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The map label for this gene is emrB [C]

Identifier: 189353240

GI number: 189353240

Start: 1474171

End: 1475685

Strand: Direct

Name: emrB [C]

Synonym: BMULJ_04480

Alternate gene names: 189353240

Gene position: 1474171-1475685 (Clockwise)

Preceding gene: 189353238

Following gene: 189353241

Centisome position: 59.61

GC content: 69.17

Gene sequence:

>1515_bases
ATGCAAACCGATACCCTTCCCTCGCCGTCCGTCGATACCGTCGCGCGCCGCGATGCGCCGACGGCGCCGATCATGGCGGC
GCTGCTGCTCGTGATGCTGTTGTCCGCGCTCGATCAGACGATCGTGTCGACCGCACTGCCGACGATCGTCGGCGAACTCG
GCGGGCTCGACCGGCTGTCGTGGGTCGTGACCGCGTACCTGCTGTCGTCGACCGTCGTGCTGCCGCTGTACGGCAAGCTC
GGCGATCTGTACGGGCGCAAGATCGTGCTGCAGGTCGCGATCGCGCTGTTCCTCGCGGGATCGGCGCTCTGCGGAATCGC
GCAGGACATGACGCAGCTGATCGCGCTGCGCGCGCTGCAGGGCCTCGGCGGCGGCGGGTTGATGGTCGTCACGATGGCTG
CGATCGGCGATCTCGTCCCGCCCGACCGCCGCGCGCGCTATCAGGGGATGTTCGGCGGCGTCTACGGGCTCGCGACGATC
GTCGGGCCGCTGCTCGGCGGTTTTCTCGTGGAGCATCTGTCGTGGCGCTGGATCTTCACGATCAACCTGCCGCTCGGCTT
TCTCGCGCTCGCCGTGATCGGCATCGCATTCCGGCCGCATGCCGCGCACGTGAAGCACCGCATCGACTACCTGGGCGCCG
CGTTTCTCGCGACCGCGCTCACCTGCGTGATCCTGTTTACGAGCGAAGGCGGCTCGTTGCTGCCGTGGTCGTCGCCGCAG
CTCTGGATGACGCTCGTGCTCGGGCTCGTCGCGATCGGCGGGTTCGTGTACGAGGAGCGGCTCGCGGCCGAGCCGATCAT
GCCGCTCGAGCTGTTCCGGCATCGCACCTTCGTGCTGTGCTGCGCGATCGGCTTCGTCGTCGGCGTCGCGCTGTTCGGCT
CGGTCACGTTCATTCCGCTCTATCTGCAGGTCGTGAAAGGCTCGACGCCGTCGCAGGCCGGCATGCAGCTGCTGCCGATG
ATGGGCGGGATGCTCGCGATGTCGGTCGCAAGCGGCCGGCTGATCGCGCGACTCGGCTCGTACCGCGCGTTTCCGATCGC
CGGCACGCTGATCGGCGGCGTCGCGATGGTGCTGCTGTCGACGCTGTCGCTCGACACGCCGCTGCACACGATGTACGCGT
ACATGGCGCTGCTCGGCGTCGGCCTCGGGATGGTGATGCCCGTGCTCACGCTCGCCGCGCAGAACACCGTCGAGTTCCGG
CACATGGGCGTCGCGACGTCCGGCGCGACGCTGTTCCGCTCGATCGGCGGCTCGATCGGCGTCGCCGCGTTCGGTGCGCT
GTTCTCGCACGGCCTGCGCGCGCGGCTGATCGATGCGCTGCCGACCGGCACCGAACTGCCGGCGGCGCTCGGTCCCGGCA
CGCTCGGGCAGCTGCCCGAGGCGGTACGCAGCGCATACCTGCATGCGTTCGCCGGCTCGCTGCACGTCGTCTATCTGGCC
GCGGCGACGGTGATCGCGATCGCGTTCGTGCTTGCCTGGTTCGTCGAGGATGCACCGCTGCGCACGCGCGGCTGA

Upstream 100 bases:

>100_bases
GCACCGTTACGACTGCAACAATTCGAGCCCAAGCAGAAATTGACTAGTCATCTATCGACCACTATATTTCGAGCAACTTC
CTGCCATGGCAGATAACGCA

Downstream 100 bases:

>100_bases
CGGATGCGGCGGCGCCGCTTTGCGCGGCCGCCGACACGCGGGCCGGCGCCGCGCGCGCCGATCATGCAGCAGGCGCCGGT
GCCGTCCGCTTTACCTGCCG

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MQTDTLPSPSVDTVARRDAPTAPIMAALLLVMLLSALDQTIVSTALPTIVGELGGLDRLSWVVTAYLLSSTVVLPLYGKL
GDLYGRKIVLQVAIALFLAGSALCGIAQDMTQLIALRALQGLGGGGLMVVTMAAIGDLVPPDRRARYQGMFGGVYGLATI
VGPLLGGFLVEHLSWRWIFTINLPLGFLALAVIGIAFRPHAAHVKHRIDYLGAAFLATALTCVILFTSEGGSLLPWSSPQ
LWMTLVLGLVAIGGFVYEERLAAEPIMPLELFRHRTFVLCCAIGFVVGVALFGSVTFIPLYLQVVKGSTPSQAGMQLLPM
MGGMLAMSVASGRLIARLGSYRAFPIAGTLIGGVAMVLLSTLSLDTPLHTMYAYMALLGVGLGMVMPVLTLAAQNTVEFR
HMGVATSGATLFRSIGGSIGVAAFGALFSHGLRARLIDALPTGTELPAALGPGTLGQLPEAVRSAYLHAFAGSLHVVYLA
AATVIAIAFVLAWFVEDAPLRTRG

Sequences:

>Translated_504_residues
MQTDTLPSPSVDTVARRDAPTAPIMAALLLVMLLSALDQTIVSTALPTIVGELGGLDRLSWVVTAYLLSSTVVLPLYGKL
GDLYGRKIVLQVAIALFLAGSALCGIAQDMTQLIALRALQGLGGGGLMVVTMAAIGDLVPPDRRARYQGMFGGVYGLATI
VGPLLGGFLVEHLSWRWIFTINLPLGFLALAVIGIAFRPHAAHVKHRIDYLGAAFLATALTCVILFTSEGGSLLPWSSPQ
LWMTLVLGLVAIGGFVYEERLAAEPIMPLELFRHRTFVLCCAIGFVVGVALFGSVTFIPLYLQVVKGSTPSQAGMQLLPM
MGGMLAMSVASGRLIARLGSYRAFPIAGTLIGGVAMVLLSTLSLDTPLHTMYAYMALLGVGLGMVMPVLTLAAQNTVEFR
HMGVATSGATLFRSIGGSIGVAAFGALFSHGLRARLIDALPTGTELPAALGPGTLGQLPEAVRSAYLHAFAGSLHVVYLA
AATVIAIAFVLAWFVEDAPLRTRG
>Mature_504_residues
MQTDTLPSPSVDTVARRDAPTAPIMAALLLVMLLSALDQTIVSTALPTIVGELGGLDRLSWVVTAYLLSSTVVLPLYGKL
GDLYGRKIVLQVAIALFLAGSALCGIAQDMTQLIALRALQGLGGGGLMVVTMAAIGDLVPPDRRARYQGMFGGVYGLATI
VGPLLGGFLVEHLSWRWIFTINLPLGFLALAVIGIAFRPHAAHVKHRIDYLGAAFLATALTCVILFTSEGGSLLPWSSPQ
LWMTLVLGLVAIGGFVYEERLAAEPIMPLELFRHRTFVLCCAIGFVVGVALFGSVTFIPLYLQVVKGSTPSQAGMQLLPM
MGGMLAMSVASGRLIARLGSYRAFPIAGTLIGGVAMVLLSTLSLDTPLHTMYAYMALLGVGLGMVMPVLTLAAQNTVEFR
HMGVATSGATLFRSIGGSIGVAAFGALFSHGLRARLIDALPTGTELPAALGPGTLGQLPEAVRSAYLHAFAGSLHVVYLA
AATVIAIAFVLAWFVEDAPLRTRG

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=319, Percent_Identity=30.0940438871473, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1790195, Length=432, Percent_Identity=29.8611111111111, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788392, Length=408, Percent_Identity=31.3725490196078, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081983, Length=308, Percent_Identity=29.2207792207792, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788710, Length=321, Percent_Identity=26.1682242990654, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1790146, Length=152, Percent_Identity=30.9210526315789, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=331, Percent_Identity=31.7220543806647, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322958, Length=481, Percent_Identity=23.4927234927235, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319770, Length=471, Percent_Identity=25.0530785562633, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325455, Length=519, Percent_Identity=23.121387283237, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321663, Length=172, Percent_Identity=28.4883720930233, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324954, Length=374, Percent_Identity=25.9358288770053, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319783, Length=377, Percent_Identity=22.0159151193634, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52897; Mature: 52897

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTDTLPSPSVDTVARRDAPTAPIMAALLLVMLLSALDQTIVSTALPTIVGELGGLDRLS
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
WVVTAYLLSSTVVLPLYGKLGDLYGRKIVLQVAIALFLAGSALCGIAQDMTQLIALRALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGGGGLMVVTMAAIGDLVPPDRRARYQGMFGGVYGLATIVGPLLGGFLVEHLSWRWIFT
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
INLPLGFLALAVIGIAFRPHAAHVKHRIDYLGAAFLATALTCVILFTSEGGSLLPWSSPQ
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHH
LWMTLVLGLVAIGGFVYEERLAAEPIMPLELFRHRTFVLCCAIGFVVGVALFGSVTFIPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQVVKGSTPSQAGMQLLPMMGGMLAMSVASGRLIARLGSYRAFPIAGTLIGGVAMVLLS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
TLSLDTPLHTMYAYMALLGVGLGMVMPVLTLAAQNTVEFRHMGVATSGATLFRSIGGSIG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCHH
VAAFGALFSHGLRARLIDALPTGTELPAALGPGTLGQLPEAVRSAYLHAFAGSLHVVYLA
HHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AATVIAIAFVLAWFVEDAPLRTRG
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTDTLPSPSVDTVARRDAPTAPIMAALLLVMLLSALDQTIVSTALPTIVGELGGLDRLS
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
WVVTAYLLSSTVVLPLYGKLGDLYGRKIVLQVAIALFLAGSALCGIAQDMTQLIALRALQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLGGGGLMVVTMAAIGDLVPPDRRARYQGMFGGVYGLATIVGPLLGGFLVEHLSWRWIFT
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
INLPLGFLALAVIGIAFRPHAAHVKHRIDYLGAAFLATALTCVILFTSEGGSLLPWSSPQ
EECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHH
LWMTLVLGLVAIGGFVYEERLAAEPIMPLELFRHRTFVLCCAIGFVVGVALFGSVTFIPL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLQVVKGSTPSQAGMQLLPMMGGMLAMSVASGRLIARLGSYRAFPIAGTLIGGVAMVLLS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHH
TLSLDTPLHTMYAYMALLGVGLGMVMPVLTLAAQNTVEFRHMGVATSGATLFRSIGGSIG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHCCCHH
VAAFGALFSHGLRARLIDALPTGTELPAALGPGTLGQLPEAVRSAYLHAFAGSLHVVYLA
HHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AATVIAIAFVLAWFVEDAPLRTRG
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]