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The map label for this gene is nifK [H]

Identifier: 189423717

GI number: 189423717

Start: 653185

End: 654654

Strand: Reverse

Name: nifK [H]

Synonym: Glov_0648

Alternate gene names: 189423717

Gene position: 654654-653185 (Counterclockwise)

Preceding gene: 189423718

Following gene: 189423716

Centisome position: 16.71

GC content: 53.61

Gene sequence:

>1470_bases
ATGTCAAACTTACTAGGACTCGAAGTAAAGAAGATAGTCGAGACCACACCCGAAGAAGTAGACCGGGTTCAGAACTGGAT
AAACACTGAAGAATACCGGGAGAAAAACTTTGCCCGCCAGGCACTGGTAATCAACCCGGCCCATGCCTGCCAACCTTTGG
GAGCCCAGCTGGCTGCCCACGGTTTTGAAGGCACCCTGCCGTTTGTGCATGGTTCACAAGGTTGTGCTTCCTACTACCGC
TCAACCCTTAACCGTCACTTCCGCGAACCTGCTCCGGCAGTATCAGACGCGATGACCGAGGACGGTGCGGTGTTTGGCGG
CCAGAATAACCTGCACGAAGGTCTGGAAAACGCCGTTGCCCTGTACAAGCCAAAAATGATAGCCGTCTTCACCTCCTGCA
TGCCTGAGGTTATCGGCGACGACTTGACCGCCTTCATCAAGAATGCCCGTCAGAAGGGTCATGTGCCCAAAGATATGCCG
GTGCCTTATGCCAACACCCCCAGCTTCAACGGCACCCATATCCACGGTTATGATTCAATGCTGGTGTCGATTCTCCAGAA
CCTGACCGAAGGAAAGCAGATCGAAGGACGCTGCACCGGCAAGCTGAATCTGATCGCTGGCTGCGACTTCAATACCGGCG
ACTACCGTGAGTACAAGCGAATTCTGACAGAGTTCGGCATCCCCCACACCGTTCTGGCAGATATCAGCGACAGCTTTGAT
TCACCGTGTGATGGTCACTACCATATTTACGCCGGTGGCACCAAGCTGGAGGATGCTGCAGACTCCATCAACGGCAAGGC
AACGATAACCCTGGGGCCCTACGCAACACCCAAGACCTTCAGCTTTATCAAGGACAACTACTCCGGTAAGCATGTCAGCA
TGCCGATGCCGATGGGTATTGAAAAGACCGATGCCTTTATCATGAAAGTGGCTGAGCTGTTCGGCAAGGAAGTGCCTGCT
TCACTGAAAAACGAGCGTGGGCGTGCCGTAGATGCCATCACTGACGCACATCAGTATATTCATGGCAAGAAGTTTGCCGT
CTACGGTGATCCGGACTACCTGCTGGGCTATGTCTCCTTCCTGCTGGAAATGGGCGCCCATCCACACCACATCCTCTGCA
GCCGTGGTTCCAAGAAGCTTGAAAAAGAGCTGCAGGCACTTCTTGACGGTTCCGAGTTTGGTAAAGGCTGCAAGATTTAC
ATGAACAAGGATCTCTGGCATCTGCGTTCACTGCTGATGACCGATCCGGTCGATGCCATCATCGGCGACAGCCACGGCAA
GTTTGCCGCCCGTGATGCCAAGATCCCGCTGTTCCGCTTCGGCTTCCCGATTTTTGATCGGGTCAACAAGCATCGCTACC
CGATCATCGGTTACCAGGGTGCCGTCAACATGCTGACCGAGATCTGCAACAAGTTCATCGACATCAAGGATGAGACCTGT
GAAGATCGTGACTTTGAGTTGATGCGCTAG

Upstream 100 bases:

>100_bases
GAAGCTGGTGCAGTCACCATTCTAACGCTGTCAGAACACGCACGATACTTACCTACAATGGCGGTGTTACGGATGAGTTT
CCCGCCAAAGGAGATATACC

Downstream 100 bases:

>100_bases
CACACATCAGGTTGTAACCTTAGCATTTAACCAGCCAGCGGCTGTCCGATATCGGCCAGCCGCTGGATTTGCTTGTTATT
TATACACTCCAAAAACAAAA

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSNLLGLEVKKIVETTPEEVDRVQNWINTEEYREKNFARQALVINPAHACQPLGAQLAAHGFEGTLPFVHGSQGCASYYR
STLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAVALYKPKMIAVFTSCMPEVIGDDLTAFIKNARQKGHVPKDMP
VPYANTPSFNGTHIHGYDSMLVSILQNLTEGKQIEGRCTGKLNLIAGCDFNTGDYREYKRILTEFGIPHTVLADISDSFD
SPCDGHYHIYAGGTKLEDAADSINGKATITLGPYATPKTFSFIKDNYSGKHVSMPMPMGIEKTDAFIMKVAELFGKEVPA
SLKNERGRAVDAITDAHQYIHGKKFAVYGDPDYLLGYVSFLLEMGAHPHHILCSRGSKKLEKELQALLDGSEFGKGCKIY
MNKDLWHLRSLLMTDPVDAIIGDSHGKFAARDAKIPLFRFGFPIFDRVNKHRYPIIGYQGAVNMLTEICNKFIDIKDETC
EDRDFELMR

Sequences:

>Translated_489_residues
MSNLLGLEVKKIVETTPEEVDRVQNWINTEEYREKNFARQALVINPAHACQPLGAQLAAHGFEGTLPFVHGSQGCASYYR
STLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAVALYKPKMIAVFTSCMPEVIGDDLTAFIKNARQKGHVPKDMP
VPYANTPSFNGTHIHGYDSMLVSILQNLTEGKQIEGRCTGKLNLIAGCDFNTGDYREYKRILTEFGIPHTVLADISDSFD
SPCDGHYHIYAGGTKLEDAADSINGKATITLGPYATPKTFSFIKDNYSGKHVSMPMPMGIEKTDAFIMKVAELFGKEVPA
SLKNERGRAVDAITDAHQYIHGKKFAVYGDPDYLLGYVSFLLEMGAHPHHILCSRGSKKLEKELQALLDGSEFGKGCKIY
MNKDLWHLRSLLMTDPVDAIIGDSHGKFAARDAKIPLFRFGFPIFDRVNKHRYPIIGYQGAVNMLTEICNKFIDIKDETC
EDRDFELMR
>Mature_488_residues
SNLLGLEVKKIVETTPEEVDRVQNWINTEEYREKNFARQALVINPAHACQPLGAQLAAHGFEGTLPFVHGSQGCASYYRS
TLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAVALYKPKMIAVFTSCMPEVIGDDLTAFIKNARQKGHVPKDMPV
PYANTPSFNGTHIHGYDSMLVSILQNLTEGKQIEGRCTGKLNLIAGCDFNTGDYREYKRILTEFGIPHTVLADISDSFDS
PCDGHYHIYAGGTKLEDAADSINGKATITLGPYATPKTFSFIKDNYSGKHVSMPMPMGIEKTDAFIMKVAELFGKEVPAS
LKNERGRAVDAITDAHQYIHGKKFAVYGDPDYLLGYVSFLLEMGAHPHHILCSRGSKKLEKELQALLDGSEFGKGCKIYM
NKDLWHLRSLLMTDPVDAIIGDSHGKFAARDAKIPLFRFGFPIFDRVNKHRYPIIGYQGAVNMLTEICNKFIDIKDETCE
DRDFELMR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54411; Mature: 54279

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLLGLEVKKIVETTPEEVDRVQNWINTEEYREKNFARQALVINPAHACQPLGAQLAAH
CCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHEEEECCHHHCCHHHHHHHHC
GFEGTLPFVHGSQGCASYYRSTLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAVA
CCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHH
LYKPKMIAVFTSCMPEVIGDDLTAFIKNARQKGHVPKDMPVPYANTPSFNGTHIHGYDSM
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCHHHH
LVSILQNLTEGKQIEGRCTGKLNLIAGCDFNTGDYREYKRILTEFGIPHTVLADISDSFD
HHHHHHHHHCCCCCCCEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
SPCDGHYHIYAGGTKLEDAADSINGKATITLGPYATPKTFSFIKDNYSGKHVSMPMPMGI
CCCCCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCCCEECCCCCCCC
EKTDAFIMKVAELFGKEVPASLKNERGRAVDAITDAHQYIHGKKFAVYGDPDYLLGYVSF
CHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
LLEMGAHPHHILCSRGSKKLEKELQALLDGSEFGKGCKIYMNKDLWHLRSLLMTDPVDAI
HHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHCCCCEEEEECCCHHHHHHHHHCCCHHHH
IGDSHGKFAARDAKIPLFRFGFPIFDRVNKHRYPIIGYQGAVNMLTEICNKFIDIKDETC
HCCCCCCEEECCCCCCHHHHCCHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCC
EDRDFELMR
CCCCCCCCC
>Mature Secondary Structure 
SNLLGLEVKKIVETTPEEVDRVQNWINTEEYREKNFARQALVINPAHACQPLGAQLAAH
CCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHEEEECCHHHCCHHHHHHHHC
GFEGTLPFVHGSQGCASYYRSTLNRHFREPAPAVSDAMTEDGAVFGGQNNLHEGLENAVA
CCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEECCCCHHHHHHHHHHH
LYKPKMIAVFTSCMPEVIGDDLTAFIKNARQKGHVPKDMPVPYANTPSFNGTHIHGYDSM
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCHHHH
LVSILQNLTEGKQIEGRCTGKLNLIAGCDFNTGDYREYKRILTEFGIPHTVLADISDSFD
HHHHHHHHHCCCCCCCEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCC
SPCDGHYHIYAGGTKLEDAADSINGKATITLGPYATPKTFSFIKDNYSGKHVSMPMPMGI
CCCCCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCCCCCEECCCCCCCC
EKTDAFIMKVAELFGKEVPASLKNERGRAVDAITDAHQYIHGKKFAVYGDPDYLLGYVSF
CHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
LLEMGAHPHHILCSRGSKKLEKELQALLDGSEFGKGCKIYMNKDLWHLRSLLMTDPVDAI
HHHHCCCCCEEEECCCHHHHHHHHHHHHCCHHCCCCEEEEECCCHHHHHHHHHCCCHHHH
IGDSHGKFAARDAKIPLFRFGFPIFDRVNKHRYPIIGYQGAVNMLTEICNKFIDIKDETC
HCCCCCCEEECCCCCCHHHHCCHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCC
EDRDFELMR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]