Click here to switch to the map view.

The map label for this gene is hcaD [H]

Identifier: 189353718

GI number: 189353718

Start: 1983785

End: 1984933

Strand: Reverse

Name: hcaD [H]

Synonym: BMULJ_04963

Alternate gene names: 189353718

Gene position: 1984933-1983785 (Counterclockwise)

Preceding gene: 189353719

Following gene: 189353717

Centisome position: 80.26

GC content: 66.58

Gene sequence:

>1149_bases
ATGATGCGAGATGCGGCCAATTTCGAGCCGACCGACAGCCGGGTCACGCGCCCGGCTTTCTGGAACGCCCTTGCCGAGCG
CTGGACGAGCGACGTCGAGGAAACGCTGGTGTGCTGCCACGTGCGGCAGGAAACGCACGACGTGAAGAGCTTCTTCTTCC
GTTCGCCGCAGGGCCGCACGTTCTCGTTCGAGCCCGGCCAGTTCATCACGCTCGAGCTCGACATCGACGGCGAGACGATC
AATCGCTGCTACACGATCTCGTCGTCGCCGACGCGGCCGCACACCATCTCGATCACCGTCAAGCGCGTGCCGGGCGGCAA
GGTGTCGAACTGGCTGCACGACAACCTGCAGCCGGGCGCGTCGGTTCGCGTGCTCGGGCCGGCCGGCGAGTTCACCTGCG
CGCGGCATCCGGCGCGCAAGTACCTGTTCCTGTCCGCGGGCTCGGGCGTCACGCCGCTGATGTCGATGAGCCGCGCGCAT
CACGATCTCGCCGAGGATCGCGACATCGTGTTCGTGCACAGCGCACGCACGCCCGACGACATCATCTTCGCGCGCGAGCT
CGATCTGATCGCGTCGAACCACACGAATTTCCGCACGTCGTTCGTCGTCGAACGCGTCGGCACGCGCACGAACTGGCCGG
GCGTCACCGGCTTCCTGACGCTGCCGCTGCTCAAGCTGATCGCACCGGACTTCATGGAGCGCGAGATCTTCACGTGCGGC
CCGGCGCCCTACATGAAGGCCGTGCGCGACCTGCTCGACGAAGCCGGTTTCGACCGCGAGCGCTATCACGAGGAAAGCTT
CTCGTTCGAGACGCTCGCGCAGACGGCGAGCGACGAACTGCTCGCCGAGCTCGCGCCCGTGGCCGACGAGGCCGAGGTCG
CGACGAAGCAGTTCACGGTCAGCTTCGCGAAGAGCAACCGCGAGGTGTCGTGCGGCGCCGACCAGCACGTGCTCGATGCG
GCGCGCCGCTCGGGCGTGCGGCTGCCGGCCTCGTGCACGCAGGGCATGTGCGGCACCTGCAAGGTGAAGCTCGTGTCGGG
GCAGGTCGACATGAAGCACAACGGCGGGATTCGCCAGCGCGAGATCGACCAGGGCATGGTGCTGCTGTGCTGCAGCAAGC
CGCTTTCGGATCTCGTCATCGATAAATAA

Upstream 100 bases:

>100_bases
TCACGGAAGGGCTCGTCGAGAAGTTCTCGGCGTGGTACATCGGCCGGCTCGCCGAAAAGACCGGCGCATAGCGATTAGCG
ATTTAGCAGCGGAGTCAAAC

Downstream 100 bases:

>100_bases
CGACTGGCCGCGATGCGACGCGCCGCGTCGCATCCGGACAAGCATCTGTCGGAAAAAAACGCTACCGCGGATTTGTGGTT
GAAGAACCTAGGGAAACGCT

Product: ferredoxin-NAD+ reductase

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MMRDAANFEPTDSRVTRPAFWNALAERWTSDVEETLVCCHVRQETHDVKSFFFRSPQGRTFSFEPGQFITLELDIDGETI
NRCYTISSSPTRPHTISITVKRVPGGKVSNWLHDNLQPGASVRVLGPAGEFTCARHPARKYLFLSAGSGVTPLMSMSRAH
HDLAEDRDIVFVHSARTPDDIIFARELDLIASNHTNFRTSFVVERVGTRTNWPGVTGFLTLPLLKLIAPDFMEREIFTCG
PAPYMKAVRDLLDEAGFDRERYHEESFSFETLAQTASDELLAELAPVADEAEVATKQFTVSFAKSNREVSCGADQHVLDA
ARRSGVRLPASCTQGMCGTCKVKLVSGQVDMKHNGGIRQREIDQGMVLLCCSKPLSDLVIDK

Sequences:

>Translated_382_residues
MMRDAANFEPTDSRVTRPAFWNALAERWTSDVEETLVCCHVRQETHDVKSFFFRSPQGRTFSFEPGQFITLELDIDGETI
NRCYTISSSPTRPHTISITVKRVPGGKVSNWLHDNLQPGASVRVLGPAGEFTCARHPARKYLFLSAGSGVTPLMSMSRAH
HDLAEDRDIVFVHSARTPDDIIFARELDLIASNHTNFRTSFVVERVGTRTNWPGVTGFLTLPLLKLIAPDFMEREIFTCG
PAPYMKAVRDLLDEAGFDRERYHEESFSFETLAQTASDELLAELAPVADEAEVATKQFTVSFAKSNREVSCGADQHVLDA
ARRSGVRLPASCTQGMCGTCKVKLVSGQVDMKHNGGIRQREIDQGMVLLCCSKPLSDLVIDK
>Mature_382_residues
MMRDAANFEPTDSRVTRPAFWNALAERWTSDVEETLVCCHVRQETHDVKSFFFRSPQGRTFSFEPGQFITLELDIDGETI
NRCYTISSSPTRPHTISITVKRVPGGKVSNWLHDNLQPGASVRVLGPAGEFTCARHPARKYLFLSAGSGVTPLMSMSRAH
HDLAEDRDIVFVHSARTPDDIIFARELDLIASNHTNFRTSFVVERVGTRTNWPGVTGFLTLPLLKLIAPDFMEREIFTCG
PAPYMKAVRDLLDEAGFDRERYHEESFSFETLAQTASDELLAELAPVADEAEVATKQFTVSFAKSNREVSCGADQHVLDA
ARRSGVRLPASCTQGMCGTCKVKLVSGQVDMKHNGGIRQREIDQGMVLLCCSKPLSDLVIDK

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI84875541, Length=238, Percent_Identity=28.1512605042017, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1787098, Length=344, Percent_Identity=32.8488372093023, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787658, Length=356, Percent_Identity=28.6516853932584, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788104, Length=343, Percent_Identity=23.6151603498542, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788903, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323552, Length=194, Percent_Identity=26.8041237113402, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322146, Length=223, Percent_Identity=25.5605381165919, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322699, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323510, Length=178, Percent_Identity=26.4044943820225, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 42517; Mature: 42517

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRDAANFEPTDSRVTRPAFWNALAERWTSDVEETLVCCHVRQETHDVKSFFFRSPQGRT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FSFEPGQFITLELDIDGETINRCYTISSSPTRPHTISITVKRVPGGKVSNWLHDNLQPGA
EEECCCCEEEEEEEECCCEECEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCC
SVRVLGPAGEFTCARHPARKYLFLSAGSGVTPLMSMSRAHHDLAEDRDIVFVHSARTPDD
EEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
IIFARELDLIASNHTNFRTSFVVERVGTRTNWPGVTGFLTLPLLKLIAPDFMEREIFTCG
EEEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCEECC
PAPYMKAVRDLLDEAGFDRERYHEESFSFETLAQTASDELLAELAPVADEAEVATKQFTV
CHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEE
SFAKSNREVSCGADQHVLDAARRSGVRLPASCTQGMCGTCKVKLVSGQVDMKHNGGIRQR
EEECCCCEEECCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEECEEEECCCCCCCHH
EIDQGMVLLCCSKPLSDLVIDK
ECCCCEEEEEECCCHHHHCCCC
>Mature Secondary Structure
MMRDAANFEPTDSRVTRPAFWNALAERWTSDVEETLVCCHVRQETHDVKSFFFRSPQGRT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
FSFEPGQFITLELDIDGETINRCYTISSSPTRPHTISITVKRVPGGKVSNWLHDNLQPGA
EEECCCCEEEEEEEECCCEECEEEEECCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCC
SVRVLGPAGEFTCARHPARKYLFLSAGSGVTPLMSMSRAHHDLAEDRDIVFVHSARTPDD
EEEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
IIFARELDLIASNHTNFRTSFVVERVGTRTNWPGVTGFLTLPLLKLIAPDFMEREIFTCG
EEEEEHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCEECC
PAPYMKAVRDLLDEAGFDRERYHEESFSFETLAQTASDELLAELAPVADEAEVATKQFTV
CHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEE
SFAKSNREVSCGADQHVLDAARRSGVRLPASCTQGMCGTCKVKLVSGQVDMKHNGGIRQR
EEECCCCEEECCCCHHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEECEEEECCCCCCCHH
EIDQGMVLLCCSKPLSDLVIDK
ECCCCEEEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA