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The map label for this gene is dctD [H]

Identifier: 189353886

GI number: 189353886

Start: 2195668

End: 2197029

Strand: Direct

Name: dctD [H]

Synonym: BMULJ_05138

Alternate gene names: 189353886

Gene position: 2195668-2197029 (Clockwise)

Preceding gene: 189353885

Following gene: 189353887

Centisome position: 88.78

GC content: 67.77

Gene sequence:

>1362_bases
ATGGAAGATGAGATTCGCGTGCTGGTCGTCGAGGACGATGAAAACGTCCGGATCGGCGTCGAGCAGGCTGTTGCGCTCGC
CGGGTTTCCTGTGCAGGCGTTCGCGTCGGCAGCCGACGCGCTCGCGCACGTCGCGCCCGGCGCGCCGGTCGTCATCGTGT
CCGACGTGCGGATGCCCGGCATCGACGGGCTGCAGCTGCTCGAACGCGTGATGGCCGTCGATTCGCAGATTCCGGTCGTG
CTGATCAGCGGTCACGCCGACATCTCGACGGCCGTCGGCGCGATGCACGACGGCGCGTACGACTTCATCGAGAAGCCGTT
TTCGTCGGACCTGATCGCGGGCCGCGTCGCGCGCGCGGTCGAGAAGCGCCGGCTCACGCTCGAAGTGCAAGGGCTGCGCG
CGGCGCTGCACAACTGGCAGGGAATCGAAGCGCTCGTGCTCGGCAAGTCGCCCGCGATGGCCGACGTGCGCAAGAAAATC
CTACGCCTCGCCGATACGTCGGTGTCGGTGCTGATCACCGGCGAGACCGGCACCGGCAAGGAGCTGATCGCACGCAGCCT
GCACGACTTCGGCGGCCGGCGCGATGCGCATTTCGTCGCGCTGAACTGCGGCGGGCTGCCCGAACAGATCTTCGAGAGCG
AACTGTTCGGCCACGAGGCCGGCGCGTTCACGGGCGCGATCAAGAAGCGCATCGGCAAGATCGAATGGGCGCAGGGCGGC
ACGCTATTTCTCGACGAGATCGAGACGATGCCGATTCCGCTGCAGATCAAGATGCTGCGCGTGCTGCAGGAGCGCGTCGT
CGAGCGGCTCGGTGCGAACGAGCTGATTCCGGTCGACTGCCGCGTGATCGCCGCGTCGAAGGCCGATCTCGCCGAACTCG
CCGCGGACGGACGGTTCCGCGCCGATCTGCTGTACCGCCTGAACGTCGCGCAGATCGAATTGCCGCCGCTGCGCGAGCGG
CGCGAGGACGTGCCGCTGCTGTTCGAGCATTTCGTGCTCGCCGCCGCGCGGCGCTTCGGACAGCCGGCGCCTGTCGTATC
GGCGGCTCAGGTATCCGAACTGATGACACATGCGTGGCCCGGCAATGTACGCGAACTGCAGAACGTGGCCGATCGCTTCG
TGCTCGGGCTGACCGGCGACAGCCTGCTGTCGGGCGACGGCCCGCGCCCGACGAGCGGCACGCTCGCCGAACAGCTCGCG
TATTTCGAGCGGATGCTGATCGAGGACATGCTGCGGCGTCACCACGGCAACGTCGCGGACGCGAGCATCGCGCTCGGGAT
GCCGAAGAAGACGCTGTACCACAAGCTGCGCCACCTGCGGATTCCCGCGCGCGGCGACACGGCGCCTGACGGCGGCGAAT
GA

Upstream 100 bases:

>100_bases
GTCGAAGGATTCGGCGCGAAGATCGCCGCGACGAACCGTGACGGCGGCGGTGCGCAGTTCACCATCGAATTCGCGGCGGC
CACGCCGCAAAGGACAGAGC

Downstream 100 bases:

>100_bases
CGCGAACGAGGACACGCACCGGCATGGATCGCATCGACATCACCGAACACGGCCGCCTCGACGGCTTCCTGATCCGCGGC
ATCACGCACGCGCAGAAAAC

Product: Fis family two-component sigma54-dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MEDEIRVLVVEDDENVRIGVEQAVALAGFPVQAFASAADALAHVAPGAPVVIVSDVRMPGIDGLQLLERVMAVDSQIPVV
LISGHADISTAVGAMHDGAYDFIEKPFSSDLIAGRVARAVEKRRLTLEVQGLRAALHNWQGIEALVLGKSPAMADVRKKI
LRLADTSVSVLITGETGTGKELIARSLHDFGGRRDAHFVALNCGGLPEQIFESELFGHEAGAFTGAIKKRIGKIEWAQGG
TLFLDEIETMPIPLQIKMLRVLQERVVERLGANELIPVDCRVIAASKADLAELAADGRFRADLLYRLNVAQIELPPLRER
REDVPLLFEHFVLAAARRFGQPAPVVSAAQVSELMTHAWPGNVRELQNVADRFVLGLTGDSLLSGDGPRPTSGTLAEQLA
YFERMLIEDMLRRHHGNVADASIALGMPKKTLYHKLRHLRIPARGDTAPDGGE

Sequences:

>Translated_453_residues
MEDEIRVLVVEDDENVRIGVEQAVALAGFPVQAFASAADALAHVAPGAPVVIVSDVRMPGIDGLQLLERVMAVDSQIPVV
LISGHADISTAVGAMHDGAYDFIEKPFSSDLIAGRVARAVEKRRLTLEVQGLRAALHNWQGIEALVLGKSPAMADVRKKI
LRLADTSVSVLITGETGTGKELIARSLHDFGGRRDAHFVALNCGGLPEQIFESELFGHEAGAFTGAIKKRIGKIEWAQGG
TLFLDEIETMPIPLQIKMLRVLQERVVERLGANELIPVDCRVIAASKADLAELAADGRFRADLLYRLNVAQIELPPLRER
REDVPLLFEHFVLAAARRFGQPAPVVSAAQVSELMTHAWPGNVRELQNVADRFVLGLTGDSLLSGDGPRPTSGTLAEQLA
YFERMLIEDMLRRHHGNVADASIALGMPKKTLYHKLRHLRIPARGDTAPDGGE
>Mature_453_residues
MEDEIRVLVVEDDENVRIGVEQAVALAGFPVQAFASAADALAHVAPGAPVVIVSDVRMPGIDGLQLLERVMAVDSQIPVV
LISGHADISTAVGAMHDGAYDFIEKPFSSDLIAGRVARAVEKRRLTLEVQGLRAALHNWQGIEALVLGKSPAMADVRKKI
LRLADTSVSVLITGETGTGKELIARSLHDFGGRRDAHFVALNCGGLPEQIFESELFGHEAGAFTGAIKKRIGKIEWAQGG
TLFLDEIETMPIPLQIKMLRVLQERVVERLGANELIPVDCRVIAASKADLAELAADGRFRADLLYRLNVAQIELPPLRER
REDVPLLFEHFVLAAARRFGQPAPVVSAAQVSELMTHAWPGNVRELQNVADRFVLGLTGDSLLSGDGPRPTSGTLAEQLA
YFERMLIEDMLRRHHGNVADASIALGMPKKTLYHKLRHLRIPARGDTAPDGGE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=449, Percent_Identity=40.0890868596882, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=39.6995708154506, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790437, Length=443, Percent_Identity=38.1489841986456, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1788905, Length=440, Percent_Identity=38.4090909090909, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1789087, Length=352, Percent_Identity=43.75, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=44.3076923076923, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=41.4330218068536, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=41.5873015873016, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87082152, Length=230, Percent_Identity=48.2608695652174, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=45.2173913043478, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=305, Percent_Identity=37.3770491803279, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=31.5068493150685, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789828, Length=326, Percent_Identity=29.1411042944785, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082012, Length=132, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49170; Mature: 49170

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDEIRVLVVEDDENVRIGVEQAVALAGFPVQAFASAADALAHVAPGAPVVIVSDVRMPG
CCCCEEEEEEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
IDGLQLLERVMAVDSQIPVVLISGHADISTAVGAMHDGAYDFIEKPFSSDLIAGRVARAV
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHH
EKRRLTLEVQGLRAALHNWQGIEALVLGKSPAMADVRKKILRLADTSVSVLITGETGTGK
HHHHEEEEEHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCH
ELIARSLHDFGGRRDAHFVALNCGGLPEQIFESELFGHEAGAFTGAIKKRIGKIEWAQGG
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCC
TLFLDEIETMPIPLQIKMLRVLQERVVERLGANELIPVDCRVIAASKADLAELAADGRFR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCHHHHHHHCCCCEE
ADLLYRLNVAQIELPPLRERREDVPLLFEHFVLAAARRFGQPAPVVSAAQVSELMTHAWP
EEHHEEECEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GNVRELQNVADRFVLGLTGDSLLSGDGPRPTSGTLAEQLAYFERMLIEDMLRRHHGNVAD
CCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASIALGMPKKTLYHKLRHLRIPARGDTAPDGGE
CEEEECCCHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure
MEDEIRVLVVEDDENVRIGVEQAVALAGFPVQAFASAADALAHVAPGAPVVIVSDVRMPG
CCCCEEEEEEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
IDGLQLLERVMAVDSQIPVVLISGHADISTAVGAMHDGAYDFIEKPFSSDLIAGRVARAV
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHH
EKRRLTLEVQGLRAALHNWQGIEALVLGKSPAMADVRKKILRLADTSVSVLITGETGTGK
HHHHEEEEEHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCH
ELIARSLHDFGGRRDAHFVALNCGGLPEQIFESELFGHEAGAFTGAIKKRIGKIEWAQGG
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEEECCCC
TLFLDEIETMPIPLQIKMLRVLQERVVERLGANELIPVDCRVIAASKADLAELAADGRFR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCHHHHHHHCCCCEE
ADLLYRLNVAQIELPPLRERREDVPLLFEHFVLAAARRFGQPAPVVSAAQVSELMTHAWP
EEHHEEECEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
GNVRELQNVADRFVLGLTGDSLLSGDGPRPTSGTLAEQLAYFERMLIEDMLRRHHGNVAD
CCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASIALGMPKKTLYHKLRHLRIPARGDTAPDGGE
CEEEECCCHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]