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The map label for this gene is bltL [H]

Identifier: 189353610

GI number: 189353610

Start: 1866699

End: 1868237

Strand: Reverse

Name: bltL [H]

Synonym: BMULJ_04854

Alternate gene names: 189353610

Gene position: 1868237-1866699 (Counterclockwise)

Preceding gene: 189353612

Following gene: 189353607

Centisome position: 75.54

GC content: 69.4

Gene sequence:

>1539_bases
ATGACGCTATCCGAATCCCGCTGGAATGCGGTCGCGCTGGCGGCCGTCTGTCTCACATCGCTGATGTTCGGCCTCGAAAT
CTCGAGCGTGCCGGTGATCCTGCCGACGCTCGAACACGTGCTGCATGGCGACTTCAACGGCATGCAGTGGATCATGAATG
CGTACACGATCGCGTGTACGACCGTGCTGATGGCCGCCGGTACGCTCGCGGACCGCTTCGGCCGCAAGCGCGTGTACGTG
ATCGGCACCGCGCTGTTCGGCGCGACGTCGCTGCTGTGCGGGCTTGCGCCGAACGTGCCGGTGCTCGTCGCCGGCCGGCT
GCTGCAGGGTGCGAGCGGCGGCGCGATGCTGATCTGCCAGATCGCCGTGCTGTCGCACCAGTTTCGCGAAGGGCGCGAGC
GCGGCCGCGCATTCGGCATCTGGGGGATCGTGTTCGGGATCGGCCTCGGCTTCGGGCCGATCGTCGGCGGCGCGATCGTC
GCGGTCGCGAGCTGGCCGTGGGTGTTTCTCGTGCATGCGCCGCTCGCGGCCGTCGCACTCGCGCTGATCGGCGGCGCCGT
GCAGGAGTCGCGCGACCCGCATGCGGGCACGCTCGACGTCGCGGGCATCGTCACGCTGTCGCTCGCGGTGCTCGGCCTCG
CGTTCTATATCACGCAGAGTGCGGCGCTCGGGCTCACGAGCGCGGCCGGCCTCGGCGTGCTCGGTGCGACCGTGCTCGCG
GTCGGCGCGTTCGTCGTCGTGGAGCGCAGAAGTGCGCGGCCGATGTTCGACTTCTCGGTATTCCGCATTCGCGCGTTCAC
CGGCGCGATCTTCGGCTCGATGGGGATGAACTTCAGCTTCTGGCCGTTCATGATCTATCTGCCGATCTGGTTTCAGGTCG
CGCTCGGTTACGACAGCGTGACGGCTGGCCTCGCGCTGCTCGCCTATACGCTGCCGACGCTCGTCGCGCCGCCGTTCGGC
GAGCGGCTCGCGTTGCGCTACGGGCCCGGCGTGGTGATTCCGGGCGGCCTGTTCACGATCGCGGCCGGGTTTGCGCTGAT
GCGGATCGGCAGCGCGGCCGAACACGCAAGCTGGCTCACGATGCTGCCCGGCTGCGTGATCGCCGGCATCGGGCTCGGGC
TCGCCAACACGCCGGTCACGAACACGACGACCGGTGCGGTGCCGAGCGCACGGGCCGGCATGGCGTCGGGTATCGACATG
AGCGCGCGGATGATTTCGCTCGCGCTGAACATCGCGATGATGGGCTTCGTGCTCGTGGCGGGAATCGTCGCGAACCTGAA
GGGCGGCGTTGCAGCGGCGATCGGCGATGCATCGCTGCGCGCGCTCGCGCAGGAAATCGCTGCGGGCCGCACGGAAGATT
TGCGTGCGATCGCGCCGGCGCTCACGCAGGCCGACCCGACCGGCGCGGCGCTGCATGCGGCGCTCGTGCATGGGTTCGGC
TGGGTGATGGTGTACGGTGCGGCGGGTGTGGCCGTGCTGGCGACCGCGAGTGCCATCGCGTTCGCACCGACGCGGCGCAG
CGTGGCCGTCTGCGAGTGA

Upstream 100 bases:

>100_bases
ATAGTACCGATTCCCGTCCAGCGTGCGGCGCCGGCGCGCACGCGGCGAATCCTTCCGACCTGGAGGCGCGCGACTCGGCG
ACGGGCCGCGCGCAATACGC

Downstream 100 bases:

>100_bases
GCGCGCGAGCGGTTGGCTGTGCTGCCGTGTGGCCCGCGTTGTGTGCCGGCTTCGATGCCGGTTATGGGCGGTGCGTGTGC
GTTTGTGTTTGCGATGACGT

Product: multidrug resistance protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTLSESRWNAVALAAVCLTSLMFGLEISSVPVILPTLEHVLHGDFNGMQWIMNAYTIACTTVLMAAGTLADRFGRKRVYV
IGTALFGATSLLCGLAPNVPVLVAGRLLQGASGGAMLICQIAVLSHQFREGRERGRAFGIWGIVFGIGLGFGPIVGGAIV
AVASWPWVFLVHAPLAAVALALIGGAVQESRDPHAGTLDVAGIVTLSLAVLGLAFYITQSAALGLTSAAGLGVLGATVLA
VGAFVVVERRSARPMFDFSVFRIRAFTGAIFGSMGMNFSFWPFMIYLPIWFQVALGYDSVTAGLALLAYTLPTLVAPPFG
ERLALRYGPGVVIPGGLFTIAAGFALMRIGSAAEHASWLTMLPGCVIAGIGLGLANTPVTNTTTGAVPSARAGMASGIDM
SARMISLALNIAMMGFVLVAGIVANLKGGVAAAIGDASLRALAQEIAAGRTEDLRAIAPALTQADPTGAALHAALVHGFG
WVMVYGAAGVAVLATASAIAFAPTRRSVAVCE

Sequences:

>Translated_512_residues
MTLSESRWNAVALAAVCLTSLMFGLEISSVPVILPTLEHVLHGDFNGMQWIMNAYTIACTTVLMAAGTLADRFGRKRVYV
IGTALFGATSLLCGLAPNVPVLVAGRLLQGASGGAMLICQIAVLSHQFREGRERGRAFGIWGIVFGIGLGFGPIVGGAIV
AVASWPWVFLVHAPLAAVALALIGGAVQESRDPHAGTLDVAGIVTLSLAVLGLAFYITQSAALGLTSAAGLGVLGATVLA
VGAFVVVERRSARPMFDFSVFRIRAFTGAIFGSMGMNFSFWPFMIYLPIWFQVALGYDSVTAGLALLAYTLPTLVAPPFG
ERLALRYGPGVVIPGGLFTIAAGFALMRIGSAAEHASWLTMLPGCVIAGIGLGLANTPVTNTTTGAVPSARAGMASGIDM
SARMISLALNIAMMGFVLVAGIVANLKGGVAAAIGDASLRALAQEIAAGRTEDLRAIAPALTQADPTGAALHAALVHGFG
WVMVYGAAGVAVLATASAIAFAPTRRSVAVCE
>Mature_511_residues
TLSESRWNAVALAAVCLTSLMFGLEISSVPVILPTLEHVLHGDFNGMQWIMNAYTIACTTVLMAAGTLADRFGRKRVYVI
GTALFGATSLLCGLAPNVPVLVAGRLLQGASGGAMLICQIAVLSHQFREGRERGRAFGIWGIVFGIGLGFGPIVGGAIVA
VASWPWVFLVHAPLAAVALALIGGAVQESRDPHAGTLDVAGIVTLSLAVLGLAFYITQSAALGLTSAAGLGVLGATVLAV
GAFVVVERRSARPMFDFSVFRIRAFTGAIFGSMGMNFSFWPFMIYLPIWFQVALGYDSVTAGLALLAYTLPTLVAPPFGE
RLALRYGPGVVIPGGLFTIAAGFALMRIGSAAEHASWLTMLPGCVIAGIGLGLANTPVTNTTTGAVPSARAGMASGIDMS
ARMISLALNIAMMGFVLVAGIVANLKGGVAAAIGDASLRALAQEIAAGRTEDLRAIAPALTQADPTGAALHAALVHGFGW
VMVYGAAGVAVLATASAIAFAPTRRSVAVCE

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=380, Percent_Identity=29.4736842105263, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=24.1379310344828, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788710, Length=318, Percent_Identity=25.1572327044025, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789042, Length=321, Percent_Identity=23.3644859813084, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788392, Length=316, Percent_Identity=26.8987341772152, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=270, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319770, Length=290, Percent_Identity=25.5172413793103, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=151, Percent_Identity=28.476821192053, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52527; Mature: 52395

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSESRWNAVALAAVCLTSLMFGLEISSVPVILPTLEHVLHGDFNGMQWIMNAYTIACT
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TVLMAAGTLADRFGRKRVYVIGTALFGATSLLCGLAPNVPVLVAGRLLQGASGGAMLICQ
HHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHH
IAVLSHQFREGRERGRAFGIWGIVFGIGLGFGPIVGGAIVAVASWPWVFLVHAPLAAVAL
HHHHHHHHHHHHHHCCEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ALIGGAVQESRDPHAGTLDVAGIVTLSLAVLGLAFYITQSAALGLTSAAGLGVLGATVLA
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHH
VGAFVVVERRSARPMFDFSVFRIRAFTGAIFGSMGMNFSFWPFMIYLPIWFQVALGYDSV
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHH
TAGLALLAYTLPTLVAPPFGERLALRYGPGVVIPGGLFTIAAGFALMRIGSAAEHASWLT
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHHHHHHH
MLPGCVIAGIGLGLANTPVTNTTTGAVPSARAGMASGIDMSARMISLALNIAMMGFVLVA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GIVANLKGGVAAAIGDASLRALAQEIAAGRTEDLRAIAPALTQADPTGAALHAALVHGFG
HHHHHCCCCCEEHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
WVMVYGAAGVAVLATASAIAFAPTRRSVAVCE
HHHHHHHCHHHHHHHHHHHEECCCCCCEECCC
>Mature Secondary Structure 
TLSESRWNAVALAAVCLTSLMFGLEISSVPVILPTLEHVLHGDFNGMQWIMNAYTIACT
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH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HHHHHHHCHHHHHHHHHHHEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]