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The map label for this gene is hipO [H]

Identifier: 189353295

GI number: 189353295

Start: 1532916

End: 1534079

Strand: Reverse

Name: hipO [H]

Synonym: BMULJ_04535

Alternate gene names: 189353295

Gene position: 1534079-1532916 (Counterclockwise)

Preceding gene: 189353297

Following gene: 189353291

Centisome position: 62.03

GC content: 65.03

Gene sequence:

>1164_bases
ATGGAAAGCACACTGCAATCGAAGTTGAAACATTGGCGGCATCACTTTCACCGCCATCCCGAGACGGGCTTCGAGGAAGT
CGCGACGTCCGACTACGTCGCGGCGCTTCTGGAAAGCTTCGGGCTCGACGTGCATCGCGGCATCGGCGGCACCGGCGTCG
TCGCCAACCTGAAGGTGGGCGAGAGTCCGCGCGCGATCGGCTTGCGCGCCGACATGGACGCGCTGAACATCGTCGAGAAC
GTACCGGCGCGCGCGCATGCGTCGTGCGTGCCGGGCAAGATGCATGCATGCGGACACGACGGCCATATGGCGATGGTGCT
CGGCGCGGCGCGCTTGCTCGCCGAGCAACGCGATTTCGACGGAACCGTGCGGTTCATTTTTCAGCCGGCGGAAGAGCACG
GGCGCGGCGCGAAGGCGATGATGGCCGACGGGCTGTTCGAGCGCTTTCCGATCGACGCGATCTTCGGCGCGCACAACATG
CCCGGGATGCCGGCCGGATCGTTCTCGACGCGTGCCGGCGGCATCATGGCGAGCGAAGACAATTTCGTGATTCGGATCAA
CGCGCGCGGCACGCATGCGGCGCGCCCGCATATGGGCGTCGATCCGATCGTGATCGCGGCGCAGATCGTGCTCGCGCTGC
AGACGATCATTTCGCGCAATCTCGATCCGAGCCTGCCTGCAGTGATTTCGTGCACGGAGATCCTGACCGACGGGCTGCGC
AACGTGATTCCGTCGACGGTAACGATCAAAGGCGACACGCGCAGCTATTCGCCTGAGGTGCAGCAGCTGCTGGCGACGCG
GATGCGCGAAGTGAGCGAGGGAATCTGCCGGATGCATGGCGTCGAATGCGAATTCGCGTACACGCACGAGTTCGCGCCGA
CCGTCAATTCGCCGGAATACGTGGAGGTCGCGGTGGCGGCGGCGACGCGCGTCGCGGGCGCACTGTACGTCGATGCGAAC
GTGCAGCCGATGATGATTTCGGAAGACTTCGGCGCGTTCCTGCAGGTCGTCCCCGGCAATTTCATCTTCATCGGCAACGG
GAGCGCGGGCGAGCGCGGCGGCGTGCCGCTGCACAACGCGACGTACGACTTCAACGACGACATCCTGCCGCTGGGCGCGC
GGTACTTTGCCGAAGTGGCGCGCATTGCGCTCGCAAGCGCCTGA

Upstream 100 bases:

>100_bases
TGACGCGGGCGTGTCGGGCTCAGAAGGTCCGCGCGTATCGGGCGACGCAAGTGCGCGGCCGGCGCCGGTGCCATTCGGCG
ATTTGACGGATAGCGCGAGC

Downstream 100 bases:

>100_bases
CGACGGCGCAGCGGTGGGGCGGCTTGCCGCTGCCGGTGCCGCACCGAGCCGGCGCGGGCAATAAAAAAGCCCGCTGCTGG
AGCGGGCCGAAACCATGTTT

Product: hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MESTLQSKLKHWRHHFHRHPETGFEEVATSDYVAALLESFGLDVHRGIGGTGVVANLKVGESPRAIGLRADMDALNIVEN
VPARAHASCVPGKMHACGHDGHMAMVLGAARLLAEQRDFDGTVRFIFQPAEEHGRGAKAMMADGLFERFPIDAIFGAHNM
PGMPAGSFSTRAGGIMASEDNFVIRINARGTHAARPHMGVDPIVIAAQIVLALQTIISRNLDPSLPAVISCTEILTDGLR
NVIPSTVTIKGDTRSYSPEVQQLLATRMREVSEGICRMHGVECEFAYTHEFAPTVNSPEYVEVAVAAATRVAGALYVDAN
VQPMMISEDFGAFLQVVPGNFIFIGNGSAGERGGVPLHNATYDFNDDILPLGARYFAEVARIALASA

Sequences:

>Translated_387_residues
MESTLQSKLKHWRHHFHRHPETGFEEVATSDYVAALLESFGLDVHRGIGGTGVVANLKVGESPRAIGLRADMDALNIVEN
VPARAHASCVPGKMHACGHDGHMAMVLGAARLLAEQRDFDGTVRFIFQPAEEHGRGAKAMMADGLFERFPIDAIFGAHNM
PGMPAGSFSTRAGGIMASEDNFVIRINARGTHAARPHMGVDPIVIAAQIVLALQTIISRNLDPSLPAVISCTEILTDGLR
NVIPSTVTIKGDTRSYSPEVQQLLATRMREVSEGICRMHGVECEFAYTHEFAPTVNSPEYVEVAVAAATRVAGALYVDAN
VQPMMISEDFGAFLQVVPGNFIFIGNGSAGERGGVPLHNATYDFNDDILPLGARYFAEVARIALASA
>Mature_387_residues
MESTLQSKLKHWRHHFHRHPETGFEEVATSDYVAALLESFGLDVHRGIGGTGVVANLKVGESPRAIGLRADMDALNIVEN
VPARAHASCVPGKMHACGHDGHMAMVLGAARLLAEQRDFDGTVRFIFQPAEEHGRGAKAMMADGLFERFPIDAIFGAHNM
PGMPAGSFSTRAGGIMASEDNFVIRINARGTHAARPHMGVDPIVIAAQIVLALQTIISRNLDPSLPAVISCTEILTDGLR
NVIPSTVTIKGDTRSYSPEVQQLLATRMREVSEGICRMHGVECEFAYTHEFAPTVNSPEYVEVAVAAATRVAGALYVDAN
VQPMMISEDFGAFLQVVPGNFIFIGNGSAGERGGVPLHNATYDFNDDILPLGARYFAEVARIALASA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=428, Percent_Identity=28.9719626168224, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787598, Length=188, Percent_Identity=33.5106382978723, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41610; Mature: 41610

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTLQSKLKHWRHHFHRHPETGFEEVATSDYVAALLESFGLDVHRGIGGTGVVANLKVG
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEEEEC
ESPRAIGLRADMDALNIVENVPARAHASCVPGKMHACGHDGHMAMVLGAARLLAEQRDFD
CCCCEEEEECCCHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GTVRFIFQPAEEHGRGAKAMMADGLFERFPIDAIFGAHNMPGMPAGSFSTRAGGIMASED
CEEEEEEECCHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEECCC
NFVIRINARGTHAARPHMGVDPIVIAAQIVLALQTIISRNLDPSLPAVISCTEILTDGLR
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
NVIPSTVTIKGDTRSYSPEVQQLLATRMREVSEGICRMHGVECEFAYTHEFAPTVNSPEY
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCH
VEVAVAAATRVAGALYVDANVQPMMISEDFGAFLQVVPGNFIFIGNGSAGERGGVPLHNA
HHHHHHHHHHHHEEEEECCCCCEEEECCHHHHHHEECCCCEEEEECCCCCCCCCCCCCCC
TYDFNDDILPLGARYFAEVARIALASA
CCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESTLQSKLKHWRHHFHRHPETGFEEVATSDYVAALLESFGLDVHRGIGGTGVVANLKVG
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEEEEEC
ESPRAIGLRADMDALNIVENVPARAHASCVPGKMHACGHDGHMAMVLGAARLLAEQRDFD
CCCCEEEEECCCHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
GTVRFIFQPAEEHGRGAKAMMADGLFERFPIDAIFGAHNMPGMPAGSFSTRAGGIMASED
CEEEEEEECCHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCEEECCC
NFVIRINARGTHAARPHMGVDPIVIAAQIVLALQTIISRNLDPSLPAVISCTEILTDGLR
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
NVIPSTVTIKGDTRSYSPEVQQLLATRMREVSEGICRMHGVECEFAYTHEFAPTVNSPEY
HHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCH
VEVAVAAATRVAGALYVDANVQPMMISEDFGAFLQVVPGNFIFIGNGSAGERGGVPLHNA
HHHHHHHHHHHHEEEEECCCCCEEEECCHHHHHHEECCCCEEEEECCCCCCCCCCCCCCC
TYDFNDDILPLGARYFAEVARIALASA
CCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]