHHQALQEGFLGTDDCSLLERFNGTVRIVNGSYRNIKITTPEDLVLAEAFLNQTAGGRP

Sequences:

>Translated_238_residues
MNKTRAFALIPAAGMGKRMGASMNKQYLQLGGIPIVARTLQVFQDSPLISGIILVIPEDEIPYCRREVVEKYRFSKVLDV
VSGGSERQHSVLNGLQALQDHTAPDDIILIHDGVRPFIDELILQQSIDLATTGVGALVAVQTKDTIKVVHDGTVVSTPDR
STLWQAQTPQSFKFGQILAAHHQALQEGFLGTDDCSLLERFNGTVRIVNGSYRNIKITTPEDLVLAEAFLNQTAGGRP
>Mature_238_residues
MNKTRAFALIPAAGMGKRMGASMNKQYLQLGGIPIVARTLQVFQDSPLISGIILVIPEDEIPYCRREVVEKYRFSKVLDV
VSGGSERQHSVLNGLQALQDHTAPDDIILIHDGVRPFIDELILQQSIDLATTGVGALVAVQTKDTIKVVHDGTVVSTPDR
STLWQAQTPQSFKFGQILAAHHQALQEGFLGTDDCSLLERFNGTVRIVNGSYRNIKITTPEDLVLAEAFLNQTAGGRP

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=26.1802575107296, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI157671913, Length=128, Percent_Identity=28.90625, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=33.6244541484716, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26001; Mature: 26001

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTRAFALIPAAGMGKRMGASMNKQYLQLGGIPIVARTLQVFQDSPLISGIILVIPEDE
CCCCEEEEEECCCCCCHHHCCHHCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
IPYCRREVVEKYRFSKVLDVVSGGSERQHSVLNGLQALQDHTAPDDIILIHDGVRPFIDE
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
LILQQSIDLATTGVGALVAVQTKDTIKVVHDGTVVSTPDRSTLWQAQTPQSFKFGQILAA
HHHHHCCCHHHCCCCEEEEEECCCEEEEEECCEEEECCCCCCEECCCCCCCCHHHHHHHH
HHQALQEGFLGTDDCSLLERFNGTVRIVNGSYRNIKITTPEDLVLAEAFLNQTAGGRP
HHHHHHHCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKTRAFALIPAAGMGKRMGASMNKQYLQLGGIPIVARTLQVFQDSPLISGIILVIPEDE
CCCCEEEEEECCCCCCHHHCCHHCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
IPYCRREVVEKYRFSKVLDVVSGGSERQHSVLNGLQALQDHTAPDDIILIHDGVRPFIDE
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
LILQQSIDLATTGVGALVAVQTKDTIKVVHDGTVVSTPDRSTLWQAQTPQSFKFGQILAA
HHHHHCCCHHHCCCCEEEEEECCCEEEEEECCEEEECCCCCCEECCCCCCCCHHHHHHHH
HHQALQEGFLGTDDCSLLERFNGTVRIVNGSYRNIKITTPEDLVLAEAFLNQTAGGRP
HHHHHHHCCCCCCHHHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA