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The map label for this gene is metK [H]

Identifier: 189423420

GI number: 189423420

Start: 320612

End: 321778

Strand: Reverse

Name: metK [H]

Synonym: Glov_0349

Alternate gene names: 189423420

Gene position: 321778-320612 (Counterclockwise)

Preceding gene: 189423421

Following gene: 189423419

Centisome position: 8.21

GC content: 58.95

Gene sequence:

>1167_bases
ATGGCTGAAAAATTCATCTTCACCTCTGAATCCGTATCCGAAGGTCACCCTGACAAAATGGCTGACCAGATCTCCGACAG
CATCCTGGACGCGATCCTGGCCCAGGACCCCAAGGCCCGCGTGGCCTGCGAGACCATGGTGACCACCGGTATGGCCGTGA
TTGCCGGTGAAATCACCACCACCGCGGTGGTGAACTATGCCGAGATCGTCCGCAACACCATCAAGGAGATCGGCTACTGC
GGTTCCGAGACCGGCTTTGACTATGAGACCTGCTCTGTGCTGGTCTCCCTTGACCGCCAGTCCCCGGACATCTCCCAGGG
TGTCACCGAGGGCGAGGGGATGTTCAAGGAGCAGGGAGCCGGTGACCAGGGCCTGATGTTCGGCTATGCCTGCAACGAAA
CCCCGGAACTGATGCCGATGCCGATCCAGCTTTCCCATCAGCTGGTCAAGCGGCTGGCCGATGTCCGCAAATCCGGCCTG
CTGAAATTCCTGCGTCCCGATGCAAAATCCCAGGTCTCGGTTGAGTATGACAACGGCAAGCCGGTACGGGTCAATACCGT
GGTCATCTCCACCCAGCACACTCCTGATGTAACCCATGAAACCATTGTGGAAGGGGTCATGGATGAGGTGATCAAAAAGG
TAATCCCTGCCCACCTGATGGATGAGCAGACCCGCTTCTTCATCAACCCCACCGGCCGTTTTGTGGTGGGTGGACCGATG
GGCGACTGCGGTCTGACCGGTCGCAAGATCATTGTGGACACCTATGGCGGCATGGGCCGTCACGGCGGCGGAGCCTTCTC
CGGCAAAGATCCCTCCAAGGTTGACCGTTCCGCTGCCTACATGGGCCGCTATGTGGCCAAGAACCTGGTGGCTGCCGGCC
TGTGCGAGCGCTGTGAAGTCCAGGTGGCCTATGCCATCGGCGTGGCAGAGCCGGTCTCGATCATGGTTAATGCCTTCGGC
ACCGGCGTGGTATCTGAGCATCGCCTGTCGGAGCTGGTACGGGAGGTCTTTGACATGCGTCCCCGCGCTATTACCGAGCA
GCTTGACCTGCTGCGCCCGATCTATCAGAAGACCGCTGCCTATGGTCACTTTGGTCGTGAACTGCCTGAGTTTACCTGGG
AAAAGACCGATAAGGCAGAGATCCTGAAACAGAAGGCCGGACTGTAA

Upstream 100 bases:

>100_bases
GCAGAAACCATGCTGATGCTGAGGATCAGTAGACTGGGTTAACGAGGATTCGTTTTGTCTGGCAACAACCTATATTTTAC
GTAAAAGGAGTTTCACGCAC

Downstream 100 bases:

>100_bases
CCGGAGCCGTTAATCCTGACGTTTATGTAAGGGGCGGTCTCCAAAGAGATCGCCCCTTTTTCGTGATGATTGACAAACAT
CTAATGTATACAGTAAGGTA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MAEKFIFTSESVSEGHPDKMADQISDSILDAILAQDPKARVACETMVTTGMAVIAGEITTTAVVNYAEIVRNTIKEIGYC
GSETGFDYETCSVLVSLDRQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACNETPELMPMPIQLSHQLVKRLADVRKSGL
LKFLRPDAKSQVSVEYDNGKPVRVNTVVISTQHTPDVTHETIVEGVMDEVIKKVIPAHLMDEQTRFFINPTGRFVVGGPM
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEVQVAYAIGVAEPVSIMVNAFG
TGVVSEHRLSELVREVFDMRPRAITEQLDLLRPIYQKTAAYGHFGRELPEFTWEKTDKAEILKQKAGL

Sequences:

>Translated_388_residues
MAEKFIFTSESVSEGHPDKMADQISDSILDAILAQDPKARVACETMVTTGMAVIAGEITTTAVVNYAEIVRNTIKEIGYC
GSETGFDYETCSVLVSLDRQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACNETPELMPMPIQLSHQLVKRLADVRKSGL
LKFLRPDAKSQVSVEYDNGKPVRVNTVVISTQHTPDVTHETIVEGVMDEVIKKVIPAHLMDEQTRFFINPTGRFVVGGPM
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEVQVAYAIGVAEPVSIMVNAFG
TGVVSEHRLSELVREVFDMRPRAITEQLDLLRPIYQKTAAYGHFGRELPEFTWEKTDKAEILKQKAGL
>Mature_387_residues
AEKFIFTSESVSEGHPDKMADQISDSILDAILAQDPKARVACETMVTTGMAVIAGEITTTAVVNYAEIVRNTIKEIGYCG
SETGFDYETCSVLVSLDRQSPDISQGVTEGEGMFKEQGAGDQGLMFGYACNETPELMPMPIQLSHQLVKRLADVRKSGLL
KFLRPDAKSQVSVEYDNGKPVRVNTVVISTQHTPDVTHETIVEGVMDEVIKKVIPAHLMDEQTRFFINPTGRFVVGGPMG
DCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEVQVAYAIGVAEPVSIMVNAFGT
GVVSEHRLSELVREVFDMRPRAITEQLDLLRPIYQKTAAYGHFGRELPEFTWEKTDKAEILKQKAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=374, Percent_Identity=59.6256684491979, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=58.2887700534759, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI1789311, Length=385, Percent_Identity=66.2337662337662, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17538498, Length=383, Percent_Identity=59.0078328981723, Blast_Score=445, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=58.961038961039, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17538494, Length=383, Percent_Identity=58.4856396866841, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17551082, Length=388, Percent_Identity=57.4742268041237, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32565905, Length=383, Percent_Identity=52.7415143603133, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=56.4179104477612, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=56.379821958457, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565903, Length=318, Percent_Identity=54.7169811320755, Blast_Score=331, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=67.4698795180723, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=82.5, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323209, Length=379, Percent_Identity=57.2559366754617, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320710, Length=384, Percent_Identity=55.7291666666667, Blast_Score=436, Evalue=1e-123,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=56.4304461942257, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=56.4304461942257, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=56.4304461942257, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=56.4304461942257, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=56.4304461942257, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=56.4304461942257, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=55.3805774278215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=55.3805774278215, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=368, Percent_Identity=54.3478260869565, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=56.5573770491803, Blast_Score=141, Evalue=9e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42340; Mature: 42209

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKFIFTSESVSEGHPDKMADQISDSILDAILAQDPKARVACETMVTTGMAVIAGEITT
CCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHCCCHHH
TAVVNYAEIVRNTIKEIGYCGSETGFDYETCSVLVSLDRQSPDISQGVTEGEGMFKEQGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHCCC
GDQGLMFGYACNETPELMPMPIQLSHQLVKRLADVRKSGLLKFLRPDAKSQVSVEYDNGK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
PVRVNTVVISTQHTPDVTHETIVEGVMDEVIKKVIPAHLMDEQTRFFINPTGRFVVGGPM
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCC
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCE
QVAYAIGVAEPVSIMVNAFGTGVVSEHRLSELVREVFDMRPRAITEQLDLLRPIYQKTAA
EEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YGHFGRELPEFTWEKTDKAEILKQKAGL
HHHHHHHCCCHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
AEKFIFTSESVSEGHPDKMADQISDSILDAILAQDPKARVACETMVTTGMAVIAGEITT
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHCCCHHH
TAVVNYAEIVRNTIKEIGYCGSETGFDYETCSVLVSLDRQSPDISQGVTEGEGMFKEQGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHCCC
GDQGLMFGYACNETPELMPMPIQLSHQLVKRLADVRKSGLLKFLRPDAKSQVSVEYDNGK
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
PVRVNTVVISTQHTPDVTHETIVEGVMDEVIKKVIPAHLMDEQTRFFINPTGRFVVGGPM
EEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCC
GDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVDRSAAYMGRYVAKNLVAAGLCERCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCE
QVAYAIGVAEPVSIMVNAFGTGVVSEHRLSELVREVFDMRPRAITEQLDLLRPIYQKTAA
EEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YGHFGRELPEFTWEKTDKAEILKQKAGL
HHHHHHHCCCHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA