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The map label for this gene is prpC [H]

Identifier: 189353984

GI number: 189353984

Start: 2306895

End: 2308067

Strand: Reverse

Name: prpC [H]

Synonym: BMULJ_05237

Alternate gene names: 189353984

Gene position: 2308067-2306895 (Counterclockwise)

Preceding gene: 189353985

Following gene: 189353983

Centisome position: 93.32

GC content: 65.22

Gene sequence:

>1173_bases
ATGAGCGAGACGAAAGACGCAGCAGCACCGGCCGCCGCAGGCGCATTCAAGCCGAAGAAGTCGGTCGCGCTGTCGGGCGT
GACGGCCGGCAACACGGCGCTGTGCACGGTCGGCAAGACCGGCAACGATCTGCACTACCGCGGCTACGACATTCTCGACG
TCGCGGAATCGTGCGAGTTCGAGGAGATCGCGCACCTGCTCGTGCACGAGACGCTGCCGAACCTGACCGAACTCGCCGCC
TACAAGGCGAAGCTGCGCGCCCTGCGCGGGCTGCCGAACGCGCTGAAGGCCGTGCTCGAGCAAATCCCGGCATCGGCGCA
TCCGATGGACGTGATGCGCACCGGCGTGTCGGTGCTCGGCACCGTGCTGCCGGAGAAGGACGACCACAACCTGCCGGGCG
CACGCGATATCGCCGATCGCCTGATGGCCTCGCTCGGCTCGATGCTGCTGTACTGGTATCACTTCTCGCACAACGGCAAG
CGGATCGAAACGGAAACCGACGACGATTCGATCGGCGGCCACTTCCTGCACCTGCTGCACGGCAAGACGCCGTCGAAGTC
GTGGGTCGACGCGATGCACACGTCGCTGATCCTGTACGCGGAGCACGAGTTCAATGCGTCGACGTTCACGGGCCGCGTGA
TCGCGGGCACCGGCTCCGACATGTACTCGGCGATCACCGGCGCGATCGGCGCGCTGCGCGGGCCGAAGCACGGCGGCGCG
AACGAGGTCGCGTATGAAATCCAGAGCCGCTACCGCTCGCCGGACGAGGCGGAAGCCGATATCCGCCGCCGCGTCGAGAA
CAAGGAAGTCGTGATCGGCTTCGGTCACCCGGTCTACACGATCTCCGATCCGCGCAACAAGGTGATCAAGGGCGTCGCGC
ACAAGCTGTCGAAGGAAGCGGGCGACCTGAAGCTGTACGACATCGCCGAGCGCCTCGAATCGGTGATGTGGGACGCGAAG
AAGATGTTCCCGAACCTCGACTGGTTCAGCGCCGTGTCGTATCACATGATGGGCGTGCCGACCGCGATGTTCACGCCGCT
GTTCGTGATCTCGCGCACGTCCGGCTGGGCCGCGCACATCATCGAGCAGCGCGTCGACAACAAGATCATCCGCCCGAGCG
CGAACTACACGGGTCCGGACGATCTGCAGTTCGTGCCGATCGAGAAGCGCTGA

Upstream 100 bases:

>100_bases
ATACGAACGCAAGCTCGACGAGCTGTTTGCGCAGGGCAAGAAGTAAGCGGCCCGCGTGGCGACGACGAATCTGCCTATCT
GAATACAGGAGAACGGAAAC

Downstream 100 bases:

>100_bases
AACACCGGCCGCGCGCACCGGCGCCCGTTTCGCGGGCGGCCGGCGCGCGTCGCACGCCACCCTTCGATTTCCCCCACATG
ATGAATACCGCCTACCGCAA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSETKDAAAPAAAGAFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVAESCEFEEIAHLLVHETLPNLTELAA
YKAKLRALRGLPNALKAVLEQIPASAHPMDVMRTGVSVLGTVLPEKDDHNLPGARDIADRLMASLGSMLLYWYHFSHNGK
RIETETDDDSIGGHFLHLLHGKTPSKSWVDAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGA
NEVAYEIQSRYRSPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKGVAHKLSKEAGDLKLYDIAERLESVMWDAK
KMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWAAHIIEQRVDNKIIRPSANYTGPDDLQFVPIEKR

Sequences:

>Translated_390_residues
MSETKDAAAPAAAGAFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVAESCEFEEIAHLLVHETLPNLTELAA
YKAKLRALRGLPNALKAVLEQIPASAHPMDVMRTGVSVLGTVLPEKDDHNLPGARDIADRLMASLGSMLLYWYHFSHNGK
RIETETDDDSIGGHFLHLLHGKTPSKSWVDAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGA
NEVAYEIQSRYRSPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKGVAHKLSKEAGDLKLYDIAERLESVMWDAK
KMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWAAHIIEQRVDNKIIRPSANYTGPDDLQFVPIEKR
>Mature_389_residues
SETKDAAAPAAAGAFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVAESCEFEEIAHLLVHETLPNLTELAAY
KAKLRALRGLPNALKAVLEQIPASAHPMDVMRTGVSVLGTVLPEKDDHNLPGARDIADRLMASLGSMLLYWYHFSHNGKR
IETETDDDSIGGHFLHLLHGKTPSKSWVDAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGAN
EVAYEIQSRYRSPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKGVAHKLSKEAGDLKLYDIAERLESVMWDAKK
MFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWAAHIIEQRVDNKIIRPSANYTGPDDLQFVPIEKR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=375, Percent_Identity=77.8666666666667, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1786939, Length=369, Percent_Identity=31.4363143631436, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17555174, Length=359, Percent_Identity=23.6768802228412, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324328, Length=345, Percent_Identity=25.5072463768116, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319850, Length=370, Percent_Identity=22.7027027027027, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42794; Mature: 42663

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKDAAAPAAAGAFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVAESCEF
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCEECCCHHHHHHHCCCH
EEIAHLLVHETLPNLTELAAYKAKLRALRGLPNALKAVLEQIPASAHPMDVMRTGVSVLG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVLPEKDDHNLPGARDIADRLMASLGSMLLYWYHFSHNGKRIETETDDDSIGGHFLHLLH
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCCCCCCHHHHHHHC
GKTPSKSWVDAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGA
CCCCCHHHHHHHHHEEEEEEECCCCCCEECCEEEECCCCHHHHHHHHHHHHCCCCCCCCH
NEVAYEIQSRYRSPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKGVAHKLSKEA
HHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHCC
GDLKLYDIAERLESVMWDAKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWAAHI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCHHHHH
IEQRVDNKIIRPSANYTGPDDLQFVPIEKR
HHHHHCCCEECCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
SETKDAAAPAAAGAFKPKKSVALSGVTAGNTALCTVGKTGNDLHYRGYDILDVAESCEF
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCEECCCHHHHHHHCCCH
EEIAHLLVHETLPNLTELAAYKAKLRALRGLPNALKAVLEQIPASAHPMDVMRTGVSVLG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVLPEKDDHNLPGARDIADRLMASLGSMLLYWYHFSHNGKRIETETDDDSIGGHFLHLLH
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCCCCCCHHHHHHHC
GKTPSKSWVDAMHTSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGPKHGGA
CCCCCHHHHHHHHHEEEEEEECCCCCCEECCEEEECCCCHHHHHHHHHHHHCCCCCCCCH
NEVAYEIQSRYRSPDEAEADIRRRVENKEVVIGFGHPVYTISDPRNKVIKGVAHKLSKEA
HHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCEEEEECCHHHHHHHHHHHHHHHCC
GDLKLYDIAERLESVMWDAKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWAAHI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHEEECCCCHHHHH
IEQRVDNKIIRPSANYTGPDDLQFVPIEKR
HHHHHCCCEECCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]