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The map label for this gene is leuB [H]

Identifier: 189423419

GI number: 189423419

Start: 319392

End: 320483

Strand: Reverse

Name: leuB [H]

Synonym: Glov_0348

Alternate gene names: 189423419

Gene position: 320483-319392 (Counterclockwise)

Preceding gene: 189423420

Following gene: 189423418

Centisome position: 8.18

GC content: 57.23

Gene sequence:

>1092_bases
ATGGCGAAGACTTTTAAAATTGCGGTCCTGCCGGGTGATGGTATCGGACCTGAGGTGATGGCTGAGGCAACCAGGGTGCT
GGATGTAATTGGCCAGAAATACAACGTAACCTTTGAAGGCACCTTTGCCAATGTGGGTGGTGCCGGGATTGACAATGAGG
GCAAGGCACTGCCCCAGACAACCATTGATATCTGCAAGGATTCCGATGCGATCCTGTTCGGTTCGGTGGGCGGTCCCAAG
TGGGAGTCACTGCCGCCGGATGAACAACCTGAGCGTGGGGCACTGCTGCCGCTGCGCAAGATCTTCGGTCTCTACGCCAA
TCTGCGTCCGGCCATTATCTTCCCTTCTCTGACCGGCGCTTCATCGCTGAAAGAGGAAGTAATCGGCGGCGGCTTCAATG
TACTGGTGATCCGCGAGCTGACCGGCGGCATCTACTTCTCCCAGCCCAAGGGCATTGAAGGCGAAGGCCGCAACCGGATC
GGCATCGACACCATGAAGTACAGCGTGCCGGAGATCGAGCGGATCACCCATGTTGCCTTTCAGGCTGCCCGGAAGCGTGG
CAAGAAGGTCTGCTCCATTGACAAGGCCAACGTGCTCTCCTCTTCCGTGCTCTGGCGTGAAGTGGTGATTGACATTGCCA
AGCAGTACCCTGATGTGGAACTGTCCCACATGTACGTGGATAACGCTGCCATGCAGCTGGTCAAATGGCCCAAGCAGTTT
GACGTGATCCTGTGCGAAAACATGTTCGGCGATATCCTCTCTGATGAGGCTGCCATGCTGACCGGCTCCCTCGGTATGCT
CCCCTCCGCCTCGCTGGCAGAAGGCAGCTTCGGCATGTATGAGCCATCCGGCGGCTCTGCCCCGGACATCGCCGGTCAGG
GGATTGCCAACCCGATCGCCCAGATCCTGTCTGCAGCCATGATGCTCAAGTTTTCCTGCGGCATGGTTGAGGCGGCTGAC
GCCATTGACACCGCTGTGGAGACCGTACTGGATCAGGGTTACCGTACCCGTGACATCTTCCAGAACAAGTCAGGCGAGAA
GCTGGTGAATACCAAAGAGATGGGTGACGCGATAATTGCAGCACTTGCGTAA

Upstream 100 bases:

>100_bases
AAGGGGCGGTCTCCAAAGAGATCGCCCCTTTTTCGTGATGATTGACAAACATCTAATGTATACAGTAAGGTAGCGCACTT
TTTTGACAGAGGGGTGGAAC

Downstream 100 bases:

>100_bases
TGTAACTGTACTTTAATATAATCGGATTAATTGAACTGCATATATTCATTTTGAAGGAGTACATACCATGAAAGTAGGAA
TCGTTGGTTGGCGCGGTATG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAKTFKIAVLPGDGIGPEVMAEATRVLDVIGQKYNVTFEGTFANVGGAGIDNEGKALPQTTIDICKDSDAILFGSVGGPK
WESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIGGGFNVLVIRELTGGIYFSQPKGIEGEGRNRI
GIDTMKYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVIDIAKQYPDVELSHMYVDNAAMQLVKWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIAQILSAAMMLKFSCGMVEAAD
AIDTAVETVLDQGYRTRDIFQNKSGEKLVNTKEMGDAIIAALA

Sequences:

>Translated_363_residues
MAKTFKIAVLPGDGIGPEVMAEATRVLDVIGQKYNVTFEGTFANVGGAGIDNEGKALPQTTIDICKDSDAILFGSVGGPK
WESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIGGGFNVLVIRELTGGIYFSQPKGIEGEGRNRI
GIDTMKYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVIDIAKQYPDVELSHMYVDNAAMQLVKWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIAQILSAAMMLKFSCGMVEAAD
AIDTAVETVLDQGYRTRDIFQNKSGEKLVNTKEMGDAIIAALA
>Mature_362_residues
AKTFKIAVLPGDGIGPEVMAEATRVLDVIGQKYNVTFEGTFANVGGAGIDNEGKALPQTTIDICKDSDAILFGSVGGPKW
ESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIGGGFNVLVIRELTGGIYFSQPKGIEGEGRNRIG
IDTMKYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVIDIAKQYPDVELSHMYVDNAAMQLVKWPKQFD
VILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIAQILSAAMMLKFSCGMVEAADA
IDTAVETVLDQGYRTRDIFQNKSGEKLVNTKEMGDAIIAALA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=32.2857142857143, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI28178821, Length=345, Percent_Identity=28.695652173913, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178816, Length=316, Percent_Identity=29.746835443038, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4758582, Length=338, Percent_Identity=28.698224852071, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=28.1818181818182, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=171, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=59.4972067039106, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1788101, Length=369, Percent_Identity=35.230352303523, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787381, Length=399, Percent_Identity=25.31328320802, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=345, Percent_Identity=32.463768115942, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=360, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=349, Percent_Identity=30.0859598853868, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=349, Percent_Identity=30.6590257879656, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=45.5284552845528, Blast_Score=269, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=361, Percent_Identity=34.6260387811634, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=31.728045325779, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=31.4868804664723, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=34.1176470588235, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=34.1176470588235, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=31.2320916905444, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=30.4597701149425, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=30.4597701149425, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=30.4597701149425, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=30.4597701149425, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=30.635838150289, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281362242, Length=341, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648872, Length=341, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.4186046511628, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38813; Mature: 38682

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTFKIAVLPGDGIGPEVMAEATRVLDVIGQKYNVTFEGTFANVGGAGIDNEGKALPQT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCH
TIDICKDSDAILFGSVGGPKWESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIGGGFNVLVIRELTGGIYFSQPKGIEGEGRNRIGIDTMKYSVPEIERITHVAF
HHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCEECCCHHHCCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREVVIDIAKQYPDVELSHMYVDNAAMQLVKWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSAAMMLKFSCGMVEAADAIDTAVETVLDQGYRTRDIFQNKSGEKLVNTKEMGDAIIA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCHHHHHHHHHH
ALA
HCC
>Mature Secondary Structure 
AKTFKIAVLPGDGIGPEVMAEATRVLDVIGQKYNVTFEGTFANVGGAGIDNEGKALPQT
CCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCH
TIDICKDSDAILFGSVGGPKWESLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
HHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIGGGFNVLVIRELTGGIYFSQPKGIEGEGRNRIGIDTMKYSVPEIERITHVAF
HHHHHHHHCCCCEEEEEEECCCCEEECCCCCCCCCCCCEECCCHHHCCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREVVIDIAKQYPDVELSHMYVDNAAMQLVKWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGSFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSAAMMLKFSCGMVEAADAIDTAVETVLDQGYRTRDIFQNKSGEKLVNTKEMGDAIIA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHCHHHHHHHHHH
ALA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA