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The map label for this gene is gltX

Identifier: 189423654

GI number: 189423654

Start: 572101

End: 573501

Strand: Reverse

Name: gltX

Synonym: Glov_0584

Alternate gene names: 189423654

Gene position: 573501-572101 (Counterclockwise)

Preceding gene: 189423668

Following gene: 189423653

Centisome position: 14.64

GC content: 60.67

Gene sequence:

>1401_bases
ATGTCTGATCTGCGCGTTCGCTTTGCCCCTTCGCCCACCGGCTACCTGCATGTTGGCGGTGCCCGCACCGCCCTGTTCAA
CTGGCTATACGCCCGTCATTTCGGCGGCACCTTTATCCTGCGGATCGAAGATACCGACACTGAGCGTTCCACCCAGCAAT
CGGTGGATGCCATTCTGCAGGGGATGGAGTGGCTGGGGCTGGACTGGGATGAAGGGCCGTTCTACCAGACCGATAACTTT
CCGCTCTACAAGCAGCATGTCCAGAAGCTGCTGGATGAAGGCAAGGCCTACCGTTGCTGGTGCAGACCGGAGGAGCTGGA
GGCCAAGCGTGAGGCGGCCATGGCCGAGGGGCGCAAGCCCAAGTACGACGGCACCTGCCGCCACCGTCAGGACCAGCCGC
TGGATCAACCCCACGTCATCCGCTTCAAGGCCCCGGAAGAGGGAGAAACAGCCTTTGACGACCTGATCAAGGGCAGGATC
GCCTTCCCCAATGCCGAGCTGGATGATCTGATCATCAGCCGCACCGACGGCACCCCCACCTACAACTTCTGTGTGGTGAT
CGATGATGCCCTGATGCGGATCTCCCACGTCATCCGGGGCGACGACCATGTCAACAACACCCCGCGCCAGATCCAGTTGT
ATGAGGCATTGGGCTATCCGGTGCCGATCTTCGCCCATGTGCCGATGATTCTGGGCAGCGACAAGGCCCGGCTTTCCAAA
CGACATGGTGCCACCAGCGTGATCGCCTACCGTGATATGGGCTACCTGCCGGAGGCGCTGAACAACTATCTGGTACGGCT
GGGCTGGAGTAACGGCGATGATGAAATTTTCAGCCGTGAGGAGATGGTGCAGAAGTTTGATATCGCCAATGTGGGCCGTT
CCCCCTCGGTCTTCAACCCGGACAAACTGAACTGGCTGAACGCCCATTACATCAAAACCGGCAACCCCGCCCGGCTGGCT
GAACTACTGCAACCCCATCTGGCAGGCCGTGGTGTGGCGGACTGTTCAACCCCTGATCTGGCCGGCGTCATCAGCACCCT
GCAGGAGCGGGCCCAGACCCTGGAAGAGATGGCCGAGCGGGCCTTGTTCTACTACCAGGCACCGCAACAGTATGACGAGG
CGGCCCTGAGCAAGTTTGACAAACCGCATCTGGCAGCAGTCTTTAGCGCTGTTGCGGCCAGGCTCTCTGCAACAACCGCT
GCTGCCGCACCGGAGTTCGATACGCTGCTGAAGGAGATTTGCGCCGAAGGCGGATGGAAGATGCCCCATGTCGGTCAGCC
TCTGCGGATCGCCCTGTCCGGCAGCACCCAGGCACCCGGCATCGGCGAGATCATCACGGCCCTGGGAGTTAACGAGACGA
TTGCACGTATTGAACGGGCCAGAGAGTTCCTGGCACACTGA

Upstream 100 bases:

>100_bases
CCCCGGCCCAGACCTTAACAATGCTTGACAAGTAGATGCCTGATAAGGCTTATTTCATTACACGGCATGACTTAGGAATT
ATTCTACAGGAGCTTTACCG

Downstream 100 bases:

>100_bases
CGGTCTGCGGTTTTCGGGGCGACAGGGTGGGAGACCCATGGATATACTTGCCATAACTGACATACCGCGTATGACTGCAC
TGTTTGAACGGCTTGCCCGG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDLRVRFAPSPTGYLHVGGARTALFNWLYARHFGGTFILRIEDTDTERSTQQSVDAILQGMEWLGLDWDEGPFYQTDNF
PLYKQHVQKLLDEGKAYRCWCRPEELEAKREAAMAEGRKPKYDGTCRHRQDQPLDQPHVIRFKAPEEGETAFDDLIKGRI
AFPNAELDDLIISRTDGTPTYNFCVVIDDALMRISHVIRGDDHVNNTPRQIQLYEALGYPVPIFAHVPMILGSDKARLSK
RHGATSVIAYRDMGYLPEALNNYLVRLGWSNGDDEIFSREEMVQKFDIANVGRSPSVFNPDKLNWLNAHYIKTGNPARLA
ELLQPHLAGRGVADCSTPDLAGVISTLQERAQTLEEMAERALFYYQAPQQYDEAALSKFDKPHLAAVFSAVAARLSATTA
AAAPEFDTLLKEICAEGGWKMPHVGQPLRIALSGSTQAPGIGEIITALGVNETIARIERAREFLAH

Sequences:

>Translated_466_residues
MSDLRVRFAPSPTGYLHVGGARTALFNWLYARHFGGTFILRIEDTDTERSTQQSVDAILQGMEWLGLDWDEGPFYQTDNF
PLYKQHVQKLLDEGKAYRCWCRPEELEAKREAAMAEGRKPKYDGTCRHRQDQPLDQPHVIRFKAPEEGETAFDDLIKGRI
AFPNAELDDLIISRTDGTPTYNFCVVIDDALMRISHVIRGDDHVNNTPRQIQLYEALGYPVPIFAHVPMILGSDKARLSK
RHGATSVIAYRDMGYLPEALNNYLVRLGWSNGDDEIFSREEMVQKFDIANVGRSPSVFNPDKLNWLNAHYIKTGNPARLA
ELLQPHLAGRGVADCSTPDLAGVISTLQERAQTLEEMAERALFYYQAPQQYDEAALSKFDKPHLAAVFSAVAARLSATTA
AAAPEFDTLLKEICAEGGWKMPHVGQPLRIALSGSTQAPGIGEIITALGVNETIARIERAREFLAH
>Mature_465_residues
SDLRVRFAPSPTGYLHVGGARTALFNWLYARHFGGTFILRIEDTDTERSTQQSVDAILQGMEWLGLDWDEGPFYQTDNFP
LYKQHVQKLLDEGKAYRCWCRPEELEAKREAAMAEGRKPKYDGTCRHRQDQPLDQPHVIRFKAPEEGETAFDDLIKGRIA
FPNAELDDLIISRTDGTPTYNFCVVIDDALMRISHVIRGDDHVNNTPRQIQLYEALGYPVPIFAHVPMILGSDKARLSKR
HGATSVIAYRDMGYLPEALNNYLVRLGWSNGDDEIFSREEMVQKFDIANVGRSPSVFNPDKLNWLNAHYIKTGNPARLAE
LLQPHLAGRGVADCSTPDLAGVISTLQERAQTLEEMAERALFYYQAPQQYDEAALSKFDKPHLAAVFSAVAARLSATTAA
AAPEFDTLLKEICAEGGWKMPHVGQPLRIALSGSTQAPGIGEIITALGVNETIARIERAREFLAH

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.7692307692308, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI62241042, Length=255, Percent_Identity=25.4901960784314, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4826960, Length=297, Percent_Identity=24.5791245791246, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=51.8358531317495, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=34.297520661157, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1786895, Length=333, Percent_Identity=23.7237237237237, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71993394, Length=486, Percent_Identity=31.6872427983539, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=32.7235772357724, Blast_Score=272, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6321192, Length=232, Percent_Identity=28.0172413793103, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324742, Length=218, Percent_Identity=30.7339449541284, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=33.1288343558282, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24649466, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI17864488, Length=306, Percent_Identity=25.4901960784314, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_GEOLS (B3E399)

Other databases:

- EMBL:   CP001089
- RefSeq:   YP_001950831.1
- ProteinModelPortal:   B3E399
- SMR:   B3E399
- GeneID:   6367962
- GenomeReviews:   CP001089_GR
- KEGG:   glo:Glov_0584
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52156; Mature: 52024

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLRVRFAPSPTGYLHVGGARTALFNWLYARHFGGTFILRIEDTDTERSTQQSVDAILQ
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHH
GMEWLGLDWDEGPFYQTDNFPLYKQHVQKLLDEGKAYRCWCRPEELEAKREAAMAEGRKP
CHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCC
KYDGTCRHRQDQPLDQPHVIRFKAPEEGETAFDDLIKGRIAFPNAELDDLIISRTDGTPT
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEECCCCCCCHHEEECCCCCCC
YNFCVVIDDALMRISHVIRGDDHVNNTPRQIQLYEALGYPVPIFAHVPMILGSDKARLSK
EEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEHHHHHCCCCCHHHHCCHHCCCCHHHHHH
RHGATSVIAYRDMGYLPEALNNYLVRLGWSNGDDEIFSREEMVQKFDIANVGRSPSVFNP
HCCCCEEEEECCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
DKLNWLNAHYIKTGNPARLAELLQPHLAGRGVADCSTPDLAGVISTLQERAQTLEEMAER
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALFYYQAPQQYDEAALSKFDKPHLAAVFSAVAARLSATTAAAAPEFDTLLKEICAEGGWK
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
MPHVGQPLRIALSGSTQAPGIGEIITALGVNETIARIERAREFLAH
CCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDLRVRFAPSPTGYLHVGGARTALFNWLYARHFGGTFILRIEDTDTERSTQQSVDAILQ
CCCEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHH
GMEWLGLDWDEGPFYQTDNFPLYKQHVQKLLDEGKAYRCWCRPEELEAKREAAMAEGRKP
CHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCC
KYDGTCRHRQDQPLDQPHVIRFKAPEEGETAFDDLIKGRIAFPNAELDDLIISRTDGTPT
CCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEECCCCCCCHHEEECCCCCCC
YNFCVVIDDALMRISHVIRGDDHVNNTPRQIQLYEALGYPVPIFAHVPMILGSDKARLSK
EEEEEEEHHHHHHHHHHHCCCCCCCCCCHHEEHHHHHCCCCCHHHHCCHHCCCCHHHHHH
RHGATSVIAYRDMGYLPEALNNYLVRLGWSNGDDEIFSREEMVQKFDIANVGRSPSVFNP
HCCCCEEEEECCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
DKLNWLNAHYIKTGNPARLAELLQPHLAGRGVADCSTPDLAGVISTLQERAQTLEEMAER
CCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALFYYQAPQQYDEAALSKFDKPHLAAVFSAVAARLSATTAAAAPEFDTLLKEICAEGGWK
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
MPHVGQPLRIALSGSTQAPGIGEIITALGVNETIARIERAREFLAH
CCCCCCCEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA