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The map label for this gene is actII-2 [H]

Identifier: 189353466

GI number: 189353466

Start: 1685406

End: 1686869

Strand: Direct

Name: actII-2 [H]

Synonym: BMULJ_04710

Alternate gene names: 189353466

Gene position: 1685406-1686869 (Clockwise)

Preceding gene: 189353464

Following gene: 189353468

Centisome position: 68.15

GC content: 72.13

Gene sequence:

>1464_bases
ATGACGGACAACCTTCTTGCCCGCTGCGCGCCCGCCGGCGCCGCGCCGGCGGCCACGCCGCACAATCGCCACGGCTGGGC
GCTCGTCGTGCTGCTGGTCGGCGCGATCCTGCCGCCGCTCGACTACTTCATCGTCAACCTTGCGCTGCCGGCGATCCGCG
ACGGCATCGGCGCGCGGCCGGCCGAGCTGCAGCTCGTCGTGTCGGCGTACGCCTGCGCGAACGCGGTTGCGCAGATCACC
GGCGGCCGTCTCGGCGACCTGTACGGCCGCAAGCGGATGTTCATGCTCGGCATGGCGGGCTTCGTCGTCGCCTCGACGCT
GTGCGGGCTCGCCGACAGCGGCGCCGTGCTGGTCGGCGGTCGCGTGCTGCAGGGCCTGTTCGCGGCGATCCTCGCGCCGC
AGGTGCTCGCGACGATCCGCAGCGTGTTCACGCCGCAGGAGCAGGTGCGCGTGATGGGCTTCTACGGCTTCGTGTTCGGG
CTCGCGGCGGTGATCGGCCAGCTCGGCGGCGGCGCGCTGATCGACCTGCATCCGTTCGGGCTCGGCTGGCGCGCGATCTT
CCTCGTCAATCTGCCGATCGGCGTACTTGCGCTGCTCGGCAGCTGGCGCTTCATCCCGGAAAACCGGCCGCCGCGCGGCC
AGCGCATCGACGTGCCGGGCACCGTGCTGCTGTCGCTGTTCCTGCTGATGCTCGTGTACCCGCTCACGCACGGGCGCGAC
GCCGGCTGGCCCGCGTGGATGGTCGCGTGCGCGATCGGCGCGCTGCCGGTGCTCGCGGCGCTGCTCGCCGTCGAAGCACG
CCAACTCGCCGCCGGCCGCGATCCGCTGCTCGACCTGCGGCTGTTCCGGCATCCCGTCGTCGCGCTCGGTCTCGCGCTTG
CGTTCCTGTTCTACATGCTGAGCGCGTTCTTCCTCAGCTACGGCATCTATCTGCAGGGATGCCTGCACTGGTCGCCGCTC
GCGTCGGGCGCGGCGATCCTGCCGCTCGGCATCGGCTTTCTGTCGAGCCCGCTGCTGATGCCGCGGCTCGTCGGACGCTT
CGGCGGCTATCGCGTGCTCACGCTCGGCTTCGCGATGCTTGCGGCAGGCGTCGCCGCAGCGGCCGCGCTGGCCGGCAGCG
GCGCGCCGGGAATCGGCTTCTATGCGGGCATCGCCGCGATCGGCATCGGACAGGGCCTCGTGCTGCCGTCCGTCGTGCGG
ATCGTGCTCGCCGAAGTCGATGCGTCGCGCGCGGGTGTTGCCTCCGGGATGGTCACGGCGGCACTGCAGATCGGTGCGGC
CGTCGGCGCCGCGACGCTCGGCGGCATGTTCTTCGCGCAGCTCGGCGCGGCGCCGGGCCCGCTCGACTACGTACGCGGGT
TCCGCACGTCGATGTTTGCGCTGAGCGCGATCCTCGCCGTGTGCGTCGTGCTGTCGGCCGCGCTCGGGCCGATCCACCGA
CGGCTGCATGCCGCGGCCGCATAA

Upstream 100 bases:

>100_bases
GATGGATACTACCACCATTTCCCGCTTTTCCGAATCATCGACGATGCGCACAATGTCCGCTCGACGGCCGCTTGCGGCCT
CTTTTCTCTGGACTTCCTCA

Downstream 100 bases:

>100_bases
GCGCGCGGCGGGCGCAGCCGATGCGTCAACGATGCGATTCGGGTTTCGCGTGCCGCGCTTCCCATGCGGCGAGCGCGGCG
CGATATTCGTCCATCGCCTC

Product: permease of the major facilitator superfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTDNLLARCAPAGAAPAATPHNRHGWALVVLLVGAILPPLDYFIVNLALPAIRDGIGARPAELQLVVSAYACANAVAQIT
GGRLGDLYGRKRMFMLGMAGFVVASTLCGLADSGAVLVGGRVLQGLFAAILAPQVLATIRSVFTPQEQVRVMGFYGFVFG
LAAVIGQLGGGALIDLHPFGLGWRAIFLVNLPIGVLALLGSWRFIPENRPPRGQRIDVPGTVLLSLFLLMLVYPLTHGRD
AGWPAWMVACAIGALPVLAALLAVEARQLAAGRDPLLDLRLFRHPVVALGLALAFLFYMLSAFFLSYGIYLQGCLHWSPL
ASGAAILPLGIGFLSSPLLMPRLVGRFGGYRVLTLGFAMLAAGVAAAAALAGSGAPGIGFYAGIAAIGIGQGLVLPSVVR
IVLAEVDASRAGVASGMVTAALQIGAAVGAATLGGMFFAQLGAAPGPLDYVRGFRTSMFALSAILAVCVVLSAALGPIHR
RLHAAAA

Sequences:

>Translated_487_residues
MTDNLLARCAPAGAAPAATPHNRHGWALVVLLVGAILPPLDYFIVNLALPAIRDGIGARPAELQLVVSAYACANAVAQIT
GGRLGDLYGRKRMFMLGMAGFVVASTLCGLADSGAVLVGGRVLQGLFAAILAPQVLATIRSVFTPQEQVRVMGFYGFVFG
LAAVIGQLGGGALIDLHPFGLGWRAIFLVNLPIGVLALLGSWRFIPENRPPRGQRIDVPGTVLLSLFLLMLVYPLTHGRD
AGWPAWMVACAIGALPVLAALLAVEARQLAAGRDPLLDLRLFRHPVVALGLALAFLFYMLSAFFLSYGIYLQGCLHWSPL
ASGAAILPLGIGFLSSPLLMPRLVGRFGGYRVLTLGFAMLAAGVAAAAALAGSGAPGIGFYAGIAAIGIGQGLVLPSVVR
IVLAEVDASRAGVASGMVTAALQIGAAVGAATLGGMFFAQLGAAPGPLDYVRGFRTSMFALSAILAVCVVLSAALGPIHR
RLHAAAA
>Mature_486_residues
TDNLLARCAPAGAAPAATPHNRHGWALVVLLVGAILPPLDYFIVNLALPAIRDGIGARPAELQLVVSAYACANAVAQITG
GRLGDLYGRKRMFMLGMAGFVVASTLCGLADSGAVLVGGRVLQGLFAAILAPQVLATIRSVFTPQEQVRVMGFYGFVFGL
AAVIGQLGGGALIDLHPFGLGWRAIFLVNLPIGVLALLGSWRFIPENRPPRGQRIDVPGTVLLSLFLLMLVYPLTHGRDA
GWPAWMVACAIGALPVLAALLAVEARQLAAGRDPLLDLRLFRHPVVALGLALAFLFYMLSAFFLSYGIYLQGCLHWSPLA
SGAAILPLGIGFLSSPLLMPRLVGRFGGYRVLTLGFAMLAAGVAAAAALAGSGAPGIGFYAGIAAIGIGQGLVLPSVVRI
VLAEVDASRAGVASGMVTAALQIGAAVGAATLGGMFFAQLGAAPGPLDYVRGFRTSMFALSAILAVCVVLSAALGPIHRR
LHAAAA

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=414, Percent_Identity=28.9855072463768, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1788710, Length=350, Percent_Identity=29.1428571428571, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788392, Length=435, Percent_Identity=25.0574712643678, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=24.7572815533981, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789042, Length=341, Percent_Identity=24.9266862170088, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790146, Length=180, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50177; Mature: 50045

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNLLARCAPAGAAPAATPHNRHGWALVVLLVGAILPPLDYFIVNLALPAIRDGIGARP
CCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
AELQLVVSAYACANAVAQITGGRLGDLYGRKRMFMLGMAGFVVASTLCGLADSGAVLVGG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECH
RVLQGLFAAILAPQVLATIRSVFTPQEQVRVMGFYGFVFGLAAVIGQLGGGALIDLHPFG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LGWRAIFLVNLPIGVLALLGSWRFIPENRPPRGQRIDVPGTVLLSLFLLMLVYPLTHGRD
CCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCC
AGWPAWMVACAIGALPVLAALLAVEARQLAAGRDPLLDLRLFRHPVVALGLALAFLFYML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SAFFLSYGIYLQGCLHWSPLASGAAILPLGIGFLSSPLLMPRLVGRFGGYRVLTLGFAML
HHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
AAGVAAAAALAGSGAPGIGFYAGIAAIGIGQGLVLPSVVRIVLAEVDASRAGVASGMVTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALQIGAAVGAATLGGMFFAQLGAAPGPLDYVRGFRTSMFALSAILAVCVVLSAALGPIHR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLHAAAA
HHHHHCC
>Mature Secondary Structure 
TDNLLARCAPAGAAPAATPHNRHGWALVVLLVGAILPPLDYFIVNLALPAIRDGIGARP
CCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCC
AELQLVVSAYACANAVAQITGGRLGDLYGRKRMFMLGMAGFVVASTLCGLADSGAVLVGG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECH
RVLQGLFAAILAPQVLATIRSVFTPQEQVRVMGFYGFVFGLAAVIGQLGGGALIDLHPFG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
LGWRAIFLVNLPIGVLALLGSWRFIPENRPPRGQRIDVPGTVLLSLFLLMLVYPLTHGRD
CCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCC
AGWPAWMVACAIGALPVLAALLAVEARQLAAGRDPLLDLRLFRHPVVALGLALAFLFYML
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SAFFLSYGIYLQGCLHWSPLASGAAILPLGIGFLSSPLLMPRLVGRFGGYRVLTLGFAML
HHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
AAGVAAAAALAGSGAPGIGFYAGIAAIGIGQGLVLPSVVRIVLAEVDASRAGVASGMVTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALQIGAAVGAATLGGMFFAQLGAAPGPLDYVRGFRTSMFALSAILAVCVVLSAALGPIHR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLHAAAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]