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The map label for this gene is hemB [H]

Identifier: 189423757

GI number: 189423757

Start: 696918

End: 697898

Strand: Reverse

Name: hemB [H]

Synonym: Glov_0688

Alternate gene names: 189423757

Gene position: 697898-696918 (Counterclockwise)

Preceding gene: 189423759

Following gene: 189423756

Centisome position: 17.81

GC content: 56.98

Gene sequence:

>981_bases
ATGTTTTCATCCCGTTTCAGGGCTCGCCGGATCCGTGGCAAAGAGGTATTCCGCCGGATGGTACGAGAGACTACACTCTC
AGCCAACGATCTGATTTATCCGATGTTTTCAGCCTTTGGCAGTGGTATCCGCAAAGAGGTTTCCTCCATGCCAGGTATCT
TTCAGCAATCCATTGAGCATATCGTTGCCGAGGCCAAAGAAGTCCATGGGCTGGGCATACCGGCAGTGATCCTGTTCGGC
ATTCCCGAGACCAAGGATGCGGTTGGAAGCGACGCCTATGCCGAGCATGGCATTATCCAGGAGACAATCCGGGCAATCAA
GCGAGAGGTGCCGGGATTGGCGGTCATTACCGATGTCTGTCTGTGTGAATACACCGACCATGGCCACTGCGGCATCATCA
AAAATGGTGATGTCGACAACGATGCCACGGTCAAACTGCTGGCCCGGGAAGCCCTTTCCCACGCCCAGGCCGGTGCCGAC
ATGGTAGCCCCCTCCGATATGATGGACGGGCGGGTGGCTGCCATTCGCCAGACTCTGGACAACAACGGCTTCAGCCATAT
CCCGGTCATGAGCTATGCAGTCAAGTACGCCTCCGGCTACTACGGGCCGTTCCGCGAGGCAGCCGAATCAACGCCTCAAT
TCGGTGACCGGCGTTCCTACCAGATGGACCCTGCCAATCGTCTCGAAGCCCTGCGGGAAGCCCAGGCTGATATTGAAGAA
GGTGCCGATATCATCATGGTCAAACCGGGACTTCCCTATCTGGACATCCTGCGTGACCTGCGCAATGAATATGCCATGCC
GCTGGCTGTCTACAATGTCTCCGGCGAATACAGCATGATCAAGGGTGCGGCGGCAAACGGCTGGATCGATGAAGAGCGGG
TGGTGATGGAGACCATGCTGGGCTTCAAGCGGGCCGGGGCAGATCTGATCATCACCTATCACGCCAAGGATGTGGCACGC
TGGCTGAAAGGCGGCATCTAG

Upstream 100 bases:

>100_bases
TTTTTTTCTTGTCAACCACTCTCCTGACCTTGCATTCGTCAAATCAGACGTTGTATGATGCCATTGGCATAAAAAAAGCA
AAAACCGGAGGTTAGCGCCA

Downstream 100 bases:

>100_bases
GCTCTGGTTCGCTTGCAACAAACGCAGTACAGGGTGCAGGGATGCTGACTGACATCATCGTACATATGGACAGGGGAGCG
GGATGTACAGCCAGATTGAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MFSSRFRARRIRGKEVFRRMVRETTLSANDLIYPMFSAFGSGIRKEVSSMPGIFQQSIEHIVAEAKEVHGLGIPAVILFG
IPETKDAVGSDAYAEHGIIQETIRAIKREVPGLAVITDVCLCEYTDHGHCGIIKNGDVDNDATVKLLAREALSHAQAGAD
MVAPSDMMDGRVAAIRQTLDNNGFSHIPVMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPANRLEALREAQADIEE
GADIIMVKPGLPYLDILRDLRNEYAMPLAVYNVSGEYSMIKGAAANGWIDEERVVMETMLGFKRAGADLIITYHAKDVAR
WLKGGI

Sequences:

>Translated_326_residues
MFSSRFRARRIRGKEVFRRMVRETTLSANDLIYPMFSAFGSGIRKEVSSMPGIFQQSIEHIVAEAKEVHGLGIPAVILFG
IPETKDAVGSDAYAEHGIIQETIRAIKREVPGLAVITDVCLCEYTDHGHCGIIKNGDVDNDATVKLLAREALSHAQAGAD
MVAPSDMMDGRVAAIRQTLDNNGFSHIPVMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPANRLEALREAQADIEE
GADIIMVKPGLPYLDILRDLRNEYAMPLAVYNVSGEYSMIKGAAANGWIDEERVVMETMLGFKRAGADLIITYHAKDVAR
WLKGGI
>Mature_326_residues
MFSSRFRARRIRGKEVFRRMVRETTLSANDLIYPMFSAFGSGIRKEVSSMPGIFQQSIEHIVAEAKEVHGLGIPAVILFG
IPETKDAVGSDAYAEHGIIQETIRAIKREVPGLAVITDVCLCEYTDHGHCGIIKNGDVDNDATVKLLAREALSHAQAGAD
MVAPSDMMDGRVAAIRQTLDNNGFSHIPVMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPANRLEALREAQADIEE
GADIIMVKPGLPYLDILRDLRNEYAMPLAVYNVSGEYSMIKGAAANGWIDEERVVMETMLGFKRAGADLIITYHAKDVAR
WLKGGI

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=305, Percent_Identity=44.9180327868852, Blast_Score=271, Evalue=4e-73,
Organism=Escherichia coli, GI87081728, Length=313, Percent_Identity=49.8402555910543, Blast_Score=301, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=313, Percent_Identity=42.8115015974441, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI21358291, Length=301, Percent_Identity=40.8637873754153, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35968; Mature: 35968

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSRFRARRIRGKEVFRRMVRETTLSANDLIYPMFSAFGSGIRKEVSSMPGIFQQSIEH
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IVAEAKEVHGLGIPAVILFGIPETKDAVGSDAYAEHGIIQETIRAIKREVPGLAVITDVC
HHHHHHHHCCCCCCEEEEECCCCCHHHCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHH
LCEYTDHGHCGIIKNGDVDNDATVKLLAREALSHAQAGADMVAPSDMMDGRVAAIRQTLD
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHH
NNGFSHIPVMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPANRLEALREAQADIEE
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GADIIMVKPGLPYLDILRDLRNEYAMPLAVYNVSGEYSMIKGAAANGWIDEERVVMETML
CCCEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHH
GFKRAGADLIITYHAKDVARWLKGGI
HHHHCCCCEEEEECHHHHHHHHHCCC
>Mature Secondary Structure
MFSSRFRARRIRGKEVFRRMVRETTLSANDLIYPMFSAFGSGIRKEVSSMPGIFQQSIEH
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IVAEAKEVHGLGIPAVILFGIPETKDAVGSDAYAEHGIIQETIRAIKREVPGLAVITDVC
HHHHHHHHCCCCCCEEEEECCCCCHHHCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHH
LCEYTDHGHCGIIKNGDVDNDATVKLLAREALSHAQAGADMVAPSDMMDGRVAAIRQTLD
HHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHHHHHHH
NNGFSHIPVMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPANRLEALREAQADIEE
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GADIIMVKPGLPYLDILRDLRNEYAMPLAVYNVSGEYSMIKGAAANGWIDEERVVMETML
CCCEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHH
GFKRAGADLIITYHAKDVARWLKGGI
HHHHCCCCEEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]