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The map label for this gene is cyaE [H]

Identifier: 189353289

GI number: 189353289

Start: 1527199

End: 1528740

Strand: Direct

Name: cyaE [H]

Synonym: BMULJ_04529

Alternate gene names: 189353289

Gene position: 1527199-1528740 (Clockwise)

Preceding gene: 189353288

Following gene: 189353290

Centisome position: 61.75

GC content: 72.57

Gene sequence:

>1542_bases
ATGCGCCGACCTGAACCGCCGGCCGCGTCGATCGTCGCAGCCGCCCTCGCAAGCGCCGTCGCGCTGGCCGGCTGCGCGAC
CTCGTCGATCGATCTCGCGCCCAACGCATCGGACCGCCCGTGGCAGCCGCAGACCTCGGCCGCGGGCGAGATCCTCGCGG
CACCGCCGCACGCATCCGCGACGCCGCACGACTACACGCTGCCGGCCACGCCCGCGCTCGCGTCGGTGTCGCCGCCCGCC
GCGCTGGATGCCGCGCACGCGTACACGCTGCCCGAACTGATCGACCTCGCGGAATCGACCAACCCGCTGACGCGCATCGC
GTGGAACGACGCGCGCAACGCGGCGCTCGCGGTCGGTATCGCGAAGGCGGCCTACCTGCCGCAGCTGTCGGCGACCGCGA
TGGGCGCGTACCAGACCGGCCACGGCTCGACGTCGACAGTCCTCGGCGACGCGTCGAGCGACACCACCGTGCACGGCACG
ATCTCCGCGCTGTCGCTGCAATGGCTGCTGTTCGACTTCGGCGGTCGCGCGGCGCGCGTCGAGGCGGCCGAACAGGCGTC
GATCGCGTCGAACGTCGCGTTCACGGCCGTGCACCAGCAGGTGATCCACGATGTGACCGTCGCCTACTACCGCTACGAAG
CCGCGCGCTCGCGCGCGCTCAGCGCGCAGCAAGGGCTCGCGAATGCCGATGCGATCCTCGCGGCGTCGCGCGCACGGCTC
CGGCAGGGCGTCGGCACCGTCGTCGAGCTCGCGCAGGCCACGCAGAATCGCGCGCAGGCGAACCTCGCGCTCGTGCAGGC
GCGCGGCGCGGAAAGCGACAGCTACCTCGCCCTCGTCTCCGCGCTCGGCATTTCGCCGTTGTCGAAGCCGACCATCGCCG
CGCTGCCCGTGCGGCCGCTGCCGCCCGCGCTCGGTTCATCGGTCGATGCCATCGTCGCCGATGCGATCGCGCGCCGCCCC
GACCTGCAGGGCGCGTATGCGCTCGAGAAGGCTAACCGCGCAAAGATCAAGGCCGCCGAGGCCGCGTTCATGCCGAAGGT
GTTCCTGTCCGCGTCGACGTCGTATGCGAGCGGGCACACGGCCGTCTCCGCACTGCCCGCGATCGGCGACCAGGCGCCGA
CCGTGAACCTGAACGGCAGCCGCTACGGCGCGGGCGTGTTCGTCGGCGTGACGATTCCGCTGTACGACGGCGGCCTGCGC
TCGGCCGTGCTGATGCAGGCGCACAACGACGCGCGAAGCGCAACCGAACGGCTGACGCGCACGAAGGAGGAAGCCGTGCG
CCAGGTCGTCGCCGCGCAGAACGCGGTGCAGACGAGCCTCGCGTCGCACGATGCCGCGAAGGCGCTCGTCGATGCCGCGC
AGACGAGCTACGACGCGGCACTGACCGCGTACCGCAGCGGCGTCGGCTCGATCACCGATGCGACGCTCGCGCAGAGCCAG
CTGCTCGCCGCGCGCAACGCCGAGGTCGACAGCTATGCCGGCGCGCTGTCGGCGGCCGCCGCGCTCGCGCTCGCGACGGG
GACGATCGGTTCGGCGCAGTGA

Upstream 100 bases:

>100_bases
CCCGACCCTGCGCGCGACGCACTGCTGAACCTCATCGACGCGCGACTCGCCGCCATCGCCGACGCCGCGCGCCAAGCCAC
TTCGAAGGAGTCTGCCGCTC

Downstream 100 bases:

>100_bases
CGCCCGCGGCCCACAACGGCCTGCCCGCGTTCCGCCGTCGCGGCGGTTGACGCCCGAGCGCGCCGCCCACTAGAATGCCG
CGCATTCTCCCTTCAGGAAC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MRRPEPPAASIVAAALASAVALAGCATSSIDLAPNASDRPWQPQTSAAGEILAAPPHASATPHDYTLPATPALASVSPPA
ALDAAHAYTLPELIDLAESTNPLTRIAWNDARNAALAVGIAKAAYLPQLSATAMGAYQTGHGSTSTVLGDASSDTTVHGT
ISALSLQWLLFDFGGRAARVEAAEQASIASNVAFTAVHQQVIHDVTVAYYRYEAARSRALSAQQGLANADAILAASRARL
RQGVGTVVELAQATQNRAQANLALVQARGAESDSYLALVSALGISPLSKPTIAALPVRPLPPALGSSVDAIVADAIARRP
DLQGAYALEKANRAKIKAAEAAFMPKVFLSASTSYASGHTAVSALPAIGDQAPTVNLNGSRYGAGVFVGVTIPLYDGGLR
SAVLMQAHNDARSATERLTRTKEEAVRQVVAAQNAVQTSLASHDAAKALVDAAQTSYDAALTAYRSGVGSITDATLAQSQ
LLAARNAEVDSYAGALSAAAALALATGTIGSAQ

Sequences:

>Translated_513_residues
MRRPEPPAASIVAAALASAVALAGCATSSIDLAPNASDRPWQPQTSAAGEILAAPPHASATPHDYTLPATPALASVSPPA
ALDAAHAYTLPELIDLAESTNPLTRIAWNDARNAALAVGIAKAAYLPQLSATAMGAYQTGHGSTSTVLGDASSDTTVHGT
ISALSLQWLLFDFGGRAARVEAAEQASIASNVAFTAVHQQVIHDVTVAYYRYEAARSRALSAQQGLANADAILAASRARL
RQGVGTVVELAQATQNRAQANLALVQARGAESDSYLALVSALGISPLSKPTIAALPVRPLPPALGSSVDAIVADAIARRP
DLQGAYALEKANRAKIKAAEAAFMPKVFLSASTSYASGHTAVSALPAIGDQAPTVNLNGSRYGAGVFVGVTIPLYDGGLR
SAVLMQAHNDARSATERLTRTKEEAVRQVVAAQNAVQTSLASHDAAKALVDAAQTSYDAALTAYRSGVGSITDATLAQSQ
LLAARNAEVDSYAGALSAAAALALATGTIGSAQ
>Mature_513_residues
MRRPEPPAASIVAAALASAVALAGCATSSIDLAPNASDRPWQPQTSAAGEILAAPPHASATPHDYTLPATPALASVSPPA
ALDAAHAYTLPELIDLAESTNPLTRIAWNDARNAALAVGIAKAAYLPQLSATAMGAYQTGHGSTSTVLGDASSDTTVHGT
ISALSLQWLLFDFGGRAARVEAAEQASIASNVAFTAVHQQVIHDVTVAYYRYEAARSRALSAQQGLANADAILAASRARL
RQGVGTVVELAQATQNRAQANLALVQARGAESDSYLALVSALGISPLSKPTIAALPVRPLPPALGSSVDAIVADAIARRP
DLQGAYALEKANRAKIKAAEAAFMPKVFLSASTSYASGHTAVSALPAIGDQAPTVNLNGSRYGAGVFVGVTIPLYDGGLR
SAVLMQAHNDARSATERLTRTKEEAVRQVVAAQNAVQTSLASHDAAKALVDAAQTSYDAALTAYRSGVGSITDATLAQSQ
LLAARNAEVDSYAGALSAAAALALATGTIGSAQ

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=432, Percent_Identity=24.0740740740741, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52203; Mature: 52203

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRPEPPAASIVAAALASAVALAGCATSSIDLAPNASDRPWQPQTSAAGEILAAPPHASA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEEECCCCCCC
TPHDYTLPATPALASVSPPAALDAAHAYTLPELIDLAESTNPLTRIAWNDARNAALAVGI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHH
AKAAYLPQLSATAMGAYQTGHGSTSTVLGDASSDTTVHGTISALSLQWLLFDFGGRAARV
HHHHHCCCHHHHHHCCEECCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCEEEH
EAAEQASIASNVAFTAVHQQVIHDVTVAYYRYEAARSRALSAQQGLANADAILAASRARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RQGVGTVVELAQATQNRAQANLALVQARGAESDSYLALVSALGISPLSKPTIAALPVRPL
HHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCC
PPALGSSVDAIVADAIARRPDLQGAYALEKANRAKIKAAEAAFMPKVFLSASTSYASGHT
CHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AVSALPAIGDQAPTVNLNGSRYGAGVFVGVTIPLYDGGLRSAVLMQAHNDARSATERLTR
HHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHH
TKEEAVRQVVAAQNAVQTSLASHDAAKALVDAAQTSYDAALTAYRSGVGSITDATLAQSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LLAARNAEVDSYAGALSAAAALALATGTIGSAQ
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRPEPPAASIVAAALASAVALAGCATSSIDLAPNASDRPWQPQTSAAGEILAAPPHASA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEEECCCCCCC
TPHDYTLPATPALASVSPPAALDAAHAYTLPELIDLAESTNPLTRIAWNDARNAALAVGI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHH
AKAAYLPQLSATAMGAYQTGHGSTSTVLGDASSDTTVHGTISALSLQWLLFDFGGRAARV
HHHHHCCCHHHHHHCCEECCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCEEEH
EAAEQASIASNVAFTAVHQQVIHDVTVAYYRYEAARSRALSAQQGLANADAILAASRARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RQGVGTVVELAQATQNRAQANLALVQARGAESDSYLALVSALGISPLSKPTIAALPVRPL
HHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCC
PPALGSSVDAIVADAIARRPDLQGAYALEKANRAKIKAAEAAFMPKVFLSASTSYASGHT
CHHHCCCHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AVSALPAIGDQAPTVNLNGSRYGAGVFVGVTIPLYDGGLRSAVLMQAHNDARSATERLTR
HHHHHHCCCCCCCEEECCCCCCCCEEEEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHH
TKEEAVRQVVAAQNAVQTSLASHDAAKALVDAAQTSYDAALTAYRSGVGSITDATLAQSQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LLAARNAEVDSYAGALSAAAALALATGTIGSAQ
HHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]