Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 189353985

GI number: 189353985

Start: 2308122

End: 2309015

Strand: Reverse

Name: prpB [H]

Synonym: BMULJ_05238

Alternate gene names: 189353985

Gene position: 2309015-2308122 (Counterclockwise)

Preceding gene: 189353987

Following gene: 189353984

Centisome position: 93.36

GC content: 66.33

Gene sequence:

>894_bases
ATGAGCAACACGCATCTTCAGAGCGCCGGCGCGAAGTTCCGCGCGGCAGTCGCGGCCGAGCAGCCGCTGCAGGTGGTCGG
CGCGATCACCGCGTACGCGGCCAAGATGGCGCAGGCCGTCGGCTTCAAGGCCGTCTATCTGTCGGGCGGCGGTGTCGCCG
CGAACTCGCTCGGCATTCCCGATCTCGGCATCAGCACGATGGAAGACGTGCTGATCGATGCGAACCGGATCACGAACGCG
ACCGACCTGCCGCTGCTCGTCGACATCGACACCGGCTGGGGCGGCGCCTTCAACATCGCGCGCACGATCCGCTCGTTCAT
CAACGCGGGCGTCGCGGCCGTGCATATCGAGGACCAGGTCGGCCAGAAGCGCTGCGGGCACCGTCCGGGCAAGGAGTGCG
TGCCGACCGACGAGATGGTCGATCGCATCAAGGCTGCCGTCGACGCGCGTACCGACGACCAGTTCGTGATCATGGCGCGC
ACCGACGCGGCCGCGGCCGAAGGCATCGATTCGGCGATCGAACGCGCGGTCGCCTACGTCGAAGCCGGCGCCGACATGAT
CTTCCCCGAGGCGATGAAGACGCTCGACGACTACCGCCGCTTCAAGGCCGCGGTGAAGGTGCCGATCCTCGCGAACCTGA
CGGAATTCGGCTCGACGCCGCTGTTCACGGTCGACGAACTGCGCGAGGCGAACGTCGACATCGCGCTGTACTGCTGCGGC
GCGTATCGCGCGATGAACAAGGCCGCGCTGAATTTCTACGAAACGGTGCGCCGCGACGGCACGCAGAAGGCGGCCGTGCC
GACGATGCAGACGCGCGCCGAGCTGTACGACTTCCTCGGCTATCACGAATACGAACGCAAGCTCGACGAGCTGTTTGCGC
AGGGCAAGAAGTAA

Upstream 100 bases:

>100_bases
CGGCTGCGGCGCCGTTCGCTTAAGAATCAGCGGGTTAGCGCGCCGGAACGACGCTGGCCCGGTCCTTGCTGTCTATGGGC
ATTCCTTCAGGAGGCCGTTC

Downstream 100 bases:

>100_bases
GCGGCCCGCGTGGCGACGACGAATCTGCCTATCTGAATACAGGAGAACGGAAACATGAGCGAGACGAAAGACGCAGCAGC
ACCGGCCGCCGCAGGCGCAT

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSNTHLQSAGAKFRAAVAAEQPLQVVGAITAYAAKMAQAVGFKAVYLSGGGVAANSLGIPDLGISTMEDVLIDANRITNA
TDLPLLVDIDTGWGGAFNIARTIRSFINAGVAAVHIEDQVGQKRCGHRPGKECVPTDEMVDRIKAAVDARTDDQFVIMAR
TDAAAAEGIDSAIERAVAYVEAGADMIFPEAMKTLDDYRRFKAAVKVPILANLTEFGSTPLFTVDELREANVDIALYCCG
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYDFLGYHEYERKLDELFAQGKK

Sequences:

>Translated_297_residues
MSNTHLQSAGAKFRAAVAAEQPLQVVGAITAYAAKMAQAVGFKAVYLSGGGVAANSLGIPDLGISTMEDVLIDANRITNA
TDLPLLVDIDTGWGGAFNIARTIRSFINAGVAAVHIEDQVGQKRCGHRPGKECVPTDEMVDRIKAAVDARTDDQFVIMAR
TDAAAAEGIDSAIERAVAYVEAGADMIFPEAMKTLDDYRRFKAAVKVPILANLTEFGSTPLFTVDELREANVDIALYCCG
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYDFLGYHEYERKLDELFAQGKK
>Mature_296_residues
SNTHLQSAGAKFRAAVAAEQPLQVVGAITAYAAKMAQAVGFKAVYLSGGGVAANSLGIPDLGISTMEDVLIDANRITNAT
DLPLLVDIDTGWGGAFNIARTIRSFINAGVAAVHIEDQVGQKRCGHRPGKECVPTDEMVDRIKAAVDARTDDQFVIMART
DAAAAEGIDSAIERAVAYVEAGADMIFPEAMKTLDDYRRFKAAVKVPILANLTEFGSTPLFTVDELREANVDIALYCCGA
YRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYDFLGYHEYERKLDELFAQGKK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=292, Percent_Identity=63.3561643835616, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1790445, Length=258, Percent_Identity=29.4573643410853, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982926, Length=224, Percent_Identity=30.3571428571429, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17561814, Length=224, Percent_Identity=30.3571428571429, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320908, Length=105, Percent_Identity=36.1904761904762, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32095; Mature: 31964

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTHLQSAGAKFRAAVAAEQPLQVVGAITAYAAKMAQAVGFKAVYLSGGGVAANSLGIP
CCCCCCHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCC
DLGISTMEDVLIDANRITNATDLPLLVDIDTGWGGAFNIARTIRSFINAGVAAVHIEDQV
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHH
GQKRCGHRPGKECVPTDEMVDRIKAAVDARTDDQFVIMARTDAAAAEGIDSAIERAVAYV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAMKTLDDYRRFKAAVKVPILANLTEFGSTPLFTVDELREANVDIALYCCG
HCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCCCEEEEECH
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYDFLGYHEYERKLDELFAQGKK
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNTHLQSAGAKFRAAVAAEQPLQVVGAITAYAAKMAQAVGFKAVYLSGGGVAANSLGIP
CCCCCHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCC
DLGISTMEDVLIDANRITNATDLPLLVDIDTGWGGAFNIARTIRSFINAGVAAVHIEDQV
CCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEHHHH
GQKRCGHRPGKECVPTDEMVDRIKAAVDARTDDQFVIMARTDAAAAEGIDSAIERAVAYV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAMKTLDDYRRFKAAVKVPILANLTEFGSTPLFTVDELREANVDIALYCCG
HCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHCCCCEEEEECH
AYRAMNKAALNFYETVRRDGTQKAAVPTMQTRAELYDFLGYHEYERKLDELFAQGKK
HHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]