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The map label for this gene is shiA [H]

Identifier: 189353834

GI number: 189353834

Start: 2136783

End: 2138123

Strand: Direct

Name: shiA [H]

Synonym: BMULJ_05081

Alternate gene names: 189353834

Gene position: 2136783-2138123 (Clockwise)

Preceding gene: 189353833

Following gene: 189353836

Centisome position: 86.4

GC content: 67.79

Gene sequence:

>1341_bases
ATGGAGACTTCCGTCAAGCAACCGAAGCGGGCGGCGCTTGCGTCGTTCGTCGGCACGACGATCGAGTGGTACGACTTCTA
CAGCTATGCGACCGCCGCCGCCATCGTGTTCGGCCCGCTGTTCTTTCCGGGCGAGAACCGCTTCGTGAGCTTGCTCGCGT
CGTTCGGCTCGTTCGCGGTGGGCTTTTTCGCGCGGCCGTTCGGCGGCGTGCTGTTCGGCTATCTCGGCGACCGCTTCGGC
CGCAAGCGCTCGCTGCTCGCGACGCTGATGCTGATGGCCGTGTCGACGGTCGCGATCGGCCTGCTGCCCACCTATGCGAC
GGCCGGCATGCTCGCGCCGATCCTGCTCGTGCTGATGCGCGTGCTGCAAGGGATCGCGGTCGGCGGCGAATGGGGCGGCG
CGGTGCTGCTCGCCGGCGAACATGCGCCGGCAGGCAAGCGCACGTTCTTCGCCTCGTTCGCGCAGCTCGGCAGCGCGAGC
GGCCTGATCCTGTCGATGCTCGCGTTCGGCGCGATCAGCACGCTGCCGAAGGAAGACCTGATGAGCTGGGGCTGGCGCCT
GCCGTTCCTCGCGAGCGCGGTACTGCTCGCCGTCGGCTTCGTGATTCGCGCGAGCGTGTCCGAATCGCCGGAGTTCGAGG
AAGTGCGCAAGAGCGGCGACACGGCGCGCAATCCGGTGCGCGAGGCGCTGAAGTACTGGCCGCTGCTGCTGCTCGCGATC
GGTGCGAACGTGTACGGGATCGCCGGCGTGTACTTCAGCAACATCTTCATGATCAGCTATGCGACGCAGTTCCTGTCGCT
GGACCGCTCGATGGTGCTGCATTGCATGACGATCGTGGCCGTGCTGCAGTTCGTCGTGCAGCTCGCGGCCGCGTTCTTCG
CGCAGCGCTTCGGAACGACGCGCGTGCTGCTGATCACCGGCGCGTGGGCCGCGATCGTGCCGTTCGTGATGCTGCCGCTC
GTGCATGTGGGCACGCCGCTGTCGATCACGGTCGGCGTCGGCCTCGCGACGCTCGCGGAATCGGGCTACTACTCGGTCGT
CGCGGGCTTCGTGAGCGGCATCTTCGTCGCACGGATCCGCTATACGGCGATCTCGATCGCCTATCAGGTGTGCGGCGCGC
TCGCCGGCGGCCTGACGCCGCTCGTCGCGACGATCATCGCGCAGAACACCGCGCCGCAGTGGTGGCCGCTCGCGATTCAA
TACACGAGTGCTGCGGTGCTGTCATCGCTGTGCGTCTGGCTGATCTCGCGCCGCGTCAGCATCGACGATGCGGGATCGTC
CGCGAAGGACGTCGATGCGCTGCCGCGCGCCGGCGCGCACACCGCCGCGCGTACGGCGTAA

Upstream 100 bases:

>100_bases
CGCATACGAGCTGCTGTGCGTGCTGCCGGGCGTCGCGTACCGCGACTGACGCGCGCCACGACCATTCCCAATCAGAGGAT
TCATGAGGAGCCAGAAAAAG

Downstream 100 bases:

>100_bases
AACGTCGCACTTCGCCGGGCAGACCTCAGCGCCTGCCCGGCGCGCCGCGCGCGGCAACGTTACTGCTGCTGCGCGCGCAG
CGTCTTCGCGGCCGCCACCA

Product: permease of the major facilitator superfamily

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
METSVKQPKRAALASFVGTTIEWYDFYSYATAAAIVFGPLFFPGENRFVSLLASFGSFAVGFFARPFGGVLFGYLGDRFG
RKRSLLATLMLMAVSTVAIGLLPTYATAGMLAPILLVLMRVLQGIAVGGEWGGAVLLAGEHAPAGKRTFFASFAQLGSAS
GLILSMLAFGAISTLPKEDLMSWGWRLPFLASAVLLAVGFVIRASVSESPEFEEVRKSGDTARNPVREALKYWPLLLLAI
GANVYGIAGVYFSNIFMISYATQFLSLDRSMVLHCMTIVAVLQFVVQLAAAFFAQRFGTTRVLLITGAWAAIVPFVMLPL
VHVGTPLSITVGVGLATLAESGYYSVVAGFVSGIFVARIRYTAISIAYQVCGALAGGLTPLVATIIAQNTAPQWWPLAIQ
YTSAAVLSSLCVWLISRRVSIDDAGSSAKDVDALPRAGAHTAARTA

Sequences:

>Translated_446_residues
METSVKQPKRAALASFVGTTIEWYDFYSYATAAAIVFGPLFFPGENRFVSLLASFGSFAVGFFARPFGGVLFGYLGDRFG
RKRSLLATLMLMAVSTVAIGLLPTYATAGMLAPILLVLMRVLQGIAVGGEWGGAVLLAGEHAPAGKRTFFASFAQLGSAS
GLILSMLAFGAISTLPKEDLMSWGWRLPFLASAVLLAVGFVIRASVSESPEFEEVRKSGDTARNPVREALKYWPLLLLAI
GANVYGIAGVYFSNIFMISYATQFLSLDRSMVLHCMTIVAVLQFVVQLAAAFFAQRFGTTRVLLITGAWAAIVPFVMLPL
VHVGTPLSITVGVGLATLAESGYYSVVAGFVSGIFVARIRYTAISIAYQVCGALAGGLTPLVATIIAQNTAPQWWPLAIQ
YTSAAVLSSLCVWLISRRVSIDDAGSSAKDVDALPRAGAHTAARTA
>Mature_446_residues
METSVKQPKRAALASFVGTTIEWYDFYSYATAAAIVFGPLFFPGENRFVSLLASFGSFAVGFFARPFGGVLFGYLGDRFG
RKRSLLATLMLMAVSTVAIGLLPTYATAGMLAPILLVLMRVLQGIAVGGEWGGAVLLAGEHAPAGKRTFFASFAQLGSAS
GLILSMLAFGAISTLPKEDLMSWGWRLPFLASAVLLAVGFVIRASVSESPEFEEVRKSGDTARNPVREALKYWPLLLLAI
GANVYGIAGVYFSNIFMISYATQFLSLDRSMVLHCMTIVAVLQFVVQLAAAFFAQRFGTTRVLLITGAWAAIVPFVMLPL
VHVGTPLSITVGVGLATLAESGYYSVVAGFVSGIFVARIRYTAISIAYQVCGALAGGLTPLVATIIAQNTAPQWWPLAIQ
YTSAAVLSSLCVWLISRRVSIDDAGSSAKDVDALPRAGAHTAARTA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=420, Percent_Identity=37.8571428571429, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1789941, Length=395, Percent_Identity=37.4683544303797, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1790550, Length=403, Percent_Identity=31.2655086848635, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1788942, Length=387, Percent_Identity=30.2325581395349, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082231, Length=168, Percent_Identity=26.7857142857143, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47377; Mature: 47377

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSVKQPKRAALASFVGTTIEWYDFYSYATAAAIVFGPLFFPGENRFVSLLASFGSFAV
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GFFARPFGGVLFGYLGDRFGRKRSLLATLMLMAVSTVAIGLLPTYATAGMLAPILLVLMR
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQGIAVGGEWGGAVLLAGEHAPAGKRTFFASFAQLGSASGLILSMLAFGAISTLPKEDL
HHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHH
MSWGWRLPFLASAVLLAVGFVIRASVSESPEFEEVRKSGDTARNPVREALKYWPLLLLAI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GANVYGIAGVYFSNIFMISYATQFLSLDRSMVLHCMTIVAVLQFVVQLAAAFFAQRFGTT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVLLITGAWAAIVPFVMLPLVHVGTPLSITVGVGLATLAESGYYSVVAGFVSGIFVARIR
EEEEEECCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YTAISIAYQVCGALAGGLTPLVATIIAQNTAPQWWPLAIQYTSAAVLSSLCVWLISRRVS
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC
IDDAGSSAKDVDALPRAGAHTAARTA
CCCCCCCCHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
METSVKQPKRAALASFVGTTIEWYDFYSYATAAAIVFGPLFFPGENRFVSLLASFGSFAV
CCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GFFARPFGGVLFGYLGDRFGRKRSLLATLMLMAVSTVAIGLLPTYATAGMLAPILLVLMR
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQGIAVGGEWGGAVLLAGEHAPAGKRTFFASFAQLGSASGLILSMLAFGAISTLPKEDL
HHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHH
MSWGWRLPFLASAVLLAVGFVIRASVSESPEFEEVRKSGDTARNPVREALKYWPLLLLAI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GANVYGIAGVYFSNIFMISYATQFLSLDRSMVLHCMTIVAVLQFVVQLAAAFFAQRFGTT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVLLITGAWAAIVPFVMLPLVHVGTPLSITVGVGLATLAESGYYSVVAGFVSGIFVARIR
EEEEEECCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YTAISIAYQVCGALAGGLTPLVATIIAQNTAPQWWPLAIQYTSAAVLSSLCVWLISRRVS
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC
IDDAGSSAKDVDALPRAGAHTAARTA
CCCCCCCCHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]