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The map label for this gene is paaG [H]

Identifier: 189353612

GI number: 189353612

Start: 1869389

End: 1870528

Strand: Reverse

Name: paaG [H]

Synonym: BMULJ_04856

Alternate gene names: 189353612

Gene position: 1870528-1869389 (Counterclockwise)

Preceding gene: 189353613

Following gene: 189353610

Centisome position: 75.63

GC content: 68.86

Gene sequence:

>1140_bases
ATGAGCACGCCGGCCACGAACCAGGATGCCTTCACCGATCCCGCGCAGGACGTGCTGTTTCGCGTGGTGAACCGCGTCGC
GTTGATCACGCTCAATCGTCCGTCCGCGCTGAACGCGCTGTCGTACGAGATGGTGCGCGCGCTTGCCGCGCTATTCGAAC
GCTGCCGCAACGACGACCGCATCGTCGCGGTCGTGCTGCGCGGTGCCGGCGACAAGGGTTTCTGCGCGGGCGGCGACGTG
CGGCAGCTGCATCGGATGGTCGCGCAGCGTGAAACGTGGCTGCCGTTCTTCGTCGACGAATACCGGCTCGACCATGCGAT
CCATACGTTTCCGAAGCCGGTCGTCGCGCTGATGGACGGCGTGACGATGGGCGGCGGCATGGGGCTCGCGCAGGGTGCCG
CGCTGCGCGTCGCGACCGAGCGCAGCAAGATCGCGATGCCGGAAACGCGCATCGGCCTCGTGCCGGATGTCGGCGCGACG
CATTTTCTCGCGCGGATGCCCGTCGAACTCGAGCTGTACGTCGGCCTCACCGGTGCGACGCTGTCGGGTGCCGATGCGCT
GAGCGCGAAGCTCGCCGACCTCTGCGTGCCGTCGTCGTGGCTCGACACGTTCGAGACGCGCATCGAGCGCGTCAACTGGG
ACGGCGATGCGCTGCCCGCGCTGCGCAAGGTGTTCGAGCCGCCGTGCAACGTCGTCCCGCACGCGGCGCTCGACACGCAA
CTGCCGTGGATCGTCCGGCACTTCGACAAGCGTTCGCCGGTCGAGCGGATCGTCGCGACGCTGAAGCAGGACCTCGCGCG
CGACGAGCTGTCGCGCGAACAGCGTCAGTGGCTGCAGTCGACGCTCGACGCGCTGATGGGCCATTCGCCGACGATGCTGG
CCGTCACGCGCGAAGCGCTGCTGCGCGGCCGGCAGATGACGCTCGCGGAGTCGTTCCGGATGGAGCTCGGCATCGTCGCG
CGCGCGATCGAGGAAGGCGACTTCTGCGAAGGCGTGCGCGCGCATCTGATCGACAAGGATCGCAAGCCGCGCTGGGCGCC
CGCGACGCTCGTCGAGCTGCGTGCCGAACGCGTGCGGCATTTCCTGACGTCGCCGTGGAAGCTGTTCGCGCATCCGCTCG
CCGATCTCGGCGCCGCGTGA

Upstream 100 bases:

>100_bases
GCTTCGTCGACCTGTTCGACACGAACGATCCGCGCGAAGGCGTGGCCGCGTTTCTCGGCAAGCGCGCACCGCAATGGCAA
CCCGAAGGAGAGCAGTCACG

Downstream 100 bases:

>100_bases
TCGCGCGCGCCCGACGTGCCGCGTCGCGCGTCGGGTGTCGTCTGCGTTTGCGTCATGCGTTTGCCGGAGCGCGCTCGATC
ATCGGCGGCACGGCCTCCAC

Product: enoyl-CoA hydratase

Products: Trans-2(Or 3)-Enoyl- CoA; H2O. 3-Oxoacyl-CoA; NADH. (R)-3-Hydroxybutanoyl-CoA. [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSTPATNQDAFTDPAQDVLFRVVNRVALITLNRPSALNALSYEMVRALAALFERCRNDDRIVAVVLRGAGDKGFCAGGDV
RQLHRMVAQRETWLPFFVDEYRLDHAIHTFPKPVVALMDGVTMGGGMGLAQGAALRVATERSKIAMPETRIGLVPDVGAT
HFLARMPVELELYVGLTGATLSGADALSAKLADLCVPSSWLDTFETRIERVNWDGDALPALRKVFEPPCNVVPHAALDTQ
LPWIVRHFDKRSPVERIVATLKQDLARDELSREQRQWLQSTLDALMGHSPTMLAVTREALLRGRQMTLAESFRMELGIVA
RAIEEGDFCEGVRAHLIDKDRKPRWAPATLVELRAERVRHFLTSPWKLFAHPLADLGAA

Sequences:

>Translated_379_residues
MSTPATNQDAFTDPAQDVLFRVVNRVALITLNRPSALNALSYEMVRALAALFERCRNDDRIVAVVLRGAGDKGFCAGGDV
RQLHRMVAQRETWLPFFVDEYRLDHAIHTFPKPVVALMDGVTMGGGMGLAQGAALRVATERSKIAMPETRIGLVPDVGAT
HFLARMPVELELYVGLTGATLSGADALSAKLADLCVPSSWLDTFETRIERVNWDGDALPALRKVFEPPCNVVPHAALDTQ
LPWIVRHFDKRSPVERIVATLKQDLARDELSREQRQWLQSTLDALMGHSPTMLAVTREALLRGRQMTLAESFRMELGIVA
RAIEEGDFCEGVRAHLIDKDRKPRWAPATLVELRAERVRHFLTSPWKLFAHPLADLGAA
>Mature_378_residues
STPATNQDAFTDPAQDVLFRVVNRVALITLNRPSALNALSYEMVRALAALFERCRNDDRIVAVVLRGAGDKGFCAGGDVR
QLHRMVAQRETWLPFFVDEYRLDHAIHTFPKPVVALMDGVTMGGGMGLAQGAALRVATERSKIAMPETRIGLVPDVGATH
FLARMPVELELYVGLTGATLSGADALSAKLADLCVPSSWLDTFETRIERVNWDGDALPALRKVFEPPCNVVPHAALDTQL
PWIVRHFDKRSPVERIVATLKQDLARDELSREQRQWLQSTLDALMGHSPTMLAVTREALLRGRQMTLAESFRMELGIVAR
AIEEGDFCEGVRAHLIDKDRKPRWAPATLVELRAERVRHFLTSPWKLFAHPLADLGAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=361, Percent_Identity=35.7340720221607, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI37594469, Length=312, Percent_Identity=34.9358974358974, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI194097323, Length=179, Percent_Identity=35.195530726257, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI68989263, Length=219, Percent_Identity=30.5936073059361, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788682, Length=183, Percent_Identity=30.6010928961749, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788597, Length=152, Percent_Identity=28.2894736842105, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI221142681, Length=223, Percent_Identity=26.0089686098655, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787659, Length=175, Percent_Identity=29.7142857142857, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787660, Length=151, Percent_Identity=34.4370860927152, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=343, Percent_Identity=39.067055393586, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI25144157, Length=343, Percent_Identity=39.067055393586, Blast_Score=230, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI17540714, Length=181, Percent_Identity=29.2817679558011, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=137, Percent_Identity=35.036496350365, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=163, Percent_Identity=32.5153374233129, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=352, Percent_Identity=31.8181818181818, Blast_Score=155, Evalue=7e-39,
Organism=Drosophila melanogaster, GI28571730, Length=363, Percent_Identity=38.0165289256198, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI28571729, Length=363, Percent_Identity=38.0165289256198, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24653139, Length=142, Percent_Identity=35.9154929577465, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI20129971, Length=178, Percent_Identity=30.3370786516854, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653477, Length=178, Percent_Identity=30.3370786516854, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17; 1.1.1.35; 5.1.2.3 [C]

Molecular weight: Translated: 42069; Mature: 41938

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPATNQDAFTDPAQDVLFRVVNRVALITLNRPSALNALSYEMVRALAALFERCRNDDR
CCCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
IVAVVLRGAGDKGFCAGGDVRQLHRMVAQRETWLPFFVDEYRLDHAIHTFPKPVVALMDG
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCC
VTMGGGMGLAQGAALRVATERSKIAMPETRIGLVPDVGATHFLARMPVELELYVGLTGAT
CCCCCCCCHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCC
LSGADALSAKLADLCVPSSWLDTFETRIERVNWDGDALPALRKVFEPPCNVVPHAALDTQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHCCC
LPWIVRHFDKRSPVERIVATLKQDLARDELSREQRQWLQSTLDALMGHSPTMLAVTREAL
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRGRQMTLAESFRMELGIVARAIEEGDFCEGVRAHLIDKDRKPRWAPATLVELRAERVRH
HCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
FLTSPWKLFAHPLADLGAA
HHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STPATNQDAFTDPAQDVLFRVVNRVALITLNRPSALNALSYEMVRALAALFERCRNDDR
CCCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
IVAVVLRGAGDKGFCAGGDVRQLHRMVAQRETWLPFFVDEYRLDHAIHTFPKPVVALMDG
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCC
VTMGGGMGLAQGAALRVATERSKIAMPETRIGLVPDVGATHFLARMPVELELYVGLTGAT
CCCCCCCCHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCC
LSGADALSAKLADLCVPSSWLDTFETRIERVNWDGDALPALRKVFEPPCNVVPHAALDTQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHCCC
LPWIVRHFDKRSPVERIVATLKQDLARDELSREQRQWLQSTLDALMGHSPTMLAVTREAL
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRGRQMTLAESFRMELGIVARAIEEGDFCEGVRAHLIDKDRKPRWAPATLVELRAERVRH
HCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
FLTSPWKLFAHPLADLGAA
HHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3s)-3-Hydroxyacyl-CoA L-3-Hydroxyacyl-CoA; NAD(+) (S)-3-Hydroxybutanoyl-CoA [C]

Specific reaction: (3s)-3-Hydroxyacyl-CoA = Trans-2(Or 3)-Enoyl- CoA + H2O. L-3-Hydroxyacyl-CoA + NAD(+) = 3-Oxoacyl-CoA + NADH. (S)-3-Hydroxybutanoyl-CoA = (R)-3-Hydroxybutanoyl-CoA. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]