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The map label for this gene is proP [H]

Identifier: 189353863

GI number: 189353863

Start: 2169230

End: 2170540

Strand: Direct

Name: proP [H]

Synonym: BMULJ_05115

Alternate gene names: 189353863

Gene position: 2169230-2170540 (Clockwise)

Preceding gene: 189353862

Following gene: 189353868

Centisome position: 87.71

GC content: 67.66

Gene sequence:

>1311_bases
ATGCAAGCTTCCGAACTGAGCGGCGTGCCTCGCGCCGCCGAACGCGCGCTCACGCGCAGCGACTACAAAACCCTTGGCCT
CGCCGCGCTCGGCGGCGCGCTCGAGTTCTACGACTTCATCATCTTCGTGTTCTTCGCGCCCGCGATCGGCCAGCTGTTCT
TCCCGCACGACATTCCCGACTGGCTGCGCCAACTGCAGACGTTCGGCATCTTCGCGGCCGGCTATCTTGCGCGCCCGCTC
GGCGGCGTGATCATGGCGCACTTCGGCGACCTCGTCGGCCGCAAGCGGATGTTTACGCTCAGCGTGCTGCTGATGTCGGT
GCCGACGCTGCTGATGGGCCTGCTGCCGACCTACGACACGATCGGCATCCTCGCGCCGGTGCTCCTGCTGCTGTTCCGCA
TCCTGCAGGGCGCGGCCGTCGGCGGCGAAGTGCCGGGTGCGTGGGTGTTCGTGTCCGAGCACGTGCCGTCGCGGCACATC
GGCTATGCGTGCGGCACGCTGACTGCCGGGCTGACGGCCGGCATCCTGCTCGGCTCGCTCGTCGCCGCCGGCGTGAACAG
CCGCTATTCGACCGCCGAAGTCGCCGCGTTCGCGTGGCGCATTCCGTTCCTGCTCGGCGGTGTGTTCGGGCTGTTCTCCG
TGTATCTGCGCCGCTGGCTGCACGAAACGCCGGTGTTCGCCGAGATGAAGGCGAAGAAGGCGCTTGCCGCCGAGATTCCG
CTGAAGGCCGTGCTGCGCGATCACGGCCGCGCGGTGATCGTGTCGATGCTGCTCACGTGGATGCTGTCCGCGGCGATCGT
CGTCGTGATCCTGATGACGCCCGCGCTGCTGCAGAAGCAGTTTCACCTGACGCCCGCGACCACGCTGTTCGCGAACAGCG
TCGCGACGCTGTGCCTGACGGCCGGCTGCATCAGCGCCGGCTCGCTCGCCGGCTGGTTCGGCGCGAAGCGCGTGCTCGGC
ATCGGCGGCCTCGTGCTCGCCGCGTGCTACTACCTGCTTTTCGTGCAGGTCGCCGCCGACGCGTCGACGCTGCCGCTGTA
CTACGGGATCGCGGGTTTCACGGTCGGCACGATCGGCGCGGTGCCGTTCGTGATGGTGAAGAGCTTCCCGCCGGTCGTGC
GCTTCTCGGGCATCTCGTTCTCGTACAACGTCGCGTATGCGATCTTCGGCGGCCTGACGCCGGTGATCGTGTCGCTGATG
ATGAAGTGGAACCCGATGGCGCCGGTCGTCTATGTCGCGGCGATCTGCGTGCTCGGCGCGATCGCCGTGCAATTCGCGAA
GGATGCAAAGGACGCGACGGCCGCGCGCTGA

Upstream 100 bases:

>100_bases
TTCCGACGACGCGGGCCGCGGCCGTGCGCCTGTTCACGCCAACAGCGGGCCCTGGAGACGCAGCACCGACAACTACATTC
GAAAACCGATCGTCCTGACC

Downstream 100 bases:

>100_bases
GCGCGCGCCCAGGCGCCCGACGAACGAAGGGCCGAATTCCGCAGGCGGCGGAATTCGGCCCTTTCGTTTTTGCGCCCGCG
CGGTGGTGCAGCGGCGCGGT

Product: permease of the major facilitator superfamily

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQASELSGVPRAAERALTRSDYKTLGLAALGGALEFYDFIIFVFFAPAIGQLFFPHDIPDWLRQLQTFGIFAAGYLARPL
GGVIMAHFGDLVGRKRMFTLSVLLMSVPTLLMGLLPTYDTIGILAPVLLLLFRILQGAAVGGEVPGAWVFVSEHVPSRHI
GYACGTLTAGLTAGILLGSLVAAGVNSRYSTAEVAAFAWRIPFLLGGVFGLFSVYLRRWLHETPVFAEMKAKKALAAEIP
LKAVLRDHGRAVIVSMLLTWMLSAAIVVVILMTPALLQKQFHLTPATTLFANSVATLCLTAGCISAGSLAGWFGAKRVLG
IGGLVLAACYYLLFVQVAADASTLPLYYGIAGFTVGTIGAVPFVMVKSFPPVVRFSGISFSYNVAYAIFGGLTPVIVSLM
MKWNPMAPVVYVAAICVLGAIAVQFAKDAKDATAAR

Sequences:

>Translated_436_residues
MQASELSGVPRAAERALTRSDYKTLGLAALGGALEFYDFIIFVFFAPAIGQLFFPHDIPDWLRQLQTFGIFAAGYLARPL
GGVIMAHFGDLVGRKRMFTLSVLLMSVPTLLMGLLPTYDTIGILAPVLLLLFRILQGAAVGGEVPGAWVFVSEHVPSRHI
GYACGTLTAGLTAGILLGSLVAAGVNSRYSTAEVAAFAWRIPFLLGGVFGLFSVYLRRWLHETPVFAEMKAKKALAAEIP
LKAVLRDHGRAVIVSMLLTWMLSAAIVVVILMTPALLQKQFHLTPATTLFANSVATLCLTAGCISAGSLAGWFGAKRVLG
IGGLVLAACYYLLFVQVAADASTLPLYYGIAGFTVGTIGAVPFVMVKSFPPVVRFSGISFSYNVAYAIFGGLTPVIVSLM
MKWNPMAPVVYVAAICVLGAIAVQFAKDAKDATAAR
>Mature_436_residues
MQASELSGVPRAAERALTRSDYKTLGLAALGGALEFYDFIIFVFFAPAIGQLFFPHDIPDWLRQLQTFGIFAAGYLARPL
GGVIMAHFGDLVGRKRMFTLSVLLMSVPTLLMGLLPTYDTIGILAPVLLLLFRILQGAAVGGEVPGAWVFVSEHVPSRHI
GYACGTLTAGLTAGILLGSLVAAGVNSRYSTAEVAAFAWRIPFLLGGVFGLFSVYLRRWLHETPVFAEMKAKKALAAEIP
LKAVLRDHGRAVIVSMLLTWMLSAAIVVVILMTPALLQKQFHLTPATTLFANSVATLCLTAGCISAGSLAGWFGAKRVLG
IGGLVLAACYYLLFVQVAADASTLPLYYGIAGFTVGTIGAVPFVMVKSFPPVVRFSGISFSYNVAYAIFGGLTPVIVSLM
MKWNPMAPVVYVAAICVLGAIAVQFAKDAKDATAAR

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=28.436018957346, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788292, Length=446, Percent_Identity=30.9417040358744, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=453, Percent_Identity=30.0220750551876, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788942, Length=397, Percent_Identity=31.7380352644836, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082231, Length=454, Percent_Identity=24.2290748898678, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46378; Mature: 46378

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHCCCCCCCCC
>Mature Secondary Structure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HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]