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The map label for this gene is metR [H]

Identifier: 189353836

GI number: 189353836

Start: 2140604

End: 2141512

Strand: Direct

Name: metR [H]

Synonym: BMULJ_05083

Alternate gene names: 189353836

Gene position: 2140604-2141512 (Clockwise)

Preceding gene: 189353834

Following gene: 189353837

Centisome position: 86.55

GC content: 63.92

Gene sequence:

>909_bases
ATGCTCGAACGTTTTCATCTCGTCGTCATTCGCGAAGTCGAACGGCAAGGCTCGCTGACCGCCGCCGCCAACGCGCTGCA
TCTGACGCAATCGGCCTTGAGCCATACCGTCAAGAAGCTCGAGCAGCAGCTCGGCACGCCGATCTGGTACCGCGAAGGCC
GCGGGCTGCGGCTCACGCAGGGCGGTCAGTATCTGCTGCGGCTTGCGCATCGGCTGCTGCCGCAGTTCGAGCTCGCCGAG
GAACGGATGAAGCAGTATGCGAAAGGCGAGCGCGGCACGCTGCGCATCGGGATGGAATGCCACCCGTGCTATCAGTGGCT
GCTGAAGGTCGTGTCGCCGTACCTGTCGCGCTGGCCGGACGTCGACGTCGATGTGAAGCAACGCTTCCAGTTCGGCGGAA
TCGGCGCGCTGTTCGGCTACGACATCGACGTGCTCGTGACGCCCGATCCGCTGAACAAACCGGGGCTGCGATTCGATCCG
GTATTCGACTACGAGCAGGTGCTGGTCGTCGCGGACGCGCACCGTTTCGCGCGCGCCGACTACGTGACGCCCGAACAACT
CGTCGACGAGGTGTTGATCACGTATCCGGTCGAAACCGACCGGCTCGATATCTACAACCAGTTCCTGACGCCGGCCGGCA
TCGTGCCGAGGCGGCACAAGTCGATCGAGACGACCGACATCATGCTGCAGATGGTCGCGAGCGGACGCGGCGTCGCCGCG
CTGCCGAGATGGCTCGCCGACGAATATGCAGACCGGATGCCGGTCGTGCCGGTCAAGCTCGGCAAAAAAGGGATCGCGAA
ACAGATCTTTCTCGGCATCCGCGAAGCGGACGCGCAGATCGACTATCTCGCCGCGTTCGTCGCGCTCGCACGCGAATCGA
CGTGGAACGCGCCGCGCCTGTTGAGGTAA

Upstream 100 bases:

>100_bases
GATCGGCAGCCATGATAGGGATTTCAACACATGAAATAAAATGGCATTATTTCATTCATCCATCAAATCTGTTCATGTAT
CCGGTTCGCCGGCGCACGCC

Downstream 100 bases:

>100_bases
GCCGCGCGCGGCGGCCGGGCCCGCTGTCATCAAACTGACTGAATGGCGACGCTAGGATCGGACGGGACTCCTTCACGAGA
TGCCGCCATGAACCAGCCTG

Product: LysR family regulator for metE and metH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MLERFHLVVIREVERQGSLTAAANALHLTQSALSHTVKKLEQQLGTPIWYREGRGLRLTQGGQYLLRLAHRLLPQFELAE
ERMKQYAKGERGTLRIGMECHPCYQWLLKVVSPYLSRWPDVDVDVKQRFQFGGIGALFGYDIDVLVTPDPLNKPGLRFDP
VFDYEQVLVVADAHRFARADYVTPEQLVDEVLITYPVETDRLDIYNQFLTPAGIVPRRHKSIETTDIMLQMVASGRGVAA
LPRWLADEYADRMPVVPVKLGKKGIAKQIFLGIREADAQIDYLAAFVALARESTWNAPRLLR

Sequences:

>Translated_302_residues
MLERFHLVVIREVERQGSLTAAANALHLTQSALSHTVKKLEQQLGTPIWYREGRGLRLTQGGQYLLRLAHRLLPQFELAE
ERMKQYAKGERGTLRIGMECHPCYQWLLKVVSPYLSRWPDVDVDVKQRFQFGGIGALFGYDIDVLVTPDPLNKPGLRFDP
VFDYEQVLVVADAHRFARADYVTPEQLVDEVLITYPVETDRLDIYNQFLTPAGIVPRRHKSIETTDIMLQMVASGRGVAA
LPRWLADEYADRMPVVPVKLGKKGIAKQIFLGIREADAQIDYLAAFVALARESTWNAPRLLR
>Mature_302_residues
MLERFHLVVIREVERQGSLTAAANALHLTQSALSHTVKKLEQQLGTPIWYREGRGLRLTQGGQYLLRLAHRLLPQFELAE
ERMKQYAKGERGTLRIGMECHPCYQWLLKVVSPYLSRWPDVDVDVKQRFQFGGIGALFGYDIDVLVTPDPLNKPGLRFDP
VFDYEQVLVVADAHRFARADYVTPEQLVDEVLITYPVETDRLDIYNQFLTPAGIVPRRHKSIETTDIMLQMVASGRGVAA
LPRWLADEYADRMPVVPVKLGKKGIAKQIFLGIREADAQIDYLAAFVALARESTWNAPRLLR

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=292, Percent_Identity=38.3561643835616, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=26.9076305220884, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI2367136, Length=256, Percent_Identity=26.5625, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=23.9057239057239, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=23.9837398373984, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787949, Length=99, Percent_Identity=34.3434343434343, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=26.0416666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788296, Length=291, Percent_Identity=25.4295532646048, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=257, Percent_Identity=26.0700389105058, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=25.7028112449799, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082024, Length=278, Percent_Identity=23.7410071942446, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=114, Percent_Identity=29.8245614035088, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34394; Mature: 34394

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERFHLVVIREVERQGSLTAAANALHLTQSALSHTVKKLEQQLGTPIWYREGRGLRLTQ
CCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECC
GGQYLLRLAHRLLPQFELAEERMKQYAKGERGTLRIGMECHPCYQWLLKVVSPYLSRWPD
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
VDVDVKQRFQFGGIGALFGYDIDVLVTPDPLNKPGLRFDPVFDYEQVLVVADAHRFARAD
CCCCHHHHHCCCCEEEHCCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEEECHHHHHHCC
YVTPEQLVDEVLITYPVETDRLDIYNQFLTPAGIVPRRHKSIETTDIMLQMVASGRGVAA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
LPRWLADEYADRMPVVPVKLGKKGIAKQIFLGIREADAQIDYLAAFVALARESTWNAPRL
HHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LR
CC
>Mature Secondary Structure
MLERFHLVVIREVERQGSLTAAANALHLTQSALSHTVKKLEQQLGTPIWYREGRGLRLTQ
CCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECC
GGQYLLRLAHRLLPQFELAEERMKQYAKGERGTLRIGMECHPCYQWLLKVVSPYLSRWPD
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
VDVDVKQRFQFGGIGALFGYDIDVLVTPDPLNKPGLRFDPVFDYEQVLVVADAHRFARAD
CCCCHHHHHCCCCEEEHCCCCEEEEECCCCCCCCCCCCCCCCCHHHEEEEECHHHHHHCC
YVTPEQLVDEVLITYPVETDRLDIYNQFLTPAGIVPRRHKSIETTDIMLQMVASGRGVAA
CCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
LPRWLADEYADRMPVVPVKLGKKGIAKQIFLGIREADAQIDYLAAFVALARESTWNAPRL
HHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]